second virial coefficient as an indicator of crystallization
Phase separations in protein solutions a monte carlo simulation study
... the full phase diagram can be calculated by means of perturbation theory or Monte Carlo simulation, which will be discussed in Chapter 2.2 Second Virial Coefficient as an Indicator of Crystallization ... their second virial coefficients It was found that the corresponding phase behaviors of the square-well and Yukawa potentials can be approximated well by that of the AHS model if the range of potential ... Amino Acid and Protein Solutions' To be published by RESEARCH SIGNPOST/ TRANSWORLD RESEARCH NETWORK Jianguo Li, Raj Rajagopalan, and Jianwen Jiang 2008 Polymer-Induced Phase Separation and Crystallization...
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Báo cáo y học: "Coverage and error models of protein-protein interaction data by directed graph analysis" pptx
... give an accurate enumeration of the sets of tested baits and tested prey in an experiment, and often the published data not contain sufficient detail to allow identification of these sets As a ... sequence, and can serve as a proxy for protein abundance [38] To visualize the association between such proteins and CAI, we plotted diagrams of the adjacency matrix If the value of CAI is associated ... observed, and they can be used to understand better the sources of error Measurement error can be divided into two categories: systematic and stochastic We have shown that there are proteins with an...
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Báo cáo y học: "The retinoic acid binding protein CRABP2 is increased in murine models of degenerative joint disease" pot
... expression to type X and a decrease in ACAN expression accompanied by an increase in catabolism of collagen type II and ACAN was observed In this regard, retinoic acid has been shown to enhance chondrocyte ... with 4% isoflurane and L/minute oxygen Once mice were anaesthetised they were transferred to a mask and maintained on 2% isoflurane and 0.8 L/minute oxygen The surgical area was shaved and a three-part ... degeneration in cartilage explants, and this has been associated with increased activity of various aggrecanases Knockout animals of the gene encoding the aggrecanase ADAMTS5 are protected to...
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Báo cáo y học: " Characterization of protein tyrosine phosphatase H1 knockout mice in animal models of local and systemic inflammation" docx
... Latency of each paw withdrawal was recorded and mean values of left and right paws were used as reaction index for the individual animal A cut-off of 25 seconds was used to avoid tissue damage in case ... the FERM (band 4.1, ezrin, radixin, moesin) domain of PTPH1 is necessary for the inhibition of Mek, Erk, Jnk and AP-1 and also for localization of the phosphatase on the plasma membrane of Jurkat ... IL-10 has not an exclusive proinflammatory action [56] and it has been demonstrated in an LPS-model that IL-10 release increases as MCP-1, IL6 and TNFα [57] Thus, our results on overall increased...
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Báo cáo y học: " Differential expression and function of breast regression protein 39 (BRP-39) in murine models of subacute cigarette smoke exposure and allergic airway inflammation" pps
... support of Joanna Kasinska and Sussan Kianpour, the secretarial assistance of Marie Colbert, and Gordon Gaschler for discussion and preparation of the manuscript Additional technical assistance was ... BAL of BRP-39 and WT mice Flow cytometric analysis of lung preparations further revealed no difference in numbers of Th2 cells (as assessed by T1/ST2 and IL-5 signal) and DC activation (as assessed ... redundant in the adjuvant properties of cigarette smoke This implies a different mechanism of sensitization when cigarette smoke is utilized as an adjuvant This is not an unprecedented assertion as...
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FOAMING PROPERTIES OF PROTEINS.pdf
... (FS) was observed around a gas flow rate (FR) of 20 ml/min and a pH of 5.0 At least three response surface graphs for each parameter [(percent of gas initially in the foam (Gi), volume of liquid ... related to the increased viscosity of the liquid phase and a decreased rate of protein denaturation (Buckingham, 1970; Mita et al., 1977) The rate of drainage of liquid from many foams obeyed the ... significantly increased as the pH approached the IEP (Musselwhite and Litchener, 1967) The surface viscosity and elastic potential of the protein film also increase in the isoelectric zone (Cumper and...
