smoking anxiety and nicotinic acetylcholinergic receptors
báo cáo khoa học: " The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro" ppsx
Ngày tải lên :
11/08/2014, 20:20
... primer and Real-Time PCR, the efficiency was calculated on the basis of the SYBR Green fluorescence curves and the standard dilution series The relative gene expression levels were standardized ... of zoledronate, ibandronate and clodronate on cyclin D1 gene expression As shown in figure 1, treatment of human hip bone osteoblast [hOB] cells with ibandronate, zoledronate and clodronate did ... consulting and coordination and helped to draft the manuscript All authors read and approved the final manuscript Acknowledgements Thanks to the laboratory staff Author Details 1Department of Oral and...
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DEVELOPMENTAL DIFFERENCES AND ALTERED GENE EXPRESSION IN THE TS65DN MOUSE MODEL OF DOWN SYNDROME
Ngày tải lên :
24/08/2014, 12:33
... Dlx3, Dlx5, and Dlx5 are expressed in the mandibular processes, and Dlx5 and Dlx6 are also expressed in developing bones, cartilage, and teeth and may play an important role in mandibular formation ... Meckel’s and the hyoid cartilage which would further delay mandibular growth 17 CHAPTER MATERIALS AND EXPERIMENTAL METHODS 2.1 Ts65Dn and B6C3F₁ Breeding Female B6EiC3Sn a/A-Ts(1716)65Dn (Ts65Dn) and ... bacteriolytic function and no known effect on mandibular development To try and focus on genes that would have a direct effect on mandibular growth, genes that were involved in cartilage and skeletal development...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... progression and maintenance of the cell cycle As MLLs and H3K4-specific methylations are well known to play critical roles in gene expression, we analyzed the dynamics and functions of MLLs and H3K4 ... of RNAP II and MLL1 recruitment and H3K4 trimethylation marks were observed at the beginning of the S phase (0 h), and these marks were attenuated for the rest of the cell cycle (10 and 20 h), ... regulation of HoxA5, HoxA7 and HoxA10 genes and cell cycle progression, we knocked down MLL1 using a specific antisense oligonucleotide and analyzed the expression of Hox genes and cyclins As shown...
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báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf
Ngày tải lên :
10/08/2014, 10:21
... at 59°C for 35 s and extension at 72°C for min, and followed by the final extension at 72°C for 10 The primers were shown in Table and Table Materials and methods Cell culture and transfection ... demethylation Hela and Siha cells were transfected with siRNA and treated with 1.0 μM 5az-dC (Sigma) respectively, and harvested at 72 h after incubation Genomic DNA was extracted and randomly sheared ... SFRP4, TSLC1 and CHFR in Hela cells and CCNA1, PTEN, SFRP4 and TSLC1 in Siha cells were analyzed Surprisingly, transfection did not affect the methylation status and mRNA expression of FHIT and PTEN...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
Ngày tải lên :
12/08/2014, 02:20
... assays and IMTC participated in virus culture and virus isolation WYL was responsible for experimental design, analyses and drafting of the manuscript PKSC was responsible for design and supervision ... on macrophages and monocytes infected in vitro or in vivo [35-37] The mechanism concerning bronchial infiltration of inflammatory cells, particularly lymphocytes and eosinophils, and the subsequent ... inflammatory response and host resistance to pathogens [34,41] TNF-a may activate nuclear factor-kB (NF-kB) by inducing the phosphorylation and degradation of inhibitory factor-kB (IkB) and leads to the...
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Báo cáo y học: "Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes" docx
Ngày tải lên :
13/08/2014, 14:20
... 2), AC (6.25, 12.5 and 25 μM; Lanes to 5), antiCD3/CD28 Ab (Lane 6), DMSO and anti-CD3/CD28 Ab (Lane 7), and AC and anti-CD3/CD28 Ab (Lanes to 10) respectively (Figure 4A and 4B) The results ... AC and anti-CD3/CD28 Ab (C) and (D): Lane - 0.1% DMSO, Lane - CsA, Lane - DMSO and anti-CD3/CD28 Ab, Lane - CsA and anti-CD3/ CD28 Ab Graphical representation of laser densitometry of IL-2 and ... 0.001) and IFN-g (P = 0.008) expression in the cells (Figures 4C and 4D) CsA also significantly reduced IL-2 (P = 0.001) and IFN-g (P = 0.003) production in the activated cells (Figures 3A and 3B)...
