sf 1 and wilms apos tumor suppressor 1 wt 1

Báo cáo sinh học: "At the crossroads: AMP-activated kinase and the LKB1 tumor suppressor link cell proliferation to metabolic regulation" pdf

Ngày tải lên : 06/08/2014, 18:20

... which Snf1p is activated [17 ] Pak1p, Tos3p and Elm1p can all phosphorylate Snf1p at Thr 210 [17 -19 ] (indeed, Elm1p was selected in a proteomic screen for Snf1p Thr 210 kinases [19 ]); but neither single ... whether LKB1 - in complex with STRAD and MO25 isoform(s) - mediates the actions of leptin and adiponectin The link between LKB1 and PJS, and the identification of LKB1 as a tumor suppressor and now ... mammalian p 21- activated kinase -1, also abbreviated Pak1), Tos3p and Elm1p, were recently identified as Snf1p kinases [17 -19 ] Thus, massspectrometric analysis of proteins associated with the Snf1p complex...

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Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic proﬁle and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt

Ngày tải lên : 16/03/2014, 10:20

... mammalian lectins 10 5 10 6 10 7 10 8 10 9 11 0 11 1 11 2 11 3 11 4 11 5 and wild-type and lectin-gene-transfected tumor cells as targets Bioconjugate Chem 15 , 87–98 ´ Manning JC, Seyrek K, Kaltner H, Andre S, Sinowatz ... function of p16INK4a ´ S Andre et al 10 11 12 13 14 15 16 17 18 glycan interaction with the emerging versatile effector functionality of mammalian lectins Trends Glycosci Glycotechnol 18 , 1 37 Alhadeff ... function of p16INK4a 11 6 11 7 11 8 11 9 12 0 ´ S Andre et al CRC Handbook of Endoglycosidases and Glycamidases (Muramatsu T & Takahashi N, eds), pp 19 9–332 CRC Press, Boca Raton ´ Andre S, Kojima S,...

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Báo cáo khoa học: Tumor suppressor p16INK4a: Downregulation of galectin-3, an endogenous competitor of the pro-anoikis effector galectin-1, in a pancreatic carcinoma model pptx

Ngày tải lên : 29/03/2014, 21:20

... northern blotting and the de novo transcription rate by run-off assays B p16INK4a negatively affects Gal-3 mRNA availability 10 0 10 0 0 10 0 C 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 Fig p16INK4a restitution ... 30 20 10 0 ) 1 l tro on C L 00 -1 al -1 al G + G · µg l-3 (2 l-3 -1 + G al G L -1 al G L -3 50 -3 16 24 B (1 al al + µ (1 · µg Time (h) L m m g· (5 ) 1 ) 1 m · µg a a G L m (1 ) 1 ) 1 m · ... G -1 + G al G 80 C 40 Counts 12 0 B 10 0 10 1 10 2 Gal-3 binding (log fluorescence) 80 40 Counts 12 0 C 10 0 10 1 10 2 Gal -1 binding (log fluorescence) Fig Gal-3 inhibits Gal -1- stimulated anoikis and...

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Báo cáo khoa học: The tumor suppressor HIC1 (hypermethylated in cancer 1) is O-GlcNAc glycosylated doc

Ngày tải lên : 30/03/2014, 15:20

... domain and the C-terminal end To conﬁrm these results, the full-length HIC1 protein and two C-truncated HIC1 mutants (1 714 , 1 616 and 1 400; Fig 4A) were produced in reticulocyte lysates and then ... cancer 1 gene (HIC1) is a candidate tumor suppressor gene located on chromosome 17 p13.3, a region frequently hypermethylated or deleted in many types of solid tumors [ 21 23] In addition, HIC1 expression ... the 399– 714 construct and two deletion mutants (construct 399– 713 and construct 399– 711 ) were equally detected by the RL-2 antibodies (Fig 6B, lanes 1 3) suggesting that residues 712 – 714 were...

