smooth muscle cell activation and synthesis of extracellular matrix

... Campbell, J.H (1986) Replication of smooth muscle cells in vascular disease Circ Res 58, 427–444 Owens, G.K (1995) Regulation of differentiation of vascular smooth muscle cells Physiol Rev 75, 487–517 ... proliferation of human and rat vascular smooth- muscle cells Biochem J 283, 403–408 Saward, L & Zahradka, P (1997) Coronary artery smooth muscle in culture: migration of heterogeneous cell populations ... both the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed...

Ngày tải lên: 17/03/2014, 09:20

... proliferation of rat and bovine PASMC, airway -smooth muscle cells, lung fibroblasts and mesangial cells [30-33] Our findings that DNA synthesis was increased during moderate hypoxia, and that the HPASMC cell ... effect of PDGF on p27kip1 expression has been demonstrated using rat aortic vascular smooth muscle cells [37] and in human saphenous vein smooth muscle cells [38], and oncogenic Ras induces cell ... inhibition of CDK activity and in the proliferation of vascular smooth muscle cells [8,21-23] On the other hand, Li et al found that BPS suppresses systemic vascular smooth muscle proliferation through...

Ngày tải lên: 12/08/2014, 15:21

... the cells to secrete components of the extracellular matrix [25,26] Thus, reducing the number of ASMC in asthma may resolve several pathologies and therefore improve lung function and quality of ... role of JNK, p38, and ERK1/2 MAPK activation and GSH in DMF induced HO-1 expression Methods Isolation, characterisation and culture of human ASMC Human ASMC were isolated and grown from bronchi of ... contribution of ASMC to asthma comes from a novel form of therapy: thermoplasty of ASMC This therapy eliminated ASMC by over heating the cells by means of radio-waves and results in lasting improvement of...

Ngày tải lên: 12/08/2014, 11:22

... cells to decrease levels of smooth muscle contractile proteins like sm-α-actin, calponin, and smooth muscle myosin heavy chain, and to increase synthesis of collagen and other extracellular matrix ... regulates migration of smooth muscle cells Nucleofection was used to introduce DAPK cDNA into the primary human coronary artery smooth muscle cells (HCASMC), and then the ability of these cells to migrate ... muscle cells, and cell adhesion and migration are important roles of smooth muscle cells in the genesis of vascular disease, the role of DAPK in vascular smooth muscle cells has not been well studied...

Ngày tải lên: 24/08/2014, 11:25

... (excitation and emission filters of 530 and 616 nm, respectively) A standard cell suspension of every cell type was prepared in DPBS and kept at -80°C until use The growth fraction of exponentially ... Characterization of the cell types and growth kinetics of cultured cells Healthy growing EC and SMC cultures were obtained from UC of group-1 and group-2 individuals No contamination of SMCs in EC ... weight and growth characteristics of cultured cells No differences in terms of average time to reach passage cell density, percentage of viable cells and senescence level were found for each cell...

Ngày tải lên: 18/06/2014, 15:20

... can increase the smooth muscle cell volume and change the contractile properties of smooth muscle cells and airway reactivity [22-24], as well as to promote ASMC proliferation and participate ... removed, the cells on the upper side were scraped off, and the cells that migrated to the lower side of the membrane were fixed with 4% polyoxymethylene The number of cells was counted in random fields ... characteristic of asthma The airway pathological features of asthma include reshaping of smooth muscle cell proliferation, hypertrophy, airway epithelium metaplasia, fibrosis, Li et al Journal of Inflammation...

Ngày tải lên: 11/08/2014, 03:20

... can increase the smooth muscle cell volume and change the contractile properties of smooth muscle cells and airway reactivity [22-24], as well as to promote ASMC proliferation and participate ... removed, the cells on the upper side were scraped off, and the cells that migrated to the lower side of the membrane were fixed with 4% polyoxymethylene The number of cells was counted in random fields ... characteristic of asthma The airway pathological features of asthma include reshaping of smooth muscle cell proliferation, hypertrophy, airway epithelium metaplasia, fibrosis, Li et al Journal of Inflammation...

