small but critical regulators of cancer stem cells

... size of tumour, because of the much smaller number of stem cells, clonal evolution is predicted to be faster in stem cells compared to non -stem cells What are the mechanisms by which one cancer ... by comparison of the relative strength of selection with non -stem cells could furthermore provide a method of testing the relative strength of selection of stem cells and non -stem cells and thereby ... representing a tumour of a given size The number of stem cells is calculated by assuming one stem cell per 57 000 non -stem cells [28] A greater proportion of stem cells would result in a less dramatic difference...

Ngày tải lên: 13/08/2014, 16:20

... P63+ basal /stem cells were transformed into AR+ or ARcancer cells but rarely into ChgA+ neuroendocrine cancer cells Conventional human prostate cancer cells include malignant cells of luminal, ... basal/ stem cells Patch1 and Smo were found highly expressed in the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of ... biology of the prostate: stem cell biology Urology 2003, 62:11-20 Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate...

Ngày tải lên: 10/08/2014, 05:21

... Page 13 of 13 18 Seo DC, Sung JM, Cho HJ, Yi H, Seo KH, Choi IS, Kim DK, Kim JS, ElAty AMA, Shin HC: Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells Mol Cancer ... in a variety of malignant tumor cells In particular, AK1C1, and AK1C3 are reported in human non -small cell lung carcinoma (A549) cells [21], and a high expression of AL1A1 in lung cancer cell ... pathway, is effective to malignant tumor cells with AL1A [29] AL1A1 would be not only cancer stem cell markers, but also an attractive target of treatment of LCNEC In addition to statistically sorting...

Ngày tải lên: 10/08/2014, 09:22

... According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived somatic stem cells Therefore, markers of “normal” stem cells are being sought with the expectation ... expressing profile of those miRNAs in SP fractions between HCC cells and fetal liver cells Discussion There is a growing realization that many cancers may harbor a small population of cancer stem cells ... neoplastic mechanism of liver cancer stem cells Conclusions This work provides an in vivo model for the study of mechanisms of neoplastic transformation of liver cancer stem cells by separately...

Ngày tải lên: 10/08/2014, 10:20

... al Journal of Experimental & Clinical Cancer Research 2010, 29:174 http://www.jeccr.com/content/29/1/174 cancer stem cells http://www.mirbase.org/ Thus, the identification of cancer stem cell-related ... regulatory factor in cancer cells, even cancer stem cells Another example is miR21, which has been reported to have extensive roles and is expressed in embryonic stem cells [29], neuronal cells [30] and ... microarrays for BCSC cells Table S1-MiRNAs microarray- based miRNAs expression profile of MCF-7 cells (signal value ≥800) The table shows the miRNAs expression profile of MCF-7 cells obtained through...

Ngày tải lên: 10/08/2014, 10:20

... primitive cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells ... comprising less than 0.1% of the tumor mass, has many of the properties of cancer stem cells [4] In particular, self renewal, extended lifespan (compared to normal stem cells) , a high invasive ... in one out of two Gleason cultures tested, and is associated with lethal prostate cancer [24], further study of larger samples of prostate cancer stem cells from different classes of therapy-resistant...

Ngày tải lên: 14/08/2014, 08:21

... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in...

Ngày tải lên: 23/03/2014, 07:20

... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells 13 Gene ... and Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells of Cynomolgus 30 Isolation, Characterization, and Differentiation of Human Embryonic Stem Cells HIROFUMI SUEMORI NORIO...

Ngày tải lên: 11/04/2014, 00:32

... immunohistochemical staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... 7042014 of the National Science Foundation of Beijing, China, the 50573044 of the National Natural Science Foundation of China and the 2005CB623905 of the National Basic Research Program of China ... protruding processes Immunofluorescence staining of β-tubulin, GFAP and GalC demonstrated positive expressions in a substantial number of cells, suggesting that neurosphere-derived cells were able to...

Ngày tải lên: 18/06/2014, 15:20

... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... the event of autologous implantation, but induced pluripotent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are ... here lies in implantation of hepatic stem cells, or in implantation of hepatic cells obtained by differentiation of a different type of stem cell, such as mesenchymal stem cells Other applications,...

Ngày tải lên: 18/06/2014, 16:20

... Origin of cancer stem cells Self-renewal and differentiation potentials are the features of stem cells Origin of cancer stem cells Self-renewal and differentiation potentials are the features of stem ... stem cells, progenitor cells, mature cells, and cancer cells The genetic program controlling self-renewal and differentiation plays a key role in the genesis of cancer stem cells (Figure 1) Cancer ... hypothesis is that cancer stem cells are caused by transforming mutations occurring in multi-potential stem cells, tissue-specific stem cells, progenitor cells, mature cells, and cancer cells described...

Ngày tải lên: 20/06/2014, 07:20

... phenomenon of MSCs was confirmed in different experimental systems [2,12] Other cell types, such as umbilical cord matrix stem cells (UCMS) [14], neural stem cells [15,16] and endothelial progenitor cells ... localization advantage, compared to unmodified cells Given complexity of the processes of biodistribution and homing of injected cells, we reasoned that an effect of engineered cell targeting would be ... delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells Stem Cells 2007, 25(7):1618-26 12 Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L: Mesenchymal progenitor cells...

Ngày tải lên: 20/06/2014, 07:20

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Ngày tải lên: 07/08/2014, 18:21

... accelerated degeneration of the treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected into tissue ... fluorescent protein (GFP)-infected cells Following identification of the position of injection site and the presence of GFP-labelled cells, serial sections in the area of the injection site were mounted...

Ngày tải lên: 09/08/2014, 01:22

... L-selectin)/lymphohematopoietic stem and progenitor cells, small- vessel endothelial cells, embryonic fibroblasts Augments B responses/ plasma cells Growth, differentiation, and isotype-switching of B cells/ B cells Ligand ... mesenchymal stem cells and neural progenitor cells Haematologica 2003, 88:126-133 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells ... infused in the 32nd week of pregnancy After an uneventful pregnancy a bone biopsy performed at months of age demonstrated engraftment of Y chromosome positive cells (7.4% of total cells) [52] These...

Ngày tải lên: 09/08/2014, 08:23

... Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) ... 39 40 41 42 43 44 45 46 47 48 49 characterization of mesenchymal stem cells Stem Cells 2006, 24:475-481 Charo IF, Ransohoff RM: The many roles of chemokines and chemokine receptors in inflammation ... gene-expression profile of mesenchymal stem cells (MSCs; n = 2) was analysed by real-time PCR during their differentiation towards chondrocytes in micropellets (a) Time-course expression of the members of the...

Ngày tải lên: 09/08/2014, 10:20

... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently ... promote but cannot shorten the time of the neural induction from ES cells The Sox1-GFP+ cells did not appear on differentiation day 2, regardless of the FGF1 treatment The increase of Sox1+ cells ... inhibition of JNK1, but not JNK2, sig- Page of 11 nificantly reduced the formation of Sox1+ and N-cadherin+ cells (Fig 5C, 5D and 5E), indicating that JNK1 is essential for the neural induction of ES cells...

Ngày tải lên: 10/08/2014, 05:21

... Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... of the characteristics of HPBAML-I are not typically observed in leukemic cells, we wondered whether HPB-AML-I cells are neoplastic cells originating from the non-hematopoietic compartments of ... Grünwald-Giemsa staining (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle-like cells Unlike the round-polygonal cells, HPB-AML-I cells with the spindle-like...

Ngày tải lên: 10/08/2014, 10:20