sequence characteristics of plant gads

... from non -plant species 154 31 Alignment of nucleotide sequence of the 5’- and 3’-termini of mustard GADs 156 32 Prediction of the secondary structure of C-terminal of mGADs with PSIPRED software ... acid sequences of mGAD2 149 29 Comparison of the deduced amino acid sequence between mGADs and other plant GAD homologs 152 30 Comparison of the deduced amino acid sequence between mGAD2 and GADs ... 2.3 Effects of PAs on oxidative stress 39 2.4 GABA shunt pathway 41 2.4.1 GAD 43 2.4.1.1 Sequence characteristics of plant GADs 43 2.4.1.2 GAD expression 44 2.4.1.3 Regulation of plant GADs 46 2.4.2...

Ngày tải lên: 12/09/2015, 10:19

... of desired product PCR: make large amounts of a particular sequence • The number of molecules of the DNA fragment between the primers increases about 2-fold with each cycle • For n = number of ... Polymerase Chain Reaction • Requires knowledge of the DNA sequence in the region of interest • As more sequence information becomes available, the uses of PCR expand • With appropriate primers, one ... vector: pUC lacZ mulitple cloning sites pUC ApR ColE1 origin of replication Lac+, or blue colonies on X-gal in appropriate strains of E coli High copy number foreign DNA lacZ pUC recombinant ApR...

Ngày tải lên: 13/03/2014, 18:39

... best hit of each sequence of cassava in each of the other databases and the best hit of each sequence of the other species in cassava We thus built a table representing the network of best hits ... number of expressed sequences from cassava already present in GenBank, we conducted a BLASTN search of the sequences in our assembly against the 36162 EST sequences in dbEST as of April 2007 Any sequence ... http://www.biomedcentral.com/1471-2229/7/66 Figure profile of the assembly of cassava ESTs Cluster Cluster profile of the assembly of cassava ESTs The graph presents the number of clones per assembled scaffold;...

Ngày tải lên: 12/08/2014, 05:20

... lack of standardized criteria for laboratory diagnostics of chronic C pneumoniae infection as well as contradictory information about distribution of the pathogen throughout of the tissues of human ... µl of DNA was added to reaction mixture containing 1x PCR buffer (Silex, Moscow, RF) containing 10 mM Tris-HCl, pH 8,3 , 2.5 mM MgCl2, 15 pmol of each primer, 200 µM of each of dNTPs and U of ... diagnostics of C pneumoniae infection (8, 9) However, changed antigenic profile of C pneumoniae during persistent colonization in human tissues (10, 11) undermines the diagnostic value of serological...

Ngày tải lên: 26/10/2012, 08:57

... bars at the top of the sequences The sequences similar to HRT1 are shaded Identical amino acid residues in greater than four of the seven sequences are boxed nucleotide sequence of the fragment ... Biochem 270) 4675 Fig Comparison of the deduced amino acid sequences of HRT1 and HRT2 The deduced amino acid sequences of HRT1 and HRT2 are compared with those of cis-prenyltransferases from A ... those of the conserved regions II, III and IV of cis-prenyltransferase was designated as LT600 The Fig Nucleotide and deduced amino acid sequences of HRT1 (A) and HRT2 (B) Numbers of nucleotide sequence...

Ngày tải lên: 07/03/2014, 21:20

... revealed the amounts of RNA loaded per lane The sizes of an RNA marker are indicated the cloning of the 5¢ and 3¢ end of the clone, respectively Analysis of the nucleotide sequences of the newly obtained ... J Biochem 269) 4603 Fig Protein sequences of PPOD1 and PPOD2 (A) Sequence comparison between the deduced amino acids of the two obtained clones The sequences of the originally obtained tryptic ... and then sequenced with an automated sequencer The amino acid sequences of four peptides (Table 1), derived from the puri8 fied protein, were not present in the SWISS PROT database Cloning of the...

