shedding and cleavage of the d6 n terminus
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
Ngày tải lên :
16/03/2014, 23:20
... detection by western blot analysis was limited to our most sensitive assay for PS1 N- terminal fragment (NTF) Fractions were analysed for the presence of C101-3FLAG and PS1 NTF and the signals quantified ... mainly in fractions 10 and 11 (B) Fractions from the 0.25% CHAPSO column were analysed for the presence of mature (mat) and immature (imm) nicastrin (Nct), PS1 NTF, PS1 CTF, and PS2 by western ... Wolfe MS (2004) Detergentdependent dissociation of active gamma–secretase reveals an interaction between Pen-2 and PS1-NTF and offers a model for subunit organization within the complex Biochemistry...
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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt
Ngày tải lên :
23/03/2014, 06:20
... ClpC1D2 contains the N- terminal repeat I and the intervening sequence between repeats I and II deleted, and the N- terminal repeat II forms its N- terminus (Fig 2B) The deletion mutants were also ... along with the intervening amino acids linking it to N- terminal conserved repeat II did not affect the conformation of ClpC1 and the resultant protein, ClpC1D2, had full enzymatic and chaperone ... is known about their specific functions ClpC consists of two AAA+ domains, the first of which contains an additional N- domain, homologous to the N- domains of ClpA or ClpB, and a linker domain homologous...
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Báo cáo khoa học: The equinatoxin N-terminus is transferred across planar lipid membranes and helps to stabilize the transmembrane pore pot
Ngày tải lên :
23/03/2014, 10:20
... and linker containing arginine to become closer to the trans entrance of the pore, to enter the pore lumen and to clog it The rate of closures was voltage-dependent and increased with the magnitude ... K Kristan et al Topology of equinatoxin II N- terminus A Fig Structure of EqtII and alignments of actinoporin N- terminal sequences, EqtII and mutants used in the study (A) Three-dimensional structure ... than the rest of the membrane-bound molecule; and (c) the first five amino acids help to stabilize the final pore The position of the first and third amino acids in the final pore Fig The effect of the...
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Báo cáo khoa học: Stabilities and activities of the N- and C-domains of FKBP22 from a psychrotrophic bacterium overproduced in Escherichia coli pptx
Ngày tải lên :
23/03/2014, 13:20
... composed of N- and C-domains, which are spanned by a 40 amino acid long a3 helix The N- domain consists of a1 and a2 helices and an N- terminal region of a3 helix The C-domain consists of six b-strands ... from the substrate when the peptide bond N- terminal of the proline residue is in the cis conformation The concentration of p-nitroaniline was determined from the absorption at 390 nm with the ... kn) ⁄ E, where E represents the concentration of the enzyme, and kp and kn 640 Y Suzuki et al represent the first-order rate constants for the release of p-nitroaniline from the substrate in the...
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Báo cáo Y học: Thermodynamics and kinetics of the cleavage of DNA catalyzed by bleomycin A5 A microcalorimetric study pdf
Ngày tải lên :
24/03/2014, 04:21
... between DNA and L The intrinsic binding constant, KB, is defined by the equation [24,28,29]: KB ¼ y ð1 À yÞð½DNA0 À ny½L0 Þ ð14Þ Here, [DNA]0 and [L]0 are the initial concentrations of DNA and ... s, the lifetime of self-inactivation, was calculated using the rate constant k1 A thermodynamic model for the binding of small molecules to DNA Understanding the thermodynamics of the binding of ... that DNA was not added to the reference cell For the experiments on DNA binding, compartment I of the reaction cell contained mL of a DNA solution and compartment II of the reaction cell contained...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
Ngày tải lên :
30/03/2014, 16:20
... particular denaturant concentration; the standard free energy change (DG°) is the free energy change at zero denaturant concentration; [D] is the denaturant concentration and m is the corresponding slope ... with increasing concentration of urea in the absence and presence of TMAO The Dm values for the urea-induced unfolding of FtsZ were found to be 1.8 and 3.6 m in the absence and presence of TMAO, ... 0.19 both in the absence and presence of 0.8 m TMAO (data not shown) Thus, the reduction in the fluorescence intensity of the FtsZ–ANS complex with increasing concentration of TMAO was not due a...
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Báo cáo toán học: "A multipartite version of the Tur´n problem a density conditions and eigenvalues" doc
Ngày tải lên :
08/08/2014, 12:23
... combinations with at most D1 non-zero coefficients, so if we change the weights in X1 to the convex combination coefficients of an intersection point, then the new construction has at most D1 non-zero ... an optimal construction for the path Pr+1 , satisfying the conditions of Lemma 2.1, then gluing the leaves together, we get a construction for Cr , inducing no Cr , and having minimal edge density ... instead of a bound on the number of edges in the graph In this context, the solution must depend on the cardinality of the partition classes, though the asymptotic behavior would be interesting,...