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tumor suppressor genes, volume 1

Ngày tải lên : 11/04/2014, 10:38

... including melanoma (11 1), ovarian cancer (11 2), prostate cancer (11 3), skin cancer (11 4), medulloblastoma (11 5), liver cancer (11 6 ,11 7), and endometrial cancer (11 8) Finally, AXIN1 mutations have ... Inst 90, 11 38 11 45 19 Lakhani, S R (19 99) The pathology of hereditary breast cancer Dis Markers 15 , 11 3 11 4 20 Foulkes, W D., Wong, N., Brunet, J S., and Narod, S A (19 98) BRCA mutations and survival ... Ludlow, J W and Skuse, G R (19 95) Viral oncoprotein binding to pRB, p107, p130, and p300 Virus Res 35, 11 3 12 1 21 Goodrich, D W., Wang, N P., Qian, Y W., Lee, E Y., and Lee, W H (19 91) The retinoblastoma...

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Báo cáo y học: " Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein" ppsx

Ngày tải lên : 13/08/2014, 09:20

... transformation of Tax1 in CTLL-2 (Dlg1-) Tax1 Tax1 C PBM D) Low transformation of Tax1 C in CTLL-2 (Dlg1-) Tax1 Tax1 X Tax1 PBM C X PBM PBM C Dlg1 Tax1 Tax1 Tax1 PBM Dlg1 Tax1 PBM C X PBM Dlg1 ... plasmids against mouse dlg1 sequences (nt1092 -11 11 and nt23 91- 2 410 ), human dlg1 (hDlg1) sequences (nt 213 5- 215 3 and nt2563-25 81) , chloramphenicol acetyltransferase, and renilla luciferase genes, ... http://www.retrovirology.com/content/3 /1/ 71 B) A) 20 LUC Dlg1 Tubulin Dlg1 -1 Cell number (x105) Dlg1 -1 Dlg1-3 CAT 15 Dlg1-3 CAT 10 LUC 0 Days Figure Dlg1 knockdown in CTLL-2 does not affect the cell growth phenotypes Dlg1 knockdown...

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Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor

Ngày tải lên : 25/10/2013, 21:20

... families of CKIs, the INK4 family (p16INK4A , p15INK4B , p18INK4C and p19INK4D ) and the CIP/KIP family (p21CIP1 , p27KIP1 and p57KIP2 ) INK4A and INK4B at Chr9p 21 are frequently mutated in human ... fetal liver, muscle and lens p107–/– p130–/– p107–/–; p130–/– Rb–/–; p107–/– Rb–/–; E2f1–/– Rb–/–; E2f3–/– Rb–/–; Id2–/– Rb–/–; p53–/– Rb–/–; p19–/– Myeloid hyperplasia and growth deﬁciency on Balb/c ... suppresses growth and promotes lineage-speciﬁc differentiation; yet p107 and p130 appear to act together to suppress growth It may be important to reassess the status of p107 and p130 mutations in tumors...

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Báo cáo khoa học: Recognition of DNA modiﬁed by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx

Ngày tải lên : 16/03/2014, 14:20

... and 10 lgÆmL )1 (1. 26 · 10 )7 and · 10 )9 M), respectively, and concentration of p53 was 3.9 · 10 )8 M rb values: (lane 1) ; 0. 012 5 (lanes 2,5,8); 0.025 (lanes 3,6,9); 0.05 (lanes 4,7 ,10 ) The oligonucleotide ... of DNA modiﬁed by antitumor cisplatin by ‘latent’ and ‘active’ protein p53 Biochem Pharmacol 65, 13 05 13 16 18 Bancroft DP, Lepre CA & Lippard SJ (19 90) Part -19 5 NMR kinetic and mechanistic studies ... bottom bands) The fragment was nonplatinated (control) (lane 1) or modiﬁed by trans-PtHMP at rb ¼ 7.5 · 10 )4, · 10 )4, 2.5 · 10 )4 or · 10 )4 (lanes 3–6, respectively); and by cisplatin at rb ¼ · 10 )3...

