role of a security manager in enhancing security in an organization

... investigated. Two point-mutated proteins, with a single base substitution of alanine for tryptophan (W14 0A) and alanine for lysine (K13 3A) , and two truncated fragment proteins, with fragments ... that tryptophan at position 140 plays an important role in maintaining protein ter- tiary integrity. Figure 4A shows how tryptophan (loop 1) may interact with loop 2 and loop 3. These inter- actions ... that contains one domain and no disulfide bonds. Its stability in the native state may be maintained mainly by key amino acids. In this study, two point-mutated proteins each with a single base substitution...

Ngày tải lên: 20/02/2014, 01:20

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Ngày tải lên: 20/06/2014, 07:20

... and isola- ted TAM1 and TAM2 cells, in either of which TAM67 was detected (Fig. 6A) . Because TAM67 lacks the transactivation domain of c-Jun (amino acids 1–122), but retains the DNA binding and ... expression of c-Fos and c-Jun, via the MAP kinase signaling pathway. TAM67 retains the DNA binding and leucine-zipper region of c-Jun, but it lacks the transactivation domain of c-Jun (amino acids 1–122). ... ADR-induced apop- tosis, suggesting that EGF transmitted the antiapoptotic signal in such a way that it activated AP1 via a MAP kinase signaling pathway. TMK-1 cells stably transfected with TAM67,...

Ngày tải lên: 19/02/2014, 06:20

... in cold adaptation of an alkaline phosphatase Konstantinos Mavromatis 1, *, Iason Tsigos 2, *, Maria Tzanodaskalaki 2 , Michael Kokkinidis 1,3 and Vassilis Bouriotis 1,2 1 Department of Biology, ... group of Ala261 side-chain could produce steric clashes with the aromatic ring of Tyr269, and these unfavorable interactions could lead to a decrease of local flexibility and an increased E a value. The ... mutant alkaline phosphatases. (A) Temperature dependence of k cat of TAB5 (d), mutants G26 1A (r), G26 1A/ Y26 9A (j)andE.coli (·) alkaline phosphatases at temperature range 5–25 °C. k cat values...

Ngày tải lên: 22/02/2014, 04:20

Ngày tải lên: 21/03/2014, 22:01

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Ngày tải lên: 09/08/2014, 01:24

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Ngày tải lên: 10/08/2014, 10:20

Ngày tải lên: 10/08/2014, 10:20

Ngày tải lên: 05/09/2013, 16:10

... amplication betweenC7UandC8L.Primersinitalicswereusedinthegenotyping of allele M. C1U 5Â GAG AGG AAC TGA ACA GAA CAT TG 3Â C1L 5Â CAA CTG CGT ATT AGT GAA GAA TG 3Â C2U 5Â AAT CAA ATT TTA TTA TAA GAC C 3Â C2L 5Â AAT AGC TAA TTA GAG ACC AT 3Â C3U 5Â GGT GTC AAA ... 5Â ATA CAC ACA AAC TCA CAA GG 3Â MWU 5Â CAA CAT ATT TTA AAT AAA ATT GAC AAT 3Â C9LM* 5Â ATA AAA ATG GTA TAC CTC ACT TGT*C 3Â C9UM1 5Â TAA CAA TGA TTC TCT TTC TTT TAG 3Â C9LM1 5Â AAT CTT TAT TTT ... TGG CAC AAC ATT GTA CAT TCT TGG G 3Â C9U 5Â GTA TGG AAG TGT GGA ATA GTT T 3Â C9L 5Â GGA CAC CAC AGA TAT CCA ATA G 3Â C1011U 5Â CAT AAA ACT AAC AAT ACA TGT 3Â C1011L 5Â TAG CAG ATA TTG AAA AGG AG...

Ngày tải lên: 22/02/2014, 04:20

... lg of DNA per 100 mm dish and 2 lg of DNA per 30 mm dish. The ratio of DNA coding donor to DNA coding acceptor was 1 : 1 or 1 : 2. Membrane preparation and radioligand binding assay For binding ... the arginine-rich epitope (217RRRRKR222) of the third intra- cellular loop were exchanged (D 2 R1: 217AARRKR222, D 2 R2: 217AAAAKR222, D 2 R3: 217AAAAAA222), as well as one variant of the dopamine ... density of the D 1 MUT (404AA405) was Table 1. Binding parameters for the dopamine receptors. For dopa- mine D 2 receptor binding, the statistical significance was evaluated using a one-way ANOVA,...

