secretion in pancreatic duct cells

... mechanism is relevant in insulin-secreting b -cells In order to gain more insight into the physiological role of mir-9 in insulin secretion, we have specifically addressed the link between GSIS and ... protein in insulin-secreting b -cells brought about by mir-9 Sirt1 is down-regulated in insulin-secreting cells in a mir-9-dependent manner Discussion Although we have described the targeting in ... insulin-secreting cells was linked to changes in Sirt1 expression in INS-1E cells These reports suggest that, in addition to its significant role in insulin secretion, Sirt1 is a crucial factor in...

Ngày tải lên: 06/03/2014, 00:21

... domain As in the binding studies with plant lectins, we routinely performed experiments to assess concentration dependence and inhibition of galectin binding by haptenic sugar Profiling of galectin ... consistent increase in staining by 50% in p16INK4a-positive cells, relative to control cells, with mass ⁄ isoelectric point (pI) characteristics compatible with galectin-1 As shown in Fig 5, we ... analysis Applying the antigalectin-1-specific immunoglobulin for cell-surface detection, the difference in protein production translated into increased surface presence in the p16INK4a-positive cells...

Ngày tải lên: 16/03/2014, 10:20

... (nuclei) staining; (B) Mitofluor Red 589 (mitochondria) staining; (C) anti-PHB ⁄ anti-rabbit-FITC staining; (D) merge Arrows indicate staining of PHB D proteins have been shown to be involved in endocytosis ... staining; (B) Mitofluor Red 589 (mitochondria) staining; (C) anti-His (exogenous PHB)-FITC staining; (D) merge The arrows indicate staining of His-tagged PHB in (C) or both PHB and Mitofluor in ... determined the level of UCP2 protein and ATP production in RINm5F cells Ethanol increased the UCP2 protein level by 42% (Fig 2C), in direct proportion with ROS production, whereas ATP production...

Ngày tải lên: 29/03/2014, 08:20

... pretreatment results in an inhibition of inflammatory signaling in cells with CF defects Production of IL-8 and other pro-inflammatory cytokines is also governed by mechanisms regulating in their mRNA ... we investigated the following two hypotheses: 1) To determine if the signaling pathway going through the MAPKs: p38, extracellularregulated protein kinase (ERK), and Jun-N terminal protein kinase ... HTE cells (5.6±1.69 vs 4.54±1.88 respectively), whereas Parthenolide significantly inhibits the TNFα- induced increase in IL-8 secretion (p=0.04) (Fig 6C, inset) In a separate experiment AS cells...

Ngày tải lên: 11/08/2014, 03:20

... VEGF 24 h secretion % induction 24 h secretion % induction 24 h secretion % induction 24 h secretion % induction 24 h secretion % induction 24 h secretion % induction 24 h secretion % induction ... Thrombin Thrombin Thrombin 1U + Lepirudin 10 U Thrombin Thrombin Thrombin 10 U + Lepirudin 1U 70000 6000 PBS PBS Adipose Tissue IL-6 pg/ml 4000 1000 Thrombin Thrombin Thrombin 10 U + Lepirudin 1U ... http://www.journal-inflammation.com/content/6/1/4 5000 Thrombin Thrombin Thrombin 1U 10 U + Lepirudin * 4000 ‡ 3000 2000 1000 PBS Thrombin Thrombin Thrombin 1U 10 U + Lepirudin PBS Thrombin Thrombin Thrombin...

Ngày tải lên: 11/08/2014, 08:22

... expressing hA in their b -cells [13,17] Formation of amylin aggregates in the pancreatic islets may thus play an important role in triggering islet b-cell death and dysfunction in T2DM Since all ... mitogen activating protein (MAP) kinase (p38 kinase) followed by caspase-3 activation [24] Mitogen-activated protein kinases (MAPKs) are a group of protein serine ⁄ threonine kinases that play ... of both shifted bands in hA-treated cells In addition, the increased CRE binding activity was suppressed by JNK inhibitor I and SB 203580, implying that hA-evoked CRE binding is mediated by both...