Ngày tải lên: 11/08/2014, 06:23

... searches as target antigens of antibodies to proteins in the proteome of VSMCs and HUVECs Vinculin is a cytoskeleton protein involved in extracellular matrix adhesion and intercellular junctions by ... article as: Rộgent et al.: Identification of target antigens of anti-endothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach Arthritis ... lamin in serum of giant cell arteritis patients Protein extract is from vascular smooth muscle cells (VSMCs) (A) IgG reactivity to lamin in five different pools of sera from three giant cell arteritis...

Ngày tải lên: 12/08/2014, 17:22

... analyses of infected A549 cells (Fig 6a and 6b) 40 Figure growth of L pneumophila in A549 cells Intracellular Intracellular growth of L pneumophila in A549 cells A549 cells were infected with ... examined the influence of methyl prednisolone, as an inhibitor of cell injury, on DNA fragmentation, caspase activation, and secretion of HMGB1 from L pneumophila-infected A549 cells Cell culture The ... cytotoxicity of L pneumophila in A549 cells First, we verified the infection and intracellular growth of L pneumophila in A549 cells Intracellular growth of the virulent strain was observed day...

Ngày tải lên: 12/08/2014, 15:21

... non-stimulated PBMCs Cell proliferation and activation of ERK1/2 are important for induction of cellular responses such as the expression of cellular activation markers CD25 or secretion of IL-2 In infected ... for both CD3+ T cell activation as well as induction of IL-2 and IL-6 secretion in vivo The activation status of Tcells in the GALT and the ability of the virus to induce secretion of these cytokines ... http://www.retrovirology.com/content/8/1/14 Page 11 of 17 Figure Kinetics of plasma cytokine levels and activation markers on T cells of infected macaques in vivo (A and B) Serum levels of (A) IL-2 and (B) IL-6 in blood samples...

Ngày tải lên: 13/08/2014, 01:20

... Examples of smooth muscle- specific proteins include smooth muscle α and γ actin, SM-MHC, caldesmon, SM22α, telokin and calponin Although all of these proteins are expressed in smooth muscle some of ... Structure and functions of the colon Regulation of colonic contractility .2 Differentiation and development of the colon smooth muscle Smooth muscle contractile and regulatory ... first layer of smooth muscle myoblasts differentiate and give rise to the inner circular smooth muscle layer Around embryonic day 12 primitive crypts are formed and smooth muscle cells in the...

Ngày tải lên: 24/08/2014, 12:32

... CHARACTERIZATION OF BIOPOLYMER AND THE EFFECTS OF MECHANICAL PROPERTIES OF EXTRACELLULAR MATRIX ON CELL BEHAVIORS WONG LONG HUI (B.Eng.(Hons.), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... engineering, smooth muscle cells and other tissue cells exhibit lower ECM synthesis when cultured in collagen compared to fibrin The synthesis of additional ECM is required to increase the stiffness and ... regulation of this tension: intracellular and extracellular Intracellular forces refer to the exertion of forces by structural protein networks inside the cells while extracellular forces refer to the...

Ngày tải lên: 09/09/2015, 11:28

... analysis and chemical synthesis of these peptides muscle- acting a-conotoxin MI and the neuronally acting a-conotoxin MII [11–13] A biological interpretation of this emerging pattern of paired ligand ... [1], and it seems likely that the complement of neuronally acting a-conotoxins in one species may cover a range of subtype specificities There are examples of combinations of muscle- type and neuronaltype ... subtypes, size, route of discovery, method of synthesis and reference for synthesis Asterisks (*) indicate an amidated C-terminus The letters O and Y denote hydroxyproline and sulfotyrosine, respectively...

Ngày tải lên: 19/02/2014, 12:20

... functions This mechanism leads to activation of phenoloxidase, which oxidizes catechols, leading to the formation of toxic quinones and melanin [1,2], and to the activation of cytokines that stimulate ... SPE and Easter [31] Injection of either of these M sexta proteinases into larvae stimulated the expression of antimicrobial peptide genes, suggesting that they might function in activation of ... longer form Ten splicing isoforms of Drosophila Spat¨ zle occur in the precellular blastoderm embryo [8] One pair of splicing isoforms appears as D melanogaster Spatzle 11.7 and 11.15, with amino...

