role of oxidative stress in prostate cancer

... pathway in VSMC and the potential role of oxidative stress We also aimed at evaluating the role of protein kinase C (PKC) in the putative glucose-induced, oxidative stressmediated impairment of the ... phosphorylation induced by the NO/cGMP signalling, underlining the role of oxidative stress in this phenomenon Our working hypothesis was that high glucose increases in a short-term oxidative stress by ... endothelial and in the media layers of the aorta, indicating the occurrence of a PKC-mediated increase of oxidative stress in VSMC, the main component of the media [40] Furthermore, acetylcholine induced

Ngày tải lên: 02/11/2022, 08:46

... of E-cadherin and low expression of vimentin found in HS in comparison to ML Among the predicted features between HS and ML was the involvement of miRNA-9 in this switch Importantly, the bioinformatics ... condition of the tissues in vivo, in regards of i) architectural organization and the processes of glandular lumen formation, ii) cell-to-cell interaction, and iii) the role of cancer genes in cell ... Prediction of the involvement of EMT in HS/ML from the cell microarray data Venn diagram showing the number of genes involved in EMT in both ML and HS from the cell microarray Table (upper right) showing

Ngày tải lên: 17/06/2020, 11:40

... immunostaining was often more intense in cancers It was considered negative in 28.9%, weak in 26.5%, moderate in 39.9%, and strong in 4.7% of cancers (Table 2) High level PTPN12 staining was associated ... staining was present ‘Strong’ was assigned to all tumors showing intense, dark brown staining ‘Weak’ or ‘moderate’ was assigned to cancer showing staining intensities in between; e.g as shown in ... be relevant for prostate cancer Using PC-3 cell lines Sahu et al showed a role of PTPN12 in regulating migration of prostate cells [20] For this purpose, a preexisting prostate cancer tissue microarray

Ngày tải lên: 17/06/2020, 18:29

... probe combinations presented in Table require 3–6 FISH probes Interestingly, increasing the number of parameters from to did not appear to increase the AUC Performance of FISH with clinical parameters ... Parameter AUC DFI (Minimum) MYC Gain PTEN Homozygous MYC Gain PTEN Homozygous NMYC Gain MYC Gain PTEN Homozygous NMYC Gain MYC Gain PTEN Homozygous MYC Gain PTEN Homozygous NMYC Gain MYC Gain PTEN Homozygous ... Odds Ratio (OR) of 7.005 observed for the combination of probes (4 parameters), as shown in Table FISH parameters were independent of clinical parameters in the model Clinical information was

Ngày tải lên: 23/07/2020, 23:51

... involved in cancer cell growth and survival regulation [3, 4] MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, ... essential role in the activation of ROS/ Akt signaling and cell invasive phenotype and identify a novel link between RAB35 and MICAL1 in regulating breast cancer cell invasion These findings may ... determined by immunoblotting assays (Fig 1a) Enhanced motility of breast cancer cells is a critical step in promoting tumor metastasis, but roles of MICAL1 in breast cancer motility remain to

Ngày tải lên: 20/09/2020, 14:58

... prostatic intraepithelial neoplasia (HGPIN, red box) and negative staining in prostate cancer (green box) (f) Heterogeneous ERG immunostaining in HGPIN (green asterisk) Red asterisk indicates invasive ... appearing prostate epithelium (a and c) with corresponding H&E staining (b and d) The blue arrow indicates normal prostate epithelium, the green arrow indicates cancer cells (e) Positive staining in ... This finding was obviously caused by the high rate of interfocal heterogeneity in multifocal cancers More than 60 % of our patients had more than one cancer focus in their prostates including 35

Ngày tải lên: 20/09/2020, 15:24

... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 1976;72:248–54 13 Ang J, Fang BL, Ashman LK, Frauman AG The migration and invasion of human prostate ... AR at serine 210 (pAR(210)) [41, 43] Although the role of circulating oestrogen in PCa carcinogenesis is still controversial [44], it may be worth investigating the role of ER and PGR in CRPC ... 11 of 14 more is known about the role of NR2F2 in PCa [45, 49], both COUP-TF genes, NR2F1 and NR2F2 display nearly 100 % homology in their DNA-binding domain (DBD) and ligand- binding domain

