signaling pathways in the normal and neoplastic breast
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... The increased activity of NOS and the increased formation of NO in brain in anoxia as observed by increase in NOx concentration in this study support the earlier findings of NO involvement in...
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Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
... looses the ability to buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca2+]c, probably coincides with the cleavage of the exchanger (but see [164]) Therefore, ... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... Na+ and K+ homeostasis in neurodegeneration, is currently unknown Nevertheless, the fact that these proteins are intensely expressed in the central nervous system [251,254,261] and their ever-increasing...
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Báo cáo khoa học: Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress doc
... is involved in multiple signaling pathways a wide range of substrates, and it has been suggested to be the integrative point of multiple pathways [3] The MAPK cascade consists of three interlinked ... orthologs in other plant species, including MPK3 and MPK6 in Arabidopsis (group A MAPKs), SIMK and SAMK in alfalfa, and LeMPK1 ⁄ (Lycopersicon esculentum SA-induced protein kinase) and LeMPK3 ... CMV, and the 10th true leaves were used for the determination of virus accumulation and photography Fourteen days after the inoculation, leaf curling, stunting and other abnormalities appeared in...
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Báo cáo khoa học: Yeast oxidative stress response Influences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot
... accessibility of Yap1 to the GSH1 promoter could be repressed by Cbf1, a DNA-binding protein that binds to elements in the vicinity of Yap1 binding site [52] Alternatively, the increased reducing power of ... treated during 40 with no agent as a control or with 1.2 mM H2O2 and 0.1 lgÆmL)1 antimycin A (anti A), singly or in association, as indicated in the figure The symbols –, + or ± in the last column ... cTPxI and Yap1 is becoming evident, although its molecular basis is not yet clear It was demonstrated that Yap1 is retained in the nucleus in the presence of H2O2 and thereby it interacts with the...
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Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx
... clear that the plant protein is not able to bind metal cations in the conformation found in the crystal structure Neither the first nor the other domains are able to coordinate cations in these regions, ... of the membrane binding loops with AnxA5 and Anx24(Ca32) reveals that canonical calcium binding in the loops of domain I might be possible in Anx(Gh1), in contrast to the bell pepper annexin The ... remarkable since the protein was never kept under reducing conditions Similar situations have been observed in other proteins, such as the fatty acid binding protein [32] and cyclophilins [33] The electron...
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Báo cáo khoa học: Photodynamic treatment and H2O2-induced oxidative stress result in different patterns of cellular protein oxidation ppt
... to determine the sites of the oxidative modifications within the tubulin molecule, and to elucidate the role of these modifications in the functional damage to tubulin Interestingly, for the two ... patterns of protein labeling were distinctly different (Fig 4A,B) Matching the blot with the protein map shows that PDT caused labeling of a-tubulin and b-tubulin (spots and 2) The minor spot probably ... are shown in (A) and (B) Silver staining is shown in (C) and (D) in blue superimposed with the spots of oxidized proteins shown in red Protein labels: 1, a-tubulin; 2, b-tubulin; 3, actin; 4, PDI;...
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báo cáo hóa học: " Brain inflammation and oxidative stress in a transgenic mouse model of Alzheimer-like brain amyloidosis" pptx
... inflammation and Aβ levels in addition to the deposition of Aβ in brain [13,14] Despite this evidence, and the considerable theoretical and therapeutic interest, the relationship between brain inflammation ... as inflammation Thus, we observed that this antioxidant further suppressed both amyloidosis and brain inflammation when combined with indomethacin In summary, our findings support the hypothesis ... operative in the AD brain and significantly contribute to the pathophysiology of the disease Although classical defined inflammation, including such features as edema and neutrophil invasion,...
