single nucleotide polymorphism microarray analysis of genetic alterations in cancer

... Oilseed maintainer Oilseed maintainer Oilseed maintainer Non-oilseed maintainer Oilseed maintainer Oilseed maintainer Oilseed maintainer Oilseed maintainer Oilseed maintainer Oilseed maintainer Oilseed ... Klein × Local (a pool of local varieties of INTA Pergamino breeding program including "Guayacán INTA", "Charata INTA") × "Manfredi" (a pool of varieties from INTA Manfredi breeding program including ... genetics via LD mapping is an emerging field of genetic mapping that has the potential to reach resolution to the level of individual genes (alleles) underlying quantitative traits A Single Nucleotide...

Ngày tải lên: 12/08/2014, 05:20

... Schematic illustration of the different steps involved in genetic analysis of single cells from tissue sections 37 Detection and analysis of genetic alterations in normal skin and skin tumours Applications ... and analysis of genetic alterations in normal skin and skin tumours containing an inherent p53 mutation affecting different alleles, were sequenced Sequencing was carried out using dye terminators ... resulting in a more intricate, and thus more easily influenced folding of the strands (Highsmith, 1999) Detection and analysis of genetic alterations in normal skin and skin tumours The detection of...

Ngày tải lên: 10/04/2014, 22:12

... with inbreeding coefficients in both populations and interpopulations 30 Analysis of Genetic Variation in Animals The inbreeding coefficient of the whole population (FIT = Factor of Inbreeding in ... analysis is done by individual genetic profiling (genetic fingerprinting), determining the interrelations between different individuals and evaluating their allele frequency in populations Microsatellite ... Characterization of 37 breed-specific single- nucleotide polymorphisms in sheep Journal of Heredity 97: 531 Pariset, L et al 2006b Characterization of single nucleotide polymorphisms in sheep and...

Ngày tải lên: 28/06/2014, 08:20

... purine metabolism (0.0042) L carbohydrate metabolism (0.00073) M Coexpression Lehner genetic interaction Protein-protein interaction Query interaction Fine genetic interaction SGI genetic interaction ... linked by seven SGI interactions to the bar-1 query gene, 11 fine genetic interactions, 36 co-phenotype links, three coexpression links, and one protein-protein interaction link To further investigate ... co-classified interacting pairs in V)/(number of possible co-classified pairs) and Precision (V) = (number of co-classified interacting pairs in V)/(number of interacting pairs in V) We estimated...

Ngày tải lên: 06/08/2014, 18:21

... galactoside-binding, soluble, binding protein 3.8 Up IL1RN Interleukin-1 receptor antagonist 3.5 Up APOBEC3B Phorbolin 1-like 2.0 Up RGS1 Regulator of G-protein signaling 1.8 Up AGRN Agrin > 71.2 ... among the IFN-induced genes overexpressed in the JDM muscle samples Increased expression of several genes, including G1P3 (encoding IFN-induced 6-16) and CDKN1A (p21 cyclin kinase inhibitor), were ... genes in data derived from multiple laboratories using distinct microarrays raises the profile of IFN as a pathogenic mediator of the myriad alterations to the immune system seen in SLE In view of...

Ngày tải lên: 09/08/2014, 01:23

... An increase in PAI-1 suppresses the generation of plasmin resulting in hypofibrinolysis and a relative hypercoagulable state [1] Decreased fibrinolytic activity, which may be a consequence of increased ... NM_031590 WNT1 inducible signaling pathway protein 0.0105 + 1.67 NM_012587 integrin binding sialoprotein 0.0276 + 1.66 NM_053816 calcitonin receptor 0.0316 + 1.63 NM_013191 S100 protein, beta polypeptide, ... viable cells expressing these molecules in comparison to G2 Differences could also be explained in that gene expression analysis findings are supportive of a result effect indicating steroid treatment...

