role of polymeric additives and composite particles in slurries
Báo cáo khoa học: Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome docx
... simultaneous incubation of proteasome preparations with cardiolipin and Grx2 The results obtained showed 25% and 65% increased chymotrypsin-like activity and 61% and 100% increased postacidic cleavage of ... regulatory role in 20S PT activities We next analyzed Grx2 fragmentation using mass spectrometry, by incubating Grx2 in standard buffer for 30 or h in the presence of n-PT After incubation, standard ... decreased intensity of Grx2 bands as evaluated by measurement of optical density When incubated in standard buffer, n-PT was able to degrade about 70% of Grx2 C Fig Degradation of Grx2, Trx1 and Trx2...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing ... visualized the mineralizing cells with 2% Alizarin Red After rinsing in distilled water and air drying the samples, we mounted the coverslips on microscope slides using Fluoromount and examined and photographed...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
... BMP-2 maintained the mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc ... FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related gene expression Mineralizing ... visualized the mineralizing cells with 2% Alizarin Red After rinsing in distilled water and air drying the samples, we mounted the coverslips on microscope slides using Fluoromount and examined and photographed...
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Báo cáo y học: "Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGF-1 in cultured rat nucleus pulposus cells" ppsx
... retinoblastoma protein [26] CDK inhibitors (CKIs) are the negative regulators and are grouped into two families [27] The INK4 family (p15, p16, p18, p19 and p20) only bind and inactivate cyclin ... (p21, p27, and p57) show broader substrate specificity inactivating both cyclin D-CDK4/6 and cyclin ECDK2 kinase complexes [28] We examined the expression of p15INK4, p21WAF1/Cip1 and p27Kip1, ... quantifying DNA Effects of inhibition of c-Myc transcriptional activity and inhibition of ERK1/2 activity in the presence of ng/mL TGF1 were determined After serum deprivation, 79.0% of nucleus...
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Báo cáo y học: " Role of miR-1 and miR-133a in myocardial ischemic postconditioning" pptx
... 031879.1) and AMOs (AMO-1 and AMO-133a) were synthesized by Jima Inc (Shanghai, China) The sequences of miRNA mimics and AMOs are showed in Table Mimic and AMO of miRNA pretreatment in vivo Mimic and ... used to determine relative quantitation of gene expression of both target and housekeeping genes (bactin) The primers of miRNAs and apoptosis-related genes (Bcl-2, Bax and CASP9) used in the study ... lipofectamine 2000 (Invitrogen) 48 h before IR Flow cytometry analysis of apoptosis by annexin V/PI staining Neonatal cardiomyocytes were stained by annexin V/PI as previously described [24], and...
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Báo cáo y học: "The role of mast cells and fibre type in ischaemia reperfusion injury of murine skeletal muscles" pot
... obtained by counting a minimum of 100 mast cell profiles in tongue, skin and heart as well as skeletal muscle Using 100 X magnification all mast cell profiles that fell within a grid area of 1.35 ... muscle stained with Haematoxylin and Eosin Gastrocnemius muscle stained with Haematoxylin and Eosin Control (A & C) and IR treated hind limbs (B & D) from littermate control (A & B) and Wf/Wf ... 24 h in 10% BFS, washed in 0.1 M phosphate buffered saline (PBS) and processed into paraffin Five micron sections were cut, dewaxed and stained with Haematoxylin and Eosin (H&E) for general analysis...
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Báo cáo y học: "Role of contractile prostaglandins and Rho-kinase in growth factor-induced airway smooth muscle contraction" ppt
... of Rho-kinase inhibition on prostaglandin-induced contraction Effects of Rho-kinase inhibition on prostaglandin-induced contraction PGE2 (A) -and PGF2α (B)-induced contraction in the absence and ... Rho-kinase U-0126, indomethacin (indo) and Y-27632 are inhibitors of MAPK, COX and Rho-kinase, respectively experiments HM participated in the design of the study and the interpretation of the ... prostaglandin; PGE2, prostaglandin E2; PGF2α, prostaglandin F2α; RTK, receptors with intrinsic tyrosine kinase activity Competing interests The author(s) declare that they have no competing interests Authors'...
