roles of security manager in an organization

... alanine scanning of the L-CDR3 and H-CDR3 loops of antibody mAb4E11. This scanning allowed us to identify the residues of these loops that contributed to the energetics and kinet- ics of the interaction ... systematic scanning of the CDR3 loops of mAb4E11 by mutagen- esis of their residues into alanine (Ala scanning). The affinities of the purified mutant Fab fragments of mAb4E11 for its antigen were ... Thorn KS (1998) Anatomy of hot spots in protein interfaces. J Mol Biol 280, 1–9. 32 DeLano WL (2002) Unraveling hot spots in binding interfaces: progress and challenges. Curr Opin Struct Biol...

Ngày tải lên: 19/02/2014, 07:20

... exam- ine the roles of the conserved arginines, Arg322 (P2) and Arg323 (P1) of AAA-1, and Ar g747 (P1) of AAA- 2, and determined that the arginines of TClpB are not involved in nucleotide binding ... these three arginines in TClpB. By combining an arginine mutation with the WalkerA mutation in the other AAA+ module, we tested the roles of the arginines in ATP binding and hydrolysis in each module. ... arginines are not involved in nucleotide binding To examine the roles of conserved arginines, we gener- ated three mutants of TClpB: R322A [1R ⁄ A(P2)], R323A [1R ⁄ A(P1)], and R747A (2R⁄ A). In...

Ngày tải lên: 06/03/2014, 00:20

... Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN, USA 2 School of Informatics, Indiana University – Purdue University Indianapolis, IN, USA 3 Laboratory of Statistical ... interaction with Cdk2 and cyclin A: 1, domain 1 interacting with cyclin A; 2, a linker helix involved in binding both cyclin A and Cdk2; 3, domain 2 inter- acting with Cdk2 [122]. Blue and dark yellow horizontal ... capable of bind- ing many different partners. An interesting consequence of the capability of ID proteins and regions to interact with different binding partners is the potential for polymorphism in...

Ngày tải lên: 07/03/2014, 21:20

... one-third of all solid tumors [20]. During cancer progression, increased shedding of the membrane-anchored ligands of EGFR, including heparin-binding EGF (HB-EGF), transforming growth factor (TGF)-a and ... Shedding of membrane-anchored ligands of EGFR (HB-EGF, TGF-a and amphiregulin) ADAM10 Shedding of E-cadherin MMP-9, -2, -14 Activation of TGF-b MMP-7 (anchored to CD44) Shedding of HB-EGF Cancer ... their main roles focus on ectodomain shedding and nonpro- teolytic functions, such as binding to adhesion mole- cules, integrins and interacting with phosphorylation sites for serine ⁄ threonine and...

Ngày tải lên: 15/03/2014, 00:20

... of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. 40 Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of ... PEI and PKI mutants were obtained by replacing Arg89 of GSTU1 (REI) with Pro89, and Glu117 of the PEI mutant with Lys117, respectively. Steady-state kinetic analysis of purified RKI and RKV mutants showed ... induction of distinct glutathione transferases of wheat by xenobiotics and by pathogen attack. Plant Physiol 102, 1193–1201. 17 Dean JD, Goodwin PH & Hsiang T (2005) Induction of glutathione S-transferase...

Ngày tải lên: 15/03/2014, 09:20

... of alanine or glutamine for Glu182 blocks binding of PDP1 and PDP1c to the L2 domain. As can be seen in Fig. 2A, a distinct pocket exists in the center of this set of residues. Binding of PDP1c-Ca 2+ to ... higher rates of phosphorylation of the E2-bound E1. Binding of a kinase dimer to a lipoyl domain containing a reduced lipoyl group fosters a further increase in kinase activity (PDK* state). Binding of ... by the L1 domain of E2 and L3 domain of E3BP (Fig. 1) and reduction and acetylation of the lipoyl groups of these domains enhance PDK4 activity. PDK1 binds to the L1 and L2 domains of E2 (Fig....

Ngày tải lên: 17/03/2014, 09:20

Ngày tải lên: 23/03/2014, 04:21

... pattern emerging in a sequence of numbers; a point of coincidence found in a group of lines; a large change resulting from a small variation; a finite real thing proved by an infinite, possibly ... particularly attuned to things analogous, he also wants to and enjoys finding analogies—they are aesthetically pleasing to him. The wealth of analogies evident in his initiation into triangle geometry satisfies ... playing is aesthetic insofar as the mathematician is framing an area of exploration, qualitatively trying to fit things together, and seeking patterns that connect or integrate. Rather than being...

