sjögren s syndrome maria c gonzalez mayda and amy joseph

... GC-like structures drome (SS) (a–d) salivary gland ducts of sialoadenitis (e,f) IL-18 expression inand nonspecific chronicpatients with Sjögren' s syndrome (SS) (a–d) and nonspecific chronic sialoadenitis ... normal and diseased controls, and its relationship with autoantibody production IL-18 is increased in the systemic circulation of autoimmune diseases such as RA and Crohn 's disease, in which the ... with Sjögren' s syndrome (SS) (a c, g–i) and in nonspecific chronic sialoadenitis (d–f) sialoadenitis (d–f) (a,b) Paraffin-embedded section of glands in SS, showing high amounts of IL-18-expressing...

Ngày tải lên: 09/08/2014, 01:23

... lymphoid tissues of pSS B cells Isotype class switching is a complex multistep process that requires close collaboration between surface IgM-expressing B cells and CD4+ helper T cells in the environment ... B cells show a higher intrinsic transmigratory capacity to CXCL12 and CXCL13 than CD27– naive B cells [26] Thus, glandular coexpression of both CXCL12 and CXCL13 [28-31] seems to direct this subpopulation ... healthy donors [26] Thus, CXCL12–CXCR4 and CXCL13–CXCR5 interactions have been strongly suggested to be of special importance in B-cell disturbances in pSS and may be closely associated with the...

Ngày tải lên: 09/08/2014, 10:20

... BAFF/β-actin values of 269 and 183.8 These patients had no specific clinical features; the reassessment of BAFF mRNA levels in these patients confirmed these values BAFF polymorphism was not associated ... BAFF -871 T /C polymorphism and serum BAFF level in 95 patients with pSS The bar represents the standard error BAFF = B-cell-activating factor; pSS, primary Sjögren' s syndrome Discussion level ... 40 cycles at 95 C for 10 s, 60 C for 15 s and 72 C for 20 s For each run, serially diluted cDNA of K562 cells was used as a quantitative standard To correct for variations in mRNA recovery and...

Ngày tải lên: 09/08/2014, 07:20

... Epidemiologic Studies Depression Scale(CESD) [16] The PROFAD-SSI was also used to assess sicca severity Description of the instruments used The PROFAD-SSI is comprised of eight domain scores that reflect ... devised an extensive health questionnaire: the ASSESS survey (Assessment of Symptoms and Experiences of Sjögren' s syndrome) for this study based on recommendations of a panel of Sjogren 's investigators ... foundation (SSF-PSS) were classified as "possible" PSS if they reported a diagnosis of Sjogren 's syndrome and history of a positive minor salivary gland biopsy and/ or a positive anti-SSA/Ro or anti-SSB/La...

Ngày tải lên: 18/06/2014, 18:20

... cells 60% BBcells cells B cells CD27 Expression CD27 Expression Plasmablasts Naïve Naïve B cells B cells Memory Memory B cells B cells CD27 Expression Schematic distribution of B cell subsets ... there is no conclusive evidence for recombination biases predisposing to systemic autoimmunity in SLE and SS, there are some exceptions In some circumstances, specific autoantibodies express marked ... RI, Kassan SS, Pillemer SR, Talal N, Weisman MH: European Study Group on Classification Criteria for Sjögren s Syndrome Assessment of the European classification criteria for Sjögren s syndrome...

Ngày tải lên: 09/08/2014, 01:21

... pathogenesis In this context, activated epithelial duct cells have been shown to secrete specific chemokines, such as SDF-1 (CXCL-12) and BCA-1 (CXCL-13), that are capable of attracting specific B lymphocytes ... rearrangements of B cells obtained from the salivary glands of SS patients or in B cells from mucosa-associated lymphoid tissue lymphoma in SS patients Furthermore, Stott et al described a decreased R /S ... mutations/base [12] Few if any of the nucleotide changes encountered in this analysis can thus be ascribed to amplification errors Analysis of sequences Sequences were analyzed using the V BASE Sequence...

