... growth from the second quarter of 2000 into jobclasses using beginning size of firm, mean size of firm over the period,and end size of firm. If the beginning size of the firm is used to classifyfirms, ... longitudinal data from individualfirms.4The job figures presented in Chart 1 classify firms into size classes based ontheir size at the beginning of the period, which favors a finding of higher ... rate in the prerecession period from 1997 to2000. Recessions often find individuals moving out of traditional employment andinto self-employment, which explains some of the discrepancy in growth...
... diseaseprevention, e.g., lycopene in tomatoes and sulfurofane in broccoli for reduc-ing cancer risk, lutein in vegetables for reducing risk of macular degenera-tion, etc. The advancing Þelds of human and plant ... Advantages of insect- and virus-resistant crops include improvedyields and reduced use of pesticides. An additional beneÞt of Bt corn isreduced contamination by fumonisin-producing fungi. Fumonisin ... consumed in place of saturated fattyacids. It is possible to increase the content of vitamin E, a natural antioxidant,and to insert the capability of producing plant-based omega-3 fatty acidsinto...
... complexes of cyclin-dependent kinases (cdks)and their corresponding cyclins. A prominent example of such a pair is cyclin B and cyclin-dependentkinase 1 (cdk1 ⁄ Cdc2) controlling the checkpointbetween ... Dimethyl sulfate footprint-ing of the CDE in the presence of SV40 large T indi-cated a loss of protein occupation on this site in vivo[79].Elevated expression of cyclin A, cyclin B, Cdc25Cand Cdc2 ... repression of genes, such as cyclinA and cyclin B1, in dividing cells [92]. Furthermore,depletion of Lin-9 and B-Myb in p53) ⁄ )MEFs causedlowered expression of cyclin A, cyclin B and Cdc2 [94].In...
... role for GTP activation of CTP synthesis. A mechanism in which GTP only acts to increase the rate of hydrolysis of glutamine has some flaws in explaining the inhibition of DON-labeledenzymewhenassayedwithNH4Cl ... the binding of GTP to the allosteric site promotes coordination of thephosphorylation of UTP and hydrolysis of glutamine foroptimal efficiency in CTP synthesis rather than just acting toincrease ... of the enzyme will notinterfere with the interpretation of the data. In this research, we have analyzed the steady statekinetics of the glutaminase reaction of CTP synthase fromL. lactis in...
... tyrosinase 320 amino acidsCore domain 36–357Copper binding motifArg40Cys84Tyr349Tyr347Copper binding motifTrypsinisedpro-tyrosinase 332 amino acidsCore domain 36–370Copper binding ... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase geneand surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the ... kDa) assuming the removal of theN-terminal methionine.Reconstitution of recombinat V. spinosumtyrosinase with copperThe holo-forms of tyrosinase were obtained after puri-fication by adding copper...
... value determined from the amino acidsequence (45 320 Da).Measurement of the Kd of PLP bindingequilibriumPLP binding equilibria were analyzed taking advantage of the protein intrinsic fluorescence ... arelocated in the cores of the large and small domains,respectively, and appear to stabilize their protein scaf-folds, allowing the proper positioning of the residuesinvolved in PLP binding, substrate ... binding propertiesThe affinity of wild-type and mutant forms for thecofactor was measured to evaluate the impact of themutations on the structure of the PLP binding site.Because PLP binding...
... sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinasepotentiates insulin action in muscle of insulin-resistant rats[39], and that prolonged GSK3 inhibition ... inhibition improves insulin responsiveness in muscle of insulin resistant animals it had no insulinpotentiating effect in skeletal muscle of lean animals [39,41].To assess whether chronic inhibition ... effective ÔchannellingÕ of glucose intoglycogen in response to insulin. However, one of thepotential difficulties in interpreting data from studies thatutilize Li as an inhibitor of GSK3 is that...
... area of an image for speech information. Following a speech-reading experiment using eye-tracking equipment with nativespeakers of English, Lansing and McConkie (1999) suggestedthat in terms of ... minimal reference to the outline duringrecording. The first part of the lecture covered definitions of terms and a brief history of ceramics, which tended to be done in narrative form. Most of ... in alarge room or hall to a large group of people, pointing out that in suchcircumstances visual cues would be of little to no value. This is a veryspecific interpretation of the meaning of...
... thepurification of an AnfA variant reported by Austinet al. [22]. In their study, the N-terminal domain of AnfA was truncated to prevent the intrinsic aggrega-tion of the intact form during purification. ... of theN-terminal domain in this variant. Because sensing is aprincipal function of regulatory proteins, the isolation of VnfA and AnfA with their sensor (GAF) domainsis highly desirable. In ... effect of the nucleotide binding was reported in a study of the limited trypsin digestion with NifA + MgADP, in which binding MgADP to the central AAA+ domainis ascribed to the trigger of a conformational...
... for disbursing of loans and collecting loan repayments. Another promising track for savings banks is to invest their savings deposits in microfinance institutions by specialising in wholesale ... wholesale lending to microfinance institutions for lending to their micro borrowers. Savings banks could refinance well-functioning microfinance institutions individually or indirectly through ... sprung out of the savings banks movement, have done a Bringing the Hidden Giants to the Footlight: the Roleof Savings and Retail Banks in Increasing the Level of Access to Financial ServicesChris...
... modified serine [22].PfACP is a protein of 137 residues, inclusive of signal and transit sequences, required for targeting of the protein to the apicoplast. The mature protein com-prises 79 amino acids ... availableonline:Doc. S1. Crosslinking using glutaraldehyde.Fig. S1. Oligomeric status of PfACP determined byglutaraldehyde crosslinking.This material is available as part of the online articlefrom ... thrombin cleavage site was engineered in the pET28a vector immediately after the N-terminal His-tag.The N-terminus His-tag of ACP was cleaved by the addition of 1 U of thrombin from bovine plasma...
... C6position of glycogen in the ADM group is indicative of activated 6-phosphofructo-1-kinase, which can lead toincreased13C labeling at the triose level. Such a mechanismcan lead to increases in labeling ... Regulation of hepatic carbohydrate metabolism in mouse liver (Eur. J. Biochem. 269) 4425Elucidation of the roleof fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice using in vivo13C ... 2002roles of insulin and glucagon in the regulation of hepatic glycogensynthesis and turnover in humans. J. Clin. Invest. 97, 642–648.21. Petersen, K.F., Laurent, D., Rothman, D.L., Cline,...
... may in uence the shape and volume of the acyl binding pocketand thereby alter the binding mode and affinity of theenzyme for PAA and derivatives thereof, while maintainingthe hydrophobicity of ... quantities of protein utilizing theprinciple of protein dye binding. Anal. Biochem. 72, 248–254.11. Kasche, V., Haufler, U. & Zollner, R. (1984) Kinetic studies on themechanism of the penicillin ... maintaining thestructure of the binding site given the short distances of 3.5 A˚and 3.9 A˚between bF57(CZ) and the substratebinding residues bP22(CB) and bF24(CE2), respectively.Changing...