role of dna repair and cell cycle control genes
The role of p38 MAPK in cell cycle checkpoint control following DNA damage
Ngày tải lên :
11/09/2015, 09:02
... induction of the mammalian cell cycle (376) The cell cycle is the process which cells use to undergo celldivision, where one cell divides into two identical daughter cells Regulation of the cell cycle ... establishment and maintenance of a DNA damage-induced cell cycle arrest in G2 The ability of cancer cells to establish a cell cycle arrest in response to genotoxic agents is one of the reasons ... necessary to control the rate of proliferation as well as the accuracy of duplication 1.6.1 The Mammalian Cell Cycle The mammalian cell cycle consists of a set of tightly regulated and ordered...
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Elucidation of the physiologic role of TRIP br2 in cell cycle regulation and cancer pathogenesis
Ngày tải lên :
13/09/2015, 20:18
... schema of cell cycle progression: simplifying complexity 25 1.1.2 The control of cell cycle progression 27 1.1.2.1 RB/E2F/DP-mediated transcriptional regulation of cell cycle ... paradigm of the cell cycle and cancer 1.1.1 The general schema of cell cycle progression: simplifying complexity Cell division is characterized mainly by two consecutive processes, DNA replication and ... The control of cell cycle progression 1.1.2.1 RB/E2F/DP-mediated transcriptional regulation of cell cycle progression The E2F family of transcription factors integrates cellular signals and coordinates...
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Senescence and Cell Cycle Control
Ngày tải lên :
25/10/2013, 21:20
... tumor-suppressor pathways and cell cycle control during cellular senescence Role of the p53 Pathway in Senescence The tumor-suppressor p53 plays a key role in induction of senescence as evidenced ... Studies using acute disruption of p21Cip1 by conditional gene targeting will be informative for better understanding of the role of p21Cip1 in senescence of mouse cells Role of the Rb Pathway in Senescence ... 2004) Thus, Bmi-1 plays a role in the timing of senescence Senescence and Cell Cycle Control 263 induction by controlling p16INK4a (and p19ARF in MEFs), while the detail of the promoter regulation...
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ELUCIDATING THE ROLE OF REDOX EFFECTS AND THE KU80 C-TERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU
Ngày tải lên :
24/08/2014, 11:02
... THE ROLE OF REDOX EFFECTS AND THE KU80 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks (DSB) are among the most lethal forms of DNA damage and can ... via stimulation of a conformational change when oxidized resulting in a decrease in DNA binding activity To further understand these methods of regulation of Ku and DNA- PK, a pair of mutants has ... Purity of Ku80CTR 26 DNA binding activity is not affected by truncation or the addition of Ku80CTR 27 The effects of oxidation on DNA binding of wtKu and Ku70/80C 29 Effects of...
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The role of TRIP br proteins in the regulation of mammalian gene transcription and cell cycle progression
Ngày tải lên :
16/09/2015, 17:12
... Tissue culture cell lines 36 2.1 Maintenance and Handling of Tissue Culture Cells 37 2.2 Preparation of Competent Bacterial Cells 37 2.3 Transformation and Maintenance of Plasmid DNA 38 Methods ... Inhibition of cell proliferation by decoy peptides 74 Cell cycle analysis of U2OS cells after decoy peptide 76 treatment xi FIGURE 17: Protein expression analysis of cell cycle regulators in 79 U2OS cells ... family and a subunit encoded by the DP family of genes 43 In mammalian cells, there are six E2F genes (encoding E2F1, E2F2, E2F3, E2F4, E2F5 and E2F6) and two DP genes (encoding DP1 and DP2) E2F and...
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Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor
Ngày tải lên :
25/10/2013, 21:20
... G1 and A -and B-type cyclins in G2/M) that control a family of Cdks (cyclindependent kinases) greatly enhanced our understanding of the complexity underlying control of the cell cycle Control of ... 1997; Zachariae and Nasmyth 1999) These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested ... number of mutants engineered in genes encoding components of the cell cycle machinery and the pRB and p53 tumor suppressor pathways The universality of the cell cycle in all eukaryotes strongly...
