sets gene expression and the fat mass phenotype
Báo cáo y học: "Genome-wide promoter extraction and analysis in human, mouse, and ra" potx
Ngày tải lên :
14/08/2014, 14:22
... located either in the gene region or in the 20 kb upstream of the gene (if the gene has RefSeq mRNA, the distance was limited to kb), and these promoters/TSSs were collected as 'gene- related promoters/TSSs' ... boundary The UniGene ID was linked to the gene on the basis of its transcripts For genes with Ensembl transcript ID, using the information from Ensembl's EnsMart, we marked the orthologous gene sets ... Materials and methods) In both sets, we shortened each gene by kb (or half of the gene length if the gene is shorter than kb) from its 5' end to simulate 5' incomplete genes that are most common in the...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
Ngày tải lên :
31/03/2014, 07:20
... two bands The slower migrating band always corresponded to the rat brain control, and the faster migrating band to the N18TG2 control (Fig 5) The existence of both rat and mouse VRL-1 in the same ... rVRL-1-GFP band appeared at 110 kDa, the upper band at 114 kDa and the diffuse band at 128 kDa The endogenous VRL-1 can also be observed at 80 kDa in the anti-VRL-1 Western blot and does not ... TTT] and amino acid 462 [1713 TTT fi TTC] These differences lead to two changes in the amino-acid sequence in VRL-1, namely S82R and P367L The other two differences are silent To obtain the full...
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Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt
Ngày tải lên :
21/02/2014, 00:20
... provided by the Harvard Brain Tissue Resource Center (Belmont, MA, USA) and the Netherlands Brain Bank (Amsterdam, The Netherlands) Blocks of cortex, anterior amygdala, striatum and midbrain ... Adh4 mRNA in the stomach epithelia – the tissue with the best characterized Adh4 expression Taken together, our results demonstrate expression of ADH3 in most of the analyzed areas in the brain, ... expression levels were found in mouse (Fig 1C) and human liver (Fig 2B) and moderate expression levels in rat liver (Fig 1A), in accordance with the literature [16–18] The difference in the expression...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
Ngày tải lên :
16/03/2014, 16:20
... weakly with the reagents currently available To analyse further the composition of the PS1 complex, samples with high expression of PS1 complex were selected for further study BN/PAGE gel bands corresponding ... tissue and from the neuroblastoma line SH-SY5Y (SY5Y) Experimental procedures Antibodies Synthetic peptides derived from human sequences were conjugated to diphtheria toxoid and rabbit antibodies generated ... and role of the PS complex components other than PS We have applied Blue Native gel electrophoresis (BN/PAGE) [19] to analyse further and characterize endogenous native complexes from mouse and...
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báo cáo hóa học:" Tissue specific characteristics of cells isolated from human and rat tendons and ligaments" pptx
Ngày tải lên :
20/06/2014, 01:20
... (Tables and 2) Interestingly in both the rat and the human patellar samples, the marker CD90 showed a higher intensity of expression than in the ACL (human) or Achilles and tail (rat) In the rat there ... was then removed by washing under running tap water The cultures were dried and photographed, then analyzed using "Gene Tools" image analysis software (Syngene, Cambridge, UK) and the number and ... were no other obvious differences in the markers tested In the human samples, the levels of expression varied greatly between patients, however, the general pattern of expression remained the same...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
Ngày tải lên :
12/08/2014, 04:21
... hCD4 expression in the CCRT K4 clone, most likely due to the silencing effect of chromatin on the integrated promoter, we enhanced the expression of the receptors by incubating the cell with the ... sequence and another primer targeting the sequence of HIV-1 MN LTR The indicated band (arrow) was isolated, cloned and its sequence (GenBank AY703985) confirmed the integration of HIV-1 into the cotton ... HIV-1 stocks was in the order of the ng/ml and followed kinetics compatible with viral production In addition we showed that the expression of the receptors on the cells determines the viral type...