Ngày tải lên: 09/08/2014, 03:24

Ngày tải lên: 09/08/2014, 10:20

Ngày tải lên: 09/08/2014, 10:20

... pairwise from all analyses except PCA and DA All analyses were computed using SPSS 13 (SPSS Inc., Chicago, IL, USA) Results Autoimmune disease manifestations At 21 weeks of age salivary secretion ... analyses An immune response is an orchestrated process that involves several protein families and several molecules with similar molecular function The PANTHER classification system was used to classify ... (positively associated with salivary flow) was apparent Components associated with FS and/ or RI correlated strongly with each other, indicating a process in which cytokine Sa -C- 2 and CXCL Sa -C- 2...

Ngày tải lên: 09/08/2014, 10:23

... associated with systemic inflammation or excessive risk of thrombosis in various diseases, such as rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), vasculitis and antiphospholipid syndrome ... assay Results are expressed as nM PS Eq Solid bars show the mean poorly assessed in pSS, we assessed plasma levels of sCD40L and sCD62P, two biomarkers of platelet activation (Table 2) The concentration ... systemic lupus erythematosus; SLEDAI = SLE disease activity score (sPLA2) activity and sCD40L and soluble P-selectin (sCD62P) content MP quantification by functional prothrombinase capture assay...

Ngày tải lên: 09/08/2014, 14:22

... including systemic lupus erythematosus (SLE), and myositis [7] Nevertheless, in the 2002 classification standards for SjS diagnosis, positive SSA and SSB autoantibody tests were the only mandatory ... salivary gland biopsy are required for the diagnosis of primary SjS [6] This is because antibodies to SSA and SSB are not specific to SjS, but are also found in other rheumatological diseases ... mandatory criteria if the salivary gland biopsy was negative [6] Current SSA and SSB ELISAs, which employ native antigen complexes isolated from calf thymus, show positive SSA and SSB serology...

Ngày tải lên: 18/06/2014, 15:20

... Kratzsch G: Neurological and neuropsychiatric dysfunction in primary Sjögren s syndrome Acta Neurol Scand 1994, 89:31-35 Cox P, Hales R: CNS Sjögren s syndrome: an underrecognized and underappreciated ... neuropathological mechanisms of SS are unknown In CNS-SS, the most serious neuropsychiatric disorder appears to result from ischaemic damage caused by vasculitis [7], but the role of anti-Ro/SS-A alone ... American Psychiatric Association (APA): Diagnostic and Statistical Manual of Mental Disorders, 4th edition, text revision Washington, DC, USA: American Psychiatric Association Press 2000 10 Esch T:...

Ngày tải lên: 08/08/2014, 23:21

... primary Sjögren s syndrome [57] No statistically significant association can be ascribed to tumour necrosis factor-α and primary Sjögren s syndrome [58] The contribution of Ro/SSA and La/SSB in Sjögren s ... In complex diseases, one specific gene is neither necessary nor sufficient for disease expression This makes the genetics behind these diseases more complicated than those of diseases with a simple ... gene associations [37] In Caucasians of northern and western European backgrounds, including North American Caucasians, Sjögren s syndrome is among several autoimmune dis- eases that are associated...

Ngày tải lên: 09/08/2014, 01:21

... discussed is based on genes within the Aec2 locus, important in the homeostasis of lipids, lipoproteins, cholesterols, and fatty acids Secondary effects of impaired lipid homeostasis could result ... investigations of polymorphism in, or close to, candidate genes from susceptible and resistant mouse strains, speculations on disease mechanisms has to be considered preliminary The re-defined Aec2 locus ... Stia1 (serum transfer induced arthritis 1); Mbis1 (mycobacterium-induced systemic lupus erythematosus 1) This might suggest shared disease pathways in different models for autoimmune diseases...