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Models of motherhood in the abortion debate - self-sacrifice versus self-defence
... symbiotic ideal of mother and child that cultures so often idealize However, given the quality and quantity of the transformations of a woman’s body and liberty resulting from a fetus, if a woman does ... Models of motherhood in the abortion debate Roles and goals As we know from the work of policy analysts, such as Joyce Gelb and Marian Palley (1987), the problem of deWnition has been at the core of ... precedents mandate that the state must provide a pregnant woman with assistance to protect her from that harm, that is, with assistance to procure an abortion as the necessary means for stopping...
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Tài liệu Báo cáo khoa học: Animal models of amyloid-b-related pathologies in Alzheimer’s disease docx
... majority of amyloid plaques Animal models of Alzheimer’s disease Table Metabolic levels of amyloid-b (Ab) in brain, cerebrospinal fluid (CSF) and plasma of young transgenic mice and healthy human Measures ... 1391 Animal models of Alzheimer’s disease O Philipson et al Transgenic animal models Models devoid of any disease-causing APP mutations Animal models expressing wild-type (wt) human APP are of ... with an autosomal dominant mode of inheritance, account for < 2% of all AD cases Onset is most often before 65 years of age, and the penetrance is nearly always complete The purification and partial...
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Tài liệu Báo cáo khoa học: Bacitracin is not a specific inhibitor of protein disulfide isomerase pptx
... many in vitro PDI assays, the assay is indirect, with complex kinetics For this reason, we decided to measure the lag-phase of the reaction, i.e the time before an apparent increase in absorbance ... absorbance of 0.1 was recorded The addition of PDI to the assay accelerated the reduction and precipitation of the B-chain significantly, decreasing the lag-phase (Fig 2A) When bacitracin was included ... reduction of the B-chain of insulin causes precipitation that can be followed as an absorbance increase at 540 nm (A) Representative changes in absorbance as a function of time From left to right,...
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Tài liệu Macro-Finance Models of Interest Rates and the Economy pdf
... December 12, 2007, the Bank of Canada, the Bank of England, the European Central Bank (ECB), the Federal Reserve, and the Swiss National Bank jointly announced a set of measures designed to address ... that the recent financial and economic crisis—the Great Panic and Recession of 2008 and 2009—will both rearrange the economic landscape and affect the focus of economic and financial research going ... Macro-Finance Models of Interest Rates and the Economy Glenn D Rudebusch∗ Federal Reserve Bank of San Francisco Abstract During the past decade, much new research has combined elements of finance,...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
... same sites of yeast vector pNS61 was cut with Spe1 and Xho1 and cloned into same sites of yeast vector of 2% glucose or raffinose and were harvested during log phase and treated with zymolyase to ... formaldehyde, was measured as previously described [56] In all cases, both enzyme and zerotime blanks were also analyzed NDMA-d activity was assayed by the Anderson & Angel method [57] as modified ... TOM70), which enables the translocation of proteins through the outer membrane and the inner membrane channel-forming proteins, TOM40 and translocase of inner membrane 23 (TIM23) [18–20] Our studies...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... entire mixture of highly charged c¢- and z¢Æ-type fragment ions, and then reacting them with a second anion that functions as a base rather than an electron donor The carboxylate anion of benzoic ... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of ... peptide is thus 6073 Da The measured mass is 363 mass units higher than predicted Further analysis of c¢-type ions (singly and doubly ETD-MS analysis of peptides and proteins charged) indicates...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... principle, an automated HPLC apparatus equipped with an autosampler, and can be purchased from a variety of companies; and, at least in our hands, HPLC solvent gradients and flow rates can nowadays ... Puype M, Van Damme J & Van Montagu M (1985) Protein-blotting on Polybrene-coated glass-fiber sheets A basis for acid hydro- 6286 12 15 lysis and gas-phase sequencing of picomole quantities of protein ... LJ, Tan TW & Ranganathan S (2006) SVM-based prediction of caspase substrate cleavage sites BMC Bioinformatics (Suppl 5), S14 Kuhn K, Thompson A, Prinz T, Muller J, Baumann C, Schmidt G, Neumann...