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Báo cáo khoa học: Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 doc

Ngày tải lên : 22/03/2014, 21:20

... 0.3 4.7 ± 1. 7 5 .1 ± 1. 3 6.5 ± 1. 0 1. 8 ± 0.4 3.0 ± 1. 6 19 000 ± 2000 2700 ± 600 > 400 000 A )12 .4 ± 0.3 )11 .2 ± 0.2 )11 .1 ± 0.3 )11 .0 ± 0.3 )11 .7 ± 0 .1 )11 .4 ± 0.3 )6.3 ± 0 .1 )7.5 ± 0 .1 – B DG was ... (kcalÆmol )1) KD (nM) Full-length protein HPV16 E7 2.4 ± 0.2 HPV18 E7 7.8 ± 0.5 HPV 11 E7 10 8 ± N-terminal domain HPV16 E7 8.6 ± 1. 3 HPV18 E7 12 .2 ± 0.8 HPV 11 E7 366 ± 25 DDGb (kcalÆmol )1) )11 .6 ± ... Materials and methods Fragment KD (nM) DGa (kcalÆmol )1) E7b E7( 21- 29) (LXCXE)b E7 (16 - 31) (LXCXE)b E7 (16 -40) (CR2)b E7 (16 -40)PP (CR2PP)b E7 (1- 40) (E7N)b E7 (1- 20) (CR1) E7(40-98) (E7C) BPV (1- 43) (BPV-N)...

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Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx

Ngày tải lên : 30/03/2014, 02:20

... –0.04 15 30 cm 1 1552 cm 1 1622 cm 1 16 51 cm 1 1.0 0.08 15 52 –0.06 0.8 0.6 0.4 0.2 0.0 16 51 –0.08 17 00 16 50 16 00 15 50 Wavenumber (cm 1) 20 30 D 50 60 70 40 Temperature (°C) 16 22 0 .12 0 .10 0.08 ... (kJÆmol )1) mol )1 M )1) (kJÆmol )1) (M) mol )1 M )1) (kJÆmol )1) (M) mol )1 M )1) (kJÆmol )1) mol )1) mol )1) Apo 4.7 +Zn2+ 2.5 +Ca2+ 3.9 89.9 35 .1 40.3 13 .0 3.5 2.9 – )14 .2 )5.2 4.5 4.0 4.8 78.9 50 .1 62.4 11 .2 ... 15 30 0.00 –0.02 –0.04 –0.06 –0.08 –0 .10 –0 .12 –0 .14 15 52 80 90 80 90 16 08 cm 1 1622 cm 1 1635 cm 1 1693 cm 1 1.0 % Variation second derivative ΔAbs (a.u.) C 0 .14 15 00 0.8 0.6 0.4 0.2 0.0 16 51...

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Báo cáo hóa học: " Multiplexed methylation profiles of tumor suppressor genes and clinical outcome in lung cancer" potx

Ngày tải lên : 18/06/2014, 16:20

... (18 .5) ( 31. 8) (9.4) (19 .5) (1. 7) (18 .5) ( 31. 8) (9.4) SFRP4-2 16 (29.6) 11 (50.0) (15 .6) 12 (26.0) (50.0) (29.6) 11 (50.0) (15 .6) DLC1 22 (40.7) 12 (54.5) 10 ( 31. 2) 18 (39 .1) (50.0) 13 (48 .1) 12 (54.5) ... (0) (18 .5) (18 .2) (12 .5) CACNA1A 18 (33.3) 11 (50.0) ( 21. 9) 13 (28.3) (66.7) (29.6) 11 (50.0) ( 21. 9) TIMP3 10 (18 .5) ( 31. 8) (9.4) (19 .6) (1. 7) (18 .5) ( 31. 8) (9.4) TIMP3-2 11 (20.4) ( 31. 8) (12 .5) ... (55.5) 14 (63.6) 15 (46.9) CACNA1G 21 (38.9) 12 (54.5) (28 .1) 19 ( 41. 3) (1. 7) 12 (44.4) 12 (54.5) (28 .1) TGIF 10 (18 .5) (22.7) (15 .6) (19 .5) (1. 7) (22.2) (22.7) (15 .6) BCL2 (14 .8) (18 .2) (12 .5) (17 .4)...

