site specific incorporation of extra components into rna by transcription using unnatural base pair systems
Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
Ngày tải lên :
17/03/2014, 10:20
... parameters using F12 medium We found that tubulin Ó FEBS 2002 Incorporation of nitrotyrosine into a-tubulin (Eur J Biochem 269) 5043 Fig Reversible incorporation of nitrotyrosine into tubulin ... incorporation of nitrotyrosine into tubulin of cultured A549 cells led to decreased length of microtubules, and increased perinuclear localization and aggregation with consequent alteration of ... substitution of C-terminal tyrosine of a-tubulin by 3nitrotyrosine alters the ability of the molecule to form microtubules For this purpose, we studied the behavior of nitrotyrosinated tubulin (and of...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
Ngày tải lên :
20/06/2014, 00:20
... times RNA was eluted off the column using 30 μl of RNase free water The yield of extracted total RNA was determined by meas- uring absorbance at 260 nm, and purity was assessed using the ratio of ... expression analysis RNA extraction Total RNA was extracted using the Trispin method[37] Explants were disrupted in liquid nitrogen utilizing a tissue crusher, homogenized in ml of TRIzol (Invitrogen, ... at 260 nm to 280 nm The average RNA yield was approximately μg of total RNA per sample Real Time RT-PCR Reverse transcription Reverse transcription was performed using Stratascript™ reverse transcriptase...
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báo cáo khoa học: " Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo" pdf
Ngày tải lên :
11/08/2014, 00:22
... conjugation of QD's to Akt-PH-EGFP via intein mediated protein splicing (a) Schematic representation of site- specific intein-mediated conjugation of QD's to target protein (b) Co-localization of QDot585 ... under the GFP filter, in lysates of Xenopus embryos injected with RNA only or RNA and probe, but not QD's only Page of (page number not for citation purposes) Journal of Nanobiotechnology 2009, 7:9 ... pCS2++ between the EcoRI-XhoI restriction sites All plasmids were transcribed into RNA using mMessage mMachine Sp6 kit (Ambion) and the mRNAs were purified using the Mega Clear kit (Ambion) Microinjections...
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báo cáo khoa học: "Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo" pptx
Ngày tải lên :
11/08/2014, 00:23
... of QDs to the C-terminus of the PH domain of Akt (B) Schematic representation of site specific Ssp DnaB mini intein-mediated conjugation of QDs to the N-terminus of mem-EGFP homology domain of ... N-terminus of target proteins This expands the possibilities of the intein-based system allowing for the first time in vivo site specific conjugation of QDs and other nanostructures to the N terminus of ... to those of biological macromolecules (iv) lack of methodologies for the efficient delivery of QDs into cells [9,10] Although some of the above issues are gradually being resolved, site specific...
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Báo cáo y học: "Vpu-dependent block to incorporation of GaLV Env into lentiviral vectors" pot
Ngày tải lên :
12/08/2014, 23:22
... lack of effect of R8.91 on intracellular GaLV TM levels, analysis of vector particles harvested from culture supernatants revealed a further Page of 12 defect in the incorporation of GaLV Env into ... understand the effect of R8.2 on GaLV Env, we investigated the fate of both the Pr85 and SU proteins in the presence of lentiviral vector components using a labeled version of the protein containing ... to incorporation of the Env protein from feline endogenous virus RD114 into lentiviral vectors based on simian immunodeficiency virus (SIV) [38], which was suggested to be caused by a lack of...
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A site specific design of a fixed pitch fixed speed wind turbine blade with multiple airfoils as design variable
Ngày tải lên :
09/09/2015, 10:17
... for the extrapolation of the coefficients of lift and drag from -180 to 180 degrees by utilizing the AERODAS model as designed by Spera [24] An example of an extrapolation of the coefficients is ... Considering that the most of the energy gathered from the wind profile in the area of Roswell are at wind speeds of 10m/s as shown in Figure an analysis of the Reynolds number in the baseline blade will ... An estimation of the Reynolds number in the blade’s mid-section of the baseline Phase VI blade which has a chord length of 0.54m needs to be established in order to set a baseline of analysis Considering...
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Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
Ngày tải lên :
07/03/2014, 15:20
... by hydrophobic chromatography increased the specific activity of AC eluted with 0.1 M NaCl by a factor of 1.3 (from 138 UÆmg)1 to 185 UÆmg)1) and the percentage of CaM activation by a factor of ... residues of the catalytic site, favors a new activation mechanism Recently, the resolution of the 3D structure of the AC exotoxin of B anthracis and of its complex with CaM led to the proposal of a ... Fluorescence anisotropy decay parameters of the Trp emission of AC and AC–CaM complex obtained by MEM analysis of the fluorescence polarized decays, using a 1D model of the anisotropy where all lifetimes...
