snake venom peptides promising molecules with anti tumor effects

... photodynamic activity The group treated with the drug without light exposure showed that the drug alone had no effects on tumor cells which coincided with the result of biosafety assay In this ... In Vitro Studies with Tumor Cells Preparation of PHPP-SMNPs Solution PHPP-SMNPs was diluted to 100 lmol L-1 with 0.5% carboxymethylcellulose sodium The solution was then diluted with RPMI-1640 ... HCl Therefore, the 409 nm was the characteristic absorption peaks of PHPP with or without SMNPs The PHPP-SMNPs treated with HCl were detected by UV–Vis spectrophotometer and the spectrums are...

Ngày tải lên: 22/06/2014, 01:20

... were then inoculated with × 105 CT26 tumor cells Frozen sections of tumor tissue were tested by immunohistochemical analysis with anti- CD31 antibody (A-D) Vessel density in tumor tissues from human ... Laboratory for Biotherapy) Therapeutic anti- tumor immunity and tumor models Experiments with CT26 and 4T1 tumors were carried out in female BALB/c mice, 6–8 weeks of age Tumors were established by sub-cutaneous ... death of mice, the tumor volumes on day 28 (CT26 tumors) and day 34 (4T1 tumors) in the control groups only came from the surviving mice Protective anti- tumor immunity and tumor models At 6–8...

Ngày tải lên: 14/08/2014, 19:22

... studies in patients of European ancestry showing that anti- CCP2 and anti- CCP3 antibodies exhibit high diagnostic specificity for RA with anti- CCP2 antibodies having the highest PPV and NPV However, ... F: Do all anti- citrullinated protein/peptide antibody tests measure the same? Evaluation of discrepancy between anti- citrullinated protein/peptide antibody tests in patients with and without rheumatoid ... [12] Anti- cyclic Citrullinated Peptide antibody determination by enzyme immunoassay The ELISA kits detecting the IgG anti- CCP2 antibodies (Immunoscan RA: regular, second generation of CCP antigen),...

Ngày tải lên: 12/08/2014, 11:23

... patients with AS completed the 12week treatment with adalimumab During the complete study, 1% of patients with AS (10 of 924) without prior anti- TNF therapy and 3% of patients with AS (11 of 326) with ... NSAIDs, nonsteroidal antiinflammatory drugs; TNF, tumor necrosis factor with a history of anti- TNF therapy BASDAI 50 responses were achieved by 63.0% of patients with AS without prior anti- TNF therapy ... of 376) without prior anti- TNF therapy and 3% of patients (two of 66) with prior antiTNF therapy withdrew from the study because of AEs For both studies, the mean/median treatment period with adalimumab...

Ngày tải lên: 12/08/2014, 14:22

... tumors, emphasizing the importance of overcoming pRB-mediated growth control for tumor progression Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 233 Two classes of human tumors ... exit with the induction of differentiation that confers upon pRB its tumor suppressor function Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 237 The interaction of pRB with ... et al 2003) tumorigenesis, but worsened thyroid tumors Decreased neuroendocrine (Lee et al 2002) tumorigenesis and increased survival Increased neuroendocrine (Brugarolas et al 1998) tumorigenesis,...

Ngày tải lên: 25/10/2013, 21:20

... expression of ENOA increased with tumor de-differentiation and correlated positively with venous invasion [93,94] In breast and lung cancer patients, anti- ENOA autoantibodies are decreased in ... analysis of autoantibodies in sera of patients with cancer Rinsho Byori 53, 437–445 129 Dot C, Guigay J & Adamus G (2005) Anti- alpha-enolase antibodies in cancer-associated retinopathy with small ... occurs at an early stage of tumorigenesis, as illustrated by the detection of high titers of autoantibodies in patients with early-stage cancer [64], and correlates with the progression of malignant...

Ngày tải lên: 14/02/2014, 19:20

... viperid snake envenomation and novel toxin-based drug discovery Venom protein biosynthesis Snake venoms are the products of specialized secretory glands located above the upper jawbone in venomous snakes ... investigations of snake venom and venom glands respectively, numerous databases have been generated that illuminate the complexity of snake venoms, particularly the viperid venoms Hundreds of ... S M T Serrano Snake venom metalloproteinases and venom complexity Fig Continued FEBS Journal 275 (2008) 3016–3030 ª 2008 The Authors Journal compilation ª 2008 FEBS 3021 Snake venom metalloproteinases...

