screening for antibodies against mica by luminex flow cytometry

... either a perfect shield of site B afforded by bilirubin, or, otherwise, by an alternative conformation of the bilirubin– bilitranslocase complex, totally missed by antibody B Cyanidin 3-glucoside ... uptake, inhibited by antibodies against bilitranslocase, in microsomal, plasma membrane and tonoplast vesicle fractions In addition we showed that a protein cross-reacted with these antibodies in ... depends on the proton electrochemical potential generated both by the H+-ATPase and H+-PPiase [11] By analogy, this model may also include the protein encoded by the tt12 gene in Arabidopsis...

Ngày tải lên: 20/02/2014, 01:20

... forward primer to allow subsequent in vitro transcription After denaturing at 98°C for 30 sec, PCR reactions were cycled 30 times at 98°C for sec, 68°C for 20 sec, 72°C for followed by 72°C for ... sub-fragments amplified for genomic analysis Chromosomal location and genomic mapping of the BRCA1 locus and sub-fragments amplified for genomic analysis The correct size for each amplicon is shown ... transcription using T7 Megascript kit (Ambion) The reaction was run for hours at 30°C Isolation of amplified RNA (aRNA) was performed by TRIZOL purification Three micrograms of purified aRNA were fluorescently-labeled...

Ngày tải lên: 18/06/2014, 15:20

... Immunoresearch, West Grove, PA) for secondary binding The antibodies for the other markers were all commercial monoclonal antibodies which were specific for porcine antigens or were anti-human or anti-mouse ... Representative input and output for the “Expander” program analytical software such as FlowJo (http://www.flowjo com) and FCS Express (http://www.denovosoftware com) The gating strategy for this study is illustrated ... control Next, we looked for sets that were interesting based on coefficient of variation (CV, standard deviation divided by mean) We computed Average CV by calculating CVs for each donor across...

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 07/08/2014, 18:21

... identify mAbs for research in rabbits As part of our continued effort to develop mAbs critical to our research efforts in ruminants, we have developed a flow cytometric approach for initial identification ... Analysis of monoclonal antibodies specific for the γδ TcR Vet Immunol Immunopathol 1996, 52, 275-283 11 Davis WC, Davis JE, Hamilton MJ Use of monoclonal antibodies and flow cytometry to cluster ... rabbits varied from six months to about two years Preparation of leukocytes for flow cytometry Because of the tendency for T lymphocytes to bind to erythrocytes, separation medium could not be...

Ngày tải lên: 07/08/2014, 20:23

... pre-denaturation at 98°C for min; 32 cycles of denaturation at 98°C for 20 sec, annealing at 56°C for 20 sec, and extension at 72°C for 80 sec; followed by a final extension at 72°C for 2.2 Construction ... incubated for h at 37°C at 200 rpm before harvesting the cells by centrifugahttp://www.medsci.org Int J Med Sci 2011, tion (15,000×g, 20 min, 4°C) and the cell pellets were washed and lysed by sonication ... days before harvesting the spleen cells Experiments with injected mice were performed under the guidelines for care and use of experimental animals 2.5 Cellular fusions When the anti-NPM-mA antibodies...

Ngày tải lên: 08/08/2014, 18:21

... anti-PM/Scl-75 antibodies, especially when the major isoform PM/Scl-75c is used as an antigen source [9,10] The percentage of patients presenting antiPM/Scl-75c antibodies is supposed to exceed that for ... a popular technique for the simultaneous detection of several autoantibodies As recently shown and exemplified for the determination of anti-topoisomerase I (anti-topo I) antibodies, LIA provides ... from each patient Antibody detection by line immunoblot assay For the detection of the different anti-PM/Scl antibodies, a profile LIA was developed and provided by EUROIMMUN AG (Lübeck, Germany)...

Ngày tải lên: 09/08/2014, 01:22

... Positive (>90st rank for Kit B and >92nd rank for Kit A) 0.032 0.767 0.247 0.049 Negative (92nd rank for Kit A) -0.003 ... alphaherpesvirus in reindeer by the ranks of the results Comparison Comparison of three serological kits for detecting antibodies against alphaherpesvirus in reindeer by the ranks of the results ... test was needed Three commercial ELISA kits for detecting antibodies against BoHV-1 in cattle were evaluated regarding their ability to detect antibodies against alphaherpesviruses in reindeer: one...

Ngày tải lên: 12/08/2014, 18:22

... collected at the herd visit for analysis of serum antibodies to L intracellularis and for L intracellularis DNA by nested PCR, respectively [16] Sera were analysed by the blocking ELISA (Product ... responsible for the coordination of the work, for collecting the samples, for the PCR analyses and drafted the manuscript PW were responsible for the serological analyses UE were responsible for the ... monoclonal antibodies for capture and blocking to ensure a defined analytic sensitivity and specificity and, assumingly, an increased diagnostic specificity However, if antibodies are formed towards...

Ngày tải lên: 12/08/2014, 18:22

... pressure at the receiver for the duration of each determination 5.8 Temperature Measuring Device (Liquid-in-glass thermometer)—Conforming to specifications for ASTM Thermometer 114C for air baths or ASTM ... inspect the filter assembly before each test Filters obtained from the manufacturer are already standardized Appendix X1 provides a procedure for checking the filter performance, if desired 8.2.1 ... new filter Alternately, return the filter to the manufacturer for verification; Appendix X1 provides a procedure for checking the filter performance 8.3 Pour 200 mL of clean, dry fuel into each of the...