Ngày tải lên: 22/09/2020, 23:34

... suppresses migration and invasion in prostate cancer cells Integrin β8 has been implicated in tumor cell invasiveness [11] To analyse functional effects of integrin β8 in prostate cancer cells, we employed ... regulates integrin β8 expression and that integrin β8 plays a hitherto unrecognized role in the motility of prostate cancer cells and thus targeting integrin β8 may be a new treatment strategy for prostate ... influence integrin clustering and in some cases, inhibit integrin downstream signaling [19,20] Furthermore, ephrin-A1 and EphA2 have both been shown to co-localize with integrin α3 [20-22] In breast cancer

Ngày tải lên: 30/09/2020, 11:10

... expression of MMP-1 contributes to the brain colonizing potential of human breast cancer cells in xenograft models of cancer progression Members of the MMP family play important roles in normal ... activation of STAT3, which has been linked to elevated expression of various genes, including VEGF, cyclin D and survivin [27] Transcriptional regulators of MMP-1 in cancer cells include STAT3 ... outcome and often produced adverse events, including dose-limiting joint toxicity [52,53] As more details of functions of MMP-1 in metastasis to the brain and other organs are defined, this information

Ngày tải lên: 05/11/2020, 08:01

... overview of involving in main knowledge of chapter two The concepts of sentence stress are identified clearly according to many points of view Some functions of sentence stress are also found in this ... sentence stress while speaking Some of them about 12.5% often use sentence stress in communication, representation, and reading 25% of them rarely use sentence stress in English speaking competence ... of sentence stress Particularly, the role of sentence stress is emphasized and mainly focused in English intonation patterns Sentence stress is another golden key for speaking and understanding

Ngày tải lên: 08/04/2021, 08:15

... overview of involving in main knowledge of chapter two The concepts of sentence stress are identified clearly according to many points of view Some functions of sentence stress are also found in this ... sentence stress while speaking Some of them about 12.5% often use sentence stress in communication, representation, and reading 25% of them rarely use sentence stress in English speaking competence ... of sentence stress Particularly, the role of sentence stress is emphasized and mainly focused in English intonation patterns Sentence stress is another golden key for speaking and understanding

Ngày tải lên: 05/08/2021, 21:24

... Anaplerotic fixation of CO 2 by PEPC increased in re- sponse to O 3 in both groups of seedlings (Figs. 5E–5H) dur- ing the growing season. The level of activity decreased in the neoformed flush of the episodic ... is one of the enzymes of this cycle. In the present study, GR activity increased two-fold in sugar maple seedlings in re- sponse to increasing O 3 stress. This enzyme ensures a high level of GSH ... preformed flush, consisting of preformed leaves and the second flush as neoformed flush consisting of neoformed leaves as described by Gregory [18]. 2.1.2. Harvesting of seedlings Seedlings were randomly

Ngày tải lên: 08/08/2014, 00:22

... cytotoxicity of the PI3K inhibitor LY294002 Moreover, the efficacy of combined treatment with Akt inhibitors and ionizing radiation in prostate cancer cells was examined Materials and methods: Prostate cancer ... down-regulation of p-Akt may be required for the anti-neoplastic action of small molecule inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate ... involve apoptosis induction implicating a role of proliferation inhibition or the induction of non-apoptotic or delayed cell death modes Impact of ErPC3 and ionizing radiation on the levels of

Ngày tải lên: 09/08/2014, 09:20

... Background Quality of life is becoming one of the most significant issues in treatment decision-making, in general, and more so in prostate cancer [1] Thus late rectal and urinary damage became ... toxicity observed in patients during their last inspection are shown In the cohort of patients included in the WP group, pretreatment GI toxicity of grade was found in the history of patients (1.72%), ... recognition of prostate cancer cells Thus we can hypothesize, according to results of Hurwitz et al [12] that the enhanced immune function, involving resting and terminally differentiated NK cells during