Ngày tải lên: 12/08/2014, 14:22

... direct labeling of microRNAs using radioactivity instead of a glass microarray and RT-PCR/fluorescent labeling, as we used in our study Despite differences in sample selection as Whole brains from ... a free amino group linked to its 5' terminus through a 6-carbon spacer (IDT) and was printed onto amine-binding slides (CodeLink, Amersham Biosciences) Control probes contained two internal mismatches ... than twofold are shown in five columns corresponding to the five time points Colors indicate relative signal intensities The microRNA expression profile was sorted using a hierarchical clustering...

Ngày tải lên: 14/08/2014, 14:21

... important roles in interaction with its host Point mutations in the putative calcium-binding site (CBS) or hinge connecting shell and protruding domains of TCV CP appear to alter virus-ion interactions, ... 106 against virion antibody at X 105 in detection of TCV in infected N benthamiana Lanes to 10 contained equal loading of total protein of N benthamiana M: BenchMark® Pre-tained Protein Ladder ... affecting interactions between CP and plant hosts (Lin and Heaton, 1999) Moreover, Nterminus of the TCV CP was shown to be involved in eliciting resistant responses in Di-17 Arabidopsis, suggesting...

Ngày tải lên: 26/11/2015, 12:14

... any mixed gain-loss pairs We also detected the trisomy of 1q [21], which appeared in all tumor categories in the associations involving gain of chromosome (Figure 2a; Figure S2a-d in Additional ... involved only in expected links (for example, a link between a translocation and a gain/loss of one of its derivative chromosomes; a link between two (two-break) translocations originating from one ... and are often used in verifying the existence of specific aberrations We emphasize that in our analysis we analyzed all types of aberrations identifiable by cytogentic techniques, including translocations,...

Ngày tải lên: 09/08/2014, 23:20

... the proportions of positive and negative studies analysing a determined polymorphisms between the studies examining it within 1-3 polymorphisms versus studies examining the same polymorphism among ... the usefulness of certain polymorphisms as prognostic genetic markers It may also have direct impact in medical research by guiding researchers and funding sources in investigating insignificant ... reasons the number of genetic association studies is increasing rapidly and this trend is expected to accelerate due to the availability of mapped single- nucleotide polymorphisms (SNP) in the human...

Ngày tải lên: 25/10/2012, 10:51

... occur in carriers of the risk allele at later stages of the disease An interesting finding of the study presented here is the disproportionately high frequency of the PTPN22 1858T allele in male ... increased frequencies in RA patients The CT and TT genotype was present in 37.9% of patients and 19.2% of healthy controls, resulting in an OR of 2.57 (95% CI 1.85–3.58, p < 0.001) Carriage of ... participated in the design of the study, statistical analysis, interpretation of the results, and writing of the final manuscript Acknowledgements We are grateful to the patients with RA, the control individuals,...

Ngày tải lên: 09/08/2014, 08:22

... causing the haplotype association Homozygosity could explain the genetic risk associated with rs729749 The rs726749 SNP is noncoding and located in the beginning of intron in NCF4 Analysis of ... based on findings in animal models The Pia4 locus in rats has been shown to reduce the severity of pristane-induced arthritis (an arthritis model) [20] A single nucleotide polymorphism (SNP) in the ... arguments in favour of subgrouping RA patients, and an analysis of nonstratified samples might conceal a true association In the initial analysis of the genotype frequencies we found indications of...

Ngày tải lên: 09/08/2014, 10:21

... Medical School of Nanjing University, China The controls had no symptoms or histories of CDH All subjects included in the study were of Han Chinese origin living in and around Nanjing No subjects ... a key role in the aetiology and pathogenesis of CDH To evaluate this possible association, we examined the genetic association of the GDF5 SNP with CDH in people of Han Chinese origin and found ... GH, Zhang LJ, Ji SJ: [Association analysis on the polymorphisms of PCOL2 and Sp1 binding sites of COL1A1 gene and the congenital dislocation of the hip in Chinese population] Zhonghua Yi Xue...