Ngày tải lên: 20/10/2014, 17:18

... Year Programme for Children LOOK, LISTEN AND LEARNl Sets 1-4 An Integrated Course for Children New Concept English Uniform with this Volume: FIRST THINGS FIRST: An Integrated Course for Beginners ... motorists the world over All the discomforts associated with it have been miraculously whisked away For a modest outlay, you can have a comfortable, insulated tent For a not-so-modest outlay, you can ... pummelled and massaged by weird machines The really wealthy diet-mongers pay vast sums for 'health cures' For two weeks they can enter a 'nature clinic' and be starved to death for a hundred guineas...

Ngày tải lên: 19/09/2016, 17:00

... which in their entirety can be responsible for the constricted sensitivity against alkylating agents It is clear that effort is necessary to search for new forms of treatment modalities like drug ... Constitutional formula of the investigated TMZ-BioShuttle Cell Cycle and Cell Death Analysis The effects on the cell viability and the cell cycle distribution were determined by DNA flow cytometry Flow ... (DAPI) for staining the cell suspension was performed Each histogram represents 30.000 cells for measuring DNA-index and cell cycle For histogram analysis we used the Multicycle program (Phoenix Flow...

Ngày tải lên: 26/10/2012, 09:48

... upland vegetable fields, forests and livestock farms—and the TN concentration in the drainage water varies across the points Therefore, we believe that the area is suitable for comparing the effect ... and collect runoff data for the period from August 2001 to July 2002 Sampling point A is the mouth of the river; point B, the drainage in the paddy fields surrounded by forests; point C, the drainage ... differ depending on the land use, the dissolution rate (α) is set to 0.5 for the upland fields, 0.0 for livestock and 1.0 for others, that is, the nitrogen load from fertilizers is divided into...

Ngày tải lên: 05/09/2013, 09:08

... expects certain form information Building an HTML Page with a Form Forms are very useful when you want to get information from the user To illustrate how this is done, look at the code for the whatsName.html ... "mystery" value = "secret formula"> All the elements that will allow user interaction are placed inside a pair The most common form element is the ... enters information into a plain HTML form and hits the submit button The PHP program doesn't execute until after the user has submitted a form The program takes the information from the form and...

Ngày tải lên: 17/10/2013, 10:15

... Miss Nguyen Quynh Hoa from Foreign Language Department for her help and encouragement My sincere thank also goes to Ms Tran Ngoc Lien, Dean of Foreign Language Department for her permission to take ... University Therefore, it was a motivation for the author of the reasons to discuss Hopefully, this study will help students grow awareness of problems that they might have not noticed before and raise ... 2.2.2.1 Definition According to the Oxford Advanced Learner’s Encyclopedic (1992:192), consonants are “speech sounds made by completely or partly stopping the flow of air breathed out through the...

Ngày tải lên: 17/12/2013, 20:37

... for Joint Products and By- Products ACCOUNTING FOR BY- PRODUCTS AND SCRAP Because the distinction between by- products and scrap is one of degree, these categories have been discussed together by ... furniture Therefore, depending on the company, sawdust and chips may be considered a joint product or a by- product Sometimes a byproduct will be accidentally discovered by good fortune An interesting ... the by- products/scrap and the need for additional processing after split-off As the sales value of the by- product/scrap increases, so does the need for inventory recognition Sales value of the by- products/scrap...

Ngày tải lên: 18/12/2013, 09:12

... 25 • Edit for truth and accuracy • Edit for organization • Edit for paragraph structure • Edit for sentence structure • Edit for word choice • Edit for spelling and punctuation • Edit for conciseness ... of foreign languages for their supports in providing with constructive suggestions for this thesis I would like to thank the students at Nguyen Xuan On high school for their impartial help by ... more detail a Memory strategies: “aid in entering information into long – term memory and retrieving information when needed for communication” (Oxford, 1990, cited in Clouston 1997) i.e Memory strategies...

Ngày tải lên: 20/12/2013, 18:33

... control overflow (critical depth) hc gradually varied flow rapid varied flow gradually varied flow rapid varied flow Fig 4.1 Examples of non-uniform flow 4.1.2 Accelerated and Retarded flow An idealized ... varied flow rapid varied flow gradually varied flow rapid varied flow upstream control critical depth hc supercritical flow control hydraulic jump rapid varied flow subcritical downstream flow ... allows the flow to be treated as one-dimensional with no transverse pressure gradients other than those due to gravity For subcritical flows the flow situation is controlled by the downstream flow...

Ngày tải lên: 20/12/2013, 19:15

... http://thomsonlifestylecentre.com/pre-marital-health -screening Premarital screening- http://thomsonlifestylecentre.com/pre-marital-health -screening Educated customers have forced food companies to supply low-body ... just accept By looking into making simple changes for your child's daily routine, its likely to safeguard their heart, renal system, eyes and braches in the damage frequently triggered by diabetes, ... Premarital screening- http://thomsonlifestylecentre.com/pre-marital-health -screening Based on the Cdc and Prevention in Atlanta, U s States,...

Ngày tải lên: 16/01/2014, 14:27

... to recommend for or against routine CBE alone to screen for breast cancer The USPSTF also concluded that the evidence was insufficient to recommend for or against teaching or performing routine ... 0.75-0.99) for breast Breast Cancer Screening 13 Oregon Evidence-based Practice Center cancer mortality for women randomly assigned to mammography screening The number needed to invite for screening ... access to screening, or existence of co-morbidities, for example, could inform screening practice Studies of older women are essential in order to determine appropriate screening regimens for them...

Ngày tải lên: 14/02/2014, 21:20