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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
Ngày tải lên :
12/08/2014, 04:21
... hCD4 expression in the CCRT K4 clone, most likely due to the silencing effect of chromatin on the integrated promoter, we enhanced the expression of the receptors by incubating the cell with the ... sequence and another primer targeting the sequence of HIV-1 MN LTR The indicated band (arrow) was isolated, cloned and its sequence (GenBank AY703985) confirmed the integration of HIV-1 into the cotton ... HIV-1 stocks was in the order of the ng/ml and followed kinetics compatible with viral production In addition we showed that the expression of the receptors on the cells determines the viral type...
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Báo cáo y học: " Biphasic effect of extracellular ATP on human and rat airways is due to multiple P2 purinoceptor activation" ppsx
Ngày tải lên :
12/08/2014, 18:20
... experiments and [Ca2+]i recording on isolated cells, participated in the analysis of the data, and helped the draft of the manuscript RM participated in the design of the study and helped the draft of the ... indicated in the text In epithelium-free experiments, the epithelium of isolated rings was rubbed using a plastic cylinder introduced in the lumen of the ring Rings were frozen at the end of the experiment ... day-cultured cells, the amplitude of the [Ca2+]i peak reincreased up to the values observed in non-cultured myocytes, and the general profile of the response dramatically altered, as shown in the original...
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Báo cáo y học: " Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages" docx
Ngày tải lên :
12/08/2014, 23:20
... activity or the amount of p24 was calculated, and the value of the mock sample was normalized to Values are means of triplicate samples and the SD was calculated The amount of p24 in the FPM1 and C58(NT)D ... which expresses the luciferase gene driven by http://www.retrovirology.com/content/6/1/43 the HIV-1 LTR, to examine the effect of hCycT1 and hCRM1 on Tat-directed gene expression Expression of ... expressed hCycT1 and hCRM1, and then quantified the production of p24 Again, hCycT1 greatly augmented p24 production, and hCRM1 had a moderate effect Notably, the levels of hCycT1 and hCRM1 expression...
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Báo cáo y học: "TFCat: the curated catalog of mouse and human transcription factors" pdf
Ngày tải lên :
14/08/2014, 21:20
... of these homologous TF genes were in the UPTF set Inspection of the 20 putative mouse homologs of fly TFs absent from the UPTF set led to the inclusion of genes in both the UPTF and the TFC sets ... selected and subsequently mapped to Entrez Gene using the Gene2 Refseq table Standardizing TF gene candidate annotation A website annotation tool and MySQL database were developed to standardize and ... devised and implemented the homolog analysis and gene clustering process DLF, SS, and RS worked on the wiki gene page format DLF designed, developed and implemented the wiki DLF developed and implemented...
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Investigation of relative expression level of SLC4 bicarbonate transporter family in mouse and human corneal endothelial cells
Ngày tải lên :
13/10/2015, 15:54
... of the choroid, the ciliary body, the iris and the innermost is the retina which sends neural signals to the brain through the optic nerve Within these coats lie the aqueous humor, the lens and ... to the ciliary body by the suspensory ligament The vitreous body fills the posterior chamber bordered by the sclera and the lens Figure 1.1 Structure of the eye 1.1.1 The cornea The cornea, the ... that there is the water balance between the corneal stroma and the aqueous humour caused by the leak of aqueous fluid into the stroma and the pump that moves fluid out of the stroma The corneal...
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Báo cáo y học: "Human toxoplasmosis and the role of veterinary clinicians"
Ngày tải lên :
03/11/2012, 11:06
... women in Korea J Parasitol 2008; 94: 963-965 Grant S and Olsen CW Preventing zoonotic diseases in immunocompromised persons: the role of physicians and veterinarians Emerg Infect Dis 1999; 5: 159-163 ... among persons infected with the human immunodeficiency virus Clin Infect Dis.1994;18:14-24 Han K, Shin DW, Lee TY, et al Seroprevalence of Toxoplasma gondii infection and risk factors associated ... 134 aged This is particularly true with regards to T gondii, where the consequences of infection can be very severe, while the risk of living with one’s beloved cat is practically nill References...
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Human rationality and artificial intelligence
Ngày tải lên :
01/11/2013, 07:20
... keyboard and printer on one side and another keyboard and printer on the other side By means of these devices you can send and receive typewritten messages to and from the occupants of the two ... premises of the form ‘If P, then Q’ and ‘Not Q’, people first of all adopt a hypothesis of the form ‘P’, then apply the rule of modus ponens to ‘If P, then Q’ and ‘P’ to get ‘Q’, and finally infer ... help much to alleviate the air of paradox On the other hand, it is not entirely clear what real substance there is to the rival approach of the mental models theorists.11 For the very process of...