Ngày tải lên: 30/03/2014, 16:20

... reported incidence of 3-4% in the adult population [1-3]. Interestingly, it is extremely rare in Africa and Asia, and rarely occurs in Japanese individuals [3-6]. Although the etiology of Paget's ... Guidelines for diagnosis and management of Paget's disease of bone in Japan. J Bone Miner Metab 2006, 24:359-367. 7. Kanis JA: Pathophysiology and treatment of Paget's disease of bone ... and KS have been involved in drafting the manuscript. All authors read and approved the final manuscript. Author Details 1 Department of Orthopaedic Surgery, Gifu University Graduate School of...

Ngày tải lên: 20/06/2014, 04:20

... patient and wrote the manuscript. MS and TA participated in writing and design of the manuscript. They also drafted the manuscript. All authors read and approved the final manuscript. Conflict of interest ... M, Oka Y: Cystic lesion in carpal bone. Hand Surg 2000, 5:25-32. 6. Athanasian EA: Aneurysmal bone cyst and giant cell tumor of bone of the hand and distal radius. Hand Clin 2004, 20:269-81. 7. ... [3]. The main indication for surgical inter- vention is to prevent or treat a pathological fracture [1,3]. In our case herein, in dications for surgery were clini- cal history of pain and radiographic...

Ngày tải lên: 20/06/2014, 04:20

... capacity of management and control of behaviors of individuals and organizations in economic activities with the aiming of protecting and recovering the ecological environment. In terms of methodology, ... plays an important role in issuing the undertakings, policies, laws and organizing, building the team of professional staff that is in charge of performing the management of resources and environment. ... team of professional staff by planning, training and developing skilled staff and Government officials. Then, there will be a team of skilled staff, ensuring the continuing hierarchy in terms of...

Ngày tải lên: 14/07/2014, 13:34

... of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. 40 Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of ... PEI and PKI mutants were obtained by replacing Arg89 of GSTU1 (REI) with Pro89, and Glu117 of the PEI mutant with Lys117, respectively. Steady-state kinetic analysis of purified RKI and RKV mutants showed ... induction of distinct glutathione transferases of wheat by xenobiotics and by pathogen attack. Plant Physiol 102, 1193–1201. 17 Dean JD, Goodwin PH & Hsiang T (2005) Induction of glutathione S-transferase...

Ngày tải lên: 07/08/2014, 01:20

... packings, the distance of the disk center to the boundary of this enclosing triangle is the distance to the line between bonds with the left side of the triangle and disk 24, and is 0.0317185 of ... compute and report d(n)to15significantdecimal digits. Disk packings in equilateral triangles differ from those in squares or circles in that for triangles there are an in nite number of values of n ... absence of gravitation and friction, the disks move along straight lines, colliding with each other and the region walls according to the standard laws of mechanics, all the time maintaining a...

Ngày tải lên: 07/08/2014, 06:20

... mean height of individual Holm Tab le I. Mean and standard deviation (SD) of canopy height, number of stems and basal area for the 900 1-m 2 sampling points of the study plot, and mean and standard ... (tree- and shrub-dominated zones) are indicated on the map. Distances shown in the axes are in m. vegetation in many current Mediterranean ecosystems, where abandoned woodlands and shrublands develop ... Madrid, Spain. Mean elevation of the zone is 640 m a.s.l. and it experiences a dry, continental, Mediterranean weather with a mean annual tempera- ture of 14.8 ◦ C and an annual precipitation of 420...

Ngày tải lên: 07/08/2014, 16:20

... Statsoft Inc., STATISTICA (data analysis software system), version 6, Statsoft Inc., Tulsa, 2001. [59] Stieperaere H., Fransen K., Standaardlijst van de Belgische vaatplanten, met aanduiding van ... variables in the Pteridium stand. Harrow did not have any effect on the considered soil parameters. Both disc and rotary ploughing induced an overall lowering of soil nutrient index and soil compaction, ... trails and haul roads on understory plant richness and compo- sition in management forest landscapes in Upper Michigan, USA, For. Ecol. Manage. 175 (2003) 509−520. Soil mechanical treatments in...

Ngày tải lên: 07/08/2014, 16:20

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Ngày tải lên: 10/08/2014, 22:20

Ngày tải lên: 10/08/2014, 23:20