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... a mediator of inflammation, atherosclerosis, and cancer [43-45] Several in vitro studies have suggested 12/15-LOX products to be co-activators of peroxisomal proliferator activating-receptors ... purchased from R&D (Minneapolis, MN) RPMI 1640 media and HEPES were obtained from GibcoBRL (Rockville, MD) 2-mercaptoethanol was purchased from Sigma (St Louis, MO) Fetal bovine serum was obtained from ... 5B) Role of IkBα proteins in the inhibitory effect of BAI on inflammatory cytokine production from IL-1β- and TNF-αactivated mast cells The activation of NF-κB requires phosphorylation and proteolytic...

Ngày tải lên: 13/08/2014, 13:22

... biological processes in airway cells It has been shown that protease-activated receptor (PAR)-2 functions as a target protein for HAT in bronchial epithelial cells [5] PAR-2 is a member of the PAR protein ... to mucus production by the airway epithelial cell line (NCI-H292) in a range of samples obtained from the sputum of patients with chronic airway disease such as bronchial asthma or bronchitis ... induce AR protein production, but cannot account for AR release Thus, the mechanism of HAT-induced AR release from a cell was also investigated The effect of GM6001, a broadspectrum metalloprotease...

Ngày tải lên: 30/03/2014, 11:20

... Y: Increased C-reactive protein and increased plasma interleukin-6 may synergistically affect the progression of coronary atherosclerosis in obstructive sleep apnea syndrome Circulation 2003, 107:E40-40 ... lymphocytes and neutrophils Liu et al demonstrated an increase of lymphocyte and neutrophil cells after acute sleep deprivation [17] As inflammatory processes are recognized to play a major role in the ... Prolo P, Lin HM, Vela-Bueno A, Kales A, Chrousos GP: Chronic insomnia is associated with a shift of interleukin-6 and tumor necrosis factor secretion from nighttime to daytime Metabolism 2002,...

Ngày tải lên: 20/06/2014, 00:20

... the growth of ed breast tumor xenografts One mouse in the control group died on day 13 and one mouse in the UTI group died on day 18 due to consumption and cachexia The tumors in the control group ... treatment groups versus control; b P < 0.01 for TXT and UTI+TAX groups versus UTI group; Discussion Ulinastatin (UTI) is a serine protease inhibitor (SPI) with extensive inhibitory effects on cell proliferation ... UTI and TAX on IL-6 protein expression in human breast cancer xenografts in immunohistochemistry: Control group SP × 400 UTI group SP × 400, TAX group SP × 400 UTI+TAX group SP × 400 Figure Effects...

Ngày tải lên: 10/08/2014, 10:21

... exert protective effects Because of its SH group, NAC scavenges H2O2 (hydrogen peroxide), •OH (hydroxyl radical), and HOCl (hypochlorous acid) In addition, NAC reduces cellular production of pro-inflammatory ... polymerase (Invitrogen) in a total reaction volume of 50 µL PCR products were electrophoresed on 1.2% agarose gel containing 0.1% ethidium bromide Positive and negative controls were run concurrently ... the production of NOx from A549, mouse Effects of CSS on the production of NOx from A549, mouse AEC, and NHTBE Cells were incubated with CSS for 24 h (AEC and NHTBE) or 48 h (A549) NOx production...

Ngày tải lên: 12/08/2014, 18:22

... macrophages located in the sinusoids of the pseudolobules SAB method ×13 Fig Mast cells (arrow head) and myofibroblasts (arrow) are simultaneously detected within the fibrous septa (F) and around ... relationship and role of macrophages, myofibroblasts and mast cells in the hepatic septal fibrosis induced by C hepatica We hypothesize that a similar mechanism involving macrophages, myo-fibroblasts ... myofibroblasts and mast cell-released fibrogenic cytokines (platelet-derived growth factor and transforming growth factor-beta) might be involved in the progress of hepatic inflammation, fibrosis...

