sage analysis to identify embryonic stem cell predominant transcripts pdf

... pathways and intermediates between the embryonic stem cell and the mature adipocyte In particular, mouse embryonic stem cells (mESCs) have provided an invaluable tool to model the earliest steps of adipocyte ... evidence that this factor is not sufficient to drive adipocyte commitment in the mESC system, suggesting that more complex regulatory mechanisms are needed to allow immature stem cells to launch adipocyte ... Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonic stem cells and of human adult stem cells into adipocytes Curr Protoc Cell Biol 2007, Chapter 23(Unit 23 24) 54 Irizarry RA, Bolstad...

Ngày tải lên: 09/08/2014, 20:22

... GAPDH 50 µm 20 µm Analysis neural crest stem cell associated transcripts in BMP2+ cells Figure of Analysis of neural crest stem cell associated transcripts in BMP2+ cells (a) RT-PCR analysis for the ... in BMP2+ cells in comparison to control EBs Moreover, to identify BMP2+ cell- specific genes, a three condition comparative analysis of the BMP2+ cells to control EBs and to BMP2 ES cells was ... BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of the protocol...

Ngày tải lên: 14/08/2014, 08:20

... Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells ... Endothelial cells derived from human embryonic stem cells Proc Natl Acad Sci USA 2002, 99:4391-4396 Choi K: The hemangioblast: a common progenitor of hematopoietic and endothelial cells J Hematother Stem ... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic...

Ngày tải lên: 14/08/2014, 08:20

... (version 1.7.0) [18,19] was used to identify transcripts of interest belonging to the biologic process categories adhesion, cell cycle, cell death, cell- cell signaling, cellular metabolism, development, ... Hescheler J, Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic research ... 'cardiomyocyte' cytoskeleton GO categories (for example, myofibril, cytoskeleton, myosin, and actin cytoskeleton) and the 'voltage-gated ion channel activity' GO category were found to be enriched...

Ngày tải lên: 14/08/2014, 20:22

... across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation and gene expression, it is possible to identify cell line-specific ... bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: whole transcriptome shotgun sequencing; ... human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 Huang da W, Sherman BT, Lempicki RA: Systematic and integrative analysis of large gene lists...

Ngày tải lên: 09/08/2014, 23:20

... demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomyocytes, and insulin-secreting cells, to name a few [3-9] Many new ... ongoing studies is to introduce an effective anti-HIV gene into hematopoietic progenitor cells [11] As these cells possess the ability to self-renew, they have the potential to continually produce ... Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human blastocysts:...

Ngày tải lên: 10/08/2014, 05:20

... indicate that if one is to identify signals that cause stem cells to differentiate into specialized cells, work needs to continue with both hES and mES cells Embryonic stem cells have three important ... signaling factors can also be used to induce mES cells to differentiate into specific cell types in vitro, including hematopoietic stem cells, beating cardiac muscle cells, neuronal progenitors, endothelial ... cells) Other stem cells are less committed and retain the potential to differentiate into many types of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and...

Ngày tải lên: 06/03/2014, 15:20

... mouse embryonic stem cells Cell 133, 1162–1174 6668 29 Egli D, Birkhoff G & Eggan K (2008) Mediators of reprogramming: transcription factors and transitions through mitosis Nat Rev Mol Cell Biol ... introduced the vector into Ptb+ ⁄ ) ES cells, which we Table Genotypes of established ES cell lines from blastocysts Fifty-one blastosysts from heterozygous intercrossing were used for ES cell derivation ... flox-hyg; Fig 1B) To generate Ptb) ⁄ ) ES cells, we introduced a Cre expression vector into Ptb) ⁄ flox-hyg cells by electroporation (Fig 1C) Although we obtained Ptb) ⁄ flox cells, we failed to establish...

Ngày tải lên: 07/03/2014, 00:20

... turned out to display a normal karyotype (40 chromosomes) Transcriptome analysis of mES cell lines In order to identify the effects of the overexpression of a single gene on the mES transcriptome, ... generation of mES clones to be added to this cell bank This opens the possibility of using this system to study other aneuploidy disorders in which the gene dosage imbalance seems to be the main cause ... tet-inducible system for massive overexpression of transcription factor genes in mouse ES cells that it is indeed possible to identify their physiological function from transcriptome analysis We...

Ngày tải lên: 09/08/2014, 20:22

... Solter D: From teratocarcinomas to embryonic stem cells and beyond: a history of embryonic stem cell research Nat Rev Genet 2006, 7:319-327 Srivastava D, Ivey KN: Potential of stem- cell- based therapies ... Enrichment analysis of functional groups within the stem cell- derived cardiopoietic transcriptome Enrichment analysis of functional groups within the stem cell- derived cardiopoietic transcriptome (a) ... properties were ascribed to each network node mapping respective contributions to the overall execution of cardiopoietic transformation of embryonic stem cells Discussion Embryonic stem cells are developmentally...