Ngày tải lên: 08/03/2014, 10:20

... comparison, bacterial ribosomal proteins of L9 family were shown below the sequence alignment of Ynr022c (MrpL50) with its homologue Boxes show degrees of sequence conservation with asterisks indicating ... medium and showed slow growth on YPD as in the case of disruptants of most other mrp genes In the case of MRPS8, it was indicated that the addition of a short peptide to the C-terminus caused poor ... synthesis lead to the loss of mitochondrial genome Recent analysis of mutants indicated that availability of isoleucine in the cell might be related to the stability of the mitochondrial genome...

Ngày tải lên: 17/03/2014, 10:20

... yield of the apo-BCCPD67 K117L mutant was  15 mg of protein per litre of cell culture Biochemical properties of BPL Activity assays were performed with BPL by measuring the incorporation of [14C]biotin ... formation of a fragment of  21 kDa Chymotrypsin digestion also produced an array of smaller peptide fragments We found that only 34% of total BPL remained after trypsin cleavage in the absence of substrates ... of 37 kDa (Fig 6, inset) Electrospray analysis of the complex gave a molecular mass of 37 200 ± 200 Da which agrees well with the predicted mass of a : heterodimer Discussion The attachment of...

Ngày tải lên: 31/03/2014, 07:20

... little homology of sequence (a) between the three active groups of peptides shown in Table 1, (b) with the sequence of amino acid residues of the Ca2+/calmodulin binding domain of nNOS, (c) with ... structures of certain domains of nitric oxide synthase isoforms are known [53±57], we are not aware of the three-dimensional structure of the calmodulin binding sites of any isoform being reported Thus, ... 82±101 of caveolin-1 in the form of glutathione S-transferase fusion proteins have been shown to attenuate the activities of all three isoforms of NOS [62] A novel 10-kDa protein inhibitor of nNOS,...

Ngày tải lên: 31/03/2014, 15:20

... the roof If additional roof deflection is mm or less, the isolator should be selected for 10 times the additional roof deflection If additional roof deflection is over mm, supplemental roof stiffening ... class of equipment Certain spaces may dictate higher levels of isolation For example, bar joist roofs may require a static deflection of 38 mm over factories, but 64 mm over commercial office ... class of equipment Certain spaces may dictate higher levels of isolation For example, bar joist roofs may require a static deflection of 38 mm over factories, but 64 mm over commercial office...

Ngày tải lên: 31/03/2014, 18:20

... concentrations of relevant RNAs by drug-induced overexpression of genes of interest, and (3) inhibition of protein synthesis by inhibitors, resulting in extended transcription of the early genes of mammalian ... significance of these molecules In addition, one can examine differential gene expression of other genes in the presence and absence of the gene of interest by modulating the levels of each gene of interest ... successfulness of 5′-RACE relies on the incorporation of the CapFinder Adaptor sequence at the beginning of the cDNA As mentioned, this step depends on the addition of extra oligo(dC) at the end of the...

Ngày tải lên: 11/04/2014, 01:04

... 20% The direct costs of the production of m3 of raw wood assortments were derived by means of the product of SH/m3 with the wage schedule and coefficient of recalculation of labour costs to direct ... difference of gross yield and own costs of logging Value yield tables of poplar clones were constructed by the method of simulation from the models of assortment yield tables, prices of the assortments ... fusion of models of yield tables and stand assortment tables, models of quality and models of damage to stands Models of yield tables (Petráš, Mecko 2001, 2005) determine the development of mean...

Ngày tải lên: 07/08/2014, 03:22

... the evaluation of three times four plants The growth traits of stem diameter at a height of 0.5 m to the nearest mm and plant height to the nearest 0.1 m were measured at the end of the growing ... the time frame of the main incidence of the rust Stem diameter and the main part of plant height were realized before the onset of the pathogen incidence while the production of storage compounds ... from clones 7, 107, 110, 152 The stability of the intensity of infection of black poplar clones by the rust (the rank of clones according to the intensity of infection) between the particular years...

