signi cance of enzyme activity for substrate supply

... hoạt tính enzyme -D fructofuranosidase chế phẩm pectinex Ultra SP-L v h m lợng đờng saccharose dịch mía Để có định hớng sử dụng chế phẩm enzyme pectinex-Ultra SP-L, hoạt tính enzyme -D frutofuranosidase ... tăng lợng enzyme, ngợc lại h m lợng glucose tăng dần theo chiều tăng lợng enzyme Số liệu cho thấy khả thủy phân enzyme tăng dần tăng nồng độ enzyme Kết thu đợc bảng cho phép chọn tỷ lệ enzyme/ cơ ... tỏ hoạt tính transferase enzyme tăng nồng độ enzyme tăng Khi lợng enzyme tăng từ 0,005 - 0,02 h m lợng fructose đợc vận chuyển tạo FOS tăng từ 0,6% đến 6,9% Nhng lợng enzyme tăng từ 0,02 đến 0,04...

Ngày tải lên: 29/08/2013, 08:16

... affinity for substrates was unchanged, whereas catalytic activity was altered because the kcat value had decreased dramatically The structural study showed that the backbone conformation of the enzyme ... the bulky and rigid structure of histidine that can cause steric hindrances for the substrate, wherefore correct positioning and stabilization of the negative charge of E52 will be less than optimal ... changed, and the increase in the size of the side chain may create steric hindrance for the substrate, making the base moiety of the substrates bind in a nonoptimal conformation Another interesting feature...

Ngày tải lên: 19/02/2014, 02:20

... benefits of physical activity for health and well-being, as well as the current context of women’s health and participation in physical activity The general benefits of physical activity for health ... study of customary or habitual physical activity that found activity failed to significantly predict levels of morale or 38 BEYOND SHAPE AND SIZE: THE HEALTH BENEFITS OF PHYSICAL ACTIVITY FOR GIRLS ... equally so of women who are coping with many forms of diseases including breast cancer, heart disease and osteoporosis The activity can take many forms, from the joyous exertions of dragon boat...

Ngày tải lên: 05/03/2014, 13:20

... and effect of Ca2+ on the GTPase activity of recombinant TG2s GTPase activity was calculated as percentage of activity of the WT [99.1 ± 7.4 pmol GTP (minÆmg protein))1] in the absence of Ca2+ Data ... Transglutaminase activity was measured using mm CaCl2 and 0.4 lg of TG2 The reaction was started with 50 lL of enzyme mixture, and was performed at 37 °C for 30 Filter paper assay of transglutaminase activity ... Transglutaminase activity is shown as a percentage of the activity of wild-type TG2 One hundred per cent specific activity of wild-type TG2 was 8.4 DA405 (minÆmg))1 protein in the case of the microtiter...

Ngày tải lên: 16/03/2014, 03:20

... full-length T-AChE: The activity of full-length T-AChE is not signi cantly enhanced for any of its oligomers The activity displayed corresponds to the absolute activity measured for full-length T-AChE ... Neurite outgrowth (µm) A Fig Effects of T-AChE C-terminal peptides on a7-nAChR (A) Comparison of C-terminal amino acid sequences of R- and T-AChE isoforms Unique isoform sequences are underlined; arrows ... (filled rectangle) The activity of detergent-boosted T548-acetylcholinesterase is signi cantly higher than the activity of not-enhanced T548-acetylcholinesterase (Right) Enhancement of sucrose density...

Ngày tải lên: 23/03/2014, 07:20

... hydrolysis of substrate by HAP must function by an alternative mode of action [5] Resolution of a crystal structure for HAP would provide considerable insight into the unique functionality of this enzyme ... Standard dilutions of commercial BSA were used to generate a standard curve pH of maximal activity, enzyme kinetic assays and inhibition studies The pH of maximal activity for wild-type HAP and ... possible that its alteration to Lys is of functional signi cance Wild-type HAP, H34A and S37A exhibited a broad pH of maximal activity (5.0–8.5) (Fig 3) Activity in the basic pH range would also...

