role of adipose stem cells in obesity

... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched ... that maintain and regulate stem cells [52], Livraghi and colleagues [53] stated that the essential role of stem cell microenvironment in preventing carcinogenesis is by providing signals to inhibit...

Ngày tải lên: 10/08/2014, 10:21

... digestibility-corrected amino acid score of 1.0, which is the same as that of casein and egg protein [28] However, soy proteins contain low methionine/glycine and lysine/arginine ratios compared to casein [29] ... emptying [69] These effects were attributed in part to an increase in circulating levels of cholecystokinin Similarly, in rats fed a hypercholesterolemic diet, ingestion of the alpha subunit of ... IgG4-binding proteins in soybean by immunoblotting with sera from 30 soybean-sensitive patients [88] Ten proteins were detected as IgE-binding proteins and proteins as IgG4-binding proteins, with...

Ngày tải lên: 31/10/2012, 14:59

... secretion of IFN-γ at sites of HSV-1 infection Several clinical case reports indicate that NK cells (a major potential source of IFN-γ) are essential for innate defense against HSV-1 infection in humans ... concluding that NK cells play no role in innate resistance to HSV-1 Likewise, we question the validity of comparisons of HSV-1 infection in mice that exhibit "high" or "low" natural cytotoxicity in ... NK cells and/or professional APCs confer innate resistance to HSV-1 infection via the secretion of IFN-γ at early times p.i The following study was initiated to determine if NK cells provide innate...

Ngày tải lên: 19/06/2014, 08:20

... secretion of IFN-γ at sites of HSV-1 infection Several clinical case reports indicate that NK cells (a major potential source of IFN-γ) are essential for innate defense against HSV-1 infection in humans ... concluding that NK cells play no role in innate resistance to HSV-1 Likewise, we question the validity of comparisons of HSV-1 infection in mice that exhibit "high" or "low" natural cytotoxicity in ... NK cells and/or professional APCs confer innate resistance to HSV-1 infection via the secretion of IFN-γ at early times p.i The following study was initiated to determine if NK cells provide innate...

Ngày tải lên: 20/06/2014, 04:20

... [10], induced visceral hyperalgesia in rats [9] It would be of interest in future study to examine whether serotonin acting on 5-HT1A receptor and tryptase acting on PAR-2 are involved in visceral ... higher in IBS rat colon than in normal colon In IBS rat colon, Fig The number of mucosal mast cells (MMC) in colonic mucosa MMC was stained by 0.5% acidic toluidine blue Bar indicates 100 µm in the ... of diarrheapredominant IBS patients [22], and a significant correlation is found between vicinity of MMC to nerves and the severity of abdominal pain/discomfort symptom of IBS [3] These findings,...

Ngày tải lên: 07/08/2014, 18:20

... advantage of the AIA model is that the time point of induction of arthritis is known, allowing manipulation of CD4+CD25+ Treg cell number in vivo at defined stages in the disease Using depletion of ... a lack of suppression of these cells by Treg cells In accordance with this view, CD4+CD25+ Treg cells are able to suppress innate immune cells in a model of bacteriainduced colitis [20] In later ... in inflammatory colitis in severe combined immunodeficient (SCID) mice [31] Interestingly, two recent papers [32,33] reported an accumulation of functional Treg cells in the inflamed joints of...

Ngày tải lên: 09/08/2014, 06:22

... decreases the infiltrate in joints Furthermore, RA patients relapse shortly after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation and the reinstatement of fully ... sufficient to suppress an ongoing chronic autoimmune inflammatory process This idea had already been hinted at by a series of studies in RA and other chronic autoimmune joint inflammatory diseases such ... the disease is instead very active The authors provided a startling explanation for this apparent incongruence, in their discovery that tissue-infiltrating effector (pathogenic) T cells were less...

Ngày tải lên: 09/08/2014, 06:23

... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit IL-1β induced activation of degradative pathways in MSCs It must be noted that in this study ... positive chondrogenic signaling However, interaction of curcumin and TGF-β signaling in chondrocytes is an interesting point and will require further detailed investigations In the present study we...

Ngày tải lên: 12/08/2014, 14:22

... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines...

Ngày tải lên: 13/08/2014, 23:20

... glycoprotein.39 As a member of the hyaluronanbinding protein family, TSG-6 protein consists of two structural domains: a Nterminal hyaluronan-binding link module, the characteristic domain of this ... Protein in Animal Models of Inflammation The in vivo anti-inflammatory effect of TSG-6 was recognized more than a decade ago first in a skin inflammation model, in which local injection of 10 ... earnest gratitude for holding their standards high and always inspiring and cultivating independence, critical thinking, and open mindedness in my training I thank them for devoting their enthusiasm...

