slow intermediate and fast progressors

... and 32%, respectively), consistent with at least some being DCs The CD4+CD11b- and CD4-CD11b- subsets have similarities in expression of CD11c (75% and 65%), CD54 (70% and 90%), CD172a (60% and ... PE-OX6, FITCWT5, and Cy-chrome-OX35 and were washed and fixed with 1% paraformaldehyde Flow cytometry Labelled cells were analysed with a Beckman Coulter EPICS XL-MCL flow cytometer and Coulter EXPO ... CD11c (75% and 65%), CD54 (70% and 90%), CD172a (60% and 66%), CD11a (60% and 96%), CD32 (50% and 95%), and/ or CD5 (20% and 32%) The two subsets also have similarities with putative MHC II+CD4CD11b-CD11c+...

Ngày tải lên: 09/08/2014, 10:22

Ngày tải lên: 18/03/2014, 11:20

... bovine serum albumin, and 0.05% Tween (for CXCR2 and CXCR3) The primary antibodies against CXCR1, CXCR2, and CXCR3 were the same as above and used at 1:100 (CXCR1 and CXCR2) and 1:80 (CXCR3) in ... the clinical diagnosis and to ensure typical pathologic characteristics of RA and OA Infiltration of T as well as B cells and their organization into lymphatic aggregates and follicular structures ... CAA ACT-3′ and 5′-GAG CAG GAA GAT GAG GAC GAC-3′, at 58°C annealing temperature and for 33 cycles, were used for CXCR2-specific amplification; and 5′-GCT TTG ACC GCT ACC TGA ACA-3′ and 5′-GGC...

Ngày tải lên: 09/08/2014, 01:23

... contributions AS and DD designed this study and carried out immnunohistochemistry staining, western blotting and RT-PCR and drafted the manuscript LM, and KB, provided the clinical samples and collected ... CI: 2.28-24.5) when benign or intermediate tumor is the reference [Table 3] The sensitivity and the specificity of STAT3 were 95.8% and 76.5% and pSTAT3 were 50% and 88.2%, respectively, with ... Expression of immunohistochemical markers, STAT3 (A, C, E) and p-STAT3 (B, D, F), in benign (A and B); intermediate (C and D); malignant (E and F) soft tissue tumors The nuclei were counterstained...

Ngày tải lên: 10/08/2014, 10:21

... work and the data analysis, participated in the design of the study and drafted the manuscript JGP and JM participated in the design of the study and in manuscript preparation All authors read and ... plants and yeast [22] and are the most abundant type in plants [4] Plant R2R3MYBs take part in many biological processes including seed development and germination [23], the stress response [24] and ... phenylpropanoid and lignin biosynthesis [5,8-11] and vascular organisation [3] (for a review, see Ref [25]) The genetic selection and breeding activities of a few commercial conifer species are being expanded...

Ngày tải lên: 12/08/2014, 05:20

... 3β-androstenol sulfate HO 5α-androsten-3α-ol (α-androstenol) hydroxy-androstenone sulfate 3αHSD O androstenone hydroxy-androstenone hydroxysteroid sulfotransferase andro 3-enol sulfate andro 3-enol Figure ... 5,16-androstadien-3β-ol O 4,16-androstadien-3-one Final products of androstenone sulfonation 5α-androsten-3β-ol (β-androstenol) SRD5A 3βHSD 3α-androstenol sulfate hydroxysteroid sulfotransferase 3β-androstenol ... cholesterol CYP21 andien-β synthetase (CYB5, CYP17…) 11-deoxycorticosterone 11- 4,16-androstadien-3-ene 4,16-androstadien- 3βHSD androstadienol sulfate hydroxysteroid sulfotransferase HO 5,16-androstadien-3β-ol...

Ngày tải lên: 14/08/2014, 13:21

... detected for androstenone [21] GENES INVOLVED IN THE METABOLISM OF ANDROSTENONE 3.1 Androstenone synthesis and metabolism Together with other 16-androstene steroids, androstenone (5 α-androst-16en-3-one) ... 5α-androsten-3α-ol α-androstenol 5α-androsten-3β-ol β-androstenol HO 5,16-androstadien-3β-ol andien-β synthetase (CYB5, CYP17…) HO Figure Main enzymes involved in the porcine metabolism of androstenone ... skatole SSC6, SSC13 and SSC14 [21] versus SSC7, SSC12 and SSCX [4] and for indole SSC13 and SSC14 [21] versus SSC2, SSC6 and SSC7 [4] The results of the assessment of lean and fat samples by a...

