scavenging of reactive oxygen species
Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in ... protein)1, with unit of SOD activity being defined as the amount of proteins causing half-maximal inhibition of the rate of NBT reduction GST activity was assayed in the supernatant of cell lysates, ... activation of NF-kappa B and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor EMBO J 12, 2005–2015 57 Chen, Q., Olashaw, N & Wu, J (1995) Participation of reactive oxygen species...
- 11
- 412
- 0
báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
Ngày tải lên :
20/06/2014, 00:20
... oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species by neutrophils in contact with PM2.5 A In vitro production of reactive oxygen and nitrogen species by neutrophils ... performed using the GraphPad Prism software (version 5.0 for Windows; GraphPad Software Inc., La Jolla, CA) Quantification of reactive oxygen species To measure the amount of free radicals generated, ... displays reactive oxygen species (ROS) generation as a function of enzyme protection, which is altered in the asthma group Page of 11 (page number not for citation purposes) Journal of Occupational...
- 11
- 511
- 0
báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx
Ngày tải lên :
12/08/2014, 03:20
... Patterns of hydrogen peroxide scavenging mechanisms In order to investigate the efficiency of hydrogen peroxide scavenging mechanisms, global H2O2 -scavenging enzyme activities and transcript levels of ... component of the reactive oxygen gene network of Arabidopsis [67] Involvement of sAPX in response to oxidative stress has also been reported by transcriptional induction in the presence of H2O2, ... inhibition of electron transfer through PSI, was associated with a general repression of transcripts encoding the different isoenzymes of SOD and with a decrease of the global activity of this O2. scavenging...
- 18
- 265
- 0
Báo cáo y học: " Reduced levels of reactive oxygen species correlate with inhibition of apoptosis, rise in thioredoxin expression and increased bovine leukemia virus proviral loads" potx
Ngày tải lên :
12/08/2014, 23:22
... Control % of 8-OHdG positive B cells Red CM-XROS 60 Profile of fluorescence intensities 50 40 30 20 10 Control Figure of Analysis DNA oxidative damage Analysis of DNA oxidative damage After 24 h of ... in the absence and the presence of mM VPA (Sigma/Aldrich) and the percentages of apoptotic B cells were determined as described above Analysis of reactive oxygen species (ROS) production Intracellular ... production of reactive oxygen species Cancer Res 2006, 66:6563-6569 Willems L, Kettmann R, Dequiedt F, Portetelle D, Voneche V, Cornil I, Kerkhofs P, Burny A, Mammerickx M: In vivo infection of sheep...
- 10
- 328
- 0
Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
Ngày tải lên :
05/03/2014, 23:20
... cellular ROS and reactive nitrogen species (RNS) production The increased production of reactive species was apparent after h of stimulation Since UCP2 is described as a regulator of ROS production, ... protein Measurement of oxygen consumption Measurements of oxygen consumption were performed using an oxygen electrode (Clark electrode; Rank Brothers Ltd, Cambridge, UK) Oxygen consumption rates ... protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria FEBS Lett 416, 15–18 Ulevitch RJ & Tobias PS (1999) Recognition of gramnegative bacteria and endotoxin...
- 11
- 430
- 0
Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
Ngày tải lên :
16/03/2014, 06:20
... Structure, regulation and evolution of Nox H Sumimoto Introduction Reactive oxygen species (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism On the other ... with the 3-phosphate moiety of the bound PtdIns(3)P, NH2 and NE in the side chain of this residue forming hydrogen bonds with oxygens of the 3-phosphate; NH1 and NH2 of Arg105 form hydrogen bonds ... appearance of Nox1 and the Nox1 organizer Noxo1 in this subphylum of the Chordata The coincidence of the emergence of Nox1 and its regulatory proteins may be related to two rounds of whole genome...
- 29
- 618
- 0
Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc
Ngày tải lên :
23/03/2014, 09:21
... upregulated upon 48 h of hypoxia, and that this increase is dependent on the tissue and the severity of hypoxia The mechanism of induction of Cygb is regulated by HIF-1 The aim of this study was ... tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we tried to shed light on the ROS -scavenging function of Ngb and on the mechanism of cell death ... (48 h) and after reoxygenation (48R8, 48R24: 48 h of hypoxia, followed by h or 24 h of reoxygenation) Positive control: U937 monocytes treated with 50 lM etoposide for h such as reactive thiols,...