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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
Ngày tải lên :
18/06/2014, 16:20
... CEA mRNA score [(CEA mRNA/ GAPDH mRNA) × 106] of the 123 patients was 37,510.0 (range, 0-3,695,652.1) copies Figure showed the distribution of (CEA mRNA/GAPDH mRNA) × 106 in this group of patients ... from literature for detection of tumor cells by real-time RT-PCR of mRNA markers It shows the positive rate of mRNA markers from literature for detection of tumor cells by real-time RT-PCR Abbreviations ... often performed based on CEA mRNA positivity, which is determined using a cutoff level [13] CEA mRNA can be detected in patients with benign disease as well as healthy volunteers, so the cutoff...
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In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx
Ngày tải lên :
10/08/2014, 05:21
... expression of TNF-α was not affected by IL-10 shRNA treatment (Fig 3B) The suppression of IL-10 by IL-10 shRNA was dose-dependent (Fig 3C) Figure 3(D) shows the suppression of IL-10 by IL-10 shRNA after ... Suppression of IL-10 expression by IL-10 shRNA in RAW 264.7 cells Two preparations of IL-10 shRNA (IL-10i-1 and IL-10i-2) were packaged into JCV VLPs by osmotic shock and were confirmed by PCR (Fig ... Effects of VLPs IL-10 shRNA on morphology and viability of RAW 264.7 cells (A) Two preparations of IL-10 shRNA (IL-10i-1 and IL-10i2) were packaged into JCV VLPs by osmotic shock The presence of IL-10...
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Báo cáo y học: "Highly specific inhibition of leukaemia virus membrane fusion by interaction of peptide antagonists with a conserved region of the coiled coil of envelope" potx
Ngày tải lên :
13/08/2014, 05:21
... of HTLV-1 and BLV TM (Figure 1B) Using this approach, we observed that for the core coiled-coil the majority of conserved residues map along the grooves formed by the interface of each pair of ... coil of TM and, in a trans-dominant negative manner, blocks resolution of the pre-hairpin intermediate to the trimer -of- hairpins, thus impairing the fusogenic activity of TM The potency of these ... and specificity of LHR-based synthetic peptides from HTLV-1 and BLV a peptide based on the LHR of BLV was generated The synthetic peptide designated PBLV-391 includes residues Cys391 to Gln419 of...
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Báo cáo sinh học: "Marginal maximum likelihood estimation of variance components in Poisson mixed models using Laplacian integration" ppt
Ngày tải lên :
14/08/2014, 19:22
... likelihood estimation of variance components in a Poisson mixed model based on Laplace’s method of integration, as suggested by Leonard (1982) for calculating posterior modes, and by Tierney and Kadane ... are both a function of or2 U marginal distribution of Jfl(or maximum by recourse to Laplacian integration, as in Whe wish to find the mode of the of the marginal likelihood of the data) Leonard ... is computed from the mode of the joint posterior density of } a p and u, given Q One can find the posterior marginal mode of Jfl by establishing u a grid of points of {Qu, LA(Q! ! y)} and then...
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Removal of arsenic from synthetic groundwater by adsorption using the combination of laterite and ironmodified activated carbon
Ngày tải lên :
05/09/2013, 09:38
... stock solutions of As(III) were prepared from As(III) standard solution of 1003mg/L (Wako Pure Chemicals Ltd, Japan) The stock solutions of As(V) were prepared by dissolving 4.1646mg of Na2HAsO4.7H2O ... (Wako Pure Chemicals Ltd, Japan) into distilled water with a total volume of 1000mL This stock solution has an arsenic concentration of 1000mg/L - 44 - Journal of Water and Environment Technology, ... preparation of iron-modified activated carbon (AC-Fe) was conducted by impregnation with 5% of iron onto AC (theoretical calculation based on weight) The impregnation method for preparation of AC-Fe...
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Removal of arsenic from simulated groundwater by adsorption using iron-modified rice husk carbon
Ngày tải lên :
05/09/2013, 09:38
... removal using iron-based adsorbent In this study, the surface of the rice husk carbon is modified by impregnation with iron Moreover, most researches have been done using pure water instead of actual ... investigate the effects of pH values within the range of 5-9, of initial arsenic concentrations within the range of 1.42 - 7.39 mg/L and of adsorbent doses within the range of 0.5 - 5.0g/L, as well ... RH-Fe5%, particle size of 0.1-0.35 mm, with Co of 1.42-7.38 mg/L, adsorbent dose of 2.5 g/L, pH of simulated groundwater (pH 8.18) Comparison of adsorption isotherms The values of the linear and nonlinear...