Ngày tải lên: 24/08/2014, 11:56

... CD8 T -cells and the importance of their Th1-inducing responses 239 8.4 Challenges of characterizing monocyte-derived cells into DCs or macrophages 241 8.5 Potential role of Tip-DCs in allergic ... then induce cytolysis of infected cells either by the granule exocytosis pathway through co-ordinated delivery of perforin and granzymes into target cells, or by upregulation of FasL which initiates ... al., 2010) In humans, during the course of an infection or in the presence of macrophage colony-stimulating factor (M-CSF) treatment, there is an increase first of the intermediate cells followed...

Ngày tải lên: 09/09/2015, 18:58

... Aquaporins are integral membrane proteins belonging to a larger family of major intrinsic proteins that form pores in the membrane of biological cells The threedimensional structure of aquaporin ... blood cells 57 4.1.5 Investigation of Ca2+ uptake in sheep red blood cells 60 4.2 Investigation of phosphatidylserine exposure in red blood cells 63 4.2.1 Phosphatidylserine exposure in ... 21 2.4.2 Influence of intracellular Ca2+ on phosphatidylserine exposure 21 2.4.3 Influence of intracellular Ca2+ on protein kinase C 22 2.5 The ageing of red blood cells ...

Ngày tải lên: 15/11/2015, 12:54

... H3: Immunofluorescence staining Scale bars = 50 μm Transplantation of ASCs in spinal cord injury 281 Fig Immunofluorescence staining of ASC group; A1-A3: Glial fibrillary acidic protein (GFAP), ... New canine spinal cord injury model free from laminectomy Brain Res Brain Res Protoc 2005, 14, 171-180 11 Gimble J, Guilak F Adipose- derived adult stem cells: Transplantation of ASCs in spinal ... cells For group V, the injured site was exposed by dorsal laminectomy and 150 μL of PBS was injected into the spinal cord at locations to depths of mm using a 30 gauge needle (middle of the injury...

Ngày tải lên: 07/08/2014, 23:22

... from roof plate cells in the dorsal neural tube and induce formation of neural crest stem cells and dorsal interneurons (Barth et al., 1999) Antagonists of Bmp signaling like chordin, noggin and ... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... Expression of Shh during development Whole-mount in- situ hybridization of Shh in E9.5 days post coitum mouse embryo showing (A) the cross section of the spinal cord (dotted line ’ in B) showing Shh...

Ngày tải lên: 11/09/2015, 10:14

... doubling time of neurospheres in culture, indicating that CSPG stimulates proliferation In addition, CSPG is involved in maintaining the 3dimensional structure of neurospheres Using chondroitinase-ABC, ... Apolipoprotein E Brain derived growth factor Basic helix loop helix Brain lipid binding protein Bromodeoxyuridine C-promoter binding factor1 Cyclin dependent kinase Chondroitin-4-sulfatase Chondroitin-6-sulfatase ... proteoglycan Inner cell mass Iduronic acid IGF binding protein Inducible pluripotent stem cells Janus kinase Keratan sulfate Leukemia inducing factor Lipoprotein receptor related protein Mitogen...

Ngày tải lên: 12/09/2015, 21:26

... transcripts zinc finger protein 42 zinc finger protein 143 zinc finger protein of the cerebellum XI Introduction CHAPTER I INTRODUCTION Introduction CHAPTER I INTRODUCTION Stem cells can be identified ... their binding to GTP or GDP Activated Ras leads to activation of a MAP kinase signaling cascade, including MAP kinase, MAP kinase kinase (MKK), and MAP kinase kinase kinase (MKKK) 16 Introduction ... crucial role for Klf4 in regulating cell proliferation and maintaining genetic stability Recent reports on the reprogramming of somatic cells to pluripotent cells highlight a critical role of Klf4 in...

Ngày tải lên: 14/09/2015, 14:07

... staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... staining of β-tubulin, glial fibrillary acidic protein (GFAP) and galactocerebroside (GalC) in neurosphere-derived cells cultured on poly-L-ornithine- and laminin-coated coverslips displaying ... Immunofluorescence staining of β-tubulin, GFAP and GalC demonstrated positive expressions in a substantial number of cells, suggesting that neurosphere-derived cells were able to differentiate into...

Ngày tải lên: 18/06/2014, 15:20

... These data suggest Stem- Kine supplementation increases circulation of cells capable of giving rise to hematopoieticlineage cells in vitro Stem- Kine augments circulation of cells with EPC phenotype ... were required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic...

Ngày tải lên: 18/06/2014, 16:20

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

Ngày tải lên: 07/08/2014, 18:21

... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof noitulos enilas fo lµ 051 ni 601 ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...

Ngày tải lên: 07/08/2014, 23:22