... CI-ELISA was performed to further enhance the specificity of the antibody The antibody was preincubated with different concentrations of CatE and CatD, before a standard ELISA was performed to detect ... different cell lines used for CatE and CatD determination For immunochemical determination of subcellular localization of CatE and CatD, western blot analysis was performed No CatE was recovered ... almost no effect on CatD activity (Fig 4B) This approach for depleting proteinase activity from complex biological samples is flexible and can be used for other proteinases as well Activity of Cat...
Ngày tải lên: 07/03/2014, 09:20
... filter) The amplifier gain was set at 1.00 for forward scattering; the detector voltage was set to E00 for forward scattering and 600 V for FL1, and the forward-scattering threshold was set at 52 ... Fellowship for Young Scientists from the Japan Society for the Promotion of Science, and in part by Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for ... prototrophic a cell [11], were utilized as parental strains for construction of our system The genetic modifications shown in Table were performed for BY4741 only, and the recovery of pheromone signaling...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo hóa học: " A New Approach for Estimation of Instantaneous Mean Frequency of a Time-Varying Signal" doc
... time and frequency, therefore it is tempting to try to use it to measure the spread of frequencies with time Unfortunately, the spread of the IF of the WVD is only positive for certain types of signals ... TFD suitable for estimating the IF of a signal ADAPTIVE TIME-FREQUENCY DISTRIBUTIONS The purpose of this paper is to explore the best available TFD for estimating the IF of a signal For simple ... will satisfy all the criteria and still will give desired performance for time-varying signal analysis and feature extraction Performance of Cohen’s class TFDs depends totally on the kernel function...
Ngày tải lên: 23/06/2014, 01:20
Báo cáo y học: "A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology" ppt
... 800 U/ml GM-CSF and 1,000 U/ml IL-4 was added The cells were incubated for 4–5 days and matured for 20–24 h and analyzed for mature DC content and function as above Determination of mDC yield ... bottles or T-175 culture flasks and adhered for 2.5 h (1 rpm for the roller bottles) in the incubator The non-adherent cells were collected and stained for CD14 and CD3 expression Adherence of monocytes ... or in T-175 static flasks as before using ITIP maturation The floating cells were harvested and stained for DC maturation markers without fixation Immediately before FACS analysis µg/ml 7-ADD...
Ngày tải lên: 11/08/2014, 10:23
optimization of protein and rna detection methodologies and a new approach for manipulating protein activity in living cells
... availability and huge effort required for the development of specific pharmacological agents, new general methods for protein modulation would be highly desirable During selection for optimized tetracysteine ... the mammalian cell-based library approach for optimization of the tetracysteine motif In an effort to further optimize the ReAsH binding tetracysteine peptide, a new library, RRL2, was devised, ... required for optimal biarsenical-tetracysteine contrast, primary human foreskin fibroblast proliferation slows down for a while, dividing only once in 72 hours compared to two divisions for untreated...
Ngày tải lên: 13/11/2014, 10:46
A new approach to financial regulation: the blueprint for reform potx
... therefore taking forward the new product intervention power It is described in new sections 137C and 137D of FSMA, contained in clause 19 of the draft Bill, which substitutes new Part 9A for Part ... response, the Government is therefore bringing forward revised objectives for the PRA New section 2B of FSMA (the PRA’s general objective) restates the PRA’s responsibility for financial stability in ... 15 June, the Government endorses their approach The Government will await the Commission’s complete report before taking final decisions Reforms taken forward will need to meet the following...
Ngày tải lên: 29/03/2014, 18:20
báo cáo hóa học:" A practical approach for the validation of sterility, endotoxin and potency testing of bone marrow mononucleated cells used in cardiac regeneration in compliance with good manufacturing practice" docx
... Pharmacopoeia; for noncompendial items and new drugs, the endotoxin limit should be calculated by the user The Maximum Valid Dilution (MVD) provides an upper bound for dilution that still provides for endotoxin ... structure for the wide range of stem-cell-based products that may be developed to regenerate damaged tissues Main scope of the regulations is to establish clear classification criteria for many new ... in vivo assays Cells were evaluated for hemat- Page of (page number not for citation purposes) Journal of Translational Medicine 2009, 7:78 opoietic colony-forming unit (CFU), and assessment of...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo hóa học: " A new approach to geographic routing for location aided cluster based MANETs" pot
... improve performances in IWCA [11], FWCA [12] GDMAC improves the performance by introducing a cluster density parameter for the whole network WCA and its derived algorithms provide better performance ... The new node’s HELLO message is acknowledged by cluster head (CH) JOIN A new node joins as member in the cluster after cluster head (CH) is activated by sending JOIN message CH_NEWNODE The new ... and Forward the packet to the node N and N forwards the packet to Node D Endif Endif Else Node S forwards the packet towards Node D Endif When there is mobility of a node inside a cluster for...
