resolvase based in vivo expression technology

... that removal of the N-terminal helices and while leaving the interacting a-helix intact, may destabilize the proteins leading to spurious protein–protein or/and protein–DNA interactions The crystal ... mobility binding assays with increasing concentrations of control Ydc2 and Ydc2-ND35 (A) or Ydc2-ND35/CD15 (B), as indicated, visualized by phosphorimaging (C) Binding reactions with increasing concentrations ... demonstrated [38] suggesting that recombination intermediates and enzymes that resolve them could be involved in maintaining the stability and inheritance of mitochondrial genomes in human cells These...

Ngày tải lên: 20/02/2014, 11:20

... factors, including the longevity of the injected protein and the level of free G-actin in the circulation at sites of inflammation inhibiting the DNase I activity Our data obtained by a transgenic expression ... induced in mice by the administration of a single high dose of LPS This induces a pulse of nucleosomes and chromatin in the plasma within the first 12 hours post-injection accompanied by an increase ... from Pharmingen-Becton Dickinson (San Diego, CA, USA) Biotinylated antibodies were detected using an allophycocyanin-conjugated streptavidin antibody (Pharmingen-Becton Dickinson) Staining was...

Ngày tải lên: 09/08/2014, 07:20

... in a model that would test its potential for application in vivo Since retroviral vectors are best suited for ex vivo gene transfer and one of their typical uses in clinical trials has been in ... vector silencing was observed in this compartment In addition, in vivo treatment with 5-aza did not increase the number of eGFP-positive cells in the bone marrow compartment, corroborating the idea ... removal of the drug would result in the reduction in vector expression The insertion of the retroviral vector may induce the unwanted expression of a neighboring oncogene In the case of pCLPG, the enhancer...

Ngày tải lên: 12/08/2014, 04:21

... while using stairs; = need one hand support in walking; = need two hands support in walking; = need two hands support in walking but is limited to 10 m; = need two hands support in walking but ... proliferation in transgenic mice [32], and enhances infectivity and persistence in HTLV-1-inoculated rabbits [34] In this study, we investigated whether HTLV-1 HBZ mRNA expression is associated with clinical ... distinct bands of over kb were observed in EcoRI digestion in samples from two ATL patients, indicating at least the biclonal integration of HTLV-1 proviral DNA The incidence of multibands in...

Ngày tải lên: 12/08/2014, 23:20

... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... glycine-rich linker is flexible, which allows the specific engineering of hinge regions into proteins to achieve desired functional motions [17] We used a glycine-rich short peptide including 15 amino ... fusion protein in inclusion body was refolded in urea gradient refolding buffer And then, the refolded protein was incubated with enterokinase to remove the His-tag The fusion protein without...

Ngày tải lên: 25/10/2012, 11:10

... explain in part the decreased expression of c-Jun found in chronic19 vitamin A-deficient rats The molecular mechanism that explains the decrease in C-H-Ras expression found in chronic-vitamin A ... controls, while in acute hypervitaminosis the binding was significantly higher These changes in the RAR–DNA binding is not due to different levels of the retinoic acid receptor induced by the treatment, ... as RAR expression was similar in control, vitamin A-deficiency and hypervitaminosis (Fig 5) rats An antibody against a protein unrelated to vitamin A was used as a mock control and binding was...

Ngày tải lên: 08/03/2014, 08:20

... mixture Semithin sections (2 lm) were stained with toluidine blue; thin sections were obtained with a Reichert Ultracut E (Reichert, Wien, Austria), stained with lead citrate, and examined in a transmission ... UCP1 in unilocular adipocytes in subcutaneous than in epididymal fat and suggest that UCP1 is not capable of inducing conversion of a unilocular into a multilocular adipocyte UCP1-induced increase ... during ageing in either genotypes However, as indicated by ANOVA, there was a main effect of genotype in both depots, with transgenic animals showing higher levels of the transcripts Within different...

Ngày tải lên: 31/03/2014, 15:20

... (group A) is in agreement with previous cloning studies in mouse CD8+ lymphocytes [15,18] showing that a few highly expressed microRNAs prevailed in frequency Interestingly, these microRNAs include ... group C), indicating intra-cluster differential expression Transgenic overexpression of miR-17~92 cluster in mouse lymphocytes was shown to induce lymphoproliferative disease [16] High expression ... response to pathogens in vivo [12], demonstrating that miRNAs are not only important for lymphocytes differentiation, but also for their functions in the periphery Interestingly, single miRNA such...