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Báo cáo y học: " High cell density and latent membrane protein 1 expression induce cleavage of the mixed lineage leukemia gene at 11q23 in nasopharyngeal carcinoma cell line" ppt
Ngày tải lên :
10/08/2014, 05:21
... transfection process On the other hand, cells transfected with LMP1 expression plasmids (Fig 4C, lanes and 4) showed very distinct and intense bands of sizes smaller than kb DNA sequencing of these ... data and writing of manuscript PHCY have been involved in the detailed experimental design, acquisition of data, interpretation of data and analysis All authors read and approved the final manuscript ... expression plasmids were used, namely pcDNA and pTracer Since the green fluorescent protein (GFP) gene and the LMP1 gene are on the same plasmid vector (pTracer), the expression of GFP is indicative of...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
Ngày tải lên :
24/08/2014, 09:33
... friendship and mentorship iii ABSTRACT Derek S Woods The Influence of the Ku80 Carboxy -Terminus on Activation of the DNA-Dependent Protein Kinase and DNA Repair is Dependent on the Structure of ... DNA binding11 The N3 and N4 groups of the pyrimidine moiety provide sequence specificity of DNA cleavage by binding to the N3 - and N2 -amino groups of the guanine 5’ to the pyrimidine (py) cleavage ... also not restrained to one stage of the cell cycle and instead can occur at all stages17 Initiation of NHEJ requires the formation and activation of the DNA-dependent protein kinase (DNA-PK) Once...
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Molecular mechanisms underlying the regulation of the positioning and formation of the cleavage furrow in cytokinesis in mammalian cells
Ngày tải lên :
14/09/2015, 14:09
... attempted to understand the functions of the different populations of microtubules in mitotic spindle in positioning of the cleavage furrow 1.2.1.1 Roles of microtubules in positioning of the cleavage ... Actin depolymerizing factor (ADF)/cofilin is also likely involved in not only formation and maintenance of the contractile ring (Nakano and Mabuchi, 2006), but also the regulation of actin disassembly ... by binding to myosin II-ADP-Pi Blebbistatin and inhibit the release of the ADP Inhibit actin polymerization by binding to the barb end of the FCytochalasin D actin, and blocking the G-actin to...
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Báo cáo y học: "Mirror-Image Arachnoid Cysts in a Pair of Monozygotic Twins: A Case Report and Review of the Literature"
Ngày tải lên :
25/10/2012, 11:00
... diagnosed an AC in the other twin (twin B), when the concerned parents took him to our department Brain CT and MRI of twin B who did not have any com- 403 plaints revealed a mirror-imaging of the ... with the pathogenesis of temporal fossa AC Helland et al 20 found the Na+–K+–2Cl− cotransporter NKCC1 gene was escalated in AC and NKCC1 was present in the AC wall These finding indicated NKCC1 ... dystrophy in one of monozygotic twin girls J Med Genet 1986; 23: 494-500 Sommer IE, Ramsey NF, Mandl RC, et al Language lateralization in monozygotic twin pairs concordant and discordant for handedness...
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Báo cáo y học: "Aplasia and Agenesis of the Frontal Sinus in Turkish Individuals: A Retrospective Study Using Dental Volumetric Tomograph"
Ngày tải lên :
25/10/2012, 11:04
... relationships also depend upon the extent of pneumatization [11] The extent of pneumatization results in the individual size and shape of the frontal sinus An absence of pneumatization in the frontal ... frequency in our population and that in other populations may be due to the number of patients examined, the patient sample, and the difference in the examining techniques and equipment In addition, ... constitutional (age, gender, hormones, and craniofacial configuration) and environmental (climatic conditions and local inflammations) factors control the frontal sinus configuration within each...
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Báo cáo y học: " Post-traumatic glioma: Report of one case and review of the literature"
Ngày tải lên :
25/10/2012, 11:48
... suffering the head injury The below must be severe enough to cause brain contusion and a secondary reparative process The location of the impact and the tumor should correspond exactly one to the other ... severity and location of the traumatic brain injury and the subsequent formation of a neoplasm at the same site, can provide convincing evidence for the traumatic causation of a brain tumor Our case ... that CT scan documented the absence of tumor at the time of the brain injury We suggest that these conditions for the acceptance of a causal relationship between cranial trauma and brain tumor study...
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. Scope and Limitations of the Study
Ngày tải lên :
13/04/2013, 10:31
... teaching methods and their evaluation Vietnam can take advantage of international achievements in education and science and technology when designing and implementing the education and training renovation ... education and training in the years to come its relevance to the needs of the country’s industrialization and modernization International and regional standards should be taken into account in defining ... Government in the financing of education and training are the making of contributions, in which they take account of their revenue/profits and the number and level of qualified staff they employ Parents...
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Scope and Limitations of the Study
Ngày tải lên :
13/04/2013, 10:32
... as the science and art of military command as applied to the prevail planning and conduct of large-scale combat operations” The planning theme remains an important component of most management ... the kind of economic and human organization it is or intends to be, and the nature of the economic and non-economic contribution it intends to make to its shareholders, employees, customers and ... definitions of strategy Strategy is the determination of the basic long-term goals and objectives of an enterprises, and the adoption of courses of action and the allocation of resources necessary...
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Methodology and framework of the study
Ngày tải lên :
13/04/2013, 10:32
... simplified, the level of knowledge of the management organizations and officers in charge of investment are now improved The consolidation of the former SCCI and SPC into one Ministry, which is now the ... the other hand, this is also an advantageous condition for increasing income and improving living standard resulting in the booming development of civil construction and programs of building infrastructure ... also mentions about the trend of investment of the plastic industries in Asian nations Next, the chapter moves on to apply the Porter model for examining the pipe industry environment, the main business...
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