selecting a mouse model of alzheimer s disease

... traditional image analysis approach Thus, this method is shown to accurately assess amyloid burden in transgenic mouse models of AD In addition, the use of object-based analysis provides data ... plasma A beta clearance and decreases brain A beta burden in a mouse model of Alzheimer' s disease Proc Natl Acad Sci U S A 2001, 98:8850-8855 Bacskai BJ, Kajdasz ST, McLellan ME, Games D, Seubert ... humans, particularly at that stage of the disease when substantial fibrillar amyloid deposits have begun to accumulate [10] It is this stage of the disease that often correlates with appearance of...

Ngày tải lên: 19/06/2014, 22:20

... significant changes as a consequence of a- synuclein overexpression or as a result of the association of dopamine treatment with a- synuclein overexpression (Fig 3B) The same analysis performed on all ... Pro transmission scanner (Epson, Nagano, Japan) and analyzed with imagemaster 2d platinum software, version 5.0 (GE Healthcare) Spots were detected automatically by the software and manually refined; ... experimental parkinsonism Proc Natl Acad Sci USA 104, 1977–1982 37 Werner CJ, Heyny-von Haussen R, Mall G & Wolf S (2008) Proteome analysis of human substantia nigra in Parkinson s disease Proteome Sci...

Ngày tải lên: 15/02/2014, 01:20

... isoforms, whereas MAP 1A and MAP1B sequential proteolysis results from caspase and calpain activation This work confirms the cross-talk between caspase and calpain and identifies a novel mechanism associated ... Although calpains may enhance caspase activity, they can also function to block the activation of caspases For example, calpains can cleave caspase rendering it incapable of activating caspase and ... possible cascade of events involving three protease systems: calpain-induced cathepsin release, cathepsin-mediated caspase activation and caspase-mediated calpastatin degradation leading to enhancement...

Ngày tải lên: 23/03/2014, 10:21

... level of TNFalpha and bax mRNA in the striatum and induces apoptosis of dopaminergic neurons in hemiparkinsonian rats Brain Res 2004, 996:237-245 Adams FS, LaRosa FG, Kumar S, Edwards-Prasad J, ... model of PD We used the striatal administration of 6-OHDA because neuronal damage and death, characteristically progress over weeks This provides a close (although accelerated) model for the cascade ... 28:439-453 Isacson O, Brundin P, Gage FH, Bjorklund A: Neural grafting in a rat model of Huntington 's disease: Progressive neurochemical changes after neostriatal ibotenate lesions and striatal tissue...

Ngày tải lên: 19/06/2014, 22:20

... manufacturers protocol Statistical analyses For statistical analyses, ANOVA and t-tests were performed where appropriate using SPSS for Windows release 10.1 Hierarchical cluster analysis of A -cytokine ... statistical analyses and aided in manuscript preparation VM analyzed data and constructed dendrograms AQ aided in ELISA and Bio-plex assays and collected mouse brain tissues FC oversees management of ... There are numerous reports of increased levels of cytokines in the brains of Alzheimer' s disease patients, and in transgenic mouse models of Alzheimer' s disease [10-12] However, all these reports...

Ngày tải lên: 19/06/2014, 22:20

... Three animals per group were analyzed and each sample was analyzed in duplicate Statistical analysis Statistical analysis of the data was performed using GraphPad Prism version 5.00 for Windows (GraphPad ... Neurodegenerative Diseases & key Laboratory of Stem Cell Biology, Institute of Health Science, Shanghai Institutes of Biological Sciences (SIBS), Chinese Academy of Science (CAS) & Shanghai Jiao Tong ... 6-OHDA/Veh and 6-OHDA/CAb groups (n = per group) Results are expressed as mean ± S. E.M (ng/g WW) of total protein Data are shown only for ipsilateral (right) side of striatum Statistical analysis...