Ngày tải lên: 23/03/2014, 07:20

... redox state of the cysteinyl residues of apoFNR should be relevant for function and properties of the protein Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ‘aerobic’ ... conditions used here for FNR inactivation 4265 Disulfides of apoFNR ApoFNR is a product of the reaction of ([4Fe-4S]ÆFNR) with air The in vitro rate for the conversion of [4Fe-4S]ÆFNR to apoFNR (via [2Fe-2S]ÆFNR) ... on the redox state of the cysteinyl residues The rate of apoFNR formation is similar to that of [4Fe-4S] ⁄ [2Fe-2S] conversion, indicating that apoFNR is a signi cant product of FNR inactivation...

Ngày tải lên: 23/03/2014, 15:20

... used for activity assay or analysis by starch gel electrophoresis [2] After electrophoresis, gels were stained for ADH activity using 100 mM 2-buten-1-ol as a substrate Also, ADH activity of homogenates ... ADH1 shows substrate inhibition [31] and the contribution of ADH3 is still negligible because of its extremely low activity at pH 7.5 [7] One activity unit corresponds to the reduction of lmol NAD ... Detection of ADH1 and ADH4 in tissue homogenates of the gastrointestinal tract (A) Starch gel electrophoresis stained for activity using 100 mM 2-buten-1-ol as a substrate (B) Graphic representation of...

Ngày tải lên: 23/03/2014, 17:22

... year for which SSS is > 0.85 (number of trees needed) b Table II Summary of the regression coefficients expressing the effects of climatic factors on chronologies of Pinus pinea The signal of the ... of radial increment of P pinea was higher Climatic signi cance of IADFs in Pinus pinea 235 Figure Stabilized IADFs frequency in relation to calendar years The dot line represents the number of ... decreases the supply of carbohydrates for latewood formation and secondary thickening of cell walls This can explain the negative correlations between temperature in August(t) and latewood formation...

Ngày tải lên: 07/08/2014, 16:20

... structure of preferred substrates of TGase This information should be useful for predicting substrate reactivity and designing regulatory molecules that can interact with the active site of the enzyme ... effect on the reactivity of the peptide Assessment of the K5 sequence for reactivity and specificity in the peptide form To assess the reactivity and specificity of K5 in the peptide form, biotinylated ... function as substrates with similar levels of reactivity The reactivity and specificity of K5 for TGase was maintained even in peptide form Initially, we expected that the lysine residue of K5 would...

Ngày tải lên: 23/03/2014, 06:20

... demonstrate the validity of this last statement, we compared the performance of a simple integrator for the ZDP1-reduced PTS system against that of a state -of- theart integrator for the full PTS system ... the autokinase activity of growth factor receptors, the difference between substrate and enzyme is blurred By consequence, the vast separation in the concentrations of enzymes and substrates disappears ... case with enzymes acting on polynucleotides (such as DNA gyrase and ribosomes), where the concentrations of enzymes and binding sites are often of the same order of magnitude In all of these cases,...

Ngày tải lên: 18/02/2014, 06:20

... of Tma-RNase HI and substrate Importance of HBD for Mg2+-dependent activity Removal of the HBD severely reduces the Mg2+dependent activity of Tma-RNase HI by 750-fold without signi cantly affecting ... conformation of the active site is not signi cantly changed on removal of the HBD Because the HBD is important for substrate binding, the HBD may affect the interaction between the enzyme and substrate ... Role of HBD from T maritima RNase HI N Jongruja et al optical path length of cm and an A280 value for 0.1% (1 mgÆmL)1) solution of 1.79 for Tma-RNase HI, 1.75 for Tma-CD, 1.85 for Tma-ND, 1.58 for...

Ngày tải lên: 06/03/2014, 22:21

... to the substrate binding site of the enzyme J Biol Chem 245, 454465 32 Hiromi, K (1970) Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme- catalyzed ... over kcat/Km for amylose DP17 in the range of 100140, while the ratios were around 60 for Met53Trp/Tyr and for wild-type The noted expansion to fourfold variation of the ratio of the activity towards ... combination of the present mutations and mutations at other subsites can accentuate the suppression of activity for shorter substrates and further develop the enzyme specicity as done recently for dual...

