single cell cultivation chip system

... other macrophages [89,108,76,109], nerve cells [110] and other cells[111] are available This is an expected phenomenon for phagocytic cells (macrophages) and cells that function as a barrier and/or ... endothelial cells J Cell Sci 1988, 91:231-238 Morgan DM, Larvin VL, Pearson JD: Biochemical characterisation of polycation-induced cytotoxicity to human vascular endothelial cells J Cell Sci 1989, ... association with the cell membranes after application to human skin [91] The authors suggested that single molecules, which make up the particles, may penetrate the intercellular spaces and,...

Ngày tải lên: 11/08/2014, 00:22

... [27] Cultures (5·104 cells/well) of P-ECs and of PA-SMCs were prepared, and IL-6 levels in the culture cell lysates were measured using an ELISA (R&D Systems, Lille, France) Cells were used for ... receptors are expressed not only by inflammatory cells, but also by constitutive vesselwall cells Thus, both pulmonary vessel cells and inflammatory cells may be targets for IL-6 Since the hypoxic ... performed in duplicate using the ABI Prism 7000 system (Applied Biosystems Foster City, CA) PCR primers were designed using Primer Express Software (Applied Biosystems) To avoid inappropriate amplification...

Ngày tải lên: 12/08/2014, 14:20

... at a bead to cell ratio of 20:1 Non-adherent cells were collected after the removal of free and cell bound beads using a magnetic bead concentrator (Dynal, MPC 6) The resulting cell populations ... presenting cells to become activated antigen presenting cells, which then migrate to draining lymph nodes to stimulate cellular immune responses [34] The migra- tion of activated antigen presenting cells ... distribution of two main candidate antigen presenting cell types: namely, B lymphocytes (B220 positive cells) and dendritic cells (CD11c positive cells [37,38]) As shown in Figure 5, control, LTβ-KO,...

Ngày tải lên: 03/11/2012, 11:24

... of TLRs in lung cells or tissue TLRs are widely expressed on both resident lung cells as well as infiltrating cells of myeloid and lymphoid origin Primary bronchial epithelial cells express mRNA ... mRNA [35] Finally, lung tissue cells may also be activated through cooperative interactions with TLR responsive lymphoid cells, as exemplified by airway smooth muscle cell activation via IL-1b release ... IL-10 [184] while basophils may also instruct T cells to become Th2 cells [185] TLRs have been shown via genetic association studies as well as single and multiple gene knockout studies to play...

Ngày tải lên: 11/08/2014, 03:20

Ngày tải lên: 11/08/2014, 03:21

Ngày tải lên: 11/08/2014, 07:20

... differential cell counts of the bronchoalveolar lavage fluid (BALF) The total cell count was measured by using a hemocytometer The differential cell count was determined by manually counting 200 cells ... fibroblast proliferation and synthesis of extracellular matrix, occur [9] Various types of cells, including macrophages and neutrophils have been the immune cells primarily implicated as playing potential ... cells and neutrophils were barely detected as infiltrating cells in the lung (data not shown) Discussion The ability of CD69 to function as a signal transducing molecule in various types of cells,...

Ngày tải lên: 12/08/2014, 14:20

... location of ED-1 positive cells was determined with immuno-electron microscopy, we counted these cells in lung sections under a light microscope BDL rats showed more ED-1 positive cells in their alveolar ... Quantification of ED-1 positive cells The methods have been described previously [15] An observer, who was blinded to the identity of treatment groups, counted the ED-1 positive cells in alveolar septa ... sections from control rats (C) contained occasional septal ED1-positive cells (arrow) while those from BDL rats had numerous reactive cells in the septa (arrows) Immunoelectron micrograph (E) shows gold...

Ngày tải lên: 12/08/2014, 14:20

... signal intensity under different experimental conditions (d) shows a single bronchiole from a non-sensitized mouse, (e) A single bronchiole from a sensitized mouse treated with control mismatched ... inflammation (Figure 3e) with goblet cell hyperplasia, compared with the non-sensitized animals No differences were found in the total number of inflammatory cells or in eosinophil accumulation ... since in vitro and ex vivo data suggest that this PG contributes to migration of mast cells and dendritic cells and, therefore, promotes inflammation [49,50] However, we believe that a more complex...

Ngày tải lên: 12/08/2014, 14:20

... inflammatory cells as that caused by ATP is believed to act in the intercellular signal transduction as a "purinergic system" in multiple organs, and is involved as an energy source in cellular metabolism ... subtypes in different systems Int Rev Cytol 2004, 240:31-304 Guyot A, Hanrahan JW: ATP release from human airway epithelial cells studied using a capillary cell culture system J Physiol 2002, ... at 4°C, the cell pellets were resuspended in ml of saline The total number of cells in BAL fluid was counted with a hemocytometer Cytospins were prepared from resuspended BAL fliud cells, using...

Ngày tải lên: 12/08/2014, 14:20

... elevation of intracellular cAMP has been associated with the general suppression of activity of inflammatory cells and relaxation of airway and vascular smooth muscle [17] Levels of intracellular cAMP ... methylcellulose) or Cytokines and chemokines quantification ELISA kits for human IL-8 (BD Biosciences), human monocyte chemoattractant protein-1 (MCP-1; R&D Systems), and murine KC (R&D Systems) ... production by human broncho-epithelial cells in vitro We next evaluated the effect of combined 4B/4D and 7A AONs on the functional activity of NHBE cells in vitro Cells were transfected with a combination...

