... b-cyclodextrin injection, and the bottom panel depicts the binding isotherm; open circles represent the integrated binding heat of the data in the top panel, and the full line is the fit of a one-site binding ... showed no binding to maltoheptaose Taken together, the data presented here show that the binding affinity of the 1180 The full-length AtAMY3 offered an advantage when examining the binding affinity ... the starch-binding ability of AtAMY3 in vitro It cannot be precluded that some binding may involve secondary binding sites in the catalytic domain, but the demonstrated starchbinding ability...
Ngày tải lên: 06/03/2014, 00:21
... stages in the biological cycle of the parasite (i.e the epimastigote and the metacyclic trypomastigote in the insect vector, and the intracellular amastigote and the bloodstream trypomastigote in the ... Arg141¢ The other end of the substrate points into a deep pocket in the enzyme Moving along the ligand from the phosphate, O4 interacts with His102¢ and a water molecule that is in turn within hydrogen-bonding ... contacts with their equivalents in the other subunit of the second dimer (Fig 4A) In the case of P21212 (TcRpiB-R5P), the four molecules in the asymmetric unit represent the tetramer The two active...
Ngày tải lên: 15/03/2014, 00:20
Sleep disorders in the elderly pot
... insomnia include sleep onset insomnia (difficulty initiating sleep) , sleep maintenance insomnia (difficulty maintaining sleep throughout the night), early morning insomnia (early morning awakenings ... usually begins with conducting a complete sleep history focusing on EDS, unintentional napping, snoring, and other sleep disorder symptoms If possible, obtaining information from the patient’s sleep ... fall asleep within 20 min, they are asked to repeat the process Sleep restriction therapy aims to increase sleep efficiency by limiting the amount of time the patient is allowed to stay in bed...
Ngày tải lên: 22/03/2014, 14:20
Attitudes, barriers and facilitators for health promotion in the elderly in primary care doc
... group interviews in Geneva, the interview guide was translated into French As an introduction to the discussion, we asked questions concerning the opinion of the GPs about health promotion in the ... important themes of health promotion in the elderly Some of the GPs complained of a lack regarding the content but also regarding specific skills in health promotion due to the fact that they had ... health promotion programmes for the elderly This contains the accessibility in a regional sense as well as regarding the content of the programme Providing information over the internet for instance,...
Ngày tải lên: 22/03/2014, 14:20
Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases potx
... domain preceding the peptidase C11 domain and a bronectin type domain lying between the two The picture that emerges is one in which clostripain itself, the only member of the peptidase C11 family ... mechanisms [42,44] The study of the effect of pH on enzyme inactivation allows the calculation of the pKa of the His176 side chain involved in the inactivation reaction The rate of inactivation exhibited ... and, since our interest lay principally in understanding peptidase activity in the clostripain family, was not studied further The appearance of inactivated copies of related peptidases in various...
Ngày tải lên: 23/03/2014, 12:20
báo cáo khoa học: " MoccaDB - an integrative database for functional, comparative and diversity studies in the Rubiaceae family" pdf
... integrated the bioinformatics tools in the application VP served as the principal investigator of the project, performed the data analysis, assisted in the designing of the database, and drafted the ... funding from the IRD-SPIRALES Board AdK and SH coordinated the project CT managed the project development, assisted in the designing of the database, performed database system administration, integrated ... used in large-scale genomic studies owing to their ubiquitous distribution in both protein-coding and non-coding regions and the high degree of length polymorphism among indi- viduals [5] The...
Ngày tải lên: 12/08/2014, 03:21
29819 rooms in the house family members
... h.Where is her sister? i Where is her brother? _ j Where is grandpa? ...
Ngày tải lên: 27/08/2016, 14:55
Tài liệu Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx
... the results obtained in the crayfish Orconectes limosus, showing that l-isomer of CHH is always present in the different parts of the d-CHH cells, in decreasing amounts from the cell body to the ... immunocytochemical results obtained in the present study invalidate our starting hypothesis proposing that the cells coexpressing VIH and CHH in the lobster X organ, as observed in previous studies [26,42], ... microscopy study of the neuronal endings in the sinus gland to determine the subcellular localization of the different epimers Taken together, these results allow a discussion of the existence of...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx
... the need of other proteins to stabilize the proteins in the membrane The interaction of Ga subunits with Gbc could be a stabilizing factor Mutations on Gaq residues which locate in the Gbc binding ... enhancement in the rate of degradation compared with the wild-type protein Our data is more consistent with an increased destabilization of the protein in the cytoplasm, a process that, in the case of the ... experiments the turnover rate of the proteins, thus excluding their involvement in protein breakdown On the contrary, treatment with the proteasome inhibitors MG132 5368 (50 lm) and the highly...