Ngày tải lên: 07/03/2014, 12:20

... results indicate that BFA inhibits the secretion of ZPC by inhibiting the conversion of proZPC to 35-kDa ZPC C-terminal sequence of 35-kDa ZPC In order to determine the C-terminal amino acid ... 7A, the addition of monensin caused an increase in the intensity of the 12-kDa band This is consistent with the result shown in Fig 3B in which monensin inhibits the secretion of 35-kDa ZPC but ... pro-opiomelanocortin in rat pituitary cells [42] This might be due to the fact that proteolytic processing of pro-opiomelanocortin occurs in the secretary granule [43] BFA blocks albumin secretion in rat...

Ngày tải lên: 24/03/2014, 03:21

... SUBSTANCE P INDUCES CHEMOKINE PRODUCTION IN MOUSE PANCREATIC ACINAR CELLS IN A CONCENTRATION-DEPENDENT MANNER 33 2.3.2 SUBSTANCE P OR CAERULEIN INDUCES NFκB ACTIVATION IN MOUSE PANCREATIC ACINAR CELLS ... PANCREATIC ACINAR CELLS 157 6.3.2 INVOLVEMENT OF SFK IN SUBSTANCE P-INDUCED MAP KINASES IN PANCREATIC ACINAR CELLS 157 6.3.3 SUBSTANCE P- INDUCED SFK IS INVOLVED IN ... Gadina et al 2002) All seven STAT proteins share a highly homologous domain structure These include an N-terminal domain which is involved in protein-protein interactions, a DNA-binding domain...

Ngày tải lên: 11/09/2015, 16:06

... channel and the GDLs as in well as the channel inlet conditions ( SR in , RH in , Ptot , y in ) which give Qiin and yiin ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2010 International Energy ... focus on finding a pertinent location allowing to interpret the appearance of liquid water in terms of an ideal operating point 3.1.2 Onset of two-phase regime in the electrodes As mentioned in section ... mixture • The cathode CL is integrated in the membrane to model water production while the anode CL is assumed to be infinitely thin as explained in [21] • Gas crossover in the membrane is neglected...

Ngày tải lên: 05/09/2013, 14:58

... avail- Protein Name Cyclin dependant kinase Cell cycle regulating D-cyclin Cyclin E2 Proliferation of germ cells (POG) Cyclin D-dependant kinase inhibitors p27 Kip1 a Cdk inhibitor Cdk2 CyclinD2 Gcd/Pog ... partner, cyclin D, are responsible for the initial phosphorylation of the Rb protein by regulating cyclin E/Cdk2 and lifting the S-phase inhibition by preventing binding of its inhibitor, p27 ... RNA-recognizing motif involved in splicing, transport, translation and stability of mRNA Knockout results in decrease in the survival of germ cells at the genital ridge RNA-binding protein reported...

Ngày tải lên: 25/10/2013, 21:20

... cells in a culture medium containing [13C3]pyruvate, under normoxia and hypoxia, for h Fig Effect of pyruvate on glutathione content during hypoxia ⁄ reoxygenation in HepG2 cells Cells were incubated ... cancer cells under such stressful conditions [36] Our present work provides new insights into the role of pyruvate in tumor cells during hypoxia We show here that tumor HepG2 cells are inclined ... shown) This is in line with our previous work [29] indicating enhancement of glycolysis activity in the presence of pyruvate Increased glycolysis might thus maintain the ATP supply during oxygen...

Ngày tải lên: 18/02/2014, 16:20

... and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the cells with either 10 ngÆmL)1 toxin B ... levels in response to anisomycin, suggesting that an intact actin cytoskeleton is also necessary for anisomycin signaling Surprisingly, treatment of the cells with latrunculin B alone induced an increase ... untreated cells indicating that a larger pool of total actin exists as filamentous actin in the stimulated cells However, the G- to F-actin ratio seemed to significantly increase as the G-actin levels increase...

Ngày tải lên: 19/02/2014, 16:20

... enrichment in iNOS activity within the particulate fraction of epithelial cells Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... localization of iNOS to apical domains of intestinal epithelial cells Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... the C-terminus of iNOS promotes in vitro interactions with the PDZ protein EBP50 [13] Interestingly, EBP50 has different binding partners including ezrin that can be anchored to the actin cytoskeleton...