Ngày tải lên: 07/08/2014, 20:23

... test – CPM of medium control)/(CPM of Maximum control – CPM of medium control) ×100, where medium control was measured in medium alone without effector cells and maximum control was counted in 0.2 ... HLA-DR From 16 February 2007, we drained 3000 ml of ascites from the hypogastric area every week for month A total of 13,222 ml of ascites was aspirated from this area (Figure 1a) Then, from 23 ... count (1/77) was observed: from 4.6 × 108 cells to × 106 cells From 23 March (day 36), we initiated drainage from the epigastric area The number of tumor cells decreased from 6.6 × 108 (day 36) to...

Ngày tải lên: 11/08/2014, 19:21

... Kanai et al was shaken gently at room temperature for 30 to allow binding of the recombinant proteins to the S-protein agarose through their N-terminal S-tags The agarose beads were washed three ... 3111–3120 38 Kiefer SM & Saling P (2002) Proteolytic processing of human zona pellucida proteins Biol Reprod 66, 407– 414 39 Cross NL & Watson SK (1994) Assessing acrosomal status of bovine sperm using ... H, Staehelin T & Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc Natl Acad Sci USA 76, 4350–4354 55...

Ngày tải lên: 07/03/2014, 05:20

... the protein to assemble into or disassemble from microtubules Kinetics of release of 3-nitrotyrosine from nitrotyrosinated tubulin Two carboxypeptidases were used to study release of nitrotyrosine ... [14C]nitrotyrosine or [14C]tyrosine into tubulin, the protein preparations were processed to compare the abilities of tyrosinated and nitrotyrosinated tubulin to assemble into and to disassemble from ... tyrosine was substituted by 3-nitrotyrosine The possibility that the disappearance of nitrotyrosinated tubulin that occurred after elimination of nitrotyrosine from culture medium was due to protein...

Ngày tải lên: 17/03/2014, 10:20

... TRIzol reagent were from Life Technologies Horseradish peroxidase-conjugated antigoat and antirabbit IgGs were from Zymed A23187 was from CalBiochem Human IL-1b was obtained from Genzyme Construction ... cells by a nuclear factor jB and peroxisome proliferator-activated receptor-mediated process J Biol Chem 274, 23085–23093 60 Crowl, R.M., Stoller, T.J., Conroy, R.R & Stoner, C.R (1991) Induction ... Goddeau, R P., Rogers, R.A., Austen, K.F & Arm, J.P (1999) Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived...

Ngày tải lên: 18/03/2014, 01:20

... followed by the appropriate secondary antibody (Alexafluor, InvitrogenMolecular Probes, Eugene, OR, USA) each for h Coverslips were mounted and viewed by confocal microscopy (Leica Microsystems, Mannheim, ... results strongly indicate that, in spite of the structural similarities with ricin A chain, saporin and derived chimeras follow a different intracellular transport route(s) Results Vero and HeLa ... of a pore through which the catalytic chain escapes into the cytosol and inactivates protein synthesis [27,28] Therefore, in both Vero and HeLa cells, we analyzed the effects of chloroquine, a...

Ngày tải lên: 23/03/2014, 15:21

... which belong to the steroid/thyroid receptor superfamily [7–9] Several studies indicate that protein–protein interactions between nuclear retinoid receptors mediate cellular cross-talk, thus generating ... reporter construct, NFAT(FasL)-Luc, in which an NFAT binding site from the FasL promoter was subcloned upstream of a heterologous SV40 promoter and luciferase When this construct was transfected into ... A 100-fold excess of unlabeled probe or of an unlabeled oligonucleotide encompassing the NFAT binding sequence from the IL-2 promoter, completely abolished the protein– DNA complexes, whereas...

