... submitted to MALDI-TOF analysis and assigned to distinct subunits.Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin.About 10 lg of total hemolymph protein (HL) and 3 lgofpurifiedhemocyanin ... were used for the analyses of DNA and amino acid sequences. Signal peptides were predictedusing the online version of SIGNALPV1.1 [28]. The aminoacid sequencesof the S. coleoptrata hemocyanins ... subunits and HcX, plus 6–45 bp of the respective 5Â untranslatedregions and the entire 3Â untranslated regions. The standardpolyadenylation signals (AATAAA) and the poly(A)-tails of different...
... of A. vinelandii ferric reductase, the enzymewas purified as a mixture of two proteins or a hetero-dimer with molecular masses of 44 600 and 69 000 Da[25]. Although none of the amino acid sequences ... theKm and kcatvalues of E. coli Fre for riboflavin arereported as 2.5 lm and 52.4 s)1with NADPH as theelectron donor and 1.3 lm and 30.6 s)1with NADH[13]. For FMN, Km and kcatvalues of ... twofold axis associated with thedimer passes through the vicinity of the side chains of Pro13, Ile126, Gln133 and Ile163. The correspondingTable 1. Summary of x-ray data collection, phasing and...
... better defined set of fitting parameters.The adequacy of the analyses was determined from thereduced weighted sum of squares of residuals, and visualinspection of the distribution of weighted residuals. ... global and localdynamics of the AEDANS and W94 probes in free and DNA-bound RepA. The decay of the total fluores-cence intensity was recorded, as well as the decays of its vertically and horizontally ... each of the sequences (Fig. 3D–F), indicating adecrease in the rotational mobility of AEDANS C160–RepA. The anisotropy increase is slightly different foreach of the three sequences, and relates...
... represent the intensity of each band normalized to theband in lane 1. The lower numbers represent the intensity of the bandsin lanes 5 and 6 normalized to the band in lane 4.1798 I. Kotova et ... mutation of it toGCGCGC (Fig. 3D; G-less templates TATAWtUTRmut and TATAmutUTRmut). We found that both TBP (Fig. 2A,compare lanes 4 and 5) and the R2 TATA-box (Fig. 2A,compare lanes 2 and ... regulation of expression of the ribonucleotide reductase R2 subunit arefocused on sequences located upstream from the R2transcription start site, and on regulatory proteins that bindto these sequences. ...
... proper-ties of these two types of HSA. We investigated thebinding of L(small)-Trp as an endogenous ligandwhich binds to site II, andof cefazolin (site I-ligand) and verapamil (site I and II-ligand) ... frag-ment of HSA. N, nonoxidized HSA; H, highly oxidized HSA. (B)Densitometric analysis of intensities of undigested HSA. Changesin intensities of HSA bands relative to the band of starting point of digestion ... towards l-Trp of HSA. Thismight also be related to increased levels of serotonin and the frequent occurrence of some adverse complica-tions of diseases which have an increased level of oxid-ized...
... Nystrom3 and Peter Stralfors11 Department of Cell Biology and Diabetes Research Centre, University of Linkoăping, Sweden2 Department of Molecular and Clinical Medicine, University of Linkoăping, ... compilation ª 2006 FEBS 3383 Separation and characterization of caveolae subclasses inthe plasma membrane of primary adipocytes; segregation of specic proteins and functions Unn Oărtegren1, Lan ... separation and biochemical char-acterization of three classes of caveolae, which demon-strate a segregation offunctions in caveolae subclasses.ResultsPrimary rat adipocytes were homogenized and...
... theprobabilities of the rules VYr→ y and V¯Yr→ 1−yto 1 and V¯Yr→ y and VYr→ 1 − y to 0. The rest of the weights for Sφj→ Sφj−1Ajare set to 1.This assignment of rule probabilities ... appearance of both rules of theform VYr→ 0 and VYr→ 1 cannot occur be-cause all rules that appear in the Viterbi parsetree have probability 1.2. For any r, an appearance of rules of the ... set-tings (Yejin and Cardie, 2007; Wang et al., 2007;Goldwater and Johnson, 2005; DeNero and Klein,2008; Spitkovsky et al., 2010). In the context of PCFGs, it aims to select parameters θ and phrase-structure...
... from the sequencesof V. a. zin-nikeri and neurotoxic V. a. aspis (DDBJ/EMBL/GenBank accessionno.s AY152843 and AF548351) and that of vaspin chain B, from the sequences of V. a. zinnikeri and neurotoxic ... ammo-dytins I1 and I2 and ammodytin L genes of V. am. ammo-dytes, the VP7 and VP8 genes of V. palaestinae,andthePLA2 genes of Crotalinae snakes (Trimeresurus flavoviridis)were also of similar length ... promoters.Analysis of venom gland PLA2 cDNAs is currentlyunderway to determine which of these genes are expressed.The presence in the neurotoxic snake of two ammodytinI1 isoforms (In and Ia) andof several...
