somite unit chronometry to analyze teratogen phase specificity in the paraxial mesoderm
Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: fine-tuning of autophagy in Caenorhabditis elegans homeostasis doc
Ngày tải lên :
29/03/2014, 08:20
... signaling, which is the key inhibitory signaling for autophagy, responding to growth factors and amino acid signaling, (b) AMP-activated protein kinase, which activates autophagy through the inhibition ... diagram of autophagy signaling pathway in C elegans Autophagy inducing- and inhibiting signaling pathway in C elegans Plain lines and dashed lines indicate known and possible regulatory pathways, ... death-associated protein kinase (DAPK) in the regulation of autophagy Fine-tuning of autophagy in C elegans homeostasis is particularly interesting DAPK is a serine ⁄ threonine kinase originally isolated...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
Ngày tải lên :
06/08/2014, 19:21
... pathways Amino-acid pools and other nutrients activate TOR signaling, which then results in increased translation following activation of the protein kinase S6K and the translation initiation factor ... upregulated in these mutants [8] Life-span-prolonging effects of downregulating protein synthesis might be specific to C elegans and other invertebrates The soma of the adult nematode is postmitotic, ... life-span determination l Second, branched-chain amino acids are known to stimulate protein synthesis and inhibit protein degrada tion in higher eukaryotes [10], a phenomenon mediated by the TOR (target...
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Báo cáo sinh học: "Robust and specific inhibition of microRNAs in Caenorhabditis elegans" doc
Ngày tải lên :
06/08/2014, 19:21
... yet the phenotype observed in the miR-84 knockdown animals was completely unlike the let-7 knockdown phenotype therefore confirming the specificity of inhibition Using a similar strategy, the ... inhibition against target miRNAs in the animal The inhibition was robust until 15-20 hours after the L4 molt with higher doses of injection, after which the effect diminished, most probably due to the ... rhodamine-labeled antisense oligonucleotide complementary to the target microRNA is injected into the syncytial gonads of C. elegans The transformed progeny are selected by the presence of rhodamine...
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Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 01:20
... the functions of these uridine kinase homologs, we analyzed their enzyme activity in vitro The proteins purified from the bacterial induction system and several substrates were incubated together, ... to the culture Induced fusion proteins were purified using amylose resin (New England Biolabs), according to the manufacturer’s protocol, and the UPP-1 proteins were cleaved and eluted by factor ... strongly in neurons and the pharynx, but weakly in the intestine Scale bars: 100 lm (B) In vitro enzymatic assays of three uridine kinase homologs using analytical HPLC No proteins exhibited uridine...
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Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 04:20
... against polyhistidine revealed the same-sized bands, suggesting that the C-terminal histidine tag remained in both the proform and activated form in the recombinant enzyme after its secretion into ... during development Using an antibody against the prodomain, we previously demonstrated that the MIG-17 proform localizes to the surfaces of the gonad, intestine and hypodermis corresponding to the ... which to study the function of ADAMTSs during organogenesis in vivo In this study, we isolated recombinant MIG-17 and determined the amino acid sequence of the N-terminal neo-epitope of the activated...
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Báo cáo sinh học: "A global analysis of genetic interactions in Caenorhabditis elegans" doc
Ngày tải lên :
06/08/2014, 18:21
... of the pathways analyzed involve receptor tyrosine kinase signaling Interestingly, let-60 and sem-5 each interact with all of the query genes but not interact with clk-2, suggesting that they ... interact in either study Of the 28 pairs found to interact by Lehner et al., 18 also interact in the SGI network There are no obvious differences in the phenotypes of the 18 interacting gene ... examining fat accumulation in C elegans is to incubate worms in Nile Red vital dye, which stains lipids and readily accumulates within the triglyceride deposits in the intestine [46] We therefore...
