... b-cyclodextrin injection, and the bottom panel depicts the binding isotherm; open circles represent the integrated binding heat of the data inthe top panel, and the full line is the fit of a one-site binding ... showed no binding to maltoheptaose Taken together, the data presented here show that the binding affinity of the 1180 The full-length AtAMY3 offered an advantage when examining the binding affinity ... the starch-binding ability of AtAMY3 in vitro It cannot be precluded that some binding may involve secondary binding sites inthe catalytic domain, but the demonstrated starchbinding ability...
... stages inthe biological cycle of the parasite (i.e the epimastigote and the metacyclic trypomastigote inthe insect vector, and the intracellular amastigote and the bloodstream trypomastigote inthe ... Arg141¢ The other end of the substrate points into a deep pocket inthe enzyme Moving along the ligand from the phosphate, O4 interacts with His102¢ and a water molecule that is in turn within hydrogen-bonding ... contacts with their equivalents inthe other subunit of the second dimer (Fig 4A) Inthe case of P21212 (TcRpiB-R5P), the four molecules inthe asymmetric unit represent the tetramer The two active...
... insomnia include sleep onset insomnia (difficulty initiating sleep) , sleep maintenance insomnia (difficulty maintaining sleep throughout the night), early morning insomnia (early morning awakenings ... usually begins with conducting a complete sleep history focusing on EDS, unintentional napping, snoring, and other sleep disorder symptoms If possible, obtaining information from the patient’s sleep ... fall asleep within 20 min, they are asked to repeat the process Sleep restriction therapy aims to increase sleep efficiency by limiting the amount of time the patient is allowed to stay in bed...
... group interviews in Geneva, the interview guide was translated into French As an introduction to the discussion, we asked questions concerning the opinion of the GPs about health promotioninthe ... important themes of health promotioninthe elderly Some of the GPs complained of a lack regarding the content but also regarding specific skills in health promotion due to the fact that they had ... health promotion programmes for the elderly This contains the accessibility in a regional sense as well as regarding the content of the programme Providing information over the internet for instance,...
... domain preceding the peptidase C11 domain and a bronectin type domain lying between the two The picture that emerges is one in which clostripain itself, the only member of the peptidase C11 family ... mechanisms [42,44] The study of the effect of pH on enzyme inactivation allows the calculation of the pKa of the His176 side chain involved inthe inactivation reaction The rate of inactivation exhibited ... and, since our interest lay principally in understanding peptidase activity inthe clostripain family, was not studied further The appearance of inactivated copies of related peptidases in various...
... integrated the bioinformatics tools inthe application VP served as the principal investigator of the project, performed the data analysis, assisted inthe designing of the database, and drafted the ... funding from the IRD-SPIRALES Board AdK and SH coordinated the project CT managed the project development, assisted inthe designing of the database, performed database system administration, integrated ... used in large-scale genomic studies owing to their ubiquitous distribution in both protein-coding and non-coding regions and the high degree of length polymorphism among indi- viduals [5] The...
... the results obtained inthe crayfish Orconectes limosus, showing that l-isomer of CHH is always present inthe different parts of the d-CHH cells, in decreasing amounts from the cell body to the ... immunocytochemical results obtained inthe present study invalidate our starting hypothesis proposing that the cells coexpressing VIH and CHH inthe lobster X organ, as observed in previous studies [26,42], ... microscopy study of the neuronal endings inthe sinus gland to determine the subcellular localization of the different epimers Taken together, these results allow a discussion of the existence of...
... the need of other proteins to stabilize the proteins inthe membrane The interaction of Ga subunits with Gbc could be a stabilizing factor Mutations on Gaq residues which locate inthe Gbc binding ... enhancement inthe rate of degradation compared with the wild-type protein Our data is more consistent with an increased destabilization of the protein inthe cytoplasm, a process that, inthe case of the ... experiments the turnover rate of the proteins, thus excluding their involvement in protein breakdown On the contrary, treatment with the proteasome inhibitors MG132 5368 (50 lm) and the highly...
... The introduction of mutation into one of the putative Etsbinding sequences suppressed the promoter activity In addition, the specific binding of a nuclear protein to the oligonucleotide containing ... assay (EMSA) using the Ets consensus site at )133 As the involvement of the Ets consensus binding site at )133 inthe promoter activity of the mouse a3 integrin gene was suggested by the luciferase ... a3b1 integrin plays essential roles in multiple processes during embryogenesis The promoter should thus contain elements directing the expression of this integrin inthe kidney, lung, and skin...
