... (MA7), and the oligo- nucleotides were subsequently annealed with their comple- mentary strands, and puried as above. UV Vis and CD spectroscopy CD spectra and melting curves of wild-type RepA and AEDANS-labeled, ... line) and AEDANS labeled (dash-dots), recorded at 23.5 °C with $ 2 l M protein and with intensities normalized with respect to their emission maximum at 327 nm. (D) Binding of wild-type RepA ( ) and ... for free dimeric RepA, and the complexes with 1IR, 1DR and 1DR-short were calculated in one of two ways: based on the 3D structure and based on a prolate ellipsoid shape with dimensions derived...
Ngày tải lên: 07/03/2014, 04:20
Ngày tải lên: 24/03/2014, 00:21
Tài liệu Creating Applications with Mozilla-Chapter 6. Packaging and Installing Applications-P3 pdf
... Macintosh The Macintosh BNDL resource (OS icons for files and applications) and related resource types (4bit, 8bit, large, small icons, masks, and FREF) are contained in nsMacBundle.rsrc, located ... the Mozilla application and do not want to hijack this resource completely. Icons can be installed as part of an XPI. file onto the local machine, and registers it with the chrome registry. ... initiate the installation of that XPI. As with any new software installation, however, a page that describes the package and what it does can help allay fears and promote use. 6.5. Extra Tricks...
Ngày tải lên: 14/12/2013, 12:15
Tài liệu Creating Cool Web Sites with HTML, XHTML and CSS (2010)- P7 pdf
... look at a site that’s a bit more complex: AnswerSquad. The subdirectory structure of AnswerSquad The AnswerSquad site— http://www.AnswerSquad.com/ —is more complex because it con tains a lot ... flyer-back .pdf flyer-front .pdf Please purchase PDF Split-Merge on www.verypdf.com to remove this watermark. 557386 Ch13.qxd 4/2/04 9:56 AM Page 304 Ł 304 Creating Cool Web Sites with HTML, XHTML, and ... purchase PDF Split-Merge on www.verypdf.com to remove this watermark. 557386 Ch13.qxd 4/2/04 9:56 AM Page 298 Ł 298 Creating Cool Web Sites with HTML, XHTML, and CSS Ł You can contract directly with...
Ngày tải lên: 24/12/2013, 04:15
Tài liệu The international community and the “NTP for coping with CC” pdf
... vulnerabilities, with national and international resources, public and private • Stress capacity building at all levels, and focus on the most vulnerable social groups, localities, and the most ... local, sectoral) strategies and plans for 2011 and beyond; in public investment decisions; and policy instruments such as SEA • Articulate VN policies, strategies and plans on vulnerability reduction ... Development; and in local action plans for 2011 and beyond. 7 2. What is needed, now and later? (d) Longer term action CCA (Phase 2) to focus on: • Implementation of specific projects (sectoral, and...
Ngày tải lên: 24/01/2014, 00:20
Tài liệu CONTROL OF ORGANIC COMPOUND EMISSIONS FROM CHEMICAL, COATINGS AND RUBBER PRODUCTS MANUFACTURING pdf
... inside and outside, with release compounds which help remove air from the tire during molding and prevent the tire from sticking to the mold after curing. (7) “Grinding mill” means any mill with ... all other valves, pumps, sealed agitators, compres- sors, and relief valves, and all flanges, once during each calendar year. 3. Notwithstanding subd. 1., if less than or equal to 2% of the valves ... chemical, coatings and rubber products manufacturing air contaminant sources and to their owners and operators. (2) PURPOSE. This chapter is adopted under ss. 285.11, 285.13, and 285.17, Stats.,...
Ngày tải lên: 14/02/2014, 08:20
Tài liệu Báo cáo khoa học: "Learning Parse and Translation Decisions From Examples With Rich Context" pdf
... system CONTEX with Magerman's SPATTER, and Collins' BLD; results for SPATTER, and BLD are for sentences of up to 40 words. Table 6 compares our results with SPATTER, and BLD. ... to-scale hand-crafted approach and the typically large-scale but context-poor statistical approach for unrestricted text parsing. Using • a rich and unified context with 205 features, • a complex ... a log file with the correct parse action sequence of training sentences as acquired under su- pervision and (2) a set of features, the system revis- its the training sentences and computes...
Ngày tải lên: 22/02/2014, 03:20
Beginning iPhone and iPad Web Apps: Scripting with HTML5, CSS3, and JavaScript pdf
... the command line with the chown command (CHange OWNership): sudo chown -R username /var/www Now, you can modify all files and directories on the web site without using the sudo command you ... Color Handling 140 Boxes and Borders 142 Box Sizing 142 Rounded Box Corners 143 Borders Drawn with Images 145 Shadows 147 Box Shadows 148 Text Shadows 149 Text Effect with Shadows and Outline ... App Design with HTML5 and CSS3 65 ■Chapter 4: The Anatomy of a Web Application 67 The iPhone Revolution 67 i Beginning iPhone and iPad Web Apps Scripting with HTML5, CSS3, and JavaScript...