- 6
- 391
- 0
Báo cáo Y học: Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species pot
Ngày tải lên :
31/03/2014, 21:21
... rupture and preparation of membrane vesicles of V alginolyticus cells were performed under exclusion of dioxygen In addition, the effect of enzymes that detoxify reactive oxygen species (superoxide ... N2 In order to investigate the influence of dioxygen and NADH on the activity of the Na+-NQR, membrane vesicles were prepared (A) in the presence of dioxygen without additions (B) with 300 UÆmL)1 ... and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen Characterization of redox cofactors in membranes from...
- 6
- 307
- 0
Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps
Ngày tải lên :
09/08/2014, 08:23
... role of reactive oxygen species in homeostasis and degradation of cartilage Osteoarthritis Cartilage 2003, 11:747-755 Panasyuk A, Frati E, Ribault D, Mitrovic D: Effect of reactive oxygen species ... the nomenclature of free radicals, the term 'reactive oxygen species' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen species and other oxidant species The relationship ... level of VEGF is the activation of AP-1 (activator protein 1), with subsequent activation of the TPA (12-O-tetradecanoylphorbol-13-acetate) element of the VEGF promoter The mode of action of SIN-1...
- 8
- 332
- 0
báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
Ngày tải lên :
10/08/2014, 10:20
... SA, Lin Y: A critical role of luteolininduced reactive oxygen species in blockage of tumor necrosis factoractivated nuclear factor-kappaB pathway and sensitization of apoptosis in lung cancer ... types of human cancer cells to cisplatin-induced cell death Importantly, we found that the chemosensitization effect of saikosaponin is mainly mediated by the induction of cellular reactive oxygen ... proposed, including decrease of cellular concentration of the drug, enhancement of drug inactivation due to increased cellular levels of metallothionine and glutathione, increase of DNA repair, and alterations...
- 8
- 395
- 0
Báo cáo y học: " Early The tripeptide feG regulates the production of intracellular reactive oxygen species by neutrophils" pps
Ngày tải lên :
11/08/2014, 08:21
... 100 mg/kg of sensitizing antigen into the stomach by gavage (OA group; n = 25); or (4) 100 μg/kg of feG intraperitoneally, and 100 mg/kg of OA (OA+feG group; n = 25) A dose of 100 μg/ kg of feG ... marker of cell viability, the number of neutrophils were calculated To evaluate the role of PKC on the release of superoxide leukocytes (1 × 106/ml) were preincubated for at 37°C with one of several ... Phagocytosis following translocation of the neutrophil b-cytochrome from the specific granule to the plasma membrane is associated with an increased leakage of reactive oxygen species Scand J Immunol 1992,...
- 9
- 214
- 0
Báo cáo khoa học: "A Reactive oxygen species: toxic molecules or spark of life" docx
Ngày tải lên :
12/08/2014, 23:21
... formation of adrenochromes, which are toxic themselves Of interest, in the first identification of SOD, one of the tests of the activity of the enzyme was the prevention of oxidation of catecholamines ... evaluation of the utility of anti-oxidant therapies What is the reactive oxygen species that is causing the oxidative injury? What is the target molecule? What is the source of the ROS? What types of ... part of the chemistry of life [10,11] Furthermore, evidence of oxidative injury may be the end result of the inflammatory process rather than the major cause of injury, in which case the use of...
- 8
- 226
- 0
Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species
Ngày tải lên :
24/08/2014, 12:59
... Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For the degree of Master of Science Choose your degree I certify that in the preparation of this ... through regulation of nuclear genes, inactivation of STAT3 is shown to disrupt mitochondrial activities and result in an increased level of reactive oxygen species (ROS) Inactivation of STAT3 suppressed ... University, August 2012 Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species Major Professor: Jiliang Li Signal Transducers and Activators of Transcription (STAT3), a transcription factor...
- 73
- 255
- 0
Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
Ngày tải lên :
16/09/2015, 08:31
... Apoptosis Fig C Mitochondial pathway of Apoptosis Fig D Wide array of functions attributed to Reactive Oxygen Species Fig E A model of ROS-mediated regulation of Apoptosis 15 Fig F Various plasma ... modifications of many of the proteins involved in apoptotic machinery (Dechao L et al 2004; John JH, 2004; Irani K and Pascal JGC, 1998) I.2 Reactive Oxygen Species and Apoptosis Cells generate reactive oxygen ... of figures vii List of abbreviations x Chapter I Introduction I.1 Cell Death I.1.a Types of Cell Death I.1.a Programmed cell death or apoptosis I.1.b Apoptotic machinery I.2 Reactive oxygen species...