Ngày tải lên: 09/08/2014, 09:20

... several protein tyrosine- and histidine kinases [810], and inhibits topoisomerase I and II [11] These effects result in alterations of several intracellular and extracellular pathways including cell ... (figure 1) In contrast, in our passages of PC-3 cells we could not stain any of these receptors Genistein inhibits clonogenic cell survival in LNCaP and PC-3 In PC-3 cells we tested genistein concentrations ... line PC-3 has been demonstrated in vitro and in vivo [26,27] Our study analyzes the interactions of irradiation and genistein or estradiol incubation in androgen sensitive LNCaP and androgen independant

Ngày tải lên: 09/08/2014, 09:22

... for 30 min. A mixture of plasma samples and 2.5 × 10 4 infectious units of GFP-ca rrying XMRV were incubated at 37°C for 30 min before infecting 293T cells (5 × 10 4 ). Three days post-infection, ... RNASEL R462Q in our clinical samples (Table 5). Phylogenetic analyses of MLV-like sequences in prostate tissue DNA XMRV has been PCR amplified from prostate cancer samples in a number of studies ... (IAPs). Together with our data, these findi ngs highlight the difficulty of avoiding DNA contamination in clinical samples and the risk of testing contaminated samples as XMRV-posi- tive by sensitive

Ngày tải lên: 13/08/2014, 01:20

... to this kind of stress in referring, proclaiming, and reporting utterances Tonic stress is almost always found in a content word in utterance final position Consider the following, in which the ... clear to you The role of sentence stress in enhancing English speaking competence Sentence stress is the governing stress in connected speech All words have their individual stress in isolation When ... 34 8.1 Final intonation 36 8.1.1 Rising-falling intonation 36 8.1.2 Rising intonation 37 8.2 Non-final intonation 39 8.2.1 Rising-falling intonation...

Ngày tải lên: 17/03/2014, 16:12

... use of vitamin E and vitamin C in combination reduced the prevalence and incidence of AD in an elderly population; however, the use of either of these vitamins alone had no effect [110] Finally, ... gp91phox and an increase in p47phox protein expression in monocytes primed with INFγ [59] Figure Activation of the Rac GTPase Activation of the Rac GTPase GTP-binding of the Rac protein confers an ... src-family tyrosine kinases or inhibition of phosphatidylinositol-3 kinase (PI3K) attenuates ROS production These findings suggest that these kinases are involved in upstream signaling cascades responsible...

Ngày tải lên: 19/06/2014, 22:20

... Conclusion This study provides insight into the involvement of oxidative stress in the pathogenesis of OA from the viewpoint of oxidative stress induced genomic instability, especially telomere ... Our findings clearly show the presence of oxidative stress in degenerating cartilage, and the resultant telomere erosion and dysfunction of chondrocytes in vitro and in vivo, suggesting a role ... and maintenance of GAG content in articular cartilage in tissue culture Interestingly, treatment of cultured cartilage with an antioxidative agent not only inhibited GAG loss but also maintained...

Ngày tải lên: 09/08/2014, 06:22

... domain REC domains play a crucial role in bacterial signalling systems They may also be involved in protein-protein interactions (Galperin 2010) Phosphorylation of the REC domain may be involved ... and binds phosphatidic acid (PA) via a TAPAS domain in its unique N-terminal region An increase in intracellular CA2+ brings about binding of PDE4A1 to PA and insertion into lipid bilayers in an ... Signalling in the Prostate and Prostate Cancer 105 1.10.1 cAMP PDEs in Prostate Cancer 105 1.10.2 Protein Kinase A and Prostate Cancer 106 1.10.3 EPAC and Prostate Cancer 108...

Ngày tải lên: 22/12/2014, 21:15