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The Modern Human colonisation and the Neanderthal extinction
Ngày tải lên :
01/11/2013, 07:20
... restricted to the Mediterranean peninsulas (and Crimea and the Caucasus) during the colder episodes Neanderthals therefore were restricted to southern refugia during cold and arid events and they were ... caused the extinction of the Neanderthals The only basis for the argument is that of an apparent association between the arrival of the Moderns into Europe and the extinction of the Neanderthals ... habitats and topographically heterogeneous zones In the latter case the mid latitude belt from Portugal and the Maghreb to the Caucasus and the Altai would have presented a large mass of heterogeneous...
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The revolution in philosophy (I) - human spontaneity and the natural order
Ngày tải lên :
01/11/2013, 08:20
... picture therefore, we have on the one hand the identity of the thinking subject, and on the other hand the multiplicity of the representations which it has The same complex thinking subject – as the ... make There are two ways, Kant suggested, that we can look at judgments: on the one hand, we can regard the form of the judgment (how the subject is related to the predicate); and, on the other hand, ... heavily informed by the discussion in Michael Friedman, Kant and the Exact Sciences, who has one of the most detailed and informative discussions of the issues Part I Kant and the revolution in...
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The return of universalist law - human rights and free trade
Ngày tải lên :
01/11/2013, 08:20
... in the Roman Empire and Greece in the time of Alexander the Great.19 The hegemonic status of the US in European developments and the quest for order following the world wars warrants some theoretical ... Mammon Empire? These successors were the king, the state, the people and the state organization (the League of Nations, the United Nations etc.) The state is then constructed in the image of ... facilitate the expansion and balanced growth of international trade, and to contribute thereby to the promotion and maintenance of high levels of employment and real income and to the development of the...
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Tài liệu To establish an Office on Women’s Health within the Department of Health and Human Services, and for other purposes pdf
Ngày tải lên :
13/02/2014, 07:20
... publications and any other means appropriate, provide for the exchange of information between the Office and recipients of grants, con- tracts, and agreements under subsection (c), and be- tween the Office ... professionals, and other individuals 16 and groups, as appropriate, on the policy of the Cen- 17 ters with regard to women; and 18 ‘‘(5) serve as a member of the Department of 19 Health and Human ... COMPOSITION. The Coordinating Com- 14 mittee shall be composed of the Directors of the 15 Centers and Offices 16 ‘‘(3) CHAIRPERSON. The Director of the Of- 17 fice shall serve as the chairperson of the...
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Tài liệu NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di-Isodecyl Phthalate (DIDP) pdf
Ngày tải lên :
13/02/2014, 10:20
... 11, and 1,000 mg/kg, and the percentage of the oxidative derivative of the monoester and of MIDP at the same doses were, respectively, 25 and 30%, 14 and 26%, and 13 and 13% The data suggest a ... estimated from the total radioactivity excreted in urine and bile or retained in the carcass at the end of 72 hours, and were 56, 46, and 17% for the low, medium, and high doses, respectively The remainder ... Next, the CERHR prepares the NTP-CERHR monograph The NTP-CERHR monograph includes the NTP brief on the chemical evaluated, the expert panel report, and all public comments The goal of the NTP...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf
Ngày tải lên :
16/02/2014, 14:20
... 3-kinase and Hedgehog, and thus affects metabolism, the cell cycle, gene expression, developmental processes and oncogenesis Active GSK3 is indicative of absent WNT-signaling and leads to the proteasomal ... deregulated HoxA genes, for example, HoxA7 and HoxA9 The transforming capacity of the tested MLL fusion constructs was also dependent on the presence of Meis1 and Pbx proteins, as well as on the presence ... MLL–AF4 Short-term protein expression of MLL–AF4 in a doxycycline-dependent manner resulted in the ectopic activation of HoxA7 and HoxA10 (and 560 other genes), whereas the reciprocal AF4–MLL fusion...
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