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Báo cáo y học: "Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails" ppsx
Ngày tải lên :
14/08/2014, 14:21
... budding yeast (cutoff BLASTP score E = × 10-20) in each bin is indicated as in (a) In all panels, the red dotted line indicates the average of all the genes, and the blue dotted lines indicate the ... contains the legends to the above three tables The primers tothe sensory the intestine,and results of the microarray experiments Genes listing additional threefor Click here datathethose of the ... but not in neurons or the intestine, while 'Intestine' was genes expressed in the intestine, but not in neurons or muscles Xboxes were searched for using MEME and MAST [60] based on the definition...
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Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
Ngày tải lên :
14/08/2014, 17:22
... dsRNA-expressing bacterial strain targeting lin-31 (data not shown), showing that the observed dilution effect appears not to be specific to the diluting dsRNA-expressing strain interactions Taken together ... to determine whether we could adapt these methods, which are efficient for analyzing the RNAi phenotypes of single genes, to targeting multiple genes by combinatorial RNAi To investigate whether ... research The duplicated genes that we focused on in the worm correspond to single genes in either S cerevisiae or D melanogaster genomes We wished to investigate whether the known function of the single...
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Báo cáo y học: "Identification of ciliary and ciliopathy genes in Caenorhabditis elegans through comparative genomics" pptx
Ngày tải lên :
14/08/2014, 17:22
... mining and gene finding HMMER and motif finding The engineering procedure was as described in our previous publications [53,54] Briefly, the GFP coding sequence was 'stitched' together with the ... 2), suggesting that these X-box-containing C elegans genes contain significantly (p = 7.6 × e-9, Fisher's interactions To further ascertain whether the X-box-containing genes identified in Additional ... need to have a more detailed understanding of the properties of X-box motifs, including the variation, preferred position in the promoter, and interaction with other binding motifs Some of these...
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Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt
Ngày tải lên :
05/03/2014, 23:20
... tachykinin receptors in the gut, with special reference to NK2 receptors in human Auton Neurosci 126–127, 232–249 32 Burnstock G (2008) The journey to establish purinergic signalling in the gut Neurogastroenterol ... suggesting that prejunctional P2 receptors were not involved in the facilitating effects of cADPR In contrast, the inhibitors of intracellular cADPR receptors 8-Br-cADPR (80 lm) and ryanodine (50 ... activity in the bladder is associated with CD38 We first examined whether there is a difference between the degradation of nicotinamide guanine dinucleotide (NGD) to cGDPribose (cGDPR) in bladders...
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Báo cáo Y học: Dystrobrevin requires a dystrophin-binding domain to function in Caenorhabditis elegans doc
Ngày tải lên :
08/03/2014, 23:20
... on the drawing For in vitro binding experiments, the corresponding DNAs were cloned into the pGEX vector to produce Dyb-1–GST fusion proteins [18] The right column gives the binding affinity of the ... dystrophin First, we refined the dystrophin-binding region on dystrobrevin (Dyb-1) by performing deletion-mapping experiments in vitro We then tested the ability of the truncated Dyb-1 proteins to bind ... fused to the activation domain of the Gal4 yeast transcription factor and were tested against the entire C-terminal end of Dys-1 (amino acids 2857–3674) fused to the DNA-binding domain of Gal4 The...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... soon after injection of the peptide into the subphase, the lag phase for this process being too short to be measurable with our instrumentation (Fig 3C) In a typical experiment, within the first ... different initial surface pressures (p0), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the ... addition of peptide, the increase in fluorescence, owing to the binding of the dye to intracellular DNA, was measured at 37 °C in a microplate counter (Wallac 1420 Victor3Ô; Perkin Elmer, Foster City,...