Ngày tải lên: 18/02/2014, 18:20

... thrombin-like snake venom enzyme J Biol Chem 262, 3132–3135 163 Stocker K (1977) Defibrinogenation with thrombin-like snake venom enzymes In Handbook of Experimental Enzymatic toxins from snake venom 164 ... Elapidae, with the exception of Dendroaspis species [12] In contrast, AChE is not found in venoms of snakes belonging to the Viperidae and Crotalidae families [3,13] Incidentally, snake venom AChEs ... and other Elapidae venom exists as soluble monomers that are not associated with either anchoring proteins or cell membranes [15] Sequence comparisons of snake venom AChE with other AChEs demonstrate...

Ngày tải lên: 14/03/2014, 22:20

... that HLTX-like proteins would be widespread in snake venoms, we generated an rabbit anti- tigrin serum We then screened several snake venoms with the anti- tigrin serum using Western blotting or ELISAs ... proteins homologous to ablomin are found in several snake venoms and represent new snake venom proteins with neurotoxin-like activities DISCUSSION Snake venom neurotoxins, represented by a-neurotoxins ... procedure, we isolated a novel snake venom protein (named ablomin) from the venom of the Mamushi snake (A blomhoffi) through three purification steps First, the crude venom of A blomhoffi was separated...

Ngày tải lên: 18/03/2014, 01:20

... mechanisms may lead to new therapeutic strategies to sensitize endothelial cells to anti- angiogenic and conventional anti- tumor treatments In this study, we therefore exposed endothelial cells to cyclic ... Akt and eNOS activation have opposite effects on HIF-1a during cyclic hypoxia; caution is therefore required in the use of NOS inhibitors as single anti- tumor treatments More generally, by providing ... inhibitor The origins of cyclic exposure of cells within tumors to various pO2 levels are multiple as described above Here we focused on the effects of CyH on endothelial cells, a cell type that...

Ngày tải lên: 30/03/2014, 02:20

... the dark with the following monoclonal antibodies (mAbs): anti- fibroblast (ASO2-PE, Dianova, Hamburg, Germany), anti- MHC class I (W6/32-FITC, IgG2a; Cymbus Biotechnology, Eastleigh, UK), anti HLA-E ... urgent need to treat patients with Hsp70 membrane-positive tumors A phase I clinical study involving eleven patients with metastatic colorectal cancer and one patient with non-small cell lung cancer ... incubated with the following mAbs as described above: anti- CD3 and anti- CD16/56-tricolor-conjugated http://www.translational-medicine.com/content/7/1/50 (Caltag, Hamburg, Germany), anti- CD94-FITC...

Ngày tải lên: 18/06/2014, 15:20

... inoculated s.c on right hind leg with × 105 MC-38 or B16 tumor cells and treated with different HIFU on day of tumor inoculation Mice were challenged with × 106 MC-38 or B16 tumor cells by s.c inoculation ... can reduce the primary tumor mass and elicit simultaneously a strong anti- tumor immune response are highly desirable The induction and maintenance of an effective antitumor immune response is ... thermal lesion Tumor tissue samples were collected day after HIFU treatment 6μm cryostat sections were cut and stained with anti- CD11c Abs Then the antibody was visualized using the Anti- Hamster...

Ngày tải lên: 18/06/2014, 16:20

... incubated with blocking solution containing 3% normal goat serum, 3% bovine serum albumin, and 0.4% Triton-X 100 in 0.15 M PB for hr Slides were incubated with rat monoclonal anti- F4/80 antibody ... times for each with 0.15 M PB prior to incubation with Vectastain biotinylated goat anti- immunoglobulin (Vector Laboratories, Burlingame, CA) for hrs at RT Excessive secondary antibody was washed ... activity, sections were incubated with 3% H2O2 and 3% methanol for 25 Sections were then washed twice for each with PBS, followed by epitope retrieval treatment with 90% formic acid for at RT Next,...