Ngày tải lên: 14/08/2014, 13:22

... anisomycin and FBS on actin stress fibers in SMCs and their modulation by toxin B and latrunculin B Cells were first stimulated with either S1P, anisomycin or FBS for 30 and then fixed, permeabilized and ... the indicated periods and the amount of GTP-loaded RhoA, Cdc42 and Rac1 was determined by pull-down assay as described in Materials and methods Total amount of RhoA, Cdc42 and Rac1 in the same ... containing actinomycin D (10 lgÆmL)1) and the cells were harvested after 0, 0.5, 1, and h Total RNA was purified and analyzed by northern blot hybridization and phosphorimaging densitometry The...

Ngày tải lên: 19/02/2014, 16:20

... lesions on a standard chow diet It is used for studies of hyperlipidemia and atherosclerosis, and has been used extensively in understanding the mechanisms of lipoprotein metabolism and atherosclerosis ... (eWAT, rWAT, and iWAT, left), and brown adipose depots (iBAT, and mBAT, right) by real-time PCR n = *P < 0.05 vs FA Xu et al Particle and Fibre Toxicology 2011, 8:20 http://www.particleandfibretoxicology.com/content/8/1/20 ... ES017412, and ES018900 to Dr Sun, ES015146 and ES017290, and EPA grant R834797-01 to Dr Rajagopalan, an NPACT Initiative grant from the Health Effects Institute to Drs Lippmann and Chen, and laboratory...

Ngày tải lên: 06/03/2014, 19:20

... sPLA2-IID (mIID) and its mutants, KE2 and KE2RS2 Two and four basic amino acids are replaced by glutamic acid or serine in KE2 and KE2RS2, respectively (B) Expression of the wild-type (WT) and two mutants ... regulatory mechanisms for gene expression, and perhaps functions, of sPLA2-IID and those of sPLA2-IIA and -V are not entirely identical and are even cell- and tissue-specific Searching the nucleic ... subfamily (types IIA and V) and type X secretory phospholipase A2s in regulating arachidonic acid release and prostaglandin generation: implication of cyclooxygenase-2 induction and phospholipid...

Ngày tải lên: 18/03/2014, 01:20

... T-046071 and T-067963 to J Ovadi, T-049247 ´ to F.O., and PD 76793 to J Ovadi; FP6-2003-LIFESCIHEALTH-I: BioSim and NKFP-MediChem2 ⁄ A ⁄ ´ 005 ⁄ 2004 to J Olah; and by RET-NORT 08 ⁄ 2004 and ETT ... obtained from an integrated experimentation and modelling reveal and suggest relations between the changes in morphology, glycolytic flux, ATP production and ATP levels The mathematical models described ... 12–16 weeks of age, and exhibited an uncoordinated gait, hypoactivity, stereotypic movements and shakinglike tremor In the end-stage (20–24 weeks of age), the mice lost weight and appeared to be...

Ngày tải lên: 23/03/2014, 10:20

... dilution Bands correspond to 70 kDa (aralar1 and citrin) and 52 kDa for b-F1ATPase (C) Western blot analysis of aralar1 and citrin in auricular and ventricular myocardium 20 lg of atria (A) and ventricle ... 5000) and anti-(b-F1ATPase) (1 : 5000), stripped and probed again with anti-(citrin N-terminus) (1 : 2000) The amount of aralar1 (standardized to that of b-F1ATPase) was 1.5 and 0.9 in atria and ... atria and ventricle are observed for citrin levels (0.86 and 0.64 standardized values, in atria and ventricles, respectively) (D) Immunoblotting of increasing amounts of recombinant citrin and...