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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx

Ngày tải lên : 18/06/2014, 19:20

... SK-NEP -1 0.00 1. 82 HT 10 80 19 .37 5.92 SA-4 12 .53 1. 05 ** SW 19 61 64.55 3.04 HS 919 3. 01 5. 31 KATO-III 7.65 2 .16 SW 80 -5.00 3.93 -56.35 1. 45 ** SW 732 ** DLD -1 69 .19 65.59 1. 29 3 .19 HS 913 T Lung ... 0.83 11 .53 Thyroid (1/ 2) Brain (2/9) Breast (0/9) ** NU-04 69. 41 4.02 SKBR3 -0.09 13 .53 ** U 118 MG SW 598 51. 96 14 .29 1. 48 4 .14 MDA-MB-468 GI -10 1 -3.46 -6. 41 0.25 0.92 A172 2.26 4.97 SV-BR -1 -8.00 ... 41. 77 19 .15 * FaDu 30.98 4.45 HEY 22.20 3.87 RPMI 2650 SW 2224 17 .46 -13 .48 5. 01 11. 21 Caov-3 -14 6.53 2.69 MCF-7 16 .95 0.39 ** SW 579 68.97 3. 41 734B 16 .72 2.32 SW 19 49 43.90 13 .68 T47D 8.47 1. 23...

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Báo cáo y học: "p53 tumor suppressor gene mutations in fibroblast-like synoviocytes from erosion synovium and non-erosion synovium in rheumatoid arthritis" ppsx

Ngày tải lên : 09/08/2014, 06:22

... (Ala>Val) 1/ 7 RA7 None 0 /11 Codon 16 7 CAG>CAA (Gln>Gln) 1/ 10 Codon 312 ATC>ATT (Ile>Ile) 1/ 10 RA8 1/ 12 Codon 17 6 TGC>TAC (Cys>Tyr) 1/ 12 Codon 309 CCC>CCT (Pro>Pro) 1/ 12 Codon 239 AAC>AGC (Asn>Ser) 1/ 12 ... (Ala>Ala) 1/ 12 Codon 213 CGA>CAA (Arg>Gln) 1/ 11 Codon 213 CGA>CGG (Arg>Arg) 1/ 11 Codon 269 AGC>AAC (Ser>Asn) RA13 2/9 Deletion codon 304 – codon 337 RA12 Codon 307 GCA>GCT (Ala>Ala) 1/ 11 None 0 /11 Codon ... RA10 Synovial tissue Frequency 1/ 10 Codon 17 8 CAC>AC (deletion) 1/ 10 Codon 360 GGG>AGG (Gly>Arg) 1/ 10 Long deletion codon 14 3 – codon 220 1/ 10 Codon 213 CGA>CGG (Arg>Arg) 5 /10 a Frequency 1/ 10...

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Báo cáo y học: "Systematic detection of putative tumor suppressor genes through the combined use of exome and transcriptome sequencin" ppt

Ngày tải lên : 09/08/2014, 22:23

... H, Riggins GJ, Bigner DD, Zhao et al Genome Biology 2 010 , 11 :R 114 http://genomebiology.com/2 010 /11 /11 /R 114 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Karchin R, Papadopoulos N, Parmigiani G, et ... Genome Biology 2 010 , 11 :R 114 http://genomebiology.com/2 010 /11 /11 /R 114 Page of 14 Figure Validation of allele-specific expression events in HCC1954 The top trace is from cDNA, and the bottom trace ... Genome Biology 2 010 , 11 :R 114 http://genomebiology.com/2 010 /11 /11 /R 114 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 Identification of regions correlating MGMT promoter methylation and gene expression...

