role of a security manager in enhancing security in an organization
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... investigated. Two point-mutated proteins, with a singlebase substitution of alanine for tryptophan (W14 0A) and alanine for lysine (K13 3A) , and two truncatedfragment proteins, with fragments ... that tryptophan at position140 plays an important role in maintaining protein ter-tiary integrity. Figure 4A shows how tryptophan (loop1) may interact with loop 2 and loop 3. These inter-actions ... that contains onedomain and no disulfide bonds. Its stability in the native state may bemaintained mainly by key amino acids. In this study, two point-mutatedproteins each with a single base substitution...
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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... and isola-ted TAM1 and TAM2 cells, in either of which TAM67was detected (Fig. 6A) . Because TAM67 lacks thetransactivation domain of c-Jun (amino acids 1–122),but retains the DNA binding and ... expression of c-Fos and c-Jun, via the MAPkinase signaling pathway.TAM67 retains the DNA binding and leucine-zipperregion of c-Jun, but it lacks the transactivation domain of c-Jun (amino acids 1–122). ... ADR-induced apop-tosis, suggesting that EGF transmitted the antiapoptotic signal in such a way that it activated AP1 via a MAP kinase signaling pathway. TMK-1cells stably transfected with TAM67,...
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Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
... in cold adaptation of an alkaline phosphataseKonstantinos Mavromatis1,*, Iason Tsigos2,*, Maria Tzanodaskalaki2, Michael Kokkinidis1,3and Vassilis Bouriotis1,21Department of Biology, ... group of Ala261 side-chain could produce stericclashes with the aromatic ring of Tyr269, and theseunfavorable interactions could lead to a decrease of localflexibility and an increased E a value.The ... mutant alkaline phosphatases. (A) Temperature dependence of kcat of TAB5 (d), mutants G26 1A (r), G26 1A/ Y26 9A (j)andE.coli (·) alkaline phosphatases attemperature range 5–25 °C. kcatvalues...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... amplicationbetweenC7UandC8L.Primersinitalicswereusedinthegenotyping of allele M.C1U5Â GAG AGG AAC TGA ACA GAA CAT TG 3ÂC1L 5Â CAA CTG CGT ATT AGT GAA GAA TG 3ÂC2U 5Â AAT CAA ATT TTA TTA TAA GAC C 3ÂC2L5Â AAT AGC TAA TTA GAG ACC AT 3ÂC3U 5Â GGT GTC AAA ... 5Â ATA CAC ACA AAC TCA CAA GG 3ÂMWU 5Â CAA CAT ATT TTA AAT AAA ATT GAC AAT 3ÂC9LM* 5Â ATA AAA ATG GTA TAC CTC ACT TGT*C 3ÂC9UM1 5Â TAA CAA TGA TTC TCT TTC TTT TAG 3ÂC9LM1 5Â AAT CTT TAT TTT ... TGG CAC AAC ATT GTA CAT TCT TGG G 3ÂC9U 5Â GTA TGG AAG TGT GGA ATA GTT T 3ÂC9L 5Â GGA CAC CAC AGA TAT CCA ATA G 3ÂC1011U 5Â CAT AAA ACT AAC AAT ACA TGT 3ÂC1011L 5Â TAG CAG ATA TTG AAA AGG AG...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... lg of DNA per 100 mm dish and2 lg of DNA per 30 mm dish. The ratio of DNA codingdonor to DNA coding acceptor was 1 : 1 or 1 : 2.Membrane preparation and radioligandbinding assayFor binding ... thearginine-rich epitope (217RRRRKR222) of the third intra-cellular loop were exchanged (D2R1: 217AARRKR222,D2R2: 217AAAAKR222, D2R3: 217AAAAAA222), as wellas one variant of the dopamine ... density of the D1MUT (404AA405) wasTable 1. Binding parameters for the dopamine receptors. For dopa-mine D2receptor binding, the statistical significance was evaluatedusing a one-way ANOVA,...
