role of microbial glycosylation in host cell invasion

... misfolding of this protein, resulting in its partial retention in the ER and its rapid digestion thereafter [16,19] Accordingly, our results indicate that the intracellular trafficking of N -glycosylation ... transporter in HeLa cells was abolished by tunicamycin, a potent inhibitor of N -glycosylation [18] We found that inhibition of N -glycosylation processing by dMM did not 1633 Role of N -glycosylation ... terminal structures of the N-glycans of GAT1, N -glycosylation processing of NNN was inhibited by 1-deoxymannojirimycin (dMM) Inhibition by dMM leads to the formation of NNN molecules containing...

Ngày tải lên: 19/02/2014, 17:20

... IL-10 Interleukin-10 IL-1β Interleukin-1 beta IL-12 Interleukin-12 IL-13 Interleukin-13 xxii IL-21 Interleukin-21 IL-22 Interleukin-22 IL-23 Interleukin-23 IFN-γ Interferon gamma ITK IL-2 inducible ... protein downstream of Shc GRB2 Growth factor receptor-bound protein IL-2 Interleukin-2 IL-2Rα Interleukin-2 receptor alpha IL-4 Interleukin-4 IL-5 Interleukin-5 IL-6 Interleukin-6 IL-8 Interleukin-8 ... are in an inactive state due to the thiol (SH-) group of the cysteine residue within the pro-domain interacting with the Zn2+ ion in the catalytic domain Disruption of this cysteine-zinc interaction,...

Ngày tải lên: 24/08/2014, 11:37

... important role in mediating signaling cascades Reports pertaining to the studies of Dok-1 and were mainly explored in cells of hematopoietic origin for the following reasons Hematopoietic cells, in ... studying the role of Dok-3 in BCR signaling using either A20 mouse B lymphoma cell line or the DT40 chicken B cell line Although these studies indicate a role for Dok-3 in negative signaling of ... control of B cell signaling 1.4.1 FcγRIIB receptor FcγRIIB receptors are single-chain molecules bearing IgG-binding sites in their extracellular domains and cytoplasmic domains containing an immunoreceptor...

Ngày tải lên: 14/09/2015, 09:08

... SH3 domain binding protein Geneticin Gancyclovir Growth factor receptor-bound protein Haemagglutinin 1,3,4,5-tetrakisphosphates Immunoglobulin Interleukin Inositol 3,4,5-triphosphate Insulin receptor ... chromosome 10 Protein tyrosine kinase Ras GTPase activating protein Src-homology Src-homology SH2-containing inositol 5’-phosphatase-1 SH2-domain containing protein tyrosine phosphatase-1 Son of sevenless ... Downstream of tyrosine kinases Extracellular-signal-regulated kinase Embryonic stem Florescence activated cell sorting Fluorescein isothiocyanate Interleukin Four Receptor Interacting Protein RasGAP...

Ngày tải lên: 14/09/2015, 10:44

... The role of FGFR3 mutation in tumour initiation, progression and invasion of urothelial cell carcinoma in mice Mona Foth Submitted in fulfilment of the requirements for the Degree of PhD Beatson ... carcinoma (Ta), breakthrough of basement membrane with tumour cells in connective tissue (T1), invasion into muscle (T2), invasion into fat layer (T3) and invasion of neighbouring tissues (T4) Source: ... expression of secreted protein Shh in a subpopulation of CK5-positive basal cells, which are capable of regenerating all cell types within the urothelium (Shin et al., 2011) CK5-expressing basal cells...

Ngày tải lên: 22/12/2014, 20:56

... 3821 Role of CerK in PPARb-induced keratinocyte survival K Tsuji et al Fig Schematic model illustrating the role of CerK in cell survival mediated by PPARb in mouse keratinocytes Under injury ... keratinocytes [14] 3816 In this study, we examined the interaction between CerK and PPARb, and its role in regulating keratinocyte survival We report that in a mouse keratinocyte cell line upregulation ... upregulation of CERK expression by activation of PPARb results in a decrease in intracellular Cer levels and enhancement of cell survival Results Skin barrier disruption in hairless mice induces mRNA...

