short chain fatty acids in colon cancer cells

... apoptosis in SW480 human colon cancer cells To investigate the effects of IxoA on apoptosis, we 5720 used acridine orange ⁄ ethidium bromide staining and DAPI staining and found a marked increase in ... Conjugated linoleic acid inhibits peritoneal metastasis in human gastrointestinal cancer cells Int J Cancer 118, 571–576 Ogata S, Uehara H, Chen A & Itzkowitz SH (1992) Mucin gene expression in colonic ... chemopreventive agents, including resveratrol [26,27], brassinin [28,29], and deguelin [30,31] Resveratrol is present in grapes, red wine and peanuts, brassinin is from Chinese cabbage, and deguelin is from...

Ngày tải lên: 23/03/2014, 10:20

... differ in their C-terminal ligand binding domains, and each appears to bind and respond to a specific subset of agents including hypolipidemic drugs, long chain fatty acids, prostaglandins, and ... Modifying Factors in Asian Indian and Chinese Males Nutrition In press • Manav, HM Shen and CN Ong Involvement of c-Jun N-terminal kinase activation in Docosahexanoic acid-induced apoptosis in colon ... PPARγ ligands induced polyps only in the large intestine compared to the high-fat diet which 23 increased the polyp number in both the small and large intestines, suggesting that fatty acids may...

Ngày tải lên: 26/09/2015, 09:57

... Tanka-Salamon et al Fatty acids as plasmin inhibitors All three fatty acids examined caused a 10–20-fold increase in the Km of plasmin: oleate and arachidonate were efficient in the 10–65 lm concentration ... action of the fatty acids in the plasmin molecule, the amidolytic activity of two truncated plasmin variants was examined (Fig 8) Miniplasmin (des-kringle 1-4 plasmin) contains the kringle and the ... FEBS 1279 Fatty acids as plasmin inhibitors A Tanka-Salamon et al Fig Amidolytic activity of des-kringle plasmin derivatives in the presence of fatty acids The activity of 20 nM miniplasmin (A)...

Ngày tải lên: 18/02/2014, 17:20

... I, 10 nM insulin, E, lM epinephrine; P, 0.5 mM palmitate; P + I, palmitate + insulin; P + E, palmitate + epinephrine The bars indicate ± S.E.M Values are means of between five and nine independent ... long -chain fatty acids [50–52] However oleate was as effective as palmitate in causing activation of AMPK making an involvement of sphingolipid signalling unlikely Phosphorylation of Thr172 within ... possible (summarized in Fig 7) Activation of AMPK by fatty acids (Eur J Biochem.) Fig Summary of the interplay between the effects of long -chain fatty acid, insulin and epinephrine on AMPK activity...

Ngày tải lên: 07/03/2014, 15:20

... and protein levels in treated HT29 and HT29-R cells (Fig 5A,B) Then, HT29-R cells were incubated with increasing concentrations of the TOP1 inhibitor, camptothecin, in the presence and in the absence ... down-regulating vimentin in the absence and in the presence of MTX Targeting of vimentin was carried out by lipofecting aODN-VIM into parental HT29 cells causing a decrease of 60% in the expression ... annexin V-FITC (Bender MedSystems, Vienna, Austria) binding Twenty-four hours after MTX treatment, floating and adherent cells were collected, washed in NaCl ⁄ Pi and resuspended in annexin-binding...

Ngày tải lên: 07/03/2014, 16:20

... acid Involvement of peroxisomal b-oxidation in the chain shortening of C20 PMI-PUFAs It is known that peroxisomal b-oxidation of long -chain fatty acids in animals results in chain shortening with ... completion, but results in chain shortening by only a few b-oxidation cycles [18] Our data obtained from experiments using CHO cells clearly show that peroxisomal b-oxidation was involved in the formation ... PMI-PUFAs in the processes of oxidation [15] and chain elongation [16] in animal cells has also been investigated However, the metabolic fate of PMIPUFAs in animal cells is not fully understood In this...

Ngày tải lên: 23/03/2014, 09:20

... p53 protein increased in response to DCQ in all three cell lines except in p53+/+ cells exposed to hypoxia (Figure 6A); p21 protein also increased in all cell lines except in p53+/+ cells exposed ... protein in the human colon carcinoma cell line T-84, and in EMT6 mouse mammary carcinoma cells and Lewis Lung Carcinoma (LLC) cells [4,8] We also showed that DCQ inhibited cell proliferation and induced ... activates ATM in p53+/+ cells Next we investigated whether DCQ causes cell death in human colon cancer cells by inducing DNA damage and activating ATM, as similar effects have been observed in EMT6...