Ngày tải lên: 31/03/2014, 08:20

... the Page 11 of 16 field of PA programmes for elderly, the AACVPR advises that information management involves supervision of the storage, communication, utilization and tracking of information ... effective method of promoting a PA programme for this target population Across all programmes, 76,92% offer different forms of access to facilitate the enrolment of seniors The Task Force on Community ... performance and the perception of quality of the service produced [90] One of the most commonly used techniques for listening to customers is satisfaction surveys [44] More than three quarters of...

Ngày tải lên: 14/02/2014, 06:20

... is a signi cantly higher number of proteins than were detected in the previous gel-based study [76] Therefore, our COFRADIC technique allows the functional interpretation of a larger part of a ... reached In contrast, using COFRADIC, we select subsets of peptides related to the biological question under investigation For instance, for the study of the oxidation of protein methionines during ... [38] The characterization of protease substrates by such a differential N-terminal COFRADIC approach is illustrated in Fig In an ongoing project, host cell substrates of the HIV-1 protease are...

Ngày tải lên: 18/02/2014, 16:20

... constant of most of the amide protons was in the range of 6–8 Hz, suggestive of rapidly interconverting conformers that coexist in solution The temperature coefficients of chemical shift of amides ... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... conformation of the peptide are important for biological activity Truncation of the cVY peptide to the hexapeptide cYT resulted in drastically reduced biological activity suggesting the lack of...

Ngày tải lên: 19/02/2014, 13:20

... (0.8 UÆmL)1) After incubation for 10 at 24 °C, the absorbance at 375 nm was read One unit of enzyme activity was defined as the amount of enzyme that converted lmol of 2-hydroxymuconic 6-semialdehyde ... molar extinction coefficient of 4.4 · 104 for 2-hydroxymuconic 6-semialdehyde was used [12] Specific activity was Enzyme purification All steps of the purification of the enzyme that used 2-amino-5-carboxymuconic ... Purification and properties of the purified enzyme The activity of the enzyme present in the crude extract of strain 10d that used 2-amino-5-carboxymuconic 6-semialdehyde as substrate was measured...

Ngày tải lên: 19/02/2014, 16:20

... 2.5 kb upstream of the SacI site) and XbaI (approximately 1.2 kb upstream of the SacI) sites (Fig 1) Fig Promoter activity of serial deletion constructs of the 5¢-flanking region of the mouse integrin ... luciferase activity was determined in triplicate, and data were normalized to b-galactosidase activity Fig Promoter activity of serial deletion constructs of the 5¢-flanking region of the mouse ... [57] The former enzyme is responsible for the synthesis of the b1–6 branch in N-acetyllactosamine units in cell surface N-glycans, and the latter enzyme is involved in the synthesis of cell surface...

Ngày tải lên: 21/02/2014, 03:20

... [22,26] Clearly, it is of interest to take advantage of the wealth of structural information available for Mb and apoMb for the comparative study of the molecular features of the homologous Ngb ... Professor Isabelle Mansuy of the University of Zurich for insightful discussions PP is ¨ a recipient of a Marie Curie Intra-European fellowship and SD is a post-doctoral fellow of the Fund for ... environment of mouse neuroglobin: evidence for the presence of two conformations of the heme pocket J Biol Chem 276, 36377–36382 Bashford D, Chothia C & Lesk AM (1987) Determinants of a protein...

Ngày tải lên: 07/03/2014, 02:20

... mapped sites for phosphorylation and ubiquitination, and studied the oligomerization state of the enzyme in vitro and in cells The kinetic parameters for the hydrolysis of ubiquitin substrates ... N- and C-terminal domains of USP7 affected the activity of the enzyme, with the C-terminus having a major impact Interestingly, this region appears to be required for enzyme oligomerization Finally, ... inhibitor of jB kinase, a regulatory role can be presumed for USP7 phorsphorylation The identification of K869 as the ubiquitination site of USP7 represents additional evidence for the interaction of...

Ngày tải lên: 07/03/2014, 05:20