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cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
Ngày tải lên :
08/04/2014, 12:50
... and Fission Yeasts Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts Tim Humphrey and Amanda Pearce Summary The cell cycles of the budding yeast Saccharomyces cerevisiae and ... Genetic control of cell size at cell division in yeast Nature 256, 547–551 13 14 Nurse, P and Thuriaux, P (1977) Controls over the timing of DNA replication during the 14 cell cycle of fission ... (Swi4/Swi6 cell cycle box) elements and comprises Swi4 and Swi6 MBF (MCB binding factor) recognises MCB (MluI -cell cycle box) elements and is composed of Mbp1 and Swi6 MBF binding is cell cycle- regulated...
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aging of the genome the dual role of dna in life and death mar 2007
Ngày tải lên :
11/06/2014, 10:21
... theory of evolution by natural selection and Watson and Crick’s discovery of the molecular structure of DNA The grand understanding of the logic of life would prove equally important for understanding ... human white blood cells and in the sperm of trout However, the nature of the genetic code and a mechanism for how DNA was able to transfer this information from cell to cell and how it could convert ... phosphorylation and the synthesis of ATP Critically important to all biology and shaping our understanding of the fundamental mechanisms of this most important of all cellular activities, the elegance of...
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Báo cáo hóa học: " The role of feed-forward and feedback processes for closed-loop prosthesis control" doc
Ngày tải lên :
19/06/2014, 08:20
... (PC) to control the vibrotactile stimulation The hardware allows us to control the pulse width and period of stimulation This enabled independent control of the duty cycle and frequency of pulses ... random unpredictable delays to the hand controller In contrast to experiments and 2, where the control of the hand was repeatable and predictable, this experiment was designed to examine the role ... of the hand to enable differential force control Differential Force Control We used a ‘gated ramp controller, for two-channel differential position and force control (e.g see [24]) Subjects controlled...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
Ngày tải lên :
20/06/2014, 04:20
... proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated with proliferation and angiogenesis ... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of ... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
Ngày tải lên :
20/06/2014, 07:20
... proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated with proliferation and angiogenesis ... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of ... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
Ngày tải lên :
09/08/2014, 09:20
... component of total DNA breakage, and that the inhibition of apoptosis allows for more proper estimation of radiation-induced DNA DSB repair and apoptosis itself First, we found spontaneous cleavage of ... stage of two independent processes DNA repair and anoikis–at each time point This type of apoptosis is physiologically induced to prevent proliferation of cells at inappropriate locations Tumour cells ... intensity of the DNA was acquired, and transformed to arbitrary units of optical density using a digital imaging analysis system (GelDoc 2000 and Quantity One software, Bio-Rad) The sum of fluorescence...
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Báo cáo y học: "Two specific drugs, BMS-345541 and purvalanol A induce apoptosis of HTLV-1 infected cells through inhibition of the NF-kappaB and cell cycle pathways" ppsx
Ngày tải lên :
10/08/2014, 05:21
... T -cell leukemia virus type Tax and cell cycle progression: role of cyclin D-cdk and p110Rb Mol Cell Biol 1998, 18:3620-3632 Akagi T, Ono H, Shimotohno K: Expression of cell- cycle regulatory genes ... Cell cycle analysis of cells treated with or without drugs Cell cycle analysis of cells treated with or without drugs For fluorescence-activated cell sorting (FACS) analysis, both untreated and ... expression of some cell cycle- associated genes and accelerate cell cycle progression in G1 phase [46-50] Tax targets cell cycle regulators such as p53, cyclin dependent kinases (CDKs) and 6, cyclin...
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Báo cáo y học: " Differences in allergen-induced T cell activation between allergic asthma and rhinitis: Role of CD28, ICOS and CTLA-4" potx
Ngày tải lên :
12/08/2014, 13:22
... survival Ligation of CD28 promotes the production of IL-4 and IL-5 and provides resistance to apoptosis and long-term expansion of T-cells As CD28, ICOS is a positive regulator of T cell activation ... (Antony, France) None of the allergens contained detectable amounts of LPS Specific stimulation of T cells Optimal dose of stimulatory pDerp1 and kinetics of T cell cytokine secretion and proliferation ... IL-4+ and IL-13+ cells were considered as Th2 cells, IFN-g + cells as Th1 cells IL-10+ cells were considered as belonging to Treg cell population Co-receptor study To determine the role of co-receptors...
