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... 73, 611 – 620 12 Schlessinger, J (19 93) How receptor tyrosine kinases activate Ras Trends Biochem Sci 18 , 27 3 27 5 13 Pawson, T & Scott, J.D (19 97) Signalling through scaffold, anchoring, and adaptor ... J.M (19 95) Negative feedback regulation and desensitization of insulin- and epidermal growth factor-stimulated p21ras activation J Biol Chem 27 0, 25 320 25 323 42 Huang, C.Y & Ferrell, J.E Jr (19 96) ... Opin Cell Biol 12 , 18 6 1 92 44 Ron, D & Kazanietz, M.G (19 99) New insights into the regulation of protein kinase C and novel phorbol ester receptors FASEB J 13 , 16 58 16 76 Ó FEBS 20 04 Synergistic...

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... experiments (sequence 11 4 22 5) Two peptides belonging both to the 11 4 22 5 sequence and exposed regions of subdomain were tested (1 12 – 12 5 and 11 9 – 1 32 peptides) Interaction of the 15 9 19 3 fragment with ... fragment was labelled with FITC and mixed with 1 12 12 5 and 11 9– 1 32 actin peptides As shown in Fig 8B, the 11 9 1 32 peptide does not perturb FITC In contrast the 1 12 12 5 peptide induces a fluorescence ... sequences Peptide 15 9 19 3 Kd ELISA Peptide 15 9 19 3 Kd fluorescence Cofilin Kd Reference for cofilin Actin G Actin F 1 10 18 28 84 10 3 1 12 12 5 11 9 1 32 347–365 338–348 360–3 72 355–375 1. 3 mM ND No binding...

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... ns-LTPs 20 000 15 000 10 000 5000 -Molar ellipticity (deg·cm2·dmol 1) B 20 000 C 20 000 15 000 10 000 5000 15 000 10 000 5000 10 [dithiothreitol] (mM) 12 Fig CD signal recorded at 22 2 nm and 10 °C ... (mdeg) 20 000 10 000 15 000 20 000 –30 000 19 0 20 0 22 0 24 0 20 000 10 25 0 20 Wavelength (nm) C 40 000 D CD (mdeg) 20 000 CD (mdeg) 40 60 80 90 80 90 Temperature (ºC) –5000 10 000 15 000 20 ... 20 000 –30 000 19 0 20 0 22 0 24 0 25 0 Wavelength (nm) 20 000 10 20 40 60 Temperature (ºC) Fig CD spectra (A, C) and temperature scans recorded at 22 2 nm (B, D) obtained for nPar j 1 Par j in mM...

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... replicon [Con -15 (15 /1, 15 /2, 15 /3), Con -17 (17 /1, 17 /2, 17 /3) and Con -24 (24 /1, 24 /2, 24 /3)] were prepared The nine different replicon cell lines were maintained in the presence of IFN-α2b (10 00 IU/ml) ... cell line (15 -1, 15 -2 and 15 -3) are comparable to sensitive cells (Fig 6A) Similar results were obtained with 17 -1, 17 -2 and 17 -3 (Fig 6B) and 24 -1, 24 -2, 24 -3 (Fig 6C) The receptor binding studies ... radioactivity specifically bound to the cell surface at each concentration of interferon was determined http://www.virologyj.com/content/4 /1/ 89 10 11 12 13 14 15 16 17 18 19 20 Competing interests The...

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... 7:354 http://www.virologyj.com/content/7 /1/ 354 18 19 20 21 22 23 24 25 26 27 Page of immunodeficiency virus type integration by using a specific, sensitive and quantitative assay based on real-time ... immunodeficiency virus -1 (HIV -1) J Clin Invest 19 92, 89 :17 6 -18 3 Butler SL, Hansen MS, Bushman FD: A quantitative assay for HIV DNA integration in vivo Nat Med 20 01, 7:6 31- 634 doi :10 .11 86 /17 43- 422 X-7-354 Cite ... 19 93, 67 :11 69 -11 74 10 Stevenson M, Stanwick TL, Dempsey MP, Lamonica CA: HIV -1 replication is controlled at the level of T cell activation and proviral integration Embo J 19 90, 9 :15 51- 1560 11 ...

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... Acute cortical damage to auditory processing structures might be assessed objectively in a complementary manner, via studies of the MMN [10 3] 1 52 10 11 12 13 14 15 16 17 18 Closing Remarks Each ... Revista de Neurologica 20 02; 34 :10 17 -1 020 10 3 Näätänen R, Escera C Mismatch negativity: clinical and other applications Audiology and Neurootology 20 00; 5 :10 5 -11 0 Figures Figure Archetypical waveform ... of interest exists 19 20 21 22 23 24 References Sabatini RME Mapping the brain Brain and Mind Magazine 19 97; Pritchard WS Psychophysiology of P300 Psychological Bulletin 19 81; 89:506-540 Pritchard...