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Báo cáo y học: " The role of cyclin D2 and p21/waf1 in human T-cell leukemia virus type 1 infected cells" potx
... 102 CEM mins 30 mins 45 mins 20 45 mins mins 25 30 mins 32 P Incorporation 30 15 H1 10 -5 H1 mins 30 mins 45 mins Time Figure Increased levels of cyclin E/cdk2 kinase activity in HTLV-1 infected ... with cyclin D2 and cdk4, inhibition of kinase activity was not observed, whereas addition of p16/ INK4A resulted in a strong inhibition of kinase activity In addition, the cyclin E/cdk2 kinase activity ... Figure p21/waf1 and cyclin D2 are overexpressed and in a stable kinase complex in HTLV-1 infected cells p21/waf1 and cyclin D2 are overexpressed and in a stable kinase complex in HTLV-1 infected cells...
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Báo cáo y học: "The contemporary role of blood products and components used in trauma resuscitation" pdf
... concerning in recent discussion regarding the use of rFVIIa in the setting of injury is a potential role for this material in magnifying early traumatic coagulopathy Administration of rFVIIa in supraphysiologic ... derivative of the aminoacid lysine which inhibits fibrinolysis by blocking the lysine binding sites on plasminogen Fifty-three studies including 3,836 participants have involved tranexamic acid in patients ... increase combined activity of Thrombin and Thrombomodulin Within the coagulation cascade, Thrombomodulin from endothelium complexes with Thrombin in association with activation of Protein C and...
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the role of ikkalpha, ikkbeta and nf-kappab in the progression of breast cancer
... present in most cells and normally, when in an inactive state, inhibitory proteins (IκBs) bind to NFκB dimers, masking the NLS and holding them in the cytoplasm There have been inhibitory proteins ... proteins p100 and p105, which also inhibit nuclear localisation, and therefore transcriptional activity, of NF-κB IκBα and IκBβ also contain a PEST domain, rich in proline, gutamine, serine and ... detected in some leukaemias and lymphoma This results in dysregulation of expression of genes involved in the regulation of growth and proliferation of lymphocytes such as the cytokines interleukin-2...
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Blogging and collective action the role of collective identity and social networks in engendering change
... areas in an individual’s life Informal social networks Empirical findings established the significance of interpersonal ties in influencing one’s recruitment and induction into a movement Informal ... soon apparent that blogging embarked on a new trajectory in terms of purpose and nature: that of disseminating information and facilitating exchanges of opinion, leading to unanticipated impact ... online and offline social networks must exist in order for online connections to translate into some form of collective action offline By incorporating these key elements and variables in the...
