rhizogenes with high efficiency

... regenerants of all three genotypes showed high mobility of mPing, both excisions and insertions varied markedly among them (Figure 1), with V27 and V14 showed markedly higher numbers than those of R09 ... among all kinds of TEs, MITEs are most closely associated with plant genes [6-9] This, together with their propensity to accumulate to highcopy numbers (relative to other types of Class II or ... loamy soil mixed with sand, where they were maintained in a greenhouse conditions at ~25°C with mild illumination The plantlets survived and developed into healthy plants at high frequencies...

Ngày tải lên: 12/08/2014, 03:20

... sufficiency of this approach in section five, comparing it to the knowledge already available within WordNet We conclude with some final remarks in section six Functional Context-Sensitive Categories We take ... WordNet, and drinks can be ”stiff” 60 too) Disambiguation is trivial for nouns with just a single sense in WordNet For nouns with two or more fine-grained senses that are all taxonomically close, such ... frequency of co-occurrence with that noun in our simile data) More specifically, we should expect that ratings estimated via these simile-derived properties should correlate well with the independent...

Ngày tải lên: 08/03/2014, 02:21

... version of Morphy, with a 2.5 times smaller lexicon, had been 89.2%, 95.9%, 86.9%, and 75.8% The Disambiguator Since the morphology system only looks at isolated word forms, words with more than ... main reason is that with large tag sets, the sparse-data-problem can become so severe that unsupervised training easily ends up in local minima, which can lead to poor results without any indication ... Morphy-generated full form lexicon The coverage of the current version of Morphy was evaluated with the same test corpus that had been used at the Morpholympics This corpus comprises about 7.800...

Ngày tải lên: 17/03/2014, 07:20

... shorter, resolution of radar is higher It is almost impossible if use a complicated circuit with a total long time delay of electronic components 3.2 Phased-code modulator With the method of BPSK modulation ... results are Barker code 13 elements with bit duration 3.24 µs, BPSK signal frequency IF 10.3 MHz, stable local oscillator 800 MHz-900 MHz with frequency error 10 Hz, high microwave power D.T Kien et ... logarithmic function for high amplitude signals Therefore, a large signal does not saturate the Logarithmic Amplifier AD6006 is used to make this amplifier Good features of the AD6006 are high linearity...

Ngày tải lên: 22/03/2014, 11:20

... association with MCF7/Her2-18 cells (Figure 4e, f), reduced nanoparticle association with MDA-MB-231 cells (Figure 4g), and none with CHO cells (Figure 4h) These results are consistent with the ... calculated by comparison with a range of microspheres with known binding capacities As expected, MCF7 breast cancer cells engineered to overexpress Her2-18 have a very high number of Her2-binding ... magnetic detection with SQUID sensors Furthermore, antibodyconjugated nanoparticles bind in greater numbers to breast cancer cells expressing high levels of Her2 compared with cells expressing...

Ngày tải lên: 22/03/2014, 17:20

... patients with DS TB, MDR TB and XDR TB, respectively) (Table 2) (Figs 1-3) The frequency of GGO and pleural effusion was significantly higher in patients with DS TB as compared to patients with MDR ... patients with MDR TB as compared to patients with DS TB (p = 0.049) (Table 6) Multiple cavities were also more commonly observed in patients with MDR TB (40%) as compared to patients with DS TB ... Comparisons of Demographic Data among 141 Patients with Drug-Sensitive Tuberculosis, 53 Patients with Multidrug-Resistant Tuberculosis and 15 Patients with Extensively Drug-Resistant Tuberculosis DS...

Ngày tải lên: 22/03/2014, 18:20

... of us who want to experiment with RFID, the problem is that the technology is almost always black boxed That is, the inner workings of a tag and its interaction with a reader is hidden from view, ... (http://www.sonmicro.com/) Note: The Phidget RFID reader does not work with the tags created in this project, as it uses a different frequency for communication with the tags (125 kHz) Advanced material (see Figure ... RFID readers transmit an electromagnetic (EM) field with their reader antenna This EM field induces a current in the antenna for all RFID tags within reading distance This induced current activates...

Ngày tải lên: 18/04/2014, 09:11

... precipitated and extracted 2× with brine and 3× with deionized water, and the product was dried by rotary evaporation mL of the product was redissolved in mL of DMF, and then reacted with ~0.1 eq of Mn ... Advanced Materials 2007, 19:33-60 Josephson L, Tung CH, Moore A, Weissleder R: High- efficiency intracellular magnetic labeling with novel superparamagnetic-Tat peptide conjugates Bioconjug Chem 1999, ... to pellet without an ultracentrifuge, and are very slowly pelleted in proximity to a T-field strength neodymium magnet These observations have been suggested by other groups working with colloidal...

Ngày tải lên: 11/08/2014, 00:23

... circulation is present in high molecular weight complexes, mostly with IGFBP-3 [17] There are six IGFBPs in circulation that can bind IGFs with high affinity and interfere with antibody-based detection, ... added to each CNTFET circuit and incubated for hr at RT followed by blocking with 0.001% BSA, washing with diH2O and drying with N gas For the assays, a baseline impedance value for the circuit was ... important since the breast cancers from populations of women at high risk have elevated levels of circulating IGF-1 (e.g women with BRCA1 mutations) are more difficult to treat/manage due to...

Ngày tải lên: 11/08/2014, 00:23

Ngày tải lên: 11/08/2014, 08:20

... with the EMSA PDTC reduced lung lipid peroxidation as assessed by levels of malondialdehyde, without reducing the neutrophil oxidant production [17] Stress response The stress response is a highly ... inflammatory lung disease, with abundant production of thick, tenacious, protease-rich and oxidant-rich purulent airway secretions that are difficult to clear, even with physiotherapy In the search ... concert with these observations, it has been reported that systemic blood loss affects NF-κB regulatory mechanisms in the lungs For instance, NF-κB is activated in the lungs of patients with ARDS...