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Báo cáo khoa học: Lysosomal localization of GLUT8 in the testis – the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction docx
Ngày tải lên :
16/03/2014, 02:20
... GLUT8 in the testis as well as in the brain, the tissues in which it is most abundant [23,32,35,36] In order to obtain further insights into a possible functional role of GLUT8, we attempted to ... signal to AA in the TAC chimeric protein totally abolished sorting of the chimera to an intracellular location, and led to mis-routing to the plasma membrane and ⁄ or blocking of its endocytosis ... with the late endosomal ⁄ lysosomal marker protein LAMP1, a finding that is in agreement with our in vitro binding data Targeting of GLUT8 in the absence of AP1 and AP2 In order to investigate the...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
Ngày tải lên :
16/03/2014, 02:20
... determined whether the cells were insulin unresponsive in our study at the dose and period of TNFa preincubation prior to insulin treatment used Akt is one of the most important insulin signalling intermediates, ... TNFa prior to insulin incubation As reported earlier, insulin incubation resulted in a relative increase in the nuclear exclusion of Foxa2, with it being strongly localized in the cytosol TNFa ... that the nuclear corepressor is required in the TNFa-mediated inhibition of PEPCK [54] Therefore, in cells preincubated with TNFa prior to insulin, insulin signalling is prevented, resulting in...
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Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans pot
Ngày tải lên :
16/03/2014, 03:20
... staining is present in the pharynx (arrows) and the body wall muscles (arrowheads), and a weaker signal is observed in the intestine (G) In the pharynx, a very regular expression of GSTK2::GFP in ... typical of the mitochondrial system Scale bar, 10 lm Expression was increased during morphogenesis of the embryo Fluorescence was observed as strong punctate staining in intestinal cells and in the ... of mitochondria in these two tissues, which are associated with high energy consumption In order to gain further insight into the potential function(s) of GSTK-1 and GSTK-2, RNAi was used to knock...
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Báo cáo khoa học: The astacin protein family in Caenorhabditis elegans docx
Ngày tải lên :
23/03/2014, 15:21
... in frame into a pBD95.85 vector (having the S65C mutation and artificial introns to increase the expression of GFP; A Fire Vector Kit, Baltimore, USA) according to standard protocols [23,25] The ... subgroups of the astacin protein family are indicated on the right side The schematic representation of the protein domains (colored bars) corresponds to that in Fig Meprin domains: MAM domain, MAM; ... and INTERPRO [35] protein domain databases were used For phylogenetic studies the active protease domains, covering the region from Ala-1 to Leu-200 in the prototype crayfish astacin, from the...
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Báo cáo khoa học: Identification and localization of glycine in the inner core lipopolysaccharide of Neisseria meningitidis ppt
Ngày tải lên :
23/03/2014, 21:21
... compared to the sharp singlet at 3.56 p.p.m observed for the -CH2- protons of the glycine moiety in meningococcal LPS points to an exocyclic location for the glycine residue in N meningitidis LPS The ... corresponding to glycine containing glycoforms (Table 1), thus confirming that the glycine residue is attached to the core oligosaccharide via an ester linkage In order to confirm and further characterize ... indicated in the inset figures also performed on two other meningococcal strains of clinical origin and the compositions of the glycoforms observed are listed in Table In each case where glycine...
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A pathway that links reproductive status to lifespan in Caenorhabditis elegans pptx
Ngày tải lên :
28/03/2014, 16:20
... lipase is involved in the synthesis of downstream signals from the intestine that in uence the lifespan of the other tissues in the animal The apparently conserved ability of adipose tissue to produce ... in the insulin/IGF-1 pathway, is whether loss of the germ cells activates DAF-16/FOXO by inhibiting the activity of the insulin/IGF-1 pathway Also, is the DAF-12 steroid signaling system involved? ... hatching (circles with Xs) triggers important changes in intestinal cells of the adult Killing the germ cells at the time of hatching triggers several important changes in the intestine of the...
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Báo cáo sinh học: "The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans" ppt
Ngày tải lên :
06/08/2014, 18:21
... leave the spinal cord together with the neurons of the LMC to form the ventral root Instead of growing into the developing limb, however, they leave the ventral root by a sharp dorsal turn In control ... expressed in motor neurons of both the lateral motor column (LMC) and the medial motor column (MMC) In addition to neural tissues, staining was also present in the dermamyotome (Figure 5k) Blocking the ... gray The underlined region is predicted in all cases to form a coiled-coil domain The region boxed in green is acidic, and the region boxed in red is serine/threonine-rich The bracket indicates the...
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