Ngày tải lên: 20/02/2014, 03:20
Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... The introduction of mutation into one of the putative Etsbinding sequences suppressed the promoter activity In addition, the specific binding of a nuclear protein to the oligonucleotide containing ... assay (EMSA) using the Ets consensus site at )133 As the involvement of the Ets consensus binding site at )133 in the promoter activity of the mouse a3 integrin gene was suggested by the luciferase ... a3b1 integrin plays essential roles in multiple processes during embryogenesis The promoter should thus contain elements directing the expression of this integrin in the kidney, lung, and skin...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu recent trends in the law and policy of bioenergy production promotion and use docx
Ngày tải lên: 21/02/2014, 05:20
Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... position 366 while all other proteins of the CYP11B family including CYP11B2 of the guinea pig have a basic residue (arginine or lysine, respectively) at the corresponding position These two basic residues ... 6) for the remaining introns Finally, the 3¢ UTRs of the guinea pig CYP11B genes show only 42% similarity whereas the 3¢UTR of the guinea pig CYP11B2 gene shares up to 72% identity with the corresponding ... abrogated almost completely These results indicate clear structural differences on the surface of these proteins involved either in glucocorticoid or in mineralocorticoid biosynthesis despite a high...
Ngày tải lên: 22/02/2014, 07:20
Báo cáo khoa học: Evolutionary origin and divergence of PQRFamide peptides and LPXRFamide peptides in the RFamide peptide family pdf
... cells expressing lamprey PQRFamide peptide mRNA in the brain must be taken into account when studying the neuropeptide action We therefore characterized the regions in the brain showing the expression ... system [40] These mammalian results indicate that NPFF may be involved in sensory transmission in the spinal cord, including pain systems, autonomic regulation in the medulla, and neuroendocrine regulation ... in the olfactory bulb and the nucleus olfactoretinalis in the Novel lamprey RFamide peptides telencephalon, but is absent in more caudal regions, including the hypothalamus, brainstem and spinal...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... 1), thereby confirming the crucial role of Asp142 Catalysis in family 18 chitinases (Eur J Biochem 271) 259 In most ChiB structures, Asp142 is clearly defined, being either in the ÔdownÕ or in the ... that the carboxylic groups in the Asp140-Asp142-Glu144 triad contain two protons at the pH where the enzyme is most active The basic arm of the pH-activity profile must be determined by the loss ... for the wildtype enzyme, Glu144 is the only relevant titratable residue in the pH 6–12 range and the pKa of this residue is affected by substratebinding Therefore, it is surprising that the pH-kcat...
Ngày tải lên: 07/03/2014, 14:20
Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
... activity like other MACS family proteins The recombinant plasmid carrying either the rat o-macs or the murine MACS1 cDNA was transfected into HEK293T cells The recombinant proteins contained the c-Myc ... to the murine acetyl-CoA synthetase (AceCS) family was much less (20–30%) These observations indicate that the O-MACS protein is a novel member of the acyl-CoA synthetase family, belonging to the ... like Rat IGF binding protein Rat FGF receptor activating protein Rat PKC delta-binding protein Rat cellular retinoyl-binding protein Mouse rho (NET1A) like Rat RYF3 (vomeromodulin) Rat RYA3 potential...
Ngày tải lên: 08/03/2014, 02:20
Health Promotion for the Elderly in Peru, Bolivia and Chile ppt
... Report series of Master theses in Public Health These reports come from the Board of Education in Public Health Sciences at Karolinska Institutet The master education of Public Health ... 14 2.3.5 Family- based approaches 15 2.3.6 The individual approach 15 2.4 THEORETICAL MODELS FOR HEALTH PROMOTION IN OLDER PEOPLE 15 2.4.1 Individual-Level ... PROMOTION PROGRAMMES IN THE PAN AMERICA HEALTH ORGANIZATION 25 6.2.1 Elderly People and Family 25 6.2.2 Promotion of Active Life 25 6.3 HEALTH PROMOTION PROGRAMMES IN...
Ngày tải lên: 14/03/2014, 17:20
The Effectiveness of Educational Intervention in the Health Promotion in Elderly people ppt
... distributing educational pamphlets to the elderly: In the initial phase of the study, educational materials were designed and prepared in order to support the volunteers During the initial phase of the ... was meaningful Having a meaningful life and a feeling of happiness in all age groups has increased after the interventions (P= 0.00) In women aged 70 and older, Not being worried about the future, ... and after the interventions In terms of food preferences, in the women's group, in all age groups, the food most preferred before and after the interventions was bread but regarding the second...
Ngày tải lên: 14/03/2014, 20:20
Fertility of Men and Women Aged 15–44 Years in the United States: National Survey of Family Growth, 2006–2010 docx
... 12, 2012 required from the minor: If either the parent or the minor declined to give written consent, the minor did not participate in the survey The response rate for the 2006–2010 NSFG was ... from the NSFG, because mothers report information for the birth registration system Not all mothers report information about the fathers of their babies, and if they do, their reports of the father’s ... Nonetheless, teenage childbearing in the United States cost taxpayers at least $10.9 billion in 2008 (22) Nonmarital childbearing Over the past several decades, nonmarital childbearing has increased...
Ngày tải lên: 22/03/2014, 11:20
Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
... different trypsins in the digestive gland of the spiny lobster Panulirus argus EF1-a was used as the housekeeping gene The same results were obtained when using b-actin as the housekeeping gene (not ... at the junction of the two barrels Another conserved characteristic of lobster trypsins is the presence of calcium-binding sites (Fig 3C) The calcium-binding motif does not occur in many invertebrate ... despite the presence of calcium-binding sites, Ca2+ affected neither the activity nor the stability of crab trypsin because there are no accessible autolysis sites in the N-terminal domain, which...
Ngày tải lên: 23/03/2014, 03:20