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Báo cáo y học: " Gene therapy in animal models of rheumatoid arthritis: are we ready for the patients" pdf
Ngày tải lên :
09/08/2014, 01:24
... II membrane proteins and activate death receptors for transduction of apoptotic signals Activated lymphocytes and synoviocytes from RA patients express TRAIL receptors, and adenoviral TRAIL gene ... strategy to protect cartilage and chondrocytes in arthritis given in this illustration are reviewed in detail by van der Kraan and coworkers [111] and by Evans and coworkers [112] arthritic animal ... ligand (FasL; see Genetic synovectomy, below) [25,26] The DCs not migrate to the joint, and exert their effects in the spleen and lymph nodes The most sophisticated approach is that from Zhang and...
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Curcumin modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats potx
Ngày tải lên :
10/08/2014, 05:21
... 12 hour light and 12 hour dark periods Rats had free access to standard food and water ad libitum All animal care and procedures were done in accordance with the Institutional and National Institute ... forward primer, reverse primer and TaqMan probe for assay on demand and endogenous control β-actin and 12.5 μl of Taqman 2× Universal PCR Master Mix (Applied Biosystems) and the volume was made up ... dopaminergic receptors, CREB and phospholipase C in the cerebral cortex and cerebellum of STZ-induced diabetic rats Our present study on curcumin dependent regulation of dopaminergic receptors, ...
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Báo cáo khoa học: "Changes in orexin-A and neuropeptide Y expression in the hypothalamus of the fasted and high-fat diet fed rats" doc
Ngày tải lên :
07/08/2014, 18:20
... 67.9 ± 0.9 and 88.9 ± 0.6 in 24 h and 84 h of fastings, respectively (Figs 8A, B and 9) In the SCN, NPY immunoreactivity per unit area (0.01 mm2) was 77.8 ± 3.8 and 88.9 ± 2.6 in 24 h and 84 h ... (Figs 8C, D and 10) Expression of OXA- and NPY- immunoreactivities in the high-fat and normal diet fed groups In the HF and NF diet fed groups, the OXA-IR neurons were observed in the LHA, and they ... immunoreactivity of the ARC and the SCN between the HF and NF diet fed groups (p < 0.01, Figs 14 and 15) Discussion The present study was aimed to understand the changes of Changes in orexin-A and neuropeptide...
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Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx
Ngày tải lên :
09/08/2014, 10:21
... (CCR)7 and its ligand CC chemokine ligand (CCL)19 The ligands CCL19 and CCL21, and their receptor CCR7 have been found to be involved in homing of lymphocytes to the target organ [37] and to ... congenic mice Red and blue genes have FC above and FC below -2, respectively Orange and green have FC from 1.5 to and -2 to -1.5, respectively Yellow and light blue are upregulated and downregulated, ... expressed, and in B10.Q.Nss1 13 chemokines and eight cytokines were upregulated Several chemokines, among these lymphotoxin β (Ltb), Cxcl13, Cxcl16 and Ccl19, and the cytokines Socs1 and Socs3...
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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
Ngày tải lên :
09/08/2014, 14:20
... Histological studies by Bachmeier and colleagues [10,11] have shown TNF-α and its receptors to be expressed in normal IVD and upregulated with age and degeneration Seguin and colleagues [12] have demonstrated ... aggrecan and type II collagen genes and upregulates mRNA expression of matrix metalloproteinase (MMP)-1, -3 and -13 and ADAM-TS4 (a disintegrin and metalloproteinase with thrombospondin motif 4) and ... regulation and degradation in normal and degenerate NP cells isolated from human adult tissue and investigates how TNF-α and IL-1β may be involved in regulation of themselves and each other and their...
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Báo cáo y học: "Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver" ppt
Ngày tải lên :
11/08/2014, 08:21
... DMSO and LPS (1 mg/kg, i.p.) or 100 µl water Spleen and liver were collected after 90 minutes and total RNA was extracted and subjected to real-time, one-step RT-PCR using TaqMan primers and probes ... levels in spleen and liver following IL-1β mRNA and LPS co-treatment IL-1β mRNA expression levels in spleen and liver following parthenolide and LPS co-treatment Mice were treated and analyzed for ... parthenolide and LPS co-treatment spleen and liver following COX-2 COX-2 mRNA expression levels in spleen and liver following parthenolide and LPS co-treatment Mice were treated and COX-2 mRNAs...
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Báo cáo y học: "Response of the mouse lung transcriptome to welding fume: effects of stainless and mild steel fumes on lung gene expression in A/J and C57BL/6J mice" pot
Ngày tải lên :
12/08/2014, 11:22
... GMA-MS and GMA-SS exposure Reported in tables 1, 2, and are the associated categories of diseases and disorders, molecular and cellular functions and physiological system development and functions ... connective tissue disorders and skeletal and muscular disorder were significant in both A/J and B6 mice (Table and 4) However, as previ- ously mentioned, the gene networks, numbers and regulation contrasted ... arrays and the IPA analysis and drafted the manuscript MLK and SL were responsible for the statistical design, data management, statistical analysis and clustering analysis for these studies JMA and...