Ngày tải lên: 09/08/2014, 09:20

... despite reducing the number of viable cells in the WST-1 assay, ionizing radiation did not induce significant apoptotic nuclear fragmentation in LNCaP cells (Figure 2D) In line with these findings, ... inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate cancer cell lines Up to now our data revealed that ErPC3 is a potent inhibitor ... lost or inactivated in a variety of solid human tumors, including prostate cancer Increased levels of PIP3 trigger the recruitment of phosphatidylinositide-dependent serine/threonine kinase (PDK1)...

Ngày tải lên: 09/08/2014, 09:20

... radio-sensitizer in hypoxic cells DCQ radiosensitization in the FHs74Int normal intestinal cell line After establishing effects of DCQ and IR in cancer cells, we compared DCQ efficacy in normal cells For ... Human colon cancer cell lines (DLD1, HT-29, HCT-116, and SW-480) were grown in RPMI 1640 containing L-Glutamine and 25 mm HEPES All Cell cycle analysis using flow cytometry Following treatment, cells ... dose was calculated according to the formula: (colony no in treatment/colony no in control) × 100 Methods Cell culture FHs74Int normal human intestinal cells were cultured in Hybri-Care medium supplemented...

Ngày tải lên: 09/08/2014, 10:21

... PGE2-Induced Cell Migration in Human LoVo Colon Cancer Cells In the present study, we examined the effects of PGE2 on the migration ability in human LoVo colon cancer cells by culturing LoVo cells ... significantly inhibits PGE-induced activation of JNK1/2 within 30 min, and suppressed PGE2-induced expression of uPA and MMP-9 within 24 h in human LoVo colon cancer cells (Figure 4B) 17b-Estradiol Inhibits ... PGE2-induced progression in colon cancer remains unclear In the present study, we examined the effects of 17b-estradiol on PGE2-induced cellular motility in human LoVo colon cancer cells, and further...

Ngày tải lên: 10/08/2014, 10:20

... medium containing 10% FBS into each well, cultured cells continuously for one week Fixated cells with methanol for min, stained cells by hematoxylin for 30 min, counted the numbers of colony (more ... cell scored as Cells without brown staining scored as 0, with mild brown staining scored as 1, with moderate brown staining scored as 2, with intense brown staining scored as The final positive ... (Hematoxylin staining, × 100) biomarker for colon cancer, gastric carcinoma, lung cancer, and hepatocellular carcinoma [5-8] In this study, we detected high levels of MACC1 in ovarian cancer tissues...

Ngày tải lên: 10/08/2014, 10:21

... Angiogenesis in Gastric Cancer Cancer Microenviron 2009 in press 14 Waugh DJ, Wilson C: The interleukin-8 pathway in cancer Clin Cancer Res 2008, 14(21):6735-41 15 Nishimoto N: Interleukin-6 in rheumatoid ... factor induces protein kinase D-dependent production of proinflammatory cytokines in endothelial cells Am J Physiol Cell Physiol 2009, 296(4):C821-7 13 Kitadai Y: Cancer- Stromal Cell Interaction ... angiogenesis in gastric cancer [13], and acquisition of chemotherapeutic resistance in androgenindependent proliferation of prostate cancer cells [14] Finally, there is extensive experimental and clinical...

Ngày tải lên: 11/08/2014, 00:22

... of 575 nm (A) Fluorescence intensity analysis of intracellular adriamycin in gastric cancer cells (B) Adriamycin releasing index of gastric cancer cells The releasing index = (accumulation value ... retention in SGC7901/PrPC cells were further determined by flow cytometric analysis The adriamycin-releasing index in gastric cancer SGC7901 cells was calculated as follows: releasing index = (accumulation ... PI3K/Akt signaling pathway may lead to inhibition of the MDR induced by PrPC in gastric cancer cells The mechanism underlying PI3K/Akt-mediated PrPC-induced MDR in gastric cancer was further investigated...