Ngày tải lên: 24/03/2014, 03:21

... contribute to the progression of colon cancer Page of pressure cooker treatment in citrate buffer (pH 6.0) for minutes Then endogenous peroxidase was blocked with 3% hydrogen peroxide incubation ... stroma of a primary tumor is denoted as MCC stroma The stained sections were first screened under lower power (×100) to identify the areas with the most mast cells in the tumor stroma MCCstroma ... node without metastasis (MCC lnwm ) were evaluated as MCCstroma All evaluated section were obtained from areas far from the area of necrosis and H E staining was reviewed in uncertain cases The...

Ngày tải lên: 18/06/2014, 19:20

... purposes) Virology Journal 2008, 5:110 http://www.virologyj.com/content/5/1/110 Figure TULV inhibits ERK1/2 cell survival pathway in Vero E6 cells TULV inhibits ERK1/2 cell survival pathway in Vero E6 ... cell death [26] Besides regulating apoptosis, ERK proteins have other essential roles in the kidneys They promote tubular epithelial cell proliferation [27,28] and epithelial cell barrier resistance ... contrast, some viral proteins, like Ebola virus glycoprotein [22], hepatitis C virus non-structural protein NS5A [23], and human immunodeficiency virus (HIV) type vpr protein [24] have been shown...

Ngày tải lên: 20/06/2014, 01:20

... contribute to the progression of colon cancer Page of pressure cooker treatment in citrate buffer (pH 6.0) for minutes Then endogenous peroxidase was blocked with 3% hydrogen peroxide incubation ... stroma of a primary tumor is denoted as MCC stroma The stained sections were first screened under lower power (×100) to identify the areas with the most mast cells in the tumor stroma MCCstroma ... node without metastasis (MCC lnwm ) were evaluated as MCCstroma All evaluated section were obtained from areas far from the area of necrosis and H E staining was reviewed in uncertain cases The...

Ngày tải lên: 20/06/2014, 03:20

... Vergnolle N, Guiard B, Fioramonti J, Bueno L Proteinases and proteinase-activated receptor 2: a possible role to promote visceral hyperalgesia in rats Gastroenterology 2002, 122, 1035-1047 10 Corvera ... syndrome Gastroenterology 2001, 120, 652-668 Celik AF, Demirkesen C, Pamuk ON, Pamuk GE, Uzunismail H Mast cells: they really have a role in disturbed bowel habits of IBS patients? Am J Gastroenterol ... Neurogastroenterol Motil 1998, 10, 499-508 19 Mertz H, Naliboff B, Munakata J, Niazi N, Mayer EA Altered rectal perception is a biological marker of patients with irritable bowel syndrome Gastroenterology...

Ngày tải lên: 07/08/2014, 18:20

... proteins (PER1 and PER2) and Cryptochrome proteins (CRY1 and CRY2) act as negative regulators of transcription driven by the BMAL1/CLOCK heterodimer Therefore, E-box elements play a crucial role ... the transmembrane electrochemical proton gradient in mitochondria to improve the efficiency of the proton-motive force and generate greater calcium release by an antiport process.26 A number of ... plays an important role in the control of the differentiation of MSCs We propose that blue laser irradiation of MSCs could provide a simple and effective technology for clock protein localization...

Ngày tải lên: 08/08/2014, 17:20

... histamine; 5HT2A = serotonin 2a; NGF = nerve growth factor; NK-1 = neurokinin 1; PAR = protease-activated receptor; TNFR = tumour necrosis factor receptor; trk = neurotrophin tyrosine kinase receptor ... clear that classic neurotransmitters such as acetylcholine and neuropeptides are produced by nonneuronal cells Nonneuronal cells of the immune system, such as monocytes, macrophages, T lymphocytes, ... neuroimmunology Autonomic Neuroscience Series Vol 15 London, (UK): Taylor&Francis group; 2003 p 139–170 Purcell WM, Atterwill CK Mast cells in neuroimmune function: neurotoxicological and neuropharmacological...

Ngày tải lên: 08/08/2014, 20:23