Ngày tải lên: 19/06/2014, 22:20

... Novartis Pharma) daily for immunosuppressive Computer-assisted method for gait analysis Rats were subjected to gait assessment with the CatWalkautomated gait analysis system (Noldus Information ... avoid unsteadiness and falling during walking In conclusion, the CatWalk-assisted automated gait analysis system revealed that unilateral infusion of 6-OHDA leads to functional changes in static ... disorders, such as Parkinson 's disease and Alzheimer' s disease [15] Neural stem cells that have been transplanted into 6-OHDA-lesioned rats have been shown to differentiate into neuronal cell types...

Ngày tải lên: 10/08/2014, 05:21

... Ceruloplasmin is increased in cerebrospinal fluid in Alzheimer s disease but not Parkinson s disease Alzheimer Dis Assoc Disord 8, 190–197 Hye A, Lynham S, Thambisetty M, Causevic M, Campbell J, Byers ... kinases and phosphatases [3] One particular tau kinase pertinent to metal dyshomeostasis in AD is glycogen synthase kinase-3 (GSK3) GSK3 has recently been implicated as a critical kinase involved ... Sci USA 83, 4913–4917 Alonso AC, Grundke-Iqbal I & Iqbal K (1996) Alzheimer s disease hyperphosphorylated tau sequesters normal tau into tangles of filaments and disassembles microtubules Nat Med...

Ngày tải lên: 07/03/2014, 10:20

... image, as previously described [9,16,17] Analyses were always performed in a coded fashion Statistical analysis Data are expressed as mean ± standard error of mean (S. E.M.), analyzed by analysis ... G, Aliev G, Irai K, Takeda A, Balraj EK, Jones PK, Ghanbari H, Wataya T, Shimohana S, Chiba S, Atwood CS, Petersen RB, Smith MA: Oxidative stress is the earliest event in Alzheimer' s disease ... Fibrillary β-amyloid evokes oxidative damage in a transgenic mouse model of Alzheimer' s disease Neuroscience 2001, 104:609-613 Stewart WF, Kawas C, Corrada M, Metter EJ: Risk of Alzheimer' s disease and...

Ngày tải lên: 19/06/2014, 22:20

... versus rotation speed [23] Statistical analysis All data were analyzed using an IBM-based personal computer statistical package (SYSTAT 10, SPSS Inc, Chicago, IL) Except for the correlation analyses, ... Etminan M, Gill S, Samii A: Effect of non-steroidal anti-inflammatory drugs on risk of Alzheimer' s disease: systematic review and meta-analysis of observational studies Bmj 2003, 327:128 Page 15 of ... 358:351-359 Shiozaki S, Ichikawa S, Nakamura J, Kitamura S, Yamada K, Kuwana Y: Actions of adenosine A2 A receptor antagonist KW-6002 on drug-induced catalepsy and hypokinesia caused by reserpine...

Ngày tải lên: 19/06/2014, 22:20

... visual analysis of scans, describes how quantification with clinically available and friendly software tools can be employed to assist with analysis, and then illustrates a straightforward approach ... important to accurately diagnose dementia as early as possible This chapter outlines the basic elements of a clinical diagnostic evaluation for Alzheimer s disease (AD) and other dementias; and also ... more severe and global as AD progresses Behavioral and personality changes, including agitation, aggression, and psychosis, often emerge as the disease progresses Depressed mood often occurs with...

Ngày tải lên: 05/03/2014, 23:20

... of persons without the disease who are accurately diagnosed as not having the disease) , and what is meant by disease. ” As we go to press, AD remains a clinical diagnosis which for years has been ... disease Frontotemporal and Parkinsonian dementias Huntington s disease Neuronal ceroid lipofuscinosis Multiple infarct dementia “Binswanger s disease “Small vessel ischemic disease CADASIL Schizophrenia ... Hypothesis Carmela R Abraham 262 viii Contents Other Mechanisms of Neurodegeneration Marina Boziki, Vassilis Papaliagkas, and Magda Tsolaki 277 10 Rational Therapeutics for Alzheimer s Disease and...