Ngày tải lên: 07/08/2014, 10:21

... diameter of stand (cm) Fig Proportions of the quality classes of logs of clone Robusta with zero and 40% damage of stems of quality class A Fig Proportions of the quality classes of logs of clone ... classes of logs of clone Robusta with zero and 40% damage of stems of quality class B Fig Proportions of the quality classes of logs of clone I-214 with zero and 40% damage of stems of quality ... measurement of quantitative parameters The introduction of further stand characteristics, e.g range of tree diameters, mean height, kind of damage to stems, age of damaged stems, quality of site,...

Ngày tải lên: 07/08/2014, 10:21

... with ADOL-Hc1, suggests that some sequences of this isolate in E element or LTR regions may be deleted In order to characterize which part of gene sequences of Korean isolates is deleted, further ... of the HPRS-103 strain of J subgroup avian leukosis virus and of a derivative acutely transforming virus Vet Pathol 1997, 34, 127-137 Bai, J., K How es, L N P ayne, and M A Skinner Sequence of ... that ten of twelve isolates were not neutralized by antibodies to any of ALV subgroups including J , and only two isolates were neutralized with a specific serum of HPRS-103, the prototype of subgroup...

Ngày tải lên: 07/08/2014, 15:20

... In vitro cholesterol-lowering test: Among isolated 100 strains of LAB, of Streptococcus strains, 11 of Lactobacillus strains and of Bifidobacterium strains were selected as provisional probiotic ... vande Woestyne M, Verstraete W In vitro study of bile-salt hydrolase (BSH) activity of BSH isogenic Lactobacillus plantarum 80 strains and estimation of cholesterol lowering through enhanced BSH ... presence of bile salt These results showed that many LAB have a great cholesterol-lowering activity in the MRS broth regardless to the presence of bile salt (0.3% oxgall) (Table 2) Identification of...

Ngày tải lên: 07/08/2014, 18:20

... individual clones The influence of the genetic background of the mother tree is demonstrated by differences found in plant survival by the mean number of surviving plants/clone (table I) Depending ... this article, we report the micropropagation of late-flushing Q robur from acorns of selected trees To our knowledge, results of ex vitro rooting of micropropagated oak are presented here for ... sterile water times for In each case, half of the embryos of the 11 mother trees were placed on solid Gresshoff and Doy (GD) medium (1972) and woody plant medium (WPM) (Lloyd and McCown, 1980),...

Ngày tải lên: 08/08/2014, 19:21

... (1986) Sources of variation and measures of variability in even-aged stands of plants Ann Bot 58, 757-778 Bisoffi S (1988) Border effects in a multiclonal poplar (Populus spp) plantation Genet ... clonal block planted At the location of Orsay (48°42’N, 02°12’E, Paris at about 280 km SSW of Afsnee) in France, another SRIC plantation was established at the same time in blocks of cuttings ... development of each individual within a particular stand This development may be influenced by other factors, eg, the availability of limiting resources This may be the origin of size hierarchies of individuals...

Ngày tải lên: 08/08/2014, 19:21

... characteristics was collected on representative trees of each of the clones Number of branches, branch length and diameter, and the angles of origin and termination ... measured Dry weight (DW) of proleptic and sylleptic branches, current terminal and the remainder of the stem were determined, as well as DW and leaf area (LA) of leaves of current terminal, proleptic ... the middle of the growing season, 35-40% of the total LA were carried on sylleptic branches in hybrid clones 44-136 and 11-11, respectively The LA on the current terminal of each of the clones...

Ngày tải lên: 09/08/2014, 02:21

... (Promega) and sequenced with a universal sequencing kit (Perkin Elmer) The sequencing products were run on an ABI377 sequencer The resulting sequences were compared to existing sequences using ... Hors-Cayla [9] and is composed of a total of 38 hamster-bovine cell lines A more complete description of the panel is given in Laurent et al [11] A correlation coefcient of 0.69 was used as the threshold ... hybridization mapping of LDHA and IGF2 to cattle chromosome 29, Mamm Genome (1996) 473 200 M Gautier et al [16] Wilcox S., Khan A.S., Hopkins J.A., Sikela J.M., Use of untranslated sequences of human cDNAs...

Ngày tải lên: 09/08/2014, 18:21