some biochemical parameters of embryonic and post embryonic maturation

... phosphorylation and ubiquitination, and studied the oligomerization state of the enzyme in vitro and in cells The kinetic parameters for the hydrolysis of ubiquitin substrates by full length USP7 and domain ... proteolysis of USP7 variants in the presence and absence of ubiquitin SDS ⁄ PAGE (4–20% gradient gels) showing the limited proteolysis of native USP7-FL and variants thereof by trypsin over time with and ... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal TAP-tagged...

Ngày tải lên: 07/03/2014, 05:20

... larger to smaller molecules, some of which may be highly toxic The individual products of oxidation and pyrolysis may also react and thereby produce hundreds or thousands of toxic gaseous compounds ... such as polyurethane, acrylonitrile, and nylon [5] anticoagulant for subsequent separation of serum and measurement of the required biochemical parameters Many of the above mentioned materials have ... firefighters of Jeddah and Yanbu cities by evaluation of the serum biochemical and blood hematological changes in those firefighters and compare them with normal control subjects Subjects and Methods...

Ngày tải lên: 20/06/2014, 00:20

... wastewater and reported a 95 % sCOD elimination, and a nitrogen and phosphorous removal of 50 % and 88 %, respectively These results could be generalized and the simplified automation and the possibility ... SA and ACPB are very important players in their field of activity: Unicer has the major share of the beer market in Portugal and ACPB is a very well known producer of wine with appellation of ... SA and Varnam and Sutherland, 1994) BREWERY AND WINERY A mass balance is depicted in Figure 2, which represents water and energy inputs, and also the outputs respecting residues and sub-products,...

Ngày tải lên: 22/02/2014, 05:20

... perhaps of philosophers’ ignorance of recent work in those sciences? Or is it a real and abiding feature of our understanding of the relationship between ourselves and the world of nature? And if ... science? (And, in its more pointed form, Why is contemporary philosophy of mind one of the last bastions of the philosophy of science of the 1950s?) Both this book, and several other descendents of ... However, he and I actually agree on a number of points, ranging from the failure of the classic reductionist project of Carnap and Nagel in philosophy of science to the need to adopt some form of pluralism...

Ngày tải lên: 11/06/2014, 01:40

... enhancement) while elaboration and extension account for 25.0% (18.7% of paratactic elaboration and 6.3% of hypotactic elaboration) and 29.2% (27.1% of paratactic extension and 2.1% of hypotactic extension) ... results of the study will be discussed and generalized based on the statistics to be able to contribute to improving the teaching and understanding of the conversations of this kind Design of the ... NATIONAL UNIVERSITY, HANOI UNIVERSITY OF LANGUAGES AND INTERNATIONAL STUDIES FACULTY OF POSTGRADUATE STUDIES VÕ VIỆT CƯỜNG SOME DISCOURSE FEATURES OF REQUESTS AND THEIR RESPONSES IN SHORT CONVERSATIONS...

Ngày tải lên: 28/02/2015, 11:55

... evolution of the ratio of semi-major and semi-minor radii of beads, from 18 wt% polysulfone/pyridine solution .41 Figure 5.2 Time evolution of the ratio of semi-major and semi-minor radii of ... integration of electrospinning and electrospraying has great industrial significance for production of HNIN: the fabrication of nanofibers and aggregation of inorganic nanoparticles with nanofibers ... tension of electrospinning solutions of 20 wt% with different concentration of surfactant 22 Figure 3.7 Time evolution of the diameter at the mid-point of a fluid filament of 20 wt% and...

Ngày tải lên: 26/11/2015, 12:55

... both SDS/PAGE and MALDITOF MS of the whole protein Both purified and crude samples of recombinant kynureninase can be stored in the absence or presence of PLP for extended periods of time (> 12 ... plotted using the CRICKETGRAPH and GraphPad PRISM3 software packages, and the kinetic parameters Km and Vmax were obtained using non-linear regression Lineweaver–Burk and Dixon [14] plots were used ... possibility of the existence of two isoforms of the enzyme as discussed by Toma et al [8] The pH optimum of 8.25 is consistent with previously reported experimentally determined [6] values, and the...

Ngày tải lên: 08/03/2014, 22:20

... tools, and means of transportation 3.1.2 Postharvest losses The findings of postharvest loss evaluation are presented in the following tables Postharvest losses in terms of quantity of watermelon ... 15 %, the quantities of products wasted every year are 75,505 tons of sugar cane; 46,750 tons of paddy; 3,735 tons of maize; 13,600 tons of cassava; 1,195 tons of groundnut and they are worth about ... HCN level of contents d Water-melon: analysis sugar, pH, Bx level of contents RESULTS AND DISCUSSION Postharvest losses affect quantity and quality of products Thus, the condition of the product...

Ngày tải lên: 06/08/2014, 18:22

... change in the physiology of the compatible pollen tube, shifting from an autotrophic to a heterotropic type of nutrition callose Biochemical studies focused on pollinic and stigmatic proteins, ... male-female interactions (Gaude and Dumas, 1987) After silver nitrate staining, qualitative and quantitative differences could be observed, related to the presence of P nigra and P alba pollen tubes ... Molecular and cellular events of self-incompatibility Int Rev Cytol 107,333-366 (1982) Identification of concanavalin A-binding proteins after sodium dodecyl sulfate-gel electrophoresis and protein...

