role of the chemical structure on the properties

... show the critical role of the last amino acids in the C-terminus of MaL Deletion of the four last amino acids strongly reduced the expression of the protein in T reesei and S cerevisiae Furthermore, ... change in the C-terminus of MaL (L559A) changed the catalytic properties of the laccase The solved three-dimensional structure of the protein with the L559A mutation revealed conformational changes ... and determined the inhibition constants for sodium azide, in order to see the effect of the mutation (L559A) on the binding properties in the trinuclear center The inhibition of MaL by azide...

Ngày tải lên: 16/03/2014, 00:20

... region of the C-tail of the D1 receptor might be involved in the interactions between the two dopamine receptors In addition, the subcellular localization of D1–CFP, D2–YFP and all the mutants of ... and their interactions with the appropriate a subunits of G protein, further confirm that the use of advanced fluorescence techniques does indeed allow for the observation of true interactions The ... residues in the C-tail of the D1 receptor The efficiency of energy transfer was reduced to 0.8% A possible interpretation of the data suggests that the indicated basic region of ic3 of the D2 receptor...

Ngày tải lên: 07/03/2014, 03:20

... isolation The “what, whys, and hows” related to changing the role of the auditor has to be considered within the context of the overall financial reporting system, including the important roles of the ... to convene investors and other financial reporting stakeholders to examine the role of the auditor and the value of the audit On June 21 the PCAOB issued a concept release on potential revisions ... recommendations This report concludes with identification of the CAQ’s short and longer-term next steps to further pursue observations regarding the role of the auditor Appendix A to this report contains...

Ngày tải lên: 15/03/2014, 20:20

... allows the interaction of the enzyme with the substrate structure Whether the motion of the lid promotes the recognition of the substrate structure, or the recognition of this structure promotes the ... presence of the Cc domain and bile salt only in the presence of the C2 domain The inhibition of the active site serine by E600 needs both the motion of the flap and the recognition of the vehicle ... important information concerning the structure function relationship of the PL [24–28] In agreement with biochemical data, these structures demonstrate the functional organization of the PL into two...

Ngày tải lên: 16/03/2014, 06:20

... contrasting reports about the interaction of the 4Â-PP group with the polypeptide backbone and its effect on the stability of holo-ACP The average major conformation of the holo-ACP NMR structure was analyzed ... elucidates the interactions responsible for the conformational stability of PfACP The size exclusion chromatography prole of PfACP showed that the apparent molecular mass of PfACP monomer is 25 ... between the two proteins (as indicated by the values of DGwater, Cm and Tg) hence arise from the changes in the surface of the protein because of the interactions of the cofactor with the protein...

Ngày tải lên: 16/03/2014, 10:20

... maintaining the conformation of the whole PBD and modulating the interdomain interface These findings, together with comparison of the thermal stability, trypsin accessibility and stimulation by co-chaperones, ... exchange at the PBD of DnaK did not modify the overall secondary structure of the chimeras The intrinsic fluorescence of DnaK has been widely used to follow allosteric conformational changes upon nucleotide ... nucleotide the emission maximum of mtHsp70 is downshifted by 5–6 nm with respect to that of DnaK The twofold reduction of the KSV values estimated for mtHsp70 both in the absence and the presence of...

Ngày tải lên: 16/03/2014, 22:20

... proposition of a text The DT best represents the redundancy structure of the text As such, this proposition functions as a reference point for processing the rest of the propositions The text position ... appear at the end of the review (instead of the beginning of the review) as a means of recapitulating the point of the message, thereby guaranteeing that it is fully understood by the readership ... than the first one (contrary to topic-extraction) Based on preliminary investigations, we suggest that the DT of any customer review is the customer’s evaluation, whether negative or positive, of...

Ngày tải lên: 23/03/2014, 16:20

Ngày tải lên: 28/03/2014, 13:20

... localization of the fusion protein correlates not only with activation of FRM2 transcription, but also with growth in increasing concentrations of cadmium but not of H2O2 Because of the high degree of ... the formation of an intermolecular bond occurs between the Orp1 Cys36 and the Cys598 of Yap1 leading to its activation The subsequent formation of the Yap1 Cys303-Cys598 disulfide bond masks the ... Induction of the Msn2 ⁄ target genes in response to one form of stress gives rise to the phenomenon of cross-protection against an aggravated form of the same stress or to a different type of environmental...

Ngày tải lên: 30/03/2014, 16:20

... groups The SEM micrographs also confirm the presence of the attached groups on the outer walls of MWCNTs The presence of the different chemical groups (-COOH, -SO H, and -PO3H2) on the walls of the ... studies The extent of functionalization with different groups has been determined from the EDX results and chemical routes which results in the identification of sulfonation and phosphonation of higher ... and double-step chemical routes The effect of dispersion using Triton X-100 via ultrasonication, before the functionalization of CNTs, has also been studied The defect concentration has been determined...