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Báo cáo y học: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals" ppt
Ngày tải lên :
12/08/2014, 23:22
... Env-Ory1- and Env-Ory2, and of the human and mouse syncytins The tree was determined by the neighbor-joining method using envelope TM subunit sequences (see ref [12]) from murine and human ERVs, and ... observed for retroviruses Their position and functionality ¢ Figure the the human identified syncytins tions of and mouserabbit Env-Ory1- and Env-Ory2, and of Retroviral envelope protein-based ... products obtained from Lepus and Sylvilagus were cloned and sequenced and showed >88% nucleotide identity with the Oryctolagus cuniculus syncytin-Ory1 gene sequence, and therefore most probably...
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Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
Ngày tải lên :
13/08/2014, 23:20
... content, which goes down to 2N and back to 4N and never repeats the 8N In between the start and the end there is no systematic variation of cells through G1, S and G2 In summary, the work of ... object model) standards, so that the public data can be analyzed 18) Microarray results should be compared to randomized data to show that the observed cyclicities are not the result of random noise ... cell cycle, and during the period of cell division there should be a bimodal distribution of cell sizes One should apply clearly stated and stringent criteria for synchronization and microarray...
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Báo cáo y học: "Genome-wide patterns of carbon and nitrogen regulation of gene expression validate the combined carbon and nitrogen (CN)-signaling hypothesis in plants" pptx
Ngày tải lên :
14/08/2014, 14:21
... regulator of carbon and nitrogen metabolism in A thaliana [19], but a global understanding of the genes and processes that are regulated by carbon and nitrogen signaling in plants and the mechanism ... total of four copies of CN1 and CN2 was very unlikely (p-value = 0.018) in any combination (for example, three CN1 and one CN2, two CN1 and two CN2, or one CN1 and two CN2, and so on) A second mechanism ... ferredoxin reductase and ferredoxin-dependent nitrite reductase are co-regulated by carbon and nitrogen due to CN1 and/ or CN2 CN1 and/ or CN2 therefore might act to link nitrogen reduction and energy metabolism...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
Ngày tải lên :
02/11/2012, 11:08
... normal human T-lymphocytes and the Jurkat T-cell line Expression of set, SMG-1, and ND4 was unchanged, and pub and a-myb were not transcribed in normal human T-lymphocytes and the Jurkat cells (data ... HIV-associated and monkey SIV-associated lymphomas and seemed to be common for virus-specific lymphomagenesis in human and monkey Table Selected human HIV-associated lymphomas h1 and h2 genes overexpressed ... presented in Fig 2a, 2b and Table Figure Northern blot analysis of the transcription levels of the set, ND4, and SMG-1 genes in human spontaneous lymphomas and normal B- and Tlymphocytes (a) 32P-labeled...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
Ngày tải lên :
03/11/2012, 11:44
... Material and Methods Peptide Synthesis and Purification For solid phase synthesis of the peptides RQIKIWFQNRRMKWKK [pAnt43-58] and YGRKKRRQRRR [HIV-1 Tat48-60] and KKMTRQTWWHRIKHKC [TP6288] and 6287] ... chain protecting groups were employed: Boc(tert-butyloxycarbonyl) for Lys and Trp, Trityl(triphenylmethane) for Gln and Asn and Pbf (2,2,4,6,7-Pentamethyldihydrobenzofurane-5-sulfonyl ) for Arg Fmoc-Lys(Dabcyl) ... HPLC and matrix assisted laser desorption mass spectrometry (MALDI-MS) Synthesis and characterization of pHPMA The HPMA copolymer used in this study was described by Lammers [39] The weight- and...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
Ngày tải lên :
19/02/2014, 05:20
... E-cadherin distribution, cell scattering and uPA expression (A) T47D cells (a and b) and MCF7 cells (c and d) were treated for h with control or Decma supernatant and immunostained with anti-E-cadherin ... (a and b) and MCF7 cells (c and d) were grown for days to 60–70% confluence and then treated with control or Decma supernatant for h before recording (C) MCF7 cells were treated with Decma and ... LY294002 and cytochalasin D (CytD) was from Sigma-Aldrich GmbH (Basel, Switzerland), SP600125 was obtained from Biomol (Wangen, Switzerland), and TPA, horseradish peroxidase-conjugated antimouse and...
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