- 155
- 399
- 0
A study to investigate the involvement of nadph oxidase 5 (NOX5) in resveratrol (RSV) induced reactive oxygen species (ROS) production in u937 cells
Ngày tải lên :
26/09/2015, 10:44
... Modulation of cellular redox status 26 (iv) Autophagy and metabolism (v) RSV and Reactive oxygen species (ROS)/NADPH Oxidase (NOX) 1.5: Aim of study 29 1.6: Mode of action of ... of ROS species NOX enzymes are one of the major sources of ROS NOX5 belongs to the family of NOX enzymes NOX5 differs from the other members of the NOX family in aspects as such as its mode of ... 0.039% carbon dioxide, and small amounts of other gases Air also contains a variable amount of water vapor (an average of 1%) Reactive oxygen species (ROS) are oxygen- derived small molecules (Bedard...
- 110
- 528
- 0
Role of neutrophil NADPH oxidase derived reactive oxygen species (ROS) in innate immune responses
Ngày tải lên :
21/05/2016, 22:38
... dissertation List of tables List of tables Table List of antibodies used for flow cytometry analysis 20 Doctoral dissertation Summary SUMMARY I have investigated the role played by reactive oxygen species ... ROS? ROS include a number of chemically reactive partially reduced forms of oxygen including O2−, H2O2, and HO• These ROS are substantially more reactive than molecular oxygen which is itself a ... reduction of O2 to water without the formation of ROS However, partial reduction of O2, which results in the generation of ROS, can occur if O2 interacts with the electron transport chain upstream of...
- 82
- 267
- 0
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... Role of oxygen- derived free radicals in Helicobacter pylori water extract-induced mouse skin carcinogenesis Biofactors 2006;28: 45-52 Shi GY, Gao F, Shi XG, et al Detection of cellular reactive oxygen ... Fig.10 Mutation of 303PolyC in the mtDNA D-loop region a: Sequence of 303PolyC in the mtDNA D-loop region of AGS cells, the section of black line is the normal sequence; b: Sequence of 303PolyC in ... Sequence of 16184PolyC in mtDNA region of AGS cells, the section of black line is the sequence; b: Sequence of 16184PolyC in mtDNA http://www.medsci.org Int J Med Sci 2011, 64 D-loop region of AGS...
- 12
- 557
- 2
Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine fibrosarcoma L929 cells doc
Ngày tải lên :
18/02/2014, 13:20
... required for the prevention and treatment of many diseases [2] Reactive oxygen species (ROS), a group of highly reactive molecules, including singlet oxygen, hydroxyl radicals, superoxide anion, ... endogenous production of reactive oxygen species Cancer Res 65, 948–956 12 Pan J, She M, Xu ZX, Sun L & Yeung SC (2005) Farnesyltransferase inhibitors induce DNA damage via reactive oxygen species in human ... 500 lL of NaCl ⁄ Pi and 10 mL of 70% ethanol at °C overnight; then, after washing twice with NaCl ⁄ Pi, the cells were incubated with mL of PI staining solution (50 mgÆL)1 of PI and gÆL)1 of RNase...
- 16
- 547
- 0
Báo cáo khoa học: 2-Amino-nonyl-6-methoxyl-tetralin muriate activity against Candida albicans augments endogenous reactive oxygen species production – a microarray analysis study doc
Ngày tải lên :
28/03/2014, 23:20
... 10b and endogenous reactive oxygen species R M Liang et al Results CH3O Response of gene expression to 10b exposure NH(CH2)8CH3.HCl Fig Chemical structure of 10b applicable to psychiatry, ... a series of differentially expressed genes were involved in energy metabolism, oxidoreduction and other biological functions In addition, measurements of endogenous reactive oxygen species (ROS) ... Flash at a wavelength of 550 nm and two wavelengths of 340 nm and 380 nm One unit of complex III activity was defined as the amount of enzyme activity that oxidized lmol of reduced ubiquinone...
- 11
- 336
- 0