Ngày tải lên: 14/08/2014, 08:20

... regulatory circuitry in human embryonic stem cells Cell 2005, 122:947-956 Kim J, Chu J, Shen X, Wang J, Orkin SH: An extended transcriptional network for pluripotency of embryonic stem cells Cell ... sustaining factor in embryonic stem cells Cell 2003, 113:643-655 Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, Gifford DK, Melton DA, Jaenisch ... Biology 2008, Melton DA, Gifford DK, Jaenisch R, Young RA: Control of developmental regulators by Polycomb in human embryonic stem cells Cell 2006, 125:301-313 Lee TI, Johnstone SE, Young RA:...

Ngày tải lên: 14/08/2014, 20:22

... Ecosystem Science 5:11–20, 2013 C American Fisheries Society 2013 ISSN: 1942-5120 online DOI: 10.1080/19425120.2012.741558 ARTICLE Application of Morphometric Analysis to Identify Alewife Stock ... freshwater for to months (Richkus 1975) until they out-migrate to the ocean from late summer to late fall (Iafrate and Oliveira 2008; Gahagan et al 2010) Alewives are believed to return to their natal ... (FL) and total length (TL) were measured to the nearest millimeter and total mass was recorded to the nearest gram After a standardized digital image was recorded, fish were dissected to confirm...

Ngày tải lên: 04/09/2015, 08:12

... pluripotent stem cells into specific neural cell types requires exposure of the cells to the right culture conditions (an optimal concentration of growth factors, signaling molecules,   7  inhibitors, ... pluripotent stem cells H1 hESCs (passage number 20 to 35) and a human iPS cell line (passage number to 20) were grown feeder-free on Matrigel (BD Biosciences), cultured in mouse embryonic fibroblast ... control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded transcriptional regulatory circuit...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... 35 lncRNAs were found to be highly upregulated in hESC-derived neurons, as compared to the undifferentiated embryonic stem cells or the neural progenitors (Figure 5.2F) To identify functional lncRNAs ... to stem cell pluripotency (Pereira et al., 2010; Walker et al., 2010) Taken together, this indicate that lncRNAs are intricately woven into the core transcriptional circuitry governing stem cell ... very low To validate that the pluripotency lncRNAs are bona fide non-coding transcripts, the Coding Potential Calculator (CPC) tool was employed to predict protein-coding potential of the transcripts, ...

Ngày tải lên: 09/09/2015, 17:54

... factor, is a transcription factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem ... RMST knockdown in ReN-VM neural stem cells (Figures 7.4, 7.5 and 8.7) Knockdown of RMST appeared to shut down the neurogenic program of neural stem cells, with the cells adopting a glia lineage ... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated...

Ngày tải lên: 09/09/2015, 17:54

... L., Landeira, D., Santos, J., Banito, A., Gil, J., et al (2010) ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency Cell Stem Cell 6, 547-556 Perrier, ... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... cells to pluripotency Cell Stem Cell 8, 376-388 Anthony, T.E., Klein, C., Fishell, G., and Heintz, N (2004) Radial glia serve as neuronal progenitors in all regions of the central nervous system...

Ngày tải lên: 09/09/2015, 17:55

... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... dendritic cell Myeloid progenitor cells Mesenchymal stem cell Natural killer T cell Neural stem cell Poly(2-hydroxyethylmethacrylate) Protein phosphatase 1, regulatory subunit 12C Pluripotent stem cell ... Hematopoietic progenitor cell Hematopoietic stem cell Herpes simplex virus Interferon- Immunoglobulin Interleukin Induced pluripotent stem cell Macrophage colony-stimulating factor Major histocompatibility...

Ngày tải lên: 09/09/2015, 18:52

... progenitor cell Homeodomain Human embryonic stem cell High mobility group Herpes simplex virus thymidine kinase Interferon Interleukin Induced pluripotent stem Luria broth Leukemia inhibitory factor ... differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable ... factor Tailess Untranslated region Vascular endothelial growth factor Ventricular zone xii Chapter 1: Introduction 1.1 Neural stem cells 1.1.1 A brief history of NSCs The field of neural stem cell...

Ngày tải lên: 10/09/2015, 09:08

... five human ES cell lines by Thomson et al from the B ES cells A Embryonic Development Figure Contribution of the blastocyst inner cell mass to embryonic development and embryonic stem cells (A) The ... Derivation of embryonic stem cells The inner cell mass (ICM) of an embryonic blastocyst is a source of pluripotent cells that ultimately give rise to the embryo proper Following implantation into the ... maintenance of pluripotency in embryonic stem cells 246 vi ABSTRACT The transcription factors Oct4, Nanog and Sox2 are key regulatory players in embryonic stem (ES) cell biology Dissecting...

Ngày tải lên: 11/09/2015, 09:02