... position 366 while all other proteins of the CYP11B family including CYP11B2 of the guinea pig have a basic residue (arginine or lysine, respectively) at the corresponding position These two basic residues ... 6) for the remaining introns Finally, the 3¢ UTRs of the guinea pig CYP11B genes show only 42% similarity whereas the 3¢UTR of the guinea pig CYP11B2 gene shares up to 72% identity with the corresponding ... abrogated almost completely These results indicate clear structural differences on the surface of these proteins involved either in glucocorticoid or in mineralocorticoid biosynthesis despite a high...
... cells expressing lamprey PQRFamide peptide mRNA inthe brain must be taken into account when studying the neuropeptide action We therefore characterized the regions inthe brain showing the expression ... system [40] These mammalian results indicate that NPFF may be involved in sensory transmission inthe spinal cord, including pain systems, autonomic regulation inthe medulla, and neuroendocrine regulation ... inthe olfactory bulb and the nucleus olfactoretinalis inthe Novel lamprey RFamide peptides telencephalon, but is absent in more caudal regions, including the hypothalamus, brainstem and spinal...
... 1), thereby confirming the crucial role of Asp142 Catalysis infamily 18 chitinases (Eur J Biochem 271) 259 In most ChiB structures, Asp142 is clearly defined, being either inthe ÔdownÕ or inthe ... that the carboxylic groups inthe Asp140-Asp142-Glu144 triad contain two protons at the pH where the enzyme is most active The basic arm of the pH-activity profile must be determined by the loss ... for the wildtype enzyme, Glu144 is the only relevant titratable residue inthe pH 6–12 range and the pKa of this residue is affected by substratebinding Therefore, it is surprising that the pH-kcat...
... activity like other MACS family proteins The recombinant plasmid carrying either the rat o-macs or the murine MACS1 cDNA was transfected into HEK293T cells The recombinant proteins contained the c-Myc ... to the murine acetyl-CoA synthetase (AceCS) family was much less (20–30%) These observations indicate that the O-MACS protein is a novel member of the acyl-CoA synthetase family, belonging to the ... like Rat IGF binding protein Rat FGF receptor activating protein Rat PKC delta-binding protein Rat cellular retinoyl-binding protein Mouse rho (NET1A) like Rat RYF3 (vomeromodulin) Rat RYA3 potential...
... Report series of Master theses in Public Health These reports come from the Board of Education in Public Health Sciences at Karolinska Institutet The master education of Public Health ... 14 2.3.5 Family- based approaches 15 2.3.6 The individual approach 15 2.4 THEORETICAL MODELS FOR HEALTH PROMOTIONIN OLDER PEOPLE 15 2.4.1 Individual-Level ... PROMOTION PROGRAMMES INTHE PAN AMERICA HEALTH ORGANIZATION 25 6.2.1 Elderly People and Family 25 6.2.2 Promotion of Active Life 25 6.3 HEALTH PROMOTION PROGRAMMES IN...
... distributing educational pamphlets to the elderly: Inthe initial phase of the study, educational materials were designed and prepared in order to support the volunteers During the initial phase of the ... was meaningful Having a meaningful life and a feeling of happiness in all age groups has increased after the interventions (P= 0.00) In women aged 70 and older, Not being worried about the future, ... and after the interventions In terms of food preferences, inthe women's group, in all age groups, the food most preferred before and after the interventions was bread but regarding the second...
... 12, 2012 required from the minor: If either the parent or the minor declined to give written consent, the minor did not participate inthe survey The response rate for the 2006–2010 NSFG was ... from the NSFG, because mothers report information for the birth registration system Not all mothers report information about the fathers of their babies, and if they do, their reports of the father’s ... Nonetheless, teenage childbearinginthe United States cost taxpayers at least $10.9 billion in 2008 (22) Nonmarital childbearing Over the past several decades, nonmarital childbearing has increased...
... different trypsins inthe digestive gland of the spiny lobster Panulirus argus EF1-a was used as the housekeeping gene The same results were obtained when using b-actin as the housekeeping gene (not ... at the junction of the two barrels Another conserved characteristic of lobster trypsins is the presence of calcium-binding sites (Fig 3C) The calcium-binding motif does not occur in many invertebrate ... despite the presence of calcium-binding sites, Ca2+ affected neither the activity nor the stability of crab trypsin because there are no accessible autolysis sites inthe N-terminal domain, which...