Ngày tải lên: 18/02/2014, 18:20

... SCC cells irrespective of the p53 status should be clarified In the present study, we examined the effects of BNCT on the proliferation, cell cycle, and cell cycle-related proteins of oral SCC cells ... cells showing wild- or mutant-type p53 with the same background and indicated the role of p53 in the suppressive effect of BNCT Methods Cells The oral SCC cell line SAS showed the phenotype of wildtype ... iodide in PBS, cells were analyzed with a fluorescence-activated cell sorter (FACSort; Becton Dickinson, Mountain View, CA) The percentage of cells at different phases of the cell cycle was determined...

Ngày tải lên: 09/08/2014, 10:20

... suggesting that the development of ACS in SCD may be accompanied by PMN infiltration into the lungs [31] SCD patients have increased levels of the PMN chemokine IL-8 during VOC, suggesting that during ... lipids, resulting in changes in fluidity of the bilayer Additionally, there is increased ion permeability, inactivation of membrane enzymes and receptors, and covalent cross-linking of lipid Once ... clinical variability in disease course Further work is necessary to define more clearly the oxidant/antioxidant profile of SCD patients at baseline and during VOCs, including ACS, and to examine...

Ngày tải lên: 12/08/2014, 18:20

... proteins; calerticulin and calnexin Calerticulin is a calcium-binding protein that participates in calcium signaling and linked to activation of the transcription factor nuclear factor of activated ... activation of such genes in HTLV-1 infected T-cells independently of specific external signals might be a trigger for initial steps in the oncogenic transformation of HTLV-1 infected T-cells in culture ... amino acid identity and a similar domain structure that includes amino-terminal kinase domain, a dimerization leucine zipper domain, and helix-loop-helix motifs, which are involved in regulating...

Ngày tải lên: 13/08/2014, 13:20

Ngày tải lên: 13/11/2014, 10:36

... CD4+ T cell responses to influenza in the absence of IFN-γ signaling119 4.7 Role of IFN-γ in controlling lung damage after an influenza infection121 4.8 IFN-γ influences the contraction phase of ... Rates of proliferation of CD8+ T cells in the absence of IFN-γ signaling 140 5.4 Interferon gamma and cell death 146   viii 5.4.1   Rate of survival of CD8+ T cells in the absence of ... with an increased fraction of influenza-specific CD8+ T cells expressing the interleukin-7 receptor at the peak of the response, resulting in enhanced numbers of memory precursor cells in IFN-γ-/-...

Ngày tải lên: 10/09/2015, 09:25

... Immunohistochemistry IκB Inhibitor κB IL-1β Interleukin-1 beta IL-4 Interleukin-4 IL-5 Interleukin-5 IL-6 Interleukin-6 IL-8 Interleukin-8 IL-12 Interleukin-12 IL-13 Interleukin-13 IP-10 Inducible protein-10 JNK ... activation of inhibitor of κ kinases (IκK) by other kinases is described Downstream signaling of receptor tyrosine kinases is shown using the example of tyrosine kinase receptor A (TRKA) and illustrating ... vital in promoting clonogenic growth of HRS cells Recombinant neutralizing antibody of CCL5 can inhibit the basal proliferation of these HL-derived cell lines (Aldinucci et al., 2008) B cells of...

Ngày tải lên: 10/09/2015, 09:29

... area of great interest, as loss of genomic integrity is often implicated in tumorigenic and oncogeneic events Critical to the ability of healthy cells in maintaining genomic integrity are the cell ... Induction in Hela cells in response to Adriamycin and varying doses of p38 inhibitor Figure 5.7: Inhibition of p38 induces apoptosis in A549 cells Figure 5.8: Apoptosis induction by MMS and LY479754 in ... implicated in cell migration, invasion, proliferation, angiogenesis, cell differentiation and cell survival (5) MAPKs are serine/threonine protein kinases mediating the response of cells to extracellular...