Ngày tải lên: 14/03/2014, 21:20

... levels Standard errors were calculated, and Fischer s test was performed to analyze significance This shows approximately a two-fold increase in RCAN1-1 mRNA in the four Alzheimer s disease versus ... (5¢-GACTGGAGCTTCATTGACTGCGAGA-3¢) and the last 24 bases of exon (5¢-CCGGCACAGGCCGTCCACG AACAC-3¢); primers for amplifying exon consisted of the first 25 bases of exon and the last 25 bases of exon ... band, in brain lysates by western blot analysis (Fig 2A) These bands resolved at approximately 70, 38 and 31 kDa on denaturing polyacrylamide gels RCAN1 in Alzheimer s disease Next, antibodies...

Ngày tải lên: 16/03/2014, 11:20

... Ilka Assmann for her assistance with the i.v injections and Nina Schupp and Matthias Schiller for expert technical assistance This work is part of the doctoral thesis of M.R Author details Department ... analyzed using a one way analysis of variance (ANOVA) with a Newman-Keuls post hoc test Tumor sizes were analyzed using the Mann-Whitney-U test Differences in expression of cellular markers as ... Tregs were increased in LRAST-treated mice (Figure 5C and 5D) The decrease of Tregs appeared to be transient since analysis of splenocytes two months after therapy start showed an increased frequency...

Ngày tải lên: 18/06/2014, 22:20

... compare gait variability in the MPTP and 3NP mouse models of basal ganglia disease to a mouse model of motor neuron disease, we also examined gait in a mouse model of amyotrophic lateral sclerosis ... disturbances are characteristic of Parkinson 's disease, Huntington 's disease, and amyotrophic lateral sclerosis Gait reflects several variables, including balance, proprioception, and coordination ... walked with a regularity index of 100% [46] were used for image analyses The treadmill belt was wiped clean between studies if necessary Statistics Data are presented as means ± SE ANOVA was...

Ngày tải lên: 19/06/2014, 10:20

... number of tests / p − value rank Tissue microarrays Tissue microarrays (TMAs) were also constructed (as described in [28]), to allow easier and simultaneous analysis of multiple cases, and held approximately ... carriership and age as covariates where possible Their associations were analysed using logistic regression Chi square analysis was used to determine association with IHC staining False discovery rate (FDR) ... drugs; SNPs: single nucleotide polymorphisms; SP: senile plaques; TASTY: Tampere autopsy study; TMAs: tissue microarrays Acknowledgements Many thanks to Heini Huhtala and Ilkka Seppälä (for assistance...

Ngày tải lên: 19/06/2014, 22:20

... longitudinal assessment of gene dysregulation at different disease stages This could assist in making diagnoses, tracking the disease course and evaluation of disease altering interventions Blood ... homogeneous disease But during the last decade several studies have differentiated early onset or late onset entities as well as fast declining forms Classification and characterization of these disease ... George Angelidis and Panagiotis Georgoulias Part Biomarkers: Steps Toward Rapid Non-Invasive Tests Chapter 10 Cerebrospinal Fluid Based Diagnosis in Alzheimer s Disease 207 Inga Zerr, Lisa Kaerst,...

Ngày tải lên: 28/06/2014, 05:20

... life status of Alzheimer s disease patients and related factors 4.1.1.1 Socio-demographiccharacteristics of Alzheimer s disease patients Age and gender of Alzheimer s disease patients In our study.Patients ... Cerebrovascular disease, cardiovascular disease and cancer - On neuropsychiatric and behavioral symptoms 90.8% of Alzheimer' s patients had at least one neuropsychiatric and behavioral symptomwhich ... was the first to describe the clinical and pathologic characteristics of disease included neurofibrillary tangles and amyloid plaque Alzheimer' s disease was a neurodegenerative disease, indicated...

Ngày tải lên: 25/07/2014, 11:36

... refereed research Figure analysis of transcriptional response patterns Category Category analysis of transcriptional response patterns Intra-tissue expression patterns of genes showing twofold change ... microarray analysis software to measure fluorescence signals and format data for database deposition RT-PCR analysis The expression of selected genes was also analyzed by quantitative RT-PCR using the ... 2004, Activation (fold of changes) Arrays RT-PCR Repression (fold of changes) APC Somatostatin (b) Activation (fold of changes) Carbonic Tenascin C anhydrase White adipose tissue Arrays RT-PCR...

Ngày tải lên: 09/08/2014, 20:20