Ngày tải lên: 20/06/2014, 22:20

... contribution The present investigation was also limited by the fact that the standing rearfoot angle was the only structural measurement of the foot For instance, the range of motion (ROM) of ... part of the stance phase There appeared to be no relationship between the static anatomical structure of the rearfoot with the magnitude of the change in rearfoot kinematics following fatigue Contrary ... in a bid to further understand the role of this specific muscle on foot kinematics The main finding of this investigation was the significant increase in rearfoot eversion excursion during postfatigue...

Ngày tải lên: 10/08/2014, 21:24

... Chapter 24, The biosynthesis of Amino Acids) Glutathione conjugation is one of the systems in the detoxification of many anticancer drugs The major components of this system include glutathione (GSH), ... mediate the transport of the glutathione conjugates LTC4 and DNP-SG, the endothelin receptor antagonist BQ123, but not the glucuronate conjugate E217ßG or cyclic nucleotides The ability of MRP6 ... which suggest that they might involved in the regulation of the intracellular concentration of these important second messengers The cyclic nucleotides, cGMP and cAMP are second messengers involved...

Ngày tải lên: 05/10/2015, 13:53

... along the gradient of environmental conditions from the northern to the southern Red Sea?  How the soft coral communities respond under differing conditions of impact from different pollution ... adaptation of the holobiont towards environmental conditions Barneah et al (2004) reported that the vertical transmission belongs to Symbiodinium clade A while horizontal transmission belongs to the ... estimation of the average annual environmental condition The study sites of Sawall et al (2014) were the same as in this study Additionally the silicate concentration was determined from the same...

Ngày tải lên: 13/05/2016, 10:16

Ngày tải lên: 25/10/2013, 05:20

... [26] These interactions have been reported to induce the disruption of cell–cell junctions, a loss of cell polarity and the induction of pro-inflammatory and mitogenic responses (Fig 3C) The first ... in the amino-terminal region of CagA [28] In particular, CagA induces the ubiquitination and degradation of RUNX3, thereby extinguishing its ability to inhibit the transcriptional activation of ... alteration of host-cell transcription signalling pathways Another important consequence of phosphorylationindependent CagA interactions in polarized epithelial cells is the disruption of cell–cell...

Ngày tải lên: 14/02/2014, 19:20

... focus on measuring the functioning of the financial system rather than on examining the direct impact of financial sector policy, regulations, and the rest of the enabling environment on economic ... Brothers marked the onset of the largest global economic meltdown since the Great Depression The aftershocks have severely affected the livelihoods of millions of people around the world The ... external contributions Note that The World Bank does not necessarily own each component of the content included in the work The World Bank therefore does not warrant that the use of the content contained...

Ngày tải lên: 16/02/2014, 10:20

... two activities, the hydroxylation of monophenols and the oxidation of o-diphenols Different names are used for these activities, as shown in the Figure One of the most common monophenolic substrates ... to be part of the defense system against wounding and infection by the pathogen [16] Thus, the timing and location of melanization seems to be essential for the prevalence of one of these two ... 1.10.3.1), but the differentiation between these two types of enzyme is not clear [4] Looking at the sequences of the two enzymes, both show absolute conservation of the histidine residues of the CuA...

Ngày tải lên: 19/02/2014, 07:20

... complex at the position of the histochemical stain in the left panel Antisera against the SDHC subunit of complex II and against the Rieske protein of complex III revealed the presence of these complexes ... and the effect of the absence of intact complex I, but the results are too preliminary in this regard Heterologous expression of the ESSS-HA subunit The localization of the ESSS subunit in the ... with the incorporation of the tagged ESSS subunit into a functional complex I (Fig 3B, right panel) The signal from the 18 kDa subunit of complex I (NDUFB6) served as another identification of the...

Ngày tải lên: 19/02/2014, 16:20

... The solution structure of the SEA domain of the mouse homologue of MUC16 (which in the human encodes the core protein of the ovarian cancer antigen CA125) was recently solved by multidimensional ... side of the loop, faces solvent and its mutation to alanine has little if any effect on the cleavage event On the other side of the loop, the valine residue (V189) that interacts only with the ... cleavage than are the 33 amino acid substitutions Another feature of the hybrid mucins is that that they lack motifs of the native MUC3 and MUC12 that may contribute to the generation of the cleavage...

Ngày tải lên: 19/02/2014, 18:20

... information about the mode of action and the availability of a refined three-dimensional model [16] prompted us to prepare a series of synthetic fragments designed on the basis of the secondary structure ... results obtained by other groups who also point to the role of the connecting loop of the b-hairpin of defensins A combination of mutational analysis [35] and structural analysis of the plant defensin ... plotted against the log of the measured MIC The aforementioned results showed that synthetic peptides corresponding to fragments of the MGD1 defensin reproduced the behaviour of the entire molecule...

Ngày tải lên: 20/02/2014, 11:20