Ngày tải lên: 09/08/2014, 13:22

... epithelium of the ileum and colon with differentiated cells being sloughed off into intestinal lumen staining most vividly (Figure 1A,1B,1C) Examination of the intracellular staining pattern ... epithelium of the terminal ileum with cytoplasmic staining of the terminal web and lateral junctions of the epithelial cells (green arrow) B Staining of the superficial epithelium of the colon C Staining ... it does in macrophages The most intense cytoplasmic staining occurred in the terminal web of the epithelial cell and in the lateral junctions of the cells The localization of CSF1R in actin-rich...

Ngày tải lên: 11/08/2014, 10:23

... sequence (CDS) within 70 bp of an exon-intron junction Genes were grouped into bins of nine equal intervals according to the proportion of sequence within 70 bp of an exon-intron junction, and ... We obtained human protein-protein interaction data from the IntAct database [64] and examined how SNP A/S ratios are correlated with the connectivity of proteins in the protein-protein interaction ... SCAN domain PF00059 0.43 58 Lectin C-type domain PF07859 0.42 21 alpha/beta Hydrolase fold PF00048 0.40 23 Small cytokines (intecrine/chemokine), interleukin-8 like PF00105 0.14 38 Zinc finger,...

Ngày tải lên: 14/08/2014, 08:21

... coding regions Most of them (414) cause a frame-shift in the reading frame of the associated gene, indels lead to single amino acid deletions and were single amino acid insertions Comparison of ... 6,292 SNPs in untranslated regions Additionally, 203 indels were located in coding regions, with almost all of them (201) causing a frame-shift in the corresponding gene The remaining two indels ... Abbreviations Indel: small insertion/deletion event; MAF: minor allele frequency; MALDI-TOF: matrix-assisted laser desorption/ionization time -of- flight; SNP: single nucleotide polymorphism Analysis software...

Ngày tải lên: 14/08/2014, 21:21

... appear unchanged in the presence of the inhibitor (–) Thus, a decrease in the expression of MMP-1 in the presence of PD098059 may be due, at least in part, to a decrease in the binding of transcription ... 1G SNP Although increasing concentrations of PD098059 reduce the binding of complexes to the probe containing the 1G SNP, a more pronounced decrease is observed in the binding of complexes to ... h (Fig 4) The increase in MMP-1 mRNA levels in untreated cultures beginning at h has been observed previously in response to removal of serumcontaining medium [57] This increase in MMP-1 mRNA...

Ngày tải lên: 21/02/2014, 00:20

... strain Thai0067 had a homologous substitution of isoleucine to valine at pos 110 of the deduced sequence of the GnGc protein This suggested a strong stabilising selection operating on the protein ... RT-PCR and sequencing, participated also in the genetic analysis and drafting the manuscript VH participated in the study design, trapping and screening of rodents, and drafting the manuscript ... in the study coordination and trapping and screening of rodents HH participated in the study design and coordination and drafting the manuscript AP participated in the study design and coordination,...

Ngày tải lên: 20/06/2014, 01:20

... mammalian Sin3 co-repressor protein [10,11] The effectiveness of the Sin3 binding domain of Mad in silencing gene expression when fused to DNA binding proteins has been demonstrated in two studies ... DNA binding domain and demonstrated specific inhibition of transcription mediated by binding to AP-1 binding site elements [12] Another group of investigators fused Mad SID to the tetracycline ... follicles in sheep [32] Serpine2 protein levels are elevated in dominant bovine follicles [33], whereas the levels of Serpine2 are lower in ovaries of Foxl2 knockout mice suggesting that the gene is induced...

Ngày tải lên: 20/06/2014, 07:20

... whole joints, but not from a single species of cell, we could not detect the change of gene expression in a single species of cell when the content of this single species of cell was low in the ... expression of serum amyloid proteins, metalloproteinases, chemokines, and cytokines is frequently seen in the joints of patients with RA [27,29], indicating that the gene expression profiles obtained ... from joints After removal of the skin and muscle, portions of the leg containing the knee, ankle, and finger joints were rapidly frozen in liquid nitrogen Frozen joints were homogenized using a...

Ngày tải lên: 09/08/2014, 08:22