Ngày tải lên: 07/03/2014, 03:20

... guxx@nidcd.nih.gov Present addresses *School of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China †Defence Research and Development Canada-Suffield, Medicine Hat, Alberta, Canada Database The nucleotide ... were obtained via 3070xl DNA analyzer (Applied Biosystems, Foster City, CA) and analyzed with dnastar software (DNASTAR Inc., Madison, WI). Transposon mutagenesis and identification of lgt3 gene In ... Hansen for providing strain O35E, Wenzhou Hong for assisting in the animal challenge, and Robert Morell and Yandan Yang for helping in DNA sequencing. This research was supported by the Intramural...

Ngày tải lên: 07/03/2014, 05:20

... of A DNA binding domain B Ligand binding domain Fig. 1. Sequence alignment. (A) Alignment of DNA binding domain (C domain) and its C-terminal extension. (B) Alignment of ligand binding domain ... gene assay in in vivo results. Experimental procedures Parasites The NMRI strain of S. mansoni was maintained in snails (Biomphalaria glabrata) and Syrian golden hamsters (Mesocricetus auratus ... Yeast one and two-hybrid assays. (A) Yeast one hybrid assay showing that SmNR1 contains an autonomous transactivation function in A ⁄ B domain. Individual AH109 yeast colonies obtained from an...

Ngày tải lên: 07/03/2014, 11:20

... site and plays a critical role in substrate binding [41]. Substrate or sub- strate analogues may anchor this loop upon binding, making a substantial conformational change compared to the apo structure. Table ... B, Sawka-Dobrowolska W, Gancarz R, Kucharska-Zon ˜ M, Latajka R, Amrhein N, Miziak P & Szczepanik W (2004) Experimental and ab initio calculated structures of 2-aminoindane-2-phosphonic acid, ... Molecular phenotyping of the PAL1 and PAL2 mutants of Arabid- opsis thaliana reveals far-reaching consequences on phenylpropanoid, amino acid, and carbohydrate meta- bolism. Plant Cell 16, 2749–2771. Tyr-loop in...

Ngày tải lên: 07/03/2014, 12:20

... presence of an additional phosphate group (1032), and additional PEtn moiety (1075), an additional phosphate group and an additional PEtn moiety (1155) and one additional phosphate group and two additional ... 227– 244. 24. Aspinall, G.O., Monteiro, M .A. & Pang, H. (1995) Lipo-oligo- saccharide of Campylobacter lari type strain ATCC 35221. Structure of the liberated oligosaccharide and an associated extracellular ... The remaining resolved residues in the a- anomeric region at 5.07 and 5.39 p.p.m. and a minor signal at 5.50 p.p.m. were determined to be gluco- pyranose amino sugars, from the appearance of their spin...

Ngày tải lên: 08/03/2014, 02:20

... G GAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC 3+ wt GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC 3+ YIRN GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG 3– GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGG Ó FEBS 2003 A novel ... GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG 2a+ GACTGCAaCCCCAAAtCGGACAG 2b+ CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC 2 G GAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC 3+ wt GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC 3+ YIRN GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG 3– ... study, interaction of group IIA sPLA 2 toxins with CaM was evaluated using a set of AtxA mutants, including this quadruple (KKML) mutant, as well as recombinant DPLA 2 and its quadruple reciprocal (YIRN)...

Ngày tải lên: 17/03/2014, 03:20

... (GAGCTGAATTCTTTGGTCA) containing an EcoRI site (in italics), and dch-II (ATGTAAGATCTGA ACTGGACTTCG) containing a BglII site (in italics), cloned into pT7Blue, excised with EcoRI and BglII, and then ... primer, GGATCCGGACCAGTTGGT GATTTT, and antisense primer, GGATCCTTAGAAAA ACTCATCGAGC (BamHI recognition are shown in italics). The amplified Km R was excised with BamHI and then inserted into the ... PCR using pADH10 and the following two oligonucleotides as a template and as primers, respectively. The sense primer (CAGGAGA ATTCAAAATGGG) contained an EcoRI recognition site (in italics), and...

Ngày tải lên: 17/03/2014, 09:20