Ngày tải lên: 06/03/2014, 07:20
Báo cáo khoa học: CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells pdf
... detectable in all samples, by contrast with cultures without NH 4 Cl. The most prominent bands were found in cell lysates of CpG-ODN-stimulated cells, with and without NH 4 Cl treatment. These data ... were detected with the monoclonal antibody 4H11. (B) An aliquot of RML- (lanes 1 and 3) or 22L- (lanes 2 and 4) infected brain homo- genate was analysed by immunoblot without (lanes 1 and 2) or after (lanes ... supernatant samples were split between those without and with PK digestion (20 lgÆmL )1 for 30 min at 37 °C). Digestion was stopped with Pefabloc and samples were subjected to detergent solubility...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... 4. CD spectra and differential spectra of DDI-1A and DDI-1B and their complexes with selected DNA sequences. Solid line, 20 l M free DNA. Dashed line: (A,C,E,G,I) complex of DNA with DDI-1A (50 ... of the various repeats and the effect of diastereomers DDI-1A and DDI-1B with 32 P-primer strands. A standard reaction (23 °C, 24 h) containing 5¢- 32 P-end-labeled primer and unlabeled template ... agents (DDIs and doxorubicin). The DNA bulge binding of both compounds was detected by CD and UV melting experiments. Possible slippage mechanisms are discussed. Results and Discussion Effect of DDI-1A and...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... positive strand, and the sequences AA AGA AAAG () 651 to ) 643), AAAGAAAAG () 725 to ) 717) and GAAGACATT() 849 to ) 857) are pre- sent in the negative strand. Given that the caspase-8 and caspase-10 ... similarity and 21% identity to other known death effector domains (DEDs), and 39.2% similarity and 26.7% identity with the pDED of HIP1. It has been shown that the inter- action of HIPPI with HIP1 ... 1B, panels I and II). A summary of the results is shown in Table 1. From the experimental studies described above with the various mutant motifs and their interactions in vitro with HIPPI, the...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: "Japanese Named Entity Recognition based on a Simple Rule Generator and Decision Tree Learning" pdf
... starting points and select the earliest ones. If two or more candidates start at the same point, their ending points are compared and the longest candidate is selected. Therefore, the candidates overlapping ... the selected candidate are removed from the candidate set. Thisprocedure is repeated until the candidate set becomes empty. The rank of a candidate starting at the - th word boundary and ending ... Results Now, we compare our method with the ME system. We used the standard IREX training data (CRL NE 1.4 MB and NERT 30 KB) and the formal run test data (GENERAL and AR- REST). When human annotators...
Ngày tải lên: 08/03/2014, 05:20
Báo cáo khoa học: "A Uniform Treatment of Pragmatic Inferences in Simple and Complex Utterances and Sequences of Utterances" pot
... in Common Lisp with Screamer. This algorithm computes uni- formly pragmatic inferences that are associated with simple and complex utterances and sequences of ut- terances, and allows cancellations ... sunderstandings and non-understandings. Speech Communication, 15:213-229. G. Hirst. 1991. Existence assumptions in knowledge representation. Artificial Intelligence, 49:199-242. L. Karttunen and ... Presupposition and entail- ment. In Oh C.K. and Dinneen D.A, editors, Syn- ta~ and Semantics, Presupposition, volume 11, pa- ges 155-182. Academic Press. H. Zeevat. 1992. Presupposition and accommoda-...
Ngày tải lên: 08/03/2014, 07:20
Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... (Roche) and incubated with shaking for 20 h at 30 °C. The supernatant was removed and a further 250 lL buffer F without trypsin was added for 4 h. The combined supernatants were dried to 50 lL and ... buffer C and fractionated over 20 mL with a0–0.5 M NaCl gradient in buffer C with 1-mL fractions. Peak fractions were pooled and dialysed into buffer A, concentrated to less than 200 lL, and chromatographed on ... AMP from NADH, ATP and FAD by purified NR/GS inhibitor in a 100-lL incubation at 30 °C for 10 min, using the amounts and concentrations of ATP and NADH used in standard GS and NR assays, respectively....
Ngày tải lên: 08/03/2014, 08:20
Internal audit in banks and the supervisor’s relationship with auditors: A survey pdf
... its estimate of risk; and - the systems established to ensure compliance with legal and regulatory requirements, codes of conduct and the implementation of policies and procedures; 3 ... policies and procedures; • an operational audit aims to assess the quality and appropriateness of other systems and procedures, to analyse the organisational structures with a critical mind, and ... management and financial information systems, including the electronic information system and electronic banking services; - the accuracy and reliability of the accounting records and financial...
Ngày tải lên: 11/03/2014, 02:20
Computer and Internet Use Among People with Disabilities pdf
Ngày tải lên: 15/03/2014, 22:20
Autobiographical Reminiscences with Family Letters and Notes on Music pdf
Ngày tải lên: 16/03/2014, 01:20
Health equity and financial protection streamline analysis with adept software pdf
Ngày tải lên: 22/03/2014, 16:21
Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf
Ngày tải lên: 23/03/2014, 13:20
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