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The role of NF kb and histone deacetylase in gene regulation
... to link TRAF6 and IKK The first set of proteins described to be linking TRAF6 and IKK, are transformining growth β activated kinase (TAK1), TAK1-binding protein (TAB1) and TAK1-binding protein ... partner of IKK1 and IKK2 CUEDC2 contains CUE domains which recognize mono and polyubiquitin On top of its function in facilitating intramolecular monoubiquitination, and promote degradation of its interacting ... serine 177 and 181 within the activation loop of the IKK kinase domain (Hacker and Karin, 2006) Activated IKK2 in turn phosphorylates IκBα leading to its ubiquitination by βTrCP proteins and...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 2
... During traumatic brain injury (Sinz et al., 1999) and hemorrhagic shock (Szabo and Billiar 1999, Shinoda and Whittle, 2001), there is induction of iNOS The iNOS metabolizes L-arginine resulting ... fentanyl and 10 mg of fluanisone, and mL of midazolam (Roche, Basel, Switzerland) which contained mg of dormicum, and mL of water for injection Heparinized saline consisted of 12.5 i.u 11 heparin sodium ... production of PGs On the other hand, COX-2 is an inducible isoform that is induced by cytokines, mitogens, and endotoxins in inflammatory cells and is responsible for the elevated production of PG during...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 1
... and eosin stain of Kidneys 62 Figure 4.4.2 Hemotoxylin and eosin stain of Lungs 63 Figure 4.4.3 Hemotoxylin and eosin stain of Livers 64 Figure 4.4.4 Hemotoxylin and eosin stain of Cerebral cortex ... fluids, NOS and COX-2 inhibitors Role of angiotensin II in prolonged hemorrhagic shocked rats and therapeutic effects of conservative fluids and NOS inhibitors Role of NOS inhibitors in a combined model ... model: the role of NO and angiotensin II and the therapeutics effects of conservative fluids, NOS donor and inhibitors Paper Chapter 6: Role of NOS inhibitors in rats following combined fluid-percussion...
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báo cáo hóa học:" Preparation of TiO2 nanotube/nanoparticle composite particles and their applications in dye-sensitized solar cells" doc
... the use of TNTs in DSSCs has been explored [14-15] In the present work, the effect of combining TiO2 nanoparticles with TNTs and the resulting effect on solar cell performance have been investigated ... purely of TiO2 nanoparticles Competing interests The authors declare that they have no competing interests Authors' contributions HWC and CHL presided over and fully participated in all of the ... glass using the screen printing technique and sintered again at 450°C for 15 and at 500°C for 15 in air Assembly of the DSSCs The nanoporous TiO2 electrode films were immersed in the dye (N-719) complex...
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Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx
... conformation of inhibitor main chain Thus, different inhibitors differ not only in the binding affinity, but also in the degree of freedom of the inhibitor inside the binding tunnel Some special inhibitors ... dominating interaction between protease and inhibitor As an indicator of these hydrophobic interactions, distances between carbon atoms of inhibitor and the protease ˚ ˚ were calculated using ... Met46A and Phe153A … Met146A (A) Stacking of neighbour molecules and positions of inhibitors in subsequent protease dimers forming the helix (B) The detail of interacting residues Phenylalanines...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... binding was determined in the presence of lm unlabeled agonist and subtracted from the total binding to calculate the specific binding Internalization of [3H]BK and [3H]DAK To determine the internalization ... drop in surface binding that could not be accounted for by the amount of internalized agonist [20] As the internalization is expressed in percentage of total binding, decreasing the binding affinity ... arrangement of the amino acid residues This arrangement is probably important in Role of helix and C-termini in bradykinin receptors receptor signaling because: (a) interruption of the amphipathic...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... and difference of DCp of mutant from wild-type (%) were determined and the results for the wild-type protein and 20 mutants are given in Table The DHcal and Tm of the wild-type protein were in ... local stable segments: interactions of G75 with H121 and K9, and of G129 with K110 and K133 Other charged amino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129 ˚ (OE1 ⁄ ... fluorescent spectra of wild-type and mutants of SNase Spectra of nine proteins (WT, E73G, E75G, E101G, E122G, E129G, D77G, D83G and D95G) The spectrum of E129G (bold line) is much lower in intensity than...
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Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
... pH 8.0 and retained proteins were eluted using a gradient of NaCl prepared in the same initial buffer The presence of the recombinant Sfbgly50 was detected as above and its purity ascertained by ... length of time and determining the remaining activity in the pH optimum Fig Inactivation of the Sfbgly50 with phenylglyoxal Eect of phenylglyoxal concentration (r, mM; j, mM; m, mM; d, mM) on the inactivation ... the same increment in the pKa of E399 (Table 2) Therefore, M97 and K97 are equally effective in stabilizing the charged E399 That is unexpected, because as arginine and lysine side chains are...
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