Ngày tải lên: 07/03/2014, 03:20

... encoding a fatty acid x-hydroxylase, a putative epoxide hydrolase, several genes encoding enzymes involved in the synthesis of fatty acids in plastids, including the stearoyl acyl carrier protein ... monomer into the cutin In this context, it is interesting to note that inhibition studies allowed LeQueu et al [49] to demonstrate the involvement of a peroxygenase in the formation of cutin of ... cytochrome P450 in the epoxidation of a long -chain fatty acid was first demonstrated in spinach leaves more than three decades ago [20,23], a cytochrome P450 able to epoxidize fatty acids is still...

Ngày tải lên: 07/03/2014, 03:20

... followed increases in the unsaturation of plasma membrane fatty acids, in particular, linolenic acid Furthermore, revisiting these data, we find a direct correlation between the linolenic to linoleic ... erythrosin B sterols, have on membrane properties As in past reports, the joint use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing ... Specific activity in lmol PiÆmin)1Æmg)1 protein Line generated by linear regression (r ¼ 0.980) curve for a nonessential activation dependent on the linoleic/oleic ratio present in plasma membrane...

Ngày tải lên: 08/03/2014, 22:20

... x-hydroxylation x-hydroxylation in -chain hydroxylation in -chain hydroxylation x-hydroxylation x-hydroxylation in -chain hydroxylation Epoxidation Epoxidation in -chain hydroxylation in -chain hydroxylation x-hydroxylation ... to help attract insect pollinators, giving CYP77A6 an indirect role in reproduction It is possible that the introduction of in -chain hydroxyl allows cross-linking of cutin, leading to reticulation ... key step in exine formation during plant evolution The apparent absence of the CYP77 family in the moss genome [58] might indicate that cuticles based on cutin polyesters containing in -chain hydroxylated...

Ngày tải lên: 22/03/2014, 16:21

... can induce regression of human glioma brain cancers in vivo This might be achieved by the induction of telomere loss in cancer cells as shown in our in vitro experiments with glioma-derived brain ... DNA selectively in the cancer cells and by inducing cell proliferation in the normal cells Although these cancer cells showed different degrees of sensitivity to Ruta treatment in vitro, the bulk ... H2O2 only and in combination: A, human MGR1 brain cancer cells showing higher percentages of abnormal metaphases in H2O2- and Ruta 6-treated cells; B, normal human B-lymphoid cells showing more normal...

Ngày tải lên: 22/03/2014, 17:20

... declined in gastric cancer [35], we speculated that PTEN might participate in the VD3-induced apoptosis in gastric cancer cells To investigate this, undifferentiated HGC-27 adenocarcinoma cells ... melanoma cells, including those in myeloid leukemia, and breast, prostate, colon, skin and brain cancer [4– 8] Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was originally discovered ... application of vitamin D as a novel molecular target in gastric cancer therapies in association with the use of TSA ⁄ NaBu and 5-Aza Results Vitamin D induced apoptosis in gastric cancer cells through...

Ngày tải lên: 22/03/2014, 21:20

... progestin administration was thus associated with the beginning of growth inhibition by progestins GPR30 mRNA is regulated according to the dose of progestin, which also correlated with progestin-induced ... were expressed in MPA-treated cells, but not in the control cells at either 24 or 48 h in differential display analysis Progestins upregulate GPR30 mRNA in MCF-7 breast cancer cells Northern-blot ... and C-C chemokine receptors Chemokines [27,28] and angiotensin II [29] have both shown growth-inhibiting properties with different cancers Interestingly, an orphan receptor (GPR41 in the rat),...

Ngày tải lên: 24/03/2014, 00:21

... Y, Jiwajinda S & Ohigashi H (2004) Zerumbone, a sesquiterpene in subtropical ginger, suppresses skin tumor initiation and promotion stages in ICR mice Int J Cancer 110, 481–490 10 Sakinah SA, ... °C The instrument was programmed from an initial temperature of 40 °C (held for min) and increased at °CÆmin)1 until 80 °C, °CÆmin)1 until 180 °C, and 10 °CÆmin)1 until 240 °C (held for min) Helium ... (AB480831), Arabidopsis thaliana xanthoxin dehydrogenase (NP_175644), Citrus sinensis short- chain alcohol dehydrogenase (ADH82118), Solanum tuberosum short- chain dehydrogenase ⁄ reductase (AAT75153),...

Ngày tải lên: 28/03/2014, 22:20