Ngày tải lên: 09/08/2014, 01:22

... antibodies directed against La-antigen; hLFS, lymphocytic focus score ≥ 1.0 in sublabial minor salivary glands biopsy DM, dermatomyositis; Pt, patient; SSc, systemic sclerosis; SLE, systemic lupus erythematosus ... with SjS and slightly more disease specific than the LFS [13,14] The comorbidity of the six patients with secondary SjS is shown in Table Secondary SjS was accompanied by SLE in four patients, ... SjS-specific autoantibody showing a better disease sensitivity and specificity profile Haneji and colleagues suggested the presence of anti-αfodrin autoantibodies as a highly specific diagnostic...

Ngày tải lên: 09/08/2014, 01:23

... American-European Consensus Group (AECG) criteria for Sjögren' s syndrome with Statistical comparison offocus scores (FSs) baseline and cumulative focus scores (FSs) Receiver operating characteristic ... sectioning and assessment of a cumulative focus score (cFS) changes the baseline classification in 6% of patients evaluated for SS and increases the diagnostic performance of the criteria recently ... 0.016) (Fig 1) Sensitivity and specificity did not change for biopsies with FS = or FS < (inconsistent with SS), while specificity increased substantially in biopsies consistent with SS (FS ≥ 1) (Table...

Ngày tải lên: 09/08/2014, 06:22

... Amplification primers for the human genes were as follows: BAFF, 5'-TGAAACACCAACTATACAAAAAG-3' and 5'-TCAATTCATCCCCAAAGACAT3'; β-actin, 5'-GCTGTGCTACGTCGCCCT-3' and 5'-AAGGTAGTTTCGTGGATGCC-3' Primers ... patients with pSS in comparison with controls [31] Our results add to the understanding of the pathogenic involvement of SGECs in pSS Such cells can not only express and present autoantigens but can ... Vitali C, Bombardieri S, Jonsson R, Moutsopoulos HM, Alexander EL, Carsons SE, Daniels TE, Fox PC, Fox RI, Kassan SS, et al.: Classification criteria for Sjogren 's syndrome: a revised version of...

Ngày tải lên: 09/08/2014, 07:20

... statistics The Wilcoxon signed rank test was used to assess the statistical significance of changes in the subjective efficacy measures (VAS scores), tender points, tender joints, ESR, CRP, B cells, ... cells, T cells, and Igs, compared with their baseline values All statistical tests used a significance level of ≤0.05 Results Demographics and baseline disease characteristics Sixteen patients ... low-level positive HAHA are of uncertain clinical significance because they were not associated with specific clinical signs and symptoms or other apparent toxicities Pharmacokinetics Serum samples for...

Ngày tải lên: 09/08/2014, 08:22

... manuscript Acknowledgements We acknowledge Eva Mickholz for technical assistance CM is supported by grants from the Deutsche Forschungsgemeinschaft (DFG; MA 2247/2-1) References Kassan SS, Thomas ... ELISA technique Statistics Data were analysed with the statistical software package SPSS 12.0 Nonparametric tests were used for statistical analysis because a normal distribution of values could ... Germany) as described previously [17] Activated CD4+ T cells (CD4/CD25, CD4/CD45RO, CD4/CD69, CD4/ CD71), activated CD19+ B cells (CD19/CD86) and activated CD14+ monocytes (CD14/HLA-DR) were detected...

Ngày tải lên: 09/08/2014, 10:20

... most substantive difference between pSS patients and control individuals was the secretomotor subscale scores, as expected There was also evidence of bladder dysfunction, as we Table COMPASS scores ... testing, and more severe fatigue COMPASS scores Self-reported autonomic symptoms, as assessed using the COMPASS score (Table 2), were increased in pSS patient relative to control individuals ... primary Sjögren' s syndrome (pSS) The highest loadings for factor were observed with both the secretomotor and pupillomotor subscales (b) Scatterplot of the COMPASS factor scores for each pSS patient...

Ngày tải lên: 09/08/2014, 10:23