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Báo cáo sinh học: " Generation of high-titer viral preparations by concentration using successive rounds of ultracentrifugation" doc
Ngày tải lên :
18/06/2014, 22:20
... Assessment by flow cytometry of transduction by retrovirus following concentration using different numbers of rounds of centrifugation μL of retrovirus was added for each transduction B Titration of ... μg of DNA (28.26 μg of the H1 shRNA hairpin vector; 18.3 μg of pMDLg/ pRRE; 9.86 μg of pMD2.G and 7.04 μg of pRSV Rev) and 229.4 μL of M Calcium solution in a total volume of 3.7 mL The transfection ... number of rounds of centrifugation C Addition of an addition round of centrifugation without addition of unconcentrated supernatant does not result in a decrease in viral titre D Demonstration by...
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báo cáo hóa học:" Generation of high-titer viral preparations by concentration using successive rounds of ultracentrifugation" pot
Ngày tải lên :
20/06/2014, 04:20
... Assessment by flow cytometry of transduction by retrovirus following concentration using different numbers of rounds of centrifugation μL of retrovirus was added for each transduction B Titration of ... μg of DNA (28.26 μg of the H1 shRNA hairpin vector; 18.3 μg of pMDLg/ pRRE; 9.86 μg of pMD2.G and 7.04 μg of pRSV Rev) and 229.4 μL of M Calcium solution in a total volume of 3.7 mL The transfection ... number of rounds of centrifugation C Addition of an addition round of centrifugation without addition of unconcentrated supernatant does not result in a decrease in viral titre D Demonstration by...
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Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt
Ngày tải lên :
16/03/2014, 16:20
... incorporation into lipids was linear over a period of h under the conditions used Incorporation of [3H]glycerol into triacylglycerol was determined using a modification of the method described by Davis ... lipids were extracted and separated by TLC, as described previously [22] Proteins were harvested from the plates, after extraction of the lipids, by the addition of mL of NaOH (1 M) mRNA analysis ... mechanism of this effect by comparing the influence of CRLPs and CRLPs containing probucol (pCRLPs) on the uptake of the particles by the cells, intracellular lipid synthesis, and the expression of mRNA...
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Báo cáo khoa học: Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins docx
Ngày tải lên :
16/03/2014, 22:20
... Semiquantitative assessment of the incorporation level of DOPA was made by comparison of the sum of the peak heights of the 3-DOPA species (peaks E, G, H and I) vs the sum of the peak heights of the 2-DOPA ... digestion of His6-PpiB that had been produced using mm tyrosine or mm DOPA were separated by HPLC and 3165 Translational incorporation of DOPA into proteins analysed by in-line ESI-MS Peptides of Mr ... (2004) Incorporation of non-natural amino acids into proteins Annu Rev Biochem 73, 147–176 Budisa N (2003) Expression of ‘tailor-made’ proteins via incorporation of synthetic amino acids by using...
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Báo cáo khoa học: "Unsupervised Decomposition of a Document into Authorial Components" pdf
Ngày tải lên :
17/03/2014, 00:20
... One of the advantages of using biblical literature is the availability of a great deal of manual annotation In particular, we are able to identify synsets by exploiting the availability of the ... documents can be decomposed into authorial components with very high accuracy by using a two-stage process First, we establish a reliable partial clustering of units by using synonym choice and ... to opposite classes) 7.3 Empirical Results We randomly generated composite books for each of the book pairs considered above In Figures and 4, we show for each book pair the percentage of all...
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Báo cáo khoa học: Dissection of LolB function – lipoprotein binding, membrane targeting and incorporation of lipoproteins into lipid bilayers potx
Ngày tải lên :
23/03/2014, 05:22
... presence of the indicated concentrations of arabinose by inoculation of portions of the cultures into fresh medium (B) The same amounts of cells grown in the presence of various concentrations of arabinose ... phospholipid The mLolB-dependent incorporation of Pal into liposomes containing various combinations of phospholipid was examined at 30 °C for 10 (Fig 6B) The incorporation of Pal into liposomes prepared ... membrane incorporation of lipoproteins However, as mLolB was found to be able to catalyse the incorporation of lipoproteins, even into liposomes, no extra factor is required for the final step of lipoprotein...
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Báo cáo khoa học: Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail (Coturnix japonica) doc
Ngày tải lên :
23/03/2014, 07:20
... successfully incorporate into the PL Results Incorporation of exogenously injected ZP1 into quail PL To determine whether ZP1 injected intravenously into a bird was incorporated into the PL of the ovarian ... 3583 Incorporation of ZP1 into perivitelline membrane M Kinoshita et al Incorporation of heterologous ZP1 into quail PL 200 kDa 117 kDa 97 kDa 66 kDa 45 kDa 31 kDa Fig Ligand blot analysis of PL ... might be responsible for the incorporation of serum ZP1 into the PL Species-specific interaction of quail ZP3 with ZP1 from various species In our labeled ium of of the previous study we demonstrated...
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