Ngày tải lên: 24/03/2014, 04:21

... GPR30, a novel estrogen receptor, and ERα stimulation by both G-1 (GPR30-specific ligand) and estradiol were shown to synergistically induce proliferation of breast and ovarian cancer lines [15] ... benign, and malignant ovarian tissues To investigate the role of estrogen and GPR30 in STYK1 regulation, we treated a benign and several malignant ovarian cancer cell lines with estradiol and G-1 ... HS832, OvCar5, and SkOv3 cells treated (16 hours) with G-1, a GPR30-specific ligand, showed an increase in Discussion and Conclusion STYK1 mRNA levels have been reported in human benign and/ or malignant...

Ngày tải lên: 20/06/2014, 07:20

... the results, and prepared the draft of the manuscript DM analyzed the RBS and ERDA measurement results FD and BSS carried out the PL study MC and FD carried out the TEM studies and investigated ... center) and positively (K+ center) charged silicon atoms as traps for holes and electrons, respectively [48], and third to Si-Si bond or nitrogen vacancy possibly being responsible for electron and ... power of 500 W and an RF power of W Page of 10 at 300°C Rutherford backscattering (RBS) and elastic recoil detection analysis (ERDA) techniques were used to determine the Si/N ratio and hydrogen...

Ngày tải lên: 21/06/2014, 05:20

Ngày tải lên: 07/08/2014, 18:21

... detected between I–30, on the one hand, and N and C:N, on the other In January and February, these correlations were even closer in contrast to those of glucose and fructose, whose concentrations ... Block et al (1995) [7] and Gieger and Thomas (2002) [14] Although the overall correlations between N or C:N, respectively, and I–30 were not very tight, and although N and C:N did not contribute ... variable and concentrations of individual and total soluble sugars, quercitol, N and C:N as independent variables The analyses were calculated for the entire investigation period (October–April), and...

Ngày tải lên: 08/08/2014, 01:21

... (1 l j − i) and Y = Y (x, zj−i), where z0 = y (7.11) PY satisfies (UD)i in ΓY for all Y ∈ P and i Proof Let x, y ∈ Y and set Z = Y (x, y) ∈ P Then Y ∩ Z belongs to PY and contains x and y, so that ... rank(y) = d and x covers x∧y, there are exactly α+1 pairs (x1 , y1) such that y1 covers both x and x1 , and y covers x1 We say P is regular if P is line regular, dual-line regular and zig-zag ... descendent of H(n, 2) Then n = 2d and one of the following holds: (i) w = and Y = {x ∈ X : wt(x − z) = 1} for some z ∈ 1+ε Xn = Fn \X; (ii) w = d − and there are a ∈ F2 and i ∈ {1, 2, , n} such...

Ngày tải lên: 08/08/2014, 14:23

... laboratory, and statistical evaluation of material from RA patients; and preparation of the manuscript LD performed the collection and clinical analysis of patients with degenerative joint diseases and ... properties and thereby modulate MMP9 activity Finally, the presence of TIMP-specific autoimmunity is associated with a nondestructive course of RA R1015 Materials and methods Patients and controls ... 42 patients were receiving no DMARD treatment at the time of blood and synovial fluid sampling Recent radiographs of the hands and feet were obtained for all the patients The presence of bone erosions,...

Ngày tải lên: 09/08/2014, 06:23

... quantify and characterise the distribution and phenotype of mDCs and pDCs in blood from PsA patients and RA patients in comparison with osteoarthritis (OA) patients and healthy donors and synovial ... patients and RA patients We also investigated the functional capabilities of mDCs and pDCs isolated from SF to mature and release cytokines upon TLR stimulation Materials and methods Patients and ... enumerated mDCs and pDCs in SF and matched PB obtained from six patients with RA and from six patients with PsA Magnetic sorting and flow cytometry revealed that, whereas absolute mDC and pDC numbers...

Ngày tải lên: 09/08/2014, 07:20

... colonystimulating factor, GM-CSF, and the levels of IgM were increased, and IL-1β and TNF were detected Direct contact with the B-cell clone was required for RA-NLCs to produce IL-1β and TNF and higher levels ... thymocytes and to also play a role in positive/negative selection of T cells in mouse and rat thymus A very similar capacity to interact and support the maturation of some population of lymphocytes and ... the ability to secrete large amounts of cytokines and chemokines that could promote the accumulation and activation of lymphocytes and monocytes, and their unique capacity to promote the differentiation...

Ngày tải lên: 09/08/2014, 08:23