Ngày tải lên: 11/09/2015, 09:02

... chromatin resulting in either gene activation or repression For instance, the appearance of histone acetyltransferases, including cAMP-responsive element binding (CREB) protein-binding protein (CBP), ... 2000) CDK protein levels remain stable during the cell cycle, in sharp contrast to their activating proteins, the cyclins The oscillatory expression of most cyclin proteins during cell cycle progression ... the cyclin-dependent kinases (CDKs), a family of serine/threonine protein kinases that are activated at specific points of the cell cycle To date, nine CDKs have been identified and, of these,...

Ngày tải lên: 13/09/2015, 20:18

... production in breast cancer cell lines Over-expressing PrP in MCF7 breast cell line Over-expressing PrP in transfected MCF7 cells reduces cell proliferation rate Over-expressing PrP in transfected ... Overexpression of PrP in human embryonic kidney 293 cell lines, rabbit epithelial Rov9 cell lines, and murine cortical TSM1 cell line resulted in cells sensitive to the apoptotic inducer, stauroporine, ... higher in normal breast cell line (MCF10A) than breast cancer cell lines (MCF7, SK-BR-3, and MDA-MB-231) Proliferation rate in breast cancer cell lines Up-regulation of p53 in breast cancer cell lines...

Ngày tải lên: 12/10/2015, 17:36

... Summaries of experimental procedures 54 2.29.1 Influenza A infection of RAW264.7 murine macrophage cell line in vitro 54 2.29.2 An investigation of the role of caspase-1 in an in vivo ... Expression of caspase-1 and growth of virus in influenza A/PR/8/34 (H1N1) infection of RAW264.7 macrophages 130 4.3 Insights into the role of caspase-1 using a murine model of influenza ... embryonic stem cells FBS foetal bovine serum g gram h hours xv H&E haematoxylin and eosin stain HRP horseradish peroxidase ICE interleukin-1β-converting enzyme IFN interferon IL interleukin INF infected...

Ngày tải lên: 13/10/2015, 16:41

... ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma derived cell ... expression of the ankyrin-repeat oncoprotein (gankyrin) in human hepatocellular carcinoma Gankyrin binds to the cell- cycle regulator CDK4 and the S6b ATPase subunit of the regulatory component of the...

Ngày tải lên: 31/10/2012, 15:28

... proteins indeed changes the expression of apoptosis profile of host cells, which probably results in the carcinogenesis of liver cells Materials and methods Cell lines and isolation of RNA HepG2 cell ... important regulators of cell apoptosis, it is possible that HBV infection might alter the cIAPs gene expressing of liver cell, finally instigating the carcinogenesis of the cells it infected This is ... of cIAP2 was obviously higher in the HepG2.215, a cell line expressing HBV, than in the HepG2, a cell line absence of HBV (Figure 1A, 1&2) The up-regulation of cIAP2 expression in HepG2.215 cells...

Ngày tải lên: 02/11/2012, 11:17

... viewed as limited in raising the profile of women in management However, during this period of research, there was an emerging recognition of the importance of a concern for people in the behavioural ... more typical of male behaviours Of interest is the inclusion of ‘‘control’’ as a significant feature of the transactional style, common in definition of leadership in the 1990s In contrast, women ... superiority in utilising effective leadership styles of interaction with their subordinates and organisational outcomes, actually translates into a dramatic increase in the numbers of women being appointed...

Ngày tải lên: 08/10/2013, 15:56

... The role of FDI in institution building Over the past 20 years, foreign financial institutions, mainly foreign banks have played an important role in the opening up of Chinese financial industry ... technology, in exploring on-line banking business as well as in promoting non-bank financial institutions Efforts should be made in upgrading financial operations and financial services, and in conducting ... China Statistics Yearbook, China Financial Yearbook and People’s Bank of China Statistics Quarterly bulletin B Development of direct financing and indirect financing markets providing financing...

Ngày tải lên: 18/10/2013, 07:15