Ngày tải lên: 19/02/2014, 16:20

... specifically in VSMC in synovial tissues with active RA In inactive RA sections, sPLA2-IIF showed sporadic and weak staining in individual cells (Fig 4Ca,b), and a few interstitial cells provided intense ... sPLA2-IIA in RA tissues are largely in agreement with a previous study [46] Staining of PLA2-IID was weak in a section of inactive RA tissues, in which scattered staining was located in the subintimal ... showed that, in RA tissues, sPLA2-IIA is distributed in various cells, such as synovial lining and sublining cells and vascular cells, as well as in extracellular matrix fibers [46] In our study,...

Ngày tải lên: 19/02/2014, 16:20

... [s)1] 10)6 ã kass [M)1ặs)1] Cathepsin F Cathepsin K Cathepsin V Cathepsin S Cathepsin H Cathepsin C Cathepsin X Papain Cathepsin La Legumainb Cathepsin Ba Papaina 0.17 0.05 0.35 0.15 0.30 0.15 ... recombinant human cystatin C with the cysteine proteinases papain and actinidin Biochem J 281, 4955 26 Saitoh E & Isemura S (1994) Production of human salivary type cysteine proteinase inhibitors ... that cystatin F was in complex with another M6P containing protein Nevertheless, despite secretion of cystatin F from U937 cells, a high proportion seems to reside intracellularly in lysosomes...

Ngày tải lên: 19/02/2014, 17:20

... retained enzymatic activity in these human T-cell lines Increasing sensitivity to nucleoside analogs in Dm-dNK expressing cells We determined the sensitivity of the untransduced H9 and CEM cells ... expressing H9 cells, as found in the same cells grown in dialyzed medium without HU Effects on mtDNA In the light of the changed dNTP pools in Dm-dNK expressing H9 cells, we wanted to investigate ... Altered dNTP pools in T-cell lines expressing Dm-dNK DNA, RNA and protein synthesis in the Dm-dNK gene transduced cells The extent of DNA, RNA and protein synthesis in Dm-dNK expressing cells was compared...

Ngày tải lên: 20/02/2014, 01:20

... suggested function of cystatin F is hypothetical Although most cystatins inhibiting cathepsin L also inhibit cathepsin S, an inhibitory activity of cystatin F against cathepsin S has not yet been reported ... cystatin F in U937 cells The high portion of intracellular cystatin F led us to fractionate cells to determine the localization of the inhibitor Initially cells were fractionated in a debris ... strongly indicates that the major portion of intracellular cystatin F is present in smaller vesicles In mouse fibroblasts overproducing human cystatin C, intracellular cystatin C routed for direct secretion...

Ngày tải lên: 21/02/2014, 01:21

... age The bFGF binding requires speci®c motifs on highly sulfated HS chains Sodium chlorate, in inducing structural alteration of HS chains, prevents the bFGF binding Our results indicate that ... sulfation on proteins and carbohydrate residues in intact cells without inhibiting cell growth or protein synthesis [35±37], and proteoglycan sulfation in cultured Sertoli cells from 20-day-old ... aromatase mRNA in immature cultured rat Sertoli cells Sertoli cells were incubated for 24 h in the absence of 100 ngáml)1 FSH (lane 1) or in the presence of 100 ngáml)1 FSH and increasing concentrations...

Ngày tải lên: 21/02/2014, 03:20

... naive T cells [40] Dendritic cells are involved in the polarization of the immune response towards a Th1 (large production of interferon-c) or a Th2 (sustained production of interleukins-4 and ... in both human immature dendritic cells and in rat Ó FEBS 2002 The endocannabinoid system in human dendritic cells (Eur J Biochem 269) 3775 Fig FAAH, CB1 and CB2 mRNA expression in dendritic cells ... LPS, interleukin-12 and interferon-c, and upregulated by interleukins-4 and -10 [26,13] Taken together, these data show the presence of a complete endocannabinoid system in human dendritic cells...

Ngày tải lên: 22/02/2014, 07:20