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báo cáo khoa học: "Retraction: The candidate tumor suppressor gene ECRG4 inhibits cancer cells migration and invasion in esophageal carcinoma" docx

Ngày tải lên : 10/08/2014, 10:21

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haracterization of tumor suppressor genes hDAB2IP and DLEC1 in hepatocellular carcinoma

Ngày tải lên : 11/09/2015, 16:07

... of p 21 by DLEC1 11 6 5 .1 Introduction 11 6 5.2 Results 11 8 5.2 .1 G1 Phase Arrest in Cell Cycle Induced by DLEC1 11 8 5.2.2 Growth Inhibition by DLEC1 is Independent of p53, p 21 and DNMT3B 11 9 5.2.3 ... Introduction 1. 1 Overview of Hepatocellular Carcinoma 1. 1 .1 Incidence and Mortality 1. 1.2 Demographic Factors 1. 1.3 Etiology of HCC 1. 1.4 Host Factors 1. 1.5 Diagnosis and Therapeutic Options 1. 2 13 Genetics ... cell size and induces G1 arrest in cell cycle 11 1 Figure 5 -1 The growth inhibition by DLEC1 is independent of p53, p 21 and DNMT3B 11 9 Figure 5-2 DLEC1 is a transcriptional modulator 12 1 Figure...

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Regulation and functional analysis of the tumor suppressor gene product, p53

Ngày tải lên : 15/09/2015, 17:10

... CAHPTER 1: INTRODUCTION 1. 1 p53, the tumour suppressor 1. 1 .1 p53, the “Guardian of the Genome” 1. 1.2 p53 and DNA damage response pathway 1. 1.3 DNA repair and p53 1. 1.4 Cell cycle arrest by p53 1. 1.5 ... polymorphism 13 1. 4.2 p53 polymorphism in human malignant diseases 13 III 1. 4.2 .1 Breast cancer 13 1. 4.2.2 Skin cancer 14 1. 4.2.3 Cervical cancer 14 1. 4.2.4 Gastric cancer 14 1. 4.2.5 Other cancers 15 1. 5 ... repair 1. 1.5 .1 Gadd45 activation by p53 1. 1.5.2 p53R2 activation by p53 1. 2 Functions of different domains of p53 1. 3 Drug and chemotherapy resistance by mutant p53 11 1. 4 p53 polymorphism 12 1. 4.1...

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Regulation and function of the novel candidate tumor suppressor gene dlec1 in the HCT116 colorectal cancer cell line

Ngày tải lên : 16/10/2015, 15:37

... pcDNA3 .1- 4 16 .38 47.88 19 .94 16 .69 DLEC1-3 7.07 57.60 25.25 14 .95 DLEC1-4 10 .14 55.67 18 .69 18 .12 DLEC1-5 8.64 53.40 26.05 16 . 61 DLEC1-6 14 .85 66.38 12 .00 8. 01 DLEC1-7 6.86 57. 31 18.46 17 .85 DLEC1 -11 ... cell line pcDNA3 .1- 3 DLEC1-4 DLEC1-7 pcDNA3 .1- 4 DLEC1-5 DLEC1 -11 DLEC1-3 DLEC1-6 DLEC1 -15 Figure 5 .1. 4 DLEC1 over-expression induced cell cycle arrest or apoptosis in HCT 116 cells Stable clones ... vivo and/ or in vitro (Goyette et al., 19 92) 2.3 .1. 1 DLEC1 as a candidate tumor suppressor gene in colorectal carcinoma DLEC1 (deleted in lung and esophageal cancer 1) was first identified in 19 99...

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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"

Ngày tải lên : 25/10/2012, 11:15

... The sequence identity and homology between RIZ1 and EphR were 34.2% and 68.4% in the residues 63-99 region, 37.0% and 81. 5% in the residues 7 71- 802 region, and 22.9% and 53 .1% in the residues 924-973 ... models and concepts Annu Rev Med 2007; 58: 267-84 The authors declare that no conflict of interest exists References 10 11 12 13 14 15 16 Jiang G, Liu L, Buyse IM, Simon D, Huang S Decreased RIZ1 ... 11 -35% breast cancer cells sustained tumor growth through cell division [33], implicating that RIZ1 was likely overexpressed and acted as a tumor suppressor to inhibit breast cancer cell growth...

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