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Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot
... guxx@nidcd.nih.govPresent addresses*School of Veterinary Medicine,Yangzhou University, Yangzhou,Jiangsu, China†Defence Research and DevelopmentCanada-Suffield, Medicine Hat, Alberta,CanadaDatabaseThe nucleotide ... wereobtained via 3070xl DNA analyzer (Applied Biosystems,Foster City, CA) and analyzed with dnastar software(DNASTAR Inc., Madison, WI).Transposon mutagenesis and identification of lgt3 gene In ... Hansen for providing strain O35E,Wenzhou Hong for assisting in the animal challenge,and Robert Morell and Yandan Yang for helping in DNA sequencing. This research was supported by theIntramural...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... of A DNA binding domain B Ligand binding domain Fig. 1. Sequence alignment. (A) Alignment of DNA binding domain (C domain) and itsC-terminal extension. (B) Alignment of ligandbinding domain ... gene assay in in vivo results.Experimental proceduresParasitesThe NMRI strain of S. mansoni was maintained in snails(Biomphalaria glabrata) and Syrian golden hamsters(Mesocricetus auratus ... Yeast one and two-hybrid assays. (A) Yeast one hybridassay showing that SmNR1 contains an autonomous transactivationfunction in A ⁄ B domain. Individual AH109 yeast colonies obtainedfrom an...
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx
... site and plays a critical role in substrate binding [41]. Substrate or sub-strate analogues may anchor this loop upon binding,making a substantial conformational change comparedto the apo structure.Table ... B, Sawka-Dobrowolska W, GancarzR, Kucharska-Zon˜M, Latajka R, Amrhein N, MiziakP & Szczepanik W (2004) Experimental and ab initiocalculated structures of 2-aminoindane-2-phosphonicacid, ... Molecularphenotyping of the PAL1 and PAL2 mutants of Arabid-opsis thaliana reveals far-reaching consequences onphenylpropanoid, amino acid, and carbohydrate meta-bolism. Plant Cell 16, 2749–2771.Tyr-loop in...
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Báo cáo khoa học: Identification of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx
... presence of an additional phosphate group (1032), and additional PEtn moiety (1075), an additional phosphate group and an additional PEtn moiety (1155) and one additional phosphate group andtwo additional ... 227–244.24. Aspinall, G.O., Monteiro, M .A. & Pang, H. (1995) Lipo-oligo-saccharide of Campylobacter lari type strain ATCC 35221.Structure of the liberated oligosaccharide and an associatedextracellular ... The remaining resolved residues in the a- anomeric region at 5.07 and 5.39 p.p.m. and a minorsignal at 5.50 p.p.m. were determined to be gluco-pyranose amino sugars, from the appearance of theirspin...
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Báo cáo khoa học: Identification of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx
... GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGGÓ FEBS 2003 A novel ... GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG 2a+ GACTGCAaCCCCAAAtCGGACAG2b+ CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC2 GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– ... study, interaction of group IIAsPLA2toxins with CaM was evaluated using a set of AtxAmutants, including this quadruple (KKML) mutant, as wellas recombinant DPLA2and its quadruple reciprocal(YIRN)...
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Báo cáo khoa học: Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids Kohsuke Honda, Michihiko Kataoka, Eiji Sakuradani and Sakayu Shimizu doc
... (GAGCTGAATTCTTTGGTCA) containing an EcoRI site (in italics), and dch-II (ATGTAAGATCTGAACTGGACTTCG) containing a BglII site (in italics),cloned into pT7Blue, excised with EcoRI and BglII, andthen ... primer, GGATCCGGACCAGTTGGTGATTTT, and antisense primer, GGATCCTTAGAAAAACTCATCGAGC (BamHI recognition are shown in italics). The amplified KmRwas excised with BamHI andthen inserted into the ... PCR using pADH10 andthe following two oligonucleotides as a template and asprimers, respectively. The sense primer (CAGGAGAATTCAAAATGGG) contained an EcoRI recognition site (in italics), and...
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