Ngày tải lên: 06/03/2014, 09:22

... controls The subcellular distribution of PLD and the consequences of priming and activation were determined using immunoprecipitation and Western blot analysis of neutrophil cytosol and membrane ... neutrophils showing that activation of O2 À generation occurs in parallel with the elevation of both PtdOH and DAG levels [40] and in experiments using cell- free systems where PtdOH and DAG work collectively ... engulfment of nonopsonized zymosan particles Others have shown that an epitope-tagged form of PLD1 expressed in RBL-2H3 cells localizes to secretory granules and lysosomes of unstimulated cells and, ...

Ngày tải lên: 07/03/2014, 15:20

... well as synthesis of the distribution of the consonants across the languages In the current work, with the help of PlaNet we provide a systematic study of certain interesting features of the consonant ... set of nodes labeled by the languages and VC is the set of nodes labeled by the consonants E is the set of edges that run between VL and VC There is an edge e ∈ E between two nodes vl ∈ VL and ... the other hand Regime of Figure 3(a) as well as Regime of Figure 3(b) comprises of the rest of the consonants The point marked as x in both the figures indicates the breakpoint Each of the regime...

Ngày tải lên: 08/03/2014, 02:21

... side is shown the position of the 225 and 92 kDa forms of proMMP-9 in serum-free culture medium of THP-1 cells and the 72 kDa form of proMMP-2 in serum-free culture medium of human skin fibroblasts ... right is shown the position of the 225 and 92 kDa forms of proMMP-9 in serum-free culture medium of THP-1 cells and the 72 kDa form of proMMP-2 in serum-free culture medium of human skin fibroblasts ... the stacking and the separating gel At the left side is shown the position of the 225 and 92 kDa forms of proMMP-9 in serum-free culture medium of THP-1 cells and the 72 kDa form of proMMP-2 in...

Ngày tải lên: 17/03/2014, 10:20

... concentrated, and lyophilized to produce (5.5 mg) and (7.2 mg) in 5.2 and 6.8% yields based on the acceptor added, respectively 1H- and 13C-NMR data of and are summarized in Table Preparation of GlcNAcb1-3Galb1-4GlcNAcb-pNP ... analysis of the reaction mixture obtained by endo-bgalactosidase-mediated transglycosylation and time courses of the production of transglycosylation products and 10 from and degradation of (A) ... Galb14GlcNAcb1-3Galb and GlcNAcb-pNP The relative hydrolytic rates of and compared with that of (set at 100) were 47 and 10, i.e 2- and 10-fold differences, respectively The hydrolytic activities toward 3, and...

Ngày tải lên: 31/03/2014, 07:20

... MOVCAR-10 cells were fixed and expression of intracellular Muc16 was detected by using the 618F antibody (grey line) Dark line shows isotype control (C) Expression of cell surface and intracellular ... Specificity of 618F for human MUC16 Specificity of 618F for human MUC16 (A) Purity of human MUC16 isolated from spent media of OVCAR-3 cells as determined by Coomassie Blue (1) and silver staining (2) of ... 618F % of Max % of Max GAM-FITC GAM-FITC C 180 c Extracellular GAM-FITC D d 160 140 120 100 80 60 40 Mean Fluorescence Intensity 60 Intracellular Mean Fluorescence Intensity 65 55 Extracellular...

Ngày tải lên: 20/06/2014, 07:20

... variations are OK ECE574: VHDL and Verilog Modeling and Synthesis 12/08/10 ECE574: VHDL and Verilog Modeling and Synthesis 12/08/10 Part 2: Open Book (Text book and Notes allowed) NAME: ... count from to 77 on a negative edge of the clock and then restart at ECE574: VHDL and Verilog Modeling and Synthesis 12/08/10 Question [20 marks]: An FPGA running off its own local 100MHz oscillator ... Applications of LFSRs include generating pseudo-random numbers, pseudo-noise sequences, fast digital counters, and whitening sequences Both hardware and software implementations of LFSRs are...

Ngày tải lên: 11/07/2014, 02:21