Ngày tải lên: 21/06/2014, 03:20
Báo cáo y học: "Implementing the International Liaison Committee on Resuscitation guidelines on hypothermia after cardiac arrest. The German experience: still a long way to go" potx
... out-of-hospital cardiac arrest with induced hypothermia N Engl J Med 2002, 346:557-563 Anonymous: Mild therapeutic hypothermia to improve the neurologic outcome after cardiac arrest N Engl J ... PT, Nolan JP, Okada K, et al.: Therapeutic hypothermia after cardiac arrest: an advisory statement by the advanced life support task force of the International Liaison Committee on Resuscitation ... indications recommended by published guidelines and each hospital should generate or adopt written SOP for the indication and clinical use of therapeutic hypothermia [3] Even if the optimal duration...
Ngày tải lên: 12/08/2014, 23:23
A facile construction of the tricyclic 5 7 6 scaffold of fungi derived diterpenoids the rst total synthesisof (±) heptemerone g and a new approach to danishefsky s intermediate for a guanacastepene
... Scheme Highlights of the proposed scheme for the synthesis of We now report the first total synthesis of heptemerone G (2) and, en route, a new synthetic approach to compound (which is a guanacastepene ... and oxo groups in the intermediate were designed to serve as bridgeheads for forming ring C via the keto-ester Further transformations of into and will require diastereoselective reduction of the ... spectrum, presumably due to the conformational flexibility of this compound.2a,3a No spectrum of the natural compound was available for a direct comparison To complete the formal synthesis of guanacastepene...
Ngày tải lên: 26/01/2016, 09:27
Báo cáo y học: "Rasburicase represents a new tool for hyperuricemia in tumor lysis syndrome and in gout Lisa Cammalleri and Mariano Malaguarnera"
... than uric acid It may impair renal function and improve stone formation Its formulation is oral since 1966 Since 1999 a new intravenous formulation (not yet available in Italy) was introduced in ... standard drugs used for TLS The classic approach to TLS fails in prevention of acute renal failure in over to 25% of patients [42] Gout Even though rasburicase is approved for treatment and prophylaxis ... acid and acute arthritic attack PEG-uricase, the long-acting form, seems to better control gout that non-pegylated form The need for a daily administration and the increased probability of development...
Ngày tải lên: 31/10/2012, 14:59
A New Approach to Quantum Theory
... THESIS A New Approach to Quantum Theory This page intentionally left blank FEYNMAN'S THESIS A New Approach to Quantum Theory Editor Laurie M Brown Northwestern University, USA WWorld Scientific NEW ... — A New Approach to Quantum Theory its usefulness in these diverse fields of physics, the path-integral approach brings a new fundamental understanding of quantum theory Dirac, in his transformation ... defined by δ(t)f (t)dt = f (0) for any continuous function f δF The function q(σ) for which δq(t) is zero for all t is that function for which F is an extremum For example, in classical mechanics...
Ngày tải lên: 06/11/2012, 11:21
A new approach to semantic and syntactic functions of English adjectives – A contrastive analysis with their Vietnamese equivalents
... Title: A new approach to semantic and syntactic functions of English adjectives A contrastive analysis with their Vietnamese equivalents (Graduation paper submitted in partial fulfillment for B.A ... writer chooses studying one aspect of English grammar for the graduation paper, particularly adjectives in English The writer decided to study a new approach to semantic and syntactic functions of ... isolation because the form of a word doesnt necessarily indicate its syntactic function Nor can we identify a word as an adjective merely from its potentials for inflexion (1973,114) As for them, a word...