Ngày tải lên: 12/08/2014, 14:20

... the detection limit and we report only the IL8 results For logistic reasons, we did not determine cellular composition Exhaled Nitric Oxide Levels The levels of FeNO were measured following the ... continuous analyzer (CDL 88 sq Michigan, USA) The FeNO reading was displayed in the monitoring system and the mean of the three acceptable tests was taken Exhaled Breath Condensate Collection ... days of the study period The home of each child was geo-referenced using a geographic information system and the closest monitoring station was assigned to the child All children attended public...

Ngày tải lên: 12/08/2014, 14:20

... been detected in mouse lung endothelial cells, mouse RAW264.7 cells, rat pulmonary microvascular endothelial cells and rat pulmonary artery endothelial cells [15] High TLR9 expression levels have ... expressed in all cells present in the lung For instance, TLR9 is absent in rat pulmonary arterial smooth muscle cells [15], mouse pulmonary macrophages [46] and in lung dendritic cells [47] This ... * charge of the bronchoalveolar lavage (BAL) and cell counts All authors read and approved the final manuscript TLR9-D + CpG-ODN cell count (cells/µL) Acknowledgements 2000 This work was supported...

Ngày tải lên: 12/08/2014, 15:21

... CD8+ T cells are critical for clearance of respiratory viruses, but depending on the number of CD8+ T cells and overall immune status, these cells may cause severe lung pathology [42] CD8+ T cells ... Puig PE, Roux S, Parcellier A, Schmitt E, Solary E, Kroemer G, Martin F, Chauffert B, Zitvogel L: Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ ... PharMingen Cells were washed in and then resuspended in ml FACS buffer (PBS + 1% FCS) and counted Samples from influenza infected mice contained the most cells and were diluted to 250,000 cells for...

Ngày tải lên: 12/08/2014, 15:21

... (103 cells/ml) CD3 cells (%) CD3 cells (103 cells/ml) CD4 cells (%) CD4 cells (103 cells/ml) CD8 cells (%) CD8 cells (103 cells/ml) CD4/CD8 (ratio) B cells (%) B cells (103 cells/ml) NK cells ... Cellularity (104 cells/ml) Cell viability (%) AM (%) AM (104 cells/ml) Lymphocytes (%) Lymphocytes (104 cells/ml) Neutrophils (%) Neutrophils (104 cells/ml) Eosinophils (%) Eosinophils (104 cells/ml) ... cell pellet was washed and resuspended at 106 cells per ml The cell viability and differential cell counts were determined Total RNA and DNA were extracted from nasopharyngeal aspirates and cells...

Ngày tải lên: 12/08/2014, 18:21

... Therefore, we decided to undertake an in vivo single and multiple exposure study using rats to characterize cellular and molecular responses We hypothesized that single and multiple exposures to swine ... inflammation and a decline in lung function The data show that single and multiple exposures cause increase in AHR, inflammatory cells in BALF, mucus cells in the airways and lung inflammation Exposure ... http://respiratory-research.com/content/6/1/50 Figure Quantification of mucus producing cells in the airways Quantification of mucus producing cells in the airways Mucus producing goblet cells in the airways were quantified using PAS...

Ngày tải lên: 12/08/2014, 18:21

... like intercellular adhesion molecule (ICAM)-3 on the surface of apoptotic cells, amyloid peptid, ceramide, and urate crystals Ligation of CD14 by these ligands, except for apoptotic cells, mediates ... receptors both enhance LTAinduced cell activation [18] Presumably in a similar manner, CD14 also enhances TLR2-dependent cellular activation by LAM derived from the cell- wall of mycobacteria LAM ... [7] Likewise, sCD14 is able to mediate LPS-activation of cells with low membrane CD14 expression, such as epithelial and endothelial cells [9] However, at high concentrations, LBP and sCD14 are...

Ngày tải lên: 13/08/2014, 20:21

... consists of both cellular and non-cellular components The cellular components of the innate immune system include dendritic cells, monocytes, macrophages, granulocytes, and natural killer T cells, as ... times Cell pellet was resuspended in complete medium, a cell count was done and cell concentration was adjusted to × 106 cells/ml Feeder cells were used at a ratio of 2:1 i.e for every one T cell, ... antigen-presenting cell The most potent activators of naive T cells are mature dendritic cells and these are thought to initiate most T -cell responses in vivo Immature dendritic cells take up antigen...

Ngày tải lên: 03/10/2015, 21:57

... In the respiratory system most of the b-ARs are b2-ARs However, there are b1-ARs, too, which are responsible for the respiratory effects of cardioselective b1-antagonists Two systematic reviews ... hypertension: a systematic review J Hypertens 2004, 22(1):11-19 Chobanian AV, Bakris GL, Black HR, Cushman WC, Green LA, Izzo JL Jr, Jones DW, Materson BJ, Oparil S, Wright JT Jr, Roccella EJ: Seventh ... of high blood pressure and antihypertensive medication Thus, it allows only statements about a single point in time and does not allow evaluating the effect of long-standing high blood pressure...

Ngày tải lên: 25/10/2012, 10:45

... These clinical findings are associated with cellular defects, including hypersensitivity to killing and the mutagenic effects of UV and the inability of XP cells to repair UV-induced DNA damage [7] ... suggested to act on the single- stranded region created as a result of the combined action of the XPB helicase and the ERCC2/XPD helicase at the DNA damage site In human cells, ERCC5/XPG catalyses ... repair Mol Cell Biol 1995; 15: 5396-402 42 Sugasawa K, Ng JM, Masutani C, et al Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair Mol Cell 1998;...

Ngày tải lên: 31/10/2012, 14:59