Ngày tải lên: 12/08/2014, 19:21

... hours with µg/ml lipopolysaccharide–endotoxin (Pseudomonas aeruginosa) in normoxia (21% O2–5% CO2) or in hypoxia (1.8% O2–5% CO2) The AMs exposed to lipopolysaccharide–endotoxin in hypoxia had higher ... function Compared with control lungs Available online http://ccforum.com/content/7/1/47 hours after transplant, pyrrolidine dithiocarbamate-treated lungs displayed significantly higher oxygenation, ... therapy in paralyzed patients with acute lung injury Crit Care 2001, 5:81-87 Kheradmand F, Wiener-Kronish JP, Corry DB: Assessment of operative risk for patients with advanced lung disease Clin...

Ngày tải lên: 12/08/2014, 19:21

... could be determined (fig 1B) This result is in agreement with earlier studies using CRFK cells expressing human TRIM5α after challenge with VSV-G pseudotyped HIV2ROD [9] but shows in addition ... isolates) variants studied In comparison to the experiments performed with pseudotyped particles, a weaker restriction to infection with HIV-1NL4.3 versus HIV1[NL4.3] was observed NL4.3 envelope pseudotyped ... T, Perez-Caballero D, Cowan S, Bieniasz PD: Cyclophilin interactions with incoming human immunodeficiency virus type capsids with opposing effects on infectivity in human cells J Virol 2005, 79:176-183...

Ngày tải lên: 13/08/2014, 09:20

... oligomer that has a neutral peptide-like backbone with nucleobases that allows for the molecules to hybridize to complementary DNA (cDNA) strands with high affinity and specificity.26 The immobilized ... semiconducting and its high current carrying capacity This interest continues as other remarkable properties are discovered, such as their exceptionally high mechanical strength,80,81 high thermal conductivity82 ... analysis were optimized With optimized conditions, a linear response for HRP from 0.003 - 0.1 U mL-1 and a detection limit of 1.25 × 10-15 mol (in 25 µL) were obtained with a good precision (relative...

Ngày tải lên: 12/09/2015, 09:28

... coefficient above 0.8 indicate a highly accurate and predictive assay Even when a single CpG site already provides a highly accurate measurement - as is the case here - it is highly recommended to use ... methylation level, hierarchical clustering, or K-means clustering with K = In all cases, the DNA methylation profiles in the subset with the higher average methylation levels are labeled as methylated ... biomarkers subsequently fail due to technical problems or lack of robustness, all high- scoring assays are validated with respect to their susceptibility to experimental noise, measurement errors...

Ngày tải lên: 09/08/2014, 20:20

... the length of line c (c) The HpaII count correlates negatively with the degree of methylation, with more counts occurring at loci with less methylation (d) Transformation of the data to the B ... can see two Page of 11 major groups of values in the plot (Figure 2a), separated into loci with high or with minimal HpaII counts This plot helped us to develop a new method for normalizing HpaII ... Cruz (UCSC) genome browser visualization as wiggle tracks, with higher B angle values defining less methylated loci Methylated loci with zero values that would be otherwise difficult to visualize...

Ngày tải lên: 09/08/2014, 20:21

... found in each sample is reported, with the error bars, in Fig Within the somatic cell lineage, a higher number of R markers were found in leukocytic DNA when compared with fibroblastic DNA (94.8% ... DNA, time and temperature applied to the digestion reaction were in accordance with the recommendation provided with the product Digested DNA samples were ethanol precipitated and separated from ... treated with a methylation-sensitive enzyme, HpaII (Invitrogen ® , Hong Kong) The digestion solution consisted of autoclaved de-ionized water and buffer solution plus bovine serum albumin provided with...

Ngày tải lên: 12/08/2014, 18:22

... encouraging since we found that studies with positive results or with 1-3 polymorphisms were not cited more frequently compared with studies with negative results or with > polymorphisms In literature, ... together with a high number of other polymorphisms We also tried to assess any differences in journal impact factor or citation frequency among positive versus negative reports Due to the high number ... as “publication”) bias refers to the greater likelihood of a study with positive results to be published compared with studies with negative results [2,3] Publication bias (“false-positive” reporting)...

Ngày tải lên: 25/10/2012, 10:51

... associated with -28C/G RANTES was estimated for each study by odds ratio (OR) with 95% confidence intervals (95%CI) For all studies, we evaluated the risk of the variant genotypes (GG/CG), compared with ... significantly increase the risk of asthma, which is consistent with the hypothesis from previous studies that the -28C/G polymorphism with higher activity of RANTES may be more important in asthma ... polymorphisms were associated with an increased asthma risk in different genetic models As shown in Table 2, both the variant homozygote (-28GG) and heterozygote (-28CG) were associated with a significantly...

Ngày tải lên: 26/10/2012, 09:39

... Shibata A, Katsuda N, et al Subjects with TNF-A-857TT and -1031TT genotypes showed the highest Helicobacter pylori seropositive rate compared with those with other genotypes Gastric Cancer 2003; ... (OR=1.95, 95% CI 1.12 -3.40) Seropositive individuals with the PTPN11 G/G and Gab1 G/A+A/A demonstrated the highest risk of gastric atrophy with significance relative to PTPN11 G/A+A/A and Gab1 ... significantly higher in the gastric cancer cases than in the healthy controls (100% vs 55.1%, p

Ngày tải lên: 31/10/2012, 15:37