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Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt
... the basal and elevated amounts of procaspase-8 in both control and IFN-treated cells and there was an approximately two-fold increase in the amount of the p18 large subunit of active caspase-8 ... that of downstream effector caspases also, is enhanced in cells treated with the combination of IFNa and TRAIL, relative to TRAIL alone In view of the cleavage of caspase substrates such as BID and ... downregulation of translation seen in the presence of IFN correlates with increased caspase activity Although the inhibition of protein synthesis requires caspase activity, it precedes the appearance of an...
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Tài liệu Báo cáo khoa học: "Parsing with generative models of predicate-argument structure" pptx
... ÆÈ opened , and Æ doors Dutch has a much freer word order than English The analyses given in Steedman (2000) assume that this can be accounted for by an extended use of composition As indicated ... shares and bought In contrast to the SD model, it can use all instances of shares as the subject of a passive verb in the training data to estimate this probability Therefore, even if shares and ... Journal task, the overall performance of this model is lower than that of the SD model of Hockenmaier and Steedman (2002b) In that model, words are generated at the maximal projection of constituents;...
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Tài liệu Master Thesis Health Sciences: Expert Elicitation to Populate Early Health Economic Models of Medical Diagnostic Devices in Development pdf
... spicular Mass shape The shape of a mass can also be an indicator of malignancy Different appearances of masses are for example: round/oval, or lobular Also the shapes within a mass can be important ... profile plots can contribute in the assessment of breast lesions During the assessment of different tumor characteristics it was observed that the mass margins and mass shape are important aspects ... detection of breast cancer in two settings First, as a screening test for women at high risk of developing breast cancer, for instance those with mutations of BRCA1 and BRCA2 genes Secondly, as an adjunct...
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Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
... accuracy of the prediction of the x class with respect to the overall amount of total predictions made for that class The prediction efficiency has a P(x) value of 0.72 and this is by far higher than ... values are increasing at increasing reliability index (R) The rate of false positives can be evaluated as [1-P(c,R)] and is decreasing at increasing R values When R ‡ 7, [1-P(c)] decreases from 0.16 ... obtained with a random predictor (in this case the Q2 and C-values are equal to 0.60 and 0, respectively) Another scoring index for the contact (c) class is the probability of correct predictions...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... characteristics of L6 cells were analysed and then the level of recombinant hUCP3 expressed was determined by Western blotting (Fig 3A, upper and medium panels) Quantification was based on a standard curve ... wavelength was 470 nm and emission was measured at 600 nm Measurement of membrane potential DY The membrane potential was recorded using the fluorescent probe 3,3¢-dihexyloxacarbocyanine iodide ... membrane potential of the permeabilized cells resuspended in buffer A at pH 7.4 ( · 106cellsÆmL)1) was measured at room temperature using an excitation and an emission wavelength pair of 485 and...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... solid of purity exceeding 99% as assessed by HPLC analysis of the nal materials Identity and purity of peptide was conrmed by mass spectrometry, utilizing a Finnigan MAT TSQ 700 (Finnigan MAT, San ... Germany) Acetic anhyă dride (Ac2O) was obtained from POCh (POCh S.A., Gliwice, Poland) Triisopropylsilane (TIS) was obtained from Lancaster (Lancaster Synthesis GmbH, Muhlheim ă am Main, Germany) ... Logarithmic values of *K and cK constants for model peptides and other XYH peptide analogues, representing the high-end and the low-end of anity series The values of constants were calculated...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... membrane The membrane was treated with a rabbit anti-iNOS polyclonal antibody or rabbit anti-PKC polyclonal antibody at : 1000 dilution and anti-(rabbit IgG) Ig horseradish peroxidase conjugated antibody ... of antioxidants such as butylhydroxyl anisol (BHA) and ascorbic acid (AsA) on the enhancing effect of TS on LPS/IFN-induced NO production Unexpectedly, neither BHA nor AsA affected the TS-enhanced ... moiety and the hydrophobic isoprene moiety The enhancing effect of TS on LPS/IFN-induced NO production was inhibited by the coexistence of a-T, TA and TN (Figs and 4), but not of the antioxidants...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25