Ngày tải lên: 10/04/2013, 14:46
a decentralized approach for implementing identity management in cloud computing
... single vertex that forms for Gt in list[G’, G”] if n(Gt) < then undo delete operation of this time and continue c) with the next edge; end if end for //do the same operation for each new graph just ... detection approach to take advantage of anomaly detection’s ability to detect new attacks, but without the approach s accompanying high rate of false positives There are some strategies for implementing ... and get a new graph G1 //delete edges weights from small to large, one edge a time if P(G1) = P(G0) then goto c); else { now two new graphs form, list[G’, G”]; //make sure there isn’t a new graph...
Ngày tải lên: 31/07/2013, 09:43
Tài liệu The Book Of Personal Transformation - How To Use Ancient Wisdom To Create A New Life For Yourself docx
... blame anyone anymore There are three types of forgiving: Forgiving those who have hurt us Forgiving ourselves for hurting others Forgiving ourselves for hurting ourselves To open our heart to love, ... Personal TRANSFORMATION How to Use Ancient Wisdom to Create a New Life of Success and Happiness For Yourself Dr Tim Ong M.B.B.S Personal TRANSFORMATION How to Use Ancient Wisdom to Create A New Life ... this new one in order to rise to a new level of achievement CREATE HABITS OF SUCCESS As we have mentioned earlier, old habits die hard To change old habits and replace them with new ones, new effort...
Ngày tải lên: 15/12/2013, 06:15
A new algorithm for enumeration of minimum cutsets of graph by branch addition
... routine is performed for node sets with 3, 4… (n-m-1) nodes and the minimum cutsets are evaluated, respectively The flowchart of the new algorithm is shown in figure START Load Network's Information ... enumeration approach is performed Calculating other output node starts only after all the minimum cutsets of the previous output node has been calculated To calculate the minimum cutsets for one ... Landers, "A Recursive Approach for Enumerating Minimal Cutsets in a Network," IEEE Trans on Reliability, Vol 43, No 3, September 1994 Heejong Suh, Carl K Chang, "Algorithms for the Minimal Cutsets...
Ngày tải lên: 03/01/2014, 19:35
Tài liệu VIRTUAL REALITY - A NEW TECHNOLOGY FOR THE MECHANICAL ENGINEER docx
... need for realism, it is imperative to maintain a reasonable performance for the VR application Here "performance" refers to the response of the virtual environment to the user's actions For example, ... could be used for VR applications Currently, all major UNIX workstation vendors have specific platforms targeted to the VR market These workstations usually have a enhanced graphics performance and ... render per second Typical speeds for UNIX-based Silicon Graphics machines range from 60,000 Tmesh/sec (Triangular Mesh) for an Indigo2XL to 1.6 million Tmesh/sec for a Power Onyx/12.3 The second...
Ngày tải lên: 23/01/2014, 07:20
Tài liệu A New Era for Conservation docx
... for improving forest resiliency as well as ensuring the greatest opportunity for success in efforts to accommodate changes in forests due to climate change Two well-established conservation approaches ... customized for the given situation, scientists suggest that forest managers implement measures to reduce susceptibility to severe wildfires as well as develop new strategies to manage forests for altered ... within a forest ecosystem, to the best extent possible, to help maintain ecosystem function (Noss, 2001) These approaches can be considered in efforts to protect existing forests as well as efforts...
Ngày tải lên: 25/01/2014, 20:20
Tài liệu Focusing Resources on Effective School Health: A FRESH Approach for Achieving Education for All doc
... priority targets for renewed efforts to achieve Education for All, are likely to be the least healthy and most malnourished of new school enrolees To protect their investment in efforts to increase ... FOR ACHIEVING EFA I The Rationale for School Health The commitments made through adoption of the Dakar Framework for Action during the World Education Forum (Dakar, 2000) are revitalising efforts ... Education for All The expanded commentary on the Dakar Framework for Action describes three ways that health relates to Education for All First, as an input and condition required for learning;...
Ngày tải lên: 14/02/2014, 09:20
Tài liệu The New Face oF GoverNmeNT: How Public Managers Are Forging a New Approach to Governance pdf
... The New Face oF GoverNmeNT How Public Managers Are Forging a New Approach to Governance © 2009 by Taylor & Francis Group, LLC American Society for Public Administration American Society for Public ... Laboratories approach, a four-level transformation model that focuses on identifying a transformation trigger, and an eight-factor public management model A Need for Transformation Testifying before ... through social integration Forging the New Model of Governance The great change movement underway in government has made it possible for public managers to forge a new model of governance, one...
Ngày tải lên: 15/02/2014, 20:20