Ngày tải lên: 09/08/2014, 04:20

... beginning and end of the feeding trial (Tables 3, 4, and 6) Among the biochemical parameters (Table 3), the level of protein, an indicator of adequacy of protein in terms of quality and quantity ... cava cranialis, for determination of biochemical profile and haematological and antioxidant enzyme parameters Blood samples for the determination of biochemical profiles were collected into serum ... standard deviation (mean ± SD) At the age of weeks the body weight and values of selected blood parameters (haematological, biochemical and antioxidant) were compared between the three groups of...

Ngày tải lên: 12/08/2014, 18:22

... 1981 Landry DW, Zucker HA: Embryonic death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... scientifically sound, legal, and ethical, the NIH will examine the derivation of all existing stem cell lines and create Page of (page number not for citation purposes) Theoretical Biology and Medical Modelling...

Ngày tải lên: 13/08/2014, 23:20

... transcriptomes and determine the relationship between MAPCs, MSCs and ESCs, using mouse and rat MAPCs and mouse MSCs and ESCs Results Isolation and characterization of mouse and rat MAPCs Mouse (m) MAPCs and ... ESC Figure PCA and NMF analysis of the gene-expression data of mouse MSC, MAPC-1, MAPC-2 and ESC PCA and NMF analysis of the gene-expression data of mouse MSC, MAPC-1, MAPC-2 and ESC (a) Percentage ... transcriptomes of one clone of rat MAPCs (rMAPC-1) expressing high levels of Oct4 mRNA and protein and one clone of rat cells that expressed 1,000-fold lower levels of Oct4 mRNA and differentiated...

Ngày tải lên: 14/08/2014, 08:20

... line The purpose of the study reported in this paper is to estimate and to discuss the genetic parameters of some growth and laying traits of Brown Tsaiya in Taiwan MATERIALS AND METHODS Ducks ... INTRODUCTION The estimation of genetic parameters for economically related traits has two main objectives: better understanding of the nature of genetic variation of these traits and optimization selection ... at and 16 weeks of age Egg numbers were recorded from the age at first egg up to 245, 280, and 360 days of age Egg shell thickness and strength were measured for at least eggs at 30 weeks of...

Ngày tải lên: 14/08/2014, 20:20

... narrow down the choice of candidate genes This thesis documents only the candidate genes for which I was solely responsible for the cloning of their shRNAs and measurements of OCT4 / Nanog promoter ... levels of targeted gene candidates to less than 50% in most cases, as evidenced by quantitative PCR analysis of mRNA level of candidates Mean values ± standard error of the mean (SEM) are plotted ... The measurements were done in duplicates and the average of the normalized ratio of target gene/ -actin was calculated and presented with standard error of the mean (SEM) (CAG-empty CAG vector;...

Ngày tải lên: 11/09/2015, 16:02

... identify and delineate the roles of additional factors and pathways that are important for the maintenance of ES cell self-renewal To achieve this aim, a candidate gene approach was adopted and 70 candidates ... establishment of Lefty2 as an important regulator of embryonic stem cell fate Also, this thesis extends the current understanding of ES cell self renewal mechanism beyond the established roles of LIF/STAT3, ... Nodal signals dictate embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation...

Ngày tải lên: 11/09/2015, 16:02

... 2µg and µg shRNAs were used to transfect cultures on 12 and well plate respectively A ratio of 1µg DNA: 1µl Lipofectamine2000 was used Drug selection using 1µg/ml of puromycin (Sigma) 24 hours post- transfection ... selection of 1µg/ml of puromycin and 300µg/ml of Gentamycin G418 (Gibco) selection was introduced 24 hour post transfection to select for cells transfected simultaneously with both the shRNA and the ... supplemented with 10% heatinactivated FBS and penicillin–streptomycin 4.2 RNAi design and construction of plasmids for shRNA synthesis For RNAi design and construction of plasmids for shRNA synthesis,...

Ngày tải lên: 11/09/2015, 16:02

... R., Morton, I., Moore, H D., and Andrews, P W (2004) Specific knockdown of Oct4 and beta2microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells ... regulates self-renewal of embryonic stem cells, Nat Cell Biol 10, 353-360 Jinnin, M., Ihn, H., and Tamaki, K (2006) Characterization of SIS3, a novel specific inhibitor of Smad3, and its effect on ... C., and Hayek, A (2005) Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and...

Ngày tải lên: 11/09/2015, 16:02

... die of cardiac defects and display right pulmonary isomerism (Oh and Li, 1997) Right pulmonary isomerism and randomized embryonic turning was also observed for Cryptic mutant (co-receptor of Nodal) ... into its extraembryonic and embryonic components The outer layer of cells of the blastocyst is the extraembryonic components and forms an epithelium, the trophectoderm Descendents of the trophectoderm ... process per se Klf4 and c-Myc have more to with the regulation of the growth and self renewal of iPS cells rather than the induction of pluripotency per se, as overexpression of Klf4 and c-Myc resulted...

Ngày tải lên: 11/09/2015, 16:02

... co-localisation of Oct4-Sox2, Stat3 and Smad1 on the Mycn and Sgk loci 7.6 Co-localisation of Stat3 and Smad1 on Oct4 and Sox2 overlapping binding sites 7.7 Co-localisation of Oct4, Sox2, Stat3 and Smad1 ... differentiation of mESCs reduces Oct4 and Sox2 levels and binding, and abolishes Stat3 and Smad1 binding 7.10 Knockdown of Oct4 and Sox2 in mESCs differentiates the cells, significantly reduces Oct4 and ... hindbrain region and posterior of the embryo (Chung and Cooney, 2001; Fuhrmann et al., 2001) This indicates that loss of Gcnf leads to loss of Oct4 repression in somatic cells and loss of Gcnf-induced...

Ngày tải lên: 11/09/2015, 16:03