Ngày tải lên: 19/06/2014, 22:20

... promoting tumor survival In contrast, ovarian tumors can also elicit a marked host immune response resulting in the influx of tumor infiltrating lymphocytes into the tumor which recognize antigens ... growth as well as tumorigenesis In addition to the role of the tumor microenvironment, alterations in apoptotic regulation promoting an antiapoptotic phenotype also support tumor progression ... CyAd PD Figure Extent of epithelial Bcl-2 staining decreases with tumor progression Extent of epithelial Bcl-2 staining decreases with tumor progression Extent of epithelial Bcl-2 staining was observed...

Ngày tải lên: 20/06/2014, 07:20

... more sensitive imaging of the biomarkers with SPECT Keywords: 111In-radiolabelling; peptides; antibodies; chelator Introduction Radiolabelled peptides and antibodies are used for molecular imaging ... for DTPA-octreotide and DOTA -anti- CAIX antibody were five times higher in MES and HEPES buffer Radiolabelling of DTPA-exendin-3, DOTA-exendin-3 and DTPA -anti- CAIX antibody in MES and HEPES buffer ... two peptides, exendin-3 and octreotide, and the chimeric monoclonal antibody [mAb] targeting carbonic anhydrase IX [CAIX], each conjugated with DTPA or DOTA, were used Experimental procedures Peptides...

Ngày tải lên: 20/06/2014, 20:20

... more sensitive imaging of the biomarkers with SPECT Keywords: 111In-radiolabelling; peptides; antibodies; chelator Introduction Radiolabelled peptides and antibodies are used for molecular imaging ... for DTPA-octreotide and DOTA -anti- CAIX antibody were five times higher in MES and HEPES buffer Radiolabelling of DTPA-exendin-3, DOTA-exendin-3 and DTPA -anti- CAIX antibody in MES and HEPES buffer ... two peptides, exendin-3 and octreotide, and the chimeric monoclonal antibody [mAb] targeting carbonic anhydrase IX [CAIX], each conjugated with DTPA or DOTA, were used Experimental procedures Peptides...

Ngày tải lên: 21/06/2014, 17:20

... primary tumors and the extension of BTT Tumor size was measured directly in the surgical specimen by pathology examination A tumor satellite is defined as any daughter tumor < cm in size lying within ... indicated that the HCC patients with BTT had smaller tumors and a higher percentage of a tumor size ≤ cm than those without BTT [6] Similarly, in this study, the incidence of tumor size ≤ cm tended to ... from the tumor may partially or completely fill the biliary tree with blood clots [18] Generally, the tumor thrombus was not adherent to the bile duct wall so it could be removed easily Tumor thrombi...

Ngày tải lên: 09/08/2014, 01:24

... (71) (86) (29) (14) ANA, antinuclear antibodies; anti- CCP, antibodies against cyclic citrullinated peptides; anti- dsDNA, antibodies against double-stranded DNA; anti- RNP, antibodies against ribonucleoprotein; ... anti- dsDNA antibodies, RF and anti- extractable nuclear antigen antibodies, Page of (page number not for citation purposes) Analysis of serum anti- CCP antibodies Patients with rhupus and those antibodies ... rhupus and those antibodies with rheumatoid arthritis (RA) had significantly higher serum antibodies against cyclic citrullinated peptides (anti- CCP antibodies) than patients with systemic lupus erythematosus...

Ngày tải lên: 09/08/2014, 08:22

... Patients with anti- CCP antibodies (n = 318) had an earlier onset of the disease compared with patients without these autoantibodies (n = 150) (53.2 years for patients with anti- CCP antibodies ... only in anti- CCP antibody-positive patients with RA [17] Considering our findings of a stronger predictive value of the combination of PTPN22 1858T variant with anti- CCP antibodies compared with ... for patients without anti- CCP antibodies, P < 0.01) A combination of the 1858T variant and anti- CCP antibodies contributed to an even earlier age of disease onset (1858T + anti- CCP antibodies,...

Ngày tải lên: 09/08/2014, 10:20

Ngày tải lên: 09/08/2014, 10:21