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Báo cáo y học: " The role of cyclin D2 and p21/waf1 in human T-cell leukemia virus type 1 infected cells" potx
Ngày tải lên :
13/08/2014, 13:20
... CDK4 CycD2 NS Figure Cell cycle analysis of cyclin D2 and p21/waf1 Cell cycle analysis of cyclin D2 and p21/waf1 (A) ChIP assays were performed using G0 cells (0 hour) and G1/S cells (6 hour) as ... Cancer cell cycles Science 1996, 274:1672-1677 Ewen ME: Where the cell cycle and histones meet Genes Dev 2000, 14:2265-2270 Akagi T, Ono H, Shimotohno K: Expression of cell- cycle regulatory genes ... p21/waf1 and cyclin D2 in C81 cells at hours The lack of cdk4 in p21/waf1/cyclin D2 complexes in C81 cells at hours and CEM cells at and hours may explain the observed loss of activity Effect of purified...
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Cell cycle control and fate determination during male gametogenesis in arabidopsis thaliana
Ngày tải lên :
12/09/2015, 09:28
... factors, and WEE kinases to control the progression through cell cycle phases (Stals and Inze, 2001) Genome-wide analysis of core cell cycle genes in Arabidopsis showed plant specific components of cell ... mother cell divides symmetrically with differentiation into two neurons INTRODUCTION 1.1.3 Coordination of cell cycle and cell differentiation In a developmental context, the cell cycle and cell ... further control cell- cycle progression, it is hard to pinpoint when and where the initial defect appears and what is the nature of the defect (derepression of cell proliferation or loss of cell...
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Wip1 regulates proliferation of adult neural progenitors through p53 dependent g2 cell cycle control
Ngày tải lên :
14/09/2015, 08:43
... Wip1 in tumorigenesis 15 iii 1.3.4 Wip1 in DNA repair and cell cycle regulation 17 1.3.5 Possible role of Wip1 in neurogenesis 18 1.4 Wip1 related cell cycle regulators in adult neurogenesis 21 ... the general role of Wip1 as an oncogene 1.3.4 Wip1 in DNA repair and cell cycle regulation In terms of cellular processes, study of Wip1 has been focused mainly on its interaction with DNA damage ... length of cell cycle of NPCs in SVZ is 14 hours (Smith and Luskin 1998), almost all the proliferating SVZ cells should have been labeled during the 12-hour tandem injection and the labeled cells...
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Exploration of the functional significance of mig 2 in human cancer cell susceptibility to cytotoxic agents and cell growth control a pilot study
Ngày tải lên :
05/10/2015, 22:15
... list of cell cycle control provided by GeneServer, which is an integrated database of genes, gene products and their involvement in diseases Some of the cell cycle control genes in the GeneServer ... control 1.2.2.1 Overview of cell cycle and growth control The mammalian cell cycle is the focus of a large number of studies at both genetic and molecular level The cell cycle can be described ... mig-2 1.2.2 11 Possible role of mig-2 in cell growth control 1.2.2.1 Overview of cell cycle and growth control 11 ii 1.2.2.2 Mig-2 involved in mitogenic signaling cell cycle control 12 1.3 Recent...
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Involvement of nm23 m2 in dopaminergic neuronal differentiation and cell cycle arrest
Ngày tải lên :
08/11/2015, 17:09
... changes of MN9D cells 80 after 24hr post-transfection of control siRNA and nm23-M2 siRNA Figure 3.21 A graph showing the percentage of MN9D cells after 48hr post- 81 transfection of control and nm23-M2 ... overexpression of nm23-M2 itself in MN9D cell line resulted in significant increase in the number of cells bearing neurites and an alteration of the cell cycle, increased G1phase Analysis of immunoblots ... gene and controls the cell cycle, S-phase, indirectly via a cellular cofactor in the murine cell line (Lee et al 1997) and nm23-M2 activates endogenous c-myc expression both at transcriptional and...
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