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Báo cáo y học: "Nifedipine decreases sVCAM-1 concentrations and oxidative stress in systemic sclerosis but does not affect the concentrations of vascular endothelial growth factor or its soluble receptor" potx
... for sVEGFR-1, and less than ng/ml for sVCAM-1 Materials and methods The onset of the disease was defined as the time at which skin involvement occurred The laboratory tests included the Westergren ... production, including hypoxic conditions and stimulation by transforming growth factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between ... dinitrophenylhydrazine in M HCl, or with 0.5 ml of M HCl alone for the blank Samples were incubated for hour at room temperature in the dark, and then treated with 10% trichloroacetic acid and centrifuged The...
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Báo cáo y học: " Impaired glucose transporter-1 degradation and increased glucose transport and oxidative stress in response to high glucose in chondrocytes from osteoarthritic versus normal human cartilag" pps
... degradation in late OA In another study, exposure to either low or high glucose concentrations induced insulin-like growth factor-1 The aim of the present study was therefore to determine and compare the ... collected normal and OA cartilage and participated in the study design AM collaborated in the 2-DG uptake assays and the study design, and revised the manuscript CL participated in the study ... were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein assay kit (Sigma Chemical) The samples (25 μg protein) and molecular...
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Báo cáo khoa hoc:" Lack of association between mutations of gene-encoding mitochondrial D310 (displacement loop) mononucleotide repeat and oxidative stress in chronic dialysis patients in Taiwan" ppsx
... Competing interests The authors declare that they have no competing interests Authors' contributions J-BC participated in the design of the study and drafted the manuscript T-KL provided the intellectual ... oxidative damage, we intend to further investigate the possible link between oxidative stress and mtDNA mutations mtDNA deletions/insertions and base changes mainly involve purine transitions, which ... mutations in the D310 region; this finding may explain the high frequency of homoplasmic D310 somatic mutations in many types of tumors [13] The number of cytosines in the first stretch of the D310...
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Báo cáo y học: "Differential expression of copper-associated and oxidative stress related proteins in a new variant of copper toxicosis in Doberman pinscher" potx
... CASH and DH groups The third copper chaperone CCS, responsible for the transport of copper to SOD1, is inhibited 8-fold in the DH group, 2-fold in the CASH group, and remained unchanged in the ... dramatic changes were found in the mRNA levels of the cell-cycle inhibitor p27KIP which is inhibited 24-fold in the DH group, 12fold in the CASH group, and 3-fold in the N-CASH group Western blots ... valid and the average amount was included in the study (data not shown) Results were normalized according to the average amount of the endogenous references The normalized values were divided by the...
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Báo cáo y học: "Boswellic acids extract attenuates pulmonary fibrosis induced by bleomycin and oxidative stress from gamma irradiation in rats" doc
... [35], or inhibiting TGF-ß pathways, through a negative regulator of TGF-ß1 signaling [36] Therefore, therapeutic strategies to inhibit TGF-ß1 by interfering with the receptor signaling process ... determined according to Sinha [58] Histological examination The lungs were removed, inflated at 25 cm pressure with PBS then fixed, embedded in paraffin, sectioned and stained with hematoxylin and ... promising effects on these inflammatory parameters The anti-fibrotic action of BA may attenuate the events following the initial cell damage and the acute inflammatory phase of lung injury [60] The...
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Báo cáo y học: "Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats" potx
... participated in the surgical procedure, in in vitro measurements and in the design of the protocol, and drafted the manuscript MB carried out the Western blotting MdlB and LF carried out the surgical ... NaHS on inflammatory pathway signaling (a, d) Western blots revealing NF-kB expression in the aorta (a) and in the heart (d) (b, e) Western blots revealing P-IB expression in aorta (b) and in heart ... Amplitude/Wd, n = 10) in the aorta (a) and heart (c) of the two groups of rats (b, d) Western blots revealing iNOS expression in the in the whole lysate of aortas (n = 6) (b) and in hearts (n = 6)...