... crystal.Fig. 5A and B shows scanning electron microscope images of a typical sample of fusiform ␣-Fe2O3nanocrystallines obtainedat 140◦C for 24 h and indicate the large quantity and gooduniformity ... Interface Sci. 254(2002) 87. Journal of Alloys and Compounds 433 (2007) 216–220Hydrothermal synthesis and characterization of ␣-FeOOH and ␣-Fe2O3 uniform nanocrystallinesXiaohe Liua,b, ... con-trolled size and shape have attracted vast attention because of their size and shape-dependent properties and great potentialapplications. Considerable effort has been devoted to the design and controlled...
... points and my colleagues and friends E. Kowalski and J. Vanderkam with whom I began a fairly ex-tensive study of Rankin-Selberg L -functions. I also thank the referee for histhorough review of the ... cos(2πnx)K0(2πny)or Eisenstein seriesof weight one. In spite of this analogy, and the fact that ourproof borrows some material and ideas from [DFI8], we wish to insist that thebulk of our approach requires ... Cauchy-Schwarz and Hθ. From (2.31), the last inequality and (2.32) weconclude the proof of Proposition 2.3.3. Rankin-Selberg L -functions Our basic reference for Rankin-Selberg L -functions is the book of...
... the formation of aG-quadruplex and i-motif upstream of the Bcl-2 P1promoter prevents the binding of WT-1 and abro-gates the transcriptional repression, thereby allowingactivation of Bcl-2 transcription. ... MINIREVIEWMaking sense of G-quadruplex and i-motif functions inoncogene promotersTracy A. Brooks1,2,3, Samantha Kendrick3 and Laurence Hurley1,2,31 University of Arizona, College of Pharmacy, Tucson, ... the increasingly observed complexity of G-quadruplex (G-rich strand) and i-motif (C-richstrand) folding patterns and structures in the promoterregions of oncogenes. Accompanying minireviews...
... in me), to the firm conviction of the existence of God andof theimmortality of the soul. In my Journal I wrote that whilst standing in the midst of the grandeur of a Brazilianforest, 'it ... shores of South America of the intermittent elevation of the land, together with denudation and the deposition of sediment. This necessarily led me to reflect much onthe effects of subsidence, and ... birch,privet, and dogwood, and with a long line of hollies all down the exposed side. In earlier times he took acertain number of turns every day, and used to count them by means of a heap of flints,...
... Platination of telomeric sequencesand nucleasehypersensitive elements of human c-myc and PDGF-Apromoters and their ability to form G-quadruplexesViktor ViglaskyDepartment of Biochemistry, Faculty of ... with G-rich sequences is vast; in principle,there is the possibility to bind within each guanine, toform intrastrand and interstrand bifunctional and monofunctional adducts, etc. Some of the proposedbinding ... ChottardJC (2003) Platinum cross-linking of adenines and gua-nines on the quadruplex structures of the AG3(T2AG3)3 and (T2AG3)4 human telomere sequences in Na+ and K+solutions. Nucleic Acids Res...
... understanding of thestructure and evolution of proteins. It will play animportant role in the interpretation of the sequences produced by the genome projects and, therefore, inunderstanding ... Classification of Proteins Databasefor the Investigation ofSequencesand StructuresAlexey G. Murzin, Steven E. Brenner, Tim Hubbard and Cyrus Chothia*MRC Laboratory of Molecular To facilitate understanding ... (3) alpha and beta (for proteins witha-helices and b-strands that are largely inter-spersed), (4) alpha plus beta (for those in whicha-helices and b-strands are largely segregated) and (5) multi-domain(forthose...
... KOZMA AND ALEXANDER OLEVSKI˘ı[H78] S. V. Hruˇsˇcev, The problem of simultaneous approximation andof removal of thesingularities of Cauchy type integrals (Russian), in Spectral Theory ofFunctions ... scale of smoothness connecting C∞ and analyticity, this Annals of Mathematics Analytic representation of functions and a new quasi-analyticity threshold By Gady Kozma and Alexander ... immediately gives (85) and proves the lemma. Annals of Mathematics, 164 (2006), 1033–1064Analytic representation offunctions and a new quasi-analyticity thresholdBy Gady Kozma and Alexander Olevski˘ı*AbstractWe...