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... Dual-Homed Devices NPE 11 NPE10 ge2/4.4 gi3/0/0/0 primary NPE 12 fa1/0.4 ge2/4 .2 backp gi3/0/0 /11 ge1/3.4 72a fa1/0 .2 NPE14 ge1/3 .2 Note 19 1978 Figure 4 -2 The configurations on the primary and backup interfaces ... fa1/0.4 description 72a to npe 12 – bridged encapsulation dot1q ip address 10 0.4 .1. 12 255 .25 5 .25 5.0 interface fa1/0 .2 description 72a to npe 12 - xconnect encapsulation dot1q ip address 10 0 .2. 1. 12 ... ge2/4.4 gi3/0/0/0 primary NPE 12 fa1/0.4 ge2/4 .2 backp gi3/0/0 /11 ge1/3.4 72a fa1/0 .2 NPE14 ge1/3 .2 19 1978 Figure 4-3 This is the configuration at NPE10: interface ge2/4.4 description npe10 to...

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... ˚ 2d2 (A2) R factor Wild-type CeMT -1 KO S S O S 4 3.3 2. 49 2. 53 1. 98 2. 52 0. 026 0. 017 0. 015 0. 013 74.3 48.6 40.5 O O 4 2. 00 2. 00 0. 010 0. 015 39 .1 30.9 CeMT -2 KO S 2. 48 0. 028 78.8 3.3 2. 51 1.98 ... Journal 27 7 (20 10 ) 25 31 25 42 ª 20 10 The Authors Journal compilation ª 20 10 FEBS 25 33 C elegans metallothioneins discriminate between metals S Zeitoun-Ghandour et al MT isoform log KZn 12 . 0 ± 0 .1 12 . 0 ... observed in spectra of the CeMT -1 knockout mtl -1 (tm1770) and the CeMT -1 A CeMT -2 KO Normalised signal CeMT -1 KO Wildtype 26 700 26 710 26 720 26 700 26 740 26 780 26 820 26 860 Energy (eV) B Double...

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... 12 7 ± 15 735 ± 11 4 11 6 ± 44 6 61 ± 11 7 714 ± 52 13 40 ± 24 1 1960 ± 335 14 6 ± 11 > 5000 13 6 ± 19 > 5000 > 5000 > 5000 > 5000 > 5000 ND ND > 5000 12 8 3 ± > 5000 13 43 ± > 5000 > 5000 ND > 5000 12 9 1 ... GST-hEAG (1 20 6)_F151NL 15 4N GST-hEAG (1 20 6)_F151SÆL154SÆL158S GST-hEAG(674–867) GST-hEAG(674–867)_F 714 SFÆ 717 S GST-hEAG(649–867) GST-hEAG(649–867)_F 714 SÆF 717 S GST-hEAG(649–867)_F 714 SÆF 717 SÆL674NÆI678N ... extension PCR [28 ] EF-hand mutants used were: hCaM-EF 12 (D21AÆD57A), hCaM-EF34 (D94AÆD130A), and hCaM-EF 12 3 4 (D21AÆD57AÆD94AÆD130A) (H)6-hCaM and (H)6-hCaM EF-hand mutants were cloned into pQE30,...

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... 0.88 1. 90 0.94 ± ± ± ± ± ± 4 .29 4 .25 2. 44 5 .20 3 .25 88.6 16 1.0 53.4 48 .2 945.0 ND 1. 00 1. 01 1.76 0.83 1. 32 0.05 0.03 0.08 0.09 0.005 ND 1. 00 0. 51 0.97 ± ± 2. 35 2. 90 2. 25 ± ± 23 .50 56.86 23 .20 ND ... Ala 31 des- (2, 3)-Ala 31 Leu60 Ala31Leu60 IGF-II and analogues IGF-II Des- (1 6) Arg6 Leu27 Des- (1 6 ,10 )-Leu27 kd ´ 10 )3 (1/ s) Kd ´ 10 )9 (M) Relative Kd 2. 70 2. 52 3.60 3.65 2. 89 ± ± ± ± ± ± 1. 16 1. 07 ... 40 F 18 0 13 0 10 0 70 20 10 50 450 850 0 50 450 850 G C 18 0 50 10 0 30 10 20 50 450 850 D 50 450 850 H 18 0 50 10 0 30 10 20 50 450 850 Time (s) 50 450 Response (RU) Response (RU) Ó FEBS 20 02 Fig BIAcore...

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... 69 gstk -1 (zk1 320 .1) B AA position rGSTK1 GSTK -1 GSTK -2 4 62 51 150 1 82 15 1 gstk -2 (d2 024 .7) E Petit et al 380 599 50 469 10 0 15 0 20 0 C that C elegans ZK1 320 .1 and D2 024 .7 genes belong to the kappa ... composition of control and gstk -1, gstk -2 and double gstk -1( RNAi) and gstk -2( RNAi)-treated animals (see also Tables S1–S4) Depletion of both gstk -1 and gstk -2 leads to a decrease in the 18 :1w7 fatty acid, ... ⁄ VV) at the N-terminus of the GSTK -2 sequence For these reasons, ZK1 320 .1 and D2 024 .7 genes were renamed gstk -1 and gstk -2 50 32 14 7 Fig Genomic structure and intron positions in C elegans gst...

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