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Tài liệu Phytohormones and Abiotic Stress Tolerance in Plants ppt
... harpin-induced 1) was increased in tobacco leaves On the other hand, spermine induces mitochondrial dysfunction and activation of SA-induced protein kinase (SIPK) and wound-induced protein kinase ... cytokinins (zeatin and zeatin riboside) are decreased in the xylem in response to drought stress Surprisingly, in the same study, it was found that the level of the aromatic cytokinin 6-benzylaminopurine ... (Yamaguchi-Shinozaki and Shinozaki 2006) ZmABI4 interacts specifically with CE and functions in ABA signaling during germination and in sugar sensing in maize (Niu et al 2002) Among the group A...
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Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt
... scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose (50 mM) to determine if the ... was incorporated inside the ghost cells Ghosts resealed in the presence of acrystallin showed the extra two a-crystallin subunit bands at around 20 kDa confirming the efficient sealing of the protein ... in tubes in an ice bath to prevent resealing The ghost cells eluted in the void volume (70 mL) while the main haemoglobin band followed about 130 mL later (Fig 1) The lysed cells were white and...
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báo cáo khoa học: " Characterization of singlet oxygen-accumulating mutants isolated in a screen for altered oxidative stress response in Chlamydomonas reinhardtii" ppsx
... overexpression was determined, they were excluded from further analysis The remaining 20 mutants, being either HL-sensitive and/ or t-BOOH resistant, were then tested for the expression of the endogenous ... of the wild-type and mutant phenotypes was found in strains 35H11 and 18F6 (Figure 2), indicating that mutations in a single nuclear gene is responsible for the increased GPXH expression in each ... increased lutein and zeaxanthin levels in this mutant Despite the important role of zeaxanthin and antheraxanthin in photoprotection and prevention of 1O2 generation, only moderate changes of these xanthophylls...
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Báo cáo y học: " Diametrically opposed effects of hypoxia and oxidative stress on two viral transactivators" pdf
... shown in Figure 1A, emphasizing the domains of these two proteins that are required to bind their cognate recognition sites on DNA or RNA, and the domains that are redox-responsive EBNA1's DNA-binding ... luciferase reporter in multiple cell-lines including epithelial lines such as 293 and the Hela derivative TZM-bl Therefore, after confirming that Tat transactivated an HIV-LTR reporter in C33a cells ... Studying both transactivators in the same cell-line has permitted comparing them without the interpretational complications caused by using two different cell-lines Both reporter plasmids used the...
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Báo cáo khoa học: " Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis" ppt
... in sepsis [46] the writing of the manuscript All authors read and approved the manuscript Acknowledgements The authors thank Leandro Martins and Camila Valentin for their help in the recruitment ... AMPs, endotoxin and other bacterial products may be present in the milieu of circulating cells in septic patients and would support our diverging cytokine and ROS results This is further evidence ... contributions PSM and MKB participated in the design of the study, laboratory tests, and in the writing of the manuscript LSWM performed the laboratory tests FRM, MSA and SB recruited patients and discussed...
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Báo cáo y học: "Road traffic accidents and posttraumatic stress disorder in an orthopedic setting in south-eastern Nigeria: a controlled study" doc
... involved in the conception of the study OI participated in data collection and wrote the initial draft of the Manuscript OI, MOB and AOA analyzed the data All authors were involved in revision of the ... 1, p = 0.000) Gainfully employed prior to the RTAs was defined by the individual concerned having a source of income and independent; either being self employed, employed by the government or ... different in the distribution of their marital status with those in the control groups being more married than the victims of RTA (X2 = 20.70, df = 4, p = 0.000) They were also different in the distribution...
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spatial variability and terminal density implications in soil behavior
... renormalization for computing the equivalent hydraulic conductivity in the x direction Example Landau-Lifshitz-Matheron approximation and the geometric mean Landau and Lifshitz (1960) and Matheron ... is studied using various inclusion sizes and media There exists a fixed relation between the radius and the length of the inclusion The infinite hydraulic conductivity is modeled using a wire mesh ... 3.2 The influence of mesh size is investigated using the COMSOL code and the geometry and materials shown in Figure 2.8 Results in Figure 2.8 suggest a minimum number of ~6000 elements to attain...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25