Ngày tải lên: 18/06/2014, 19:20

... techniques for assessing in- vivo PF joint motion have included dynamic CT imaging [34] and single-plane fluoroscopic imaging combined with shape matching [35] Dynamic CT imaging has limitations ... http://www.josr-online.com/content/3/1/38 11 12 13 14 15 In summary, this model -based tracking approach is a noninvasive technique for accurately measuring in- vivo PF joint motion during dynamic activities ... coordinate measuring machines [10] These studies have provided valuable insight into factors that may influence patellar tracking, but cadaveric experiments are unable to duplicate the in- vivo...

Ngày tải lên: 20/06/2014, 01:20

... (group A) is in agreement with previous cloning studies in mouse CD8+ lymphocytes [15,18] showing that a few highly expressed microRNAs prevailed in frequency Interestingly, these microRNAs include ... group C), indicating intra-cluster differential expression Transgenic overexpression of miR-17~92 cluster in mouse lymphocytes was shown to induce lymphoproliferative disease [16] High expression ... response to pathogens in vivo [12], demonstrating that miRNAs are not only important for lymphocytes differentiation, but also for their functions in the periphery Interestingly, single miRNA such...

Ngày tải lên: 20/06/2014, 03:20

... 255 Acetaminophen, ramipril, omeprazole, aspirin, atorvastatin 12 M Caucasian 4073 Insulin, nifedipine, glipizide, lisinopril, metformin, Vicodin, Flexeril, indomethacin, sulfasalazine 13 F Caucasian ... CTNNAL1* Catenin (cadherin-associated protein), alpha-like 3.0 2.3 2.3 2.1 Analogous to α-catenin which inhibits βcatenin Á catenin inhibits wnt/catenin pathway KREMEN1 Kringle containing transmembrane ... Metformin, glipizide, atorvastatin, lisinopril, nifedipine, Lantus insulin, Novo Log, sulfasalazine, indomethacin M Caucasian 558 Ibuprofen F Caucasian 11383 Piroxicam, indomethacin 10 F Caucasian 7115...

Ngày tải lên: 09/08/2014, 14:22

... were based on significantly distinct cell cycle distributions between in vitro and in vivo infected clones and, surprisingly, also between uninfected clones derived from in vitro versus in vivo infection ... [25] Co-cultures were maintained for 28 days in six-well plates in ml of RPMI 1640 medium (Gibco, Paisley, United Kingdom) containing 100 U/ml of recombinant interleukin in the absence of exogenous ... typical cellular features of genetic instability that characterize in vivo infected CD4+ clones After having characterized in vitro and in vivo infected clones for tax expression and cell morphology,...

Ngày tải lên: 12/08/2014, 23:23

... signaling in chondrosarcoma proliferation, which is consistent with the fact that very few tumors express AR In addition, in spite of positive immunohistochemical staining for ESR1 protein in all in ... show any response In the current study, we aimed to gain more insight into the possibility of treating chondrosarcoma patients with hormonal therapy by further investigating the expression of the ... estrogen signaling-inhibiting drugs on the progression of chondrosarcoma cells in vitro We demonstrated expression of ESR1 in a large proportion of various types of cartilaginous tumors In conventional...

Ngày tải lên: 13/08/2014, 15:21

... performed by heating sections in pH 6.0 citrate buffer Sections were then rinsed in PBS and incubated in blocking buffer (5% bovine serum albumin, 0.3% Triton X-100, 10% normal goat serum, in PBS) for ... principal component (PC1) MTB/ TRAS mice uninduced (open triangles) or induced (filled triangles) for 48 or 96 h were used for training, while the remaining MTB/TRAS time points and MTB uninduced ... Taken together, our results indicate that SVD regression can detect activation of endogenous pathways in vivo, thereby providing novel insight into cell signaling in vivo Genome Biology 2008, 9:R180...

Ngày tải lên: 14/08/2014, 21:20

... oromandibular or lingual activities that may include, alone or in combination: jaw clenching, bruxism, tooth grinding, tooth tapping, cheek, lip or tongue biting, tongue pushing against teeth, licking lips, ... TMJ clicking and severe bruxism This comes in agreement with the findings of another study examining the association between para-functions (diurnal clenching and/or grinding and nail-biting) and ... 3.1.4!Overall!3D!scanning!system!performance! !127! 3.2#Clinical#findings# #130! 3.2.1!Survey!of!secondary!tooth!wear!referrals!findings! !131! 3.2.2!Clinical!findings!of!questionnaire!and!3D!analysis!over!a!period!of!one!year!...

Ngày tải lên: 22/12/2014, 18:14

... variety of loading strategies, mainly including (a) chemical binding to external surface, (b) π-π stacking on both internal and external surface, (c) encapsulation into inner cavity, etc., based on ... distribution of cisplatin and Pt(IV) complex in vivo, cisplatin and Pt(IV) complex were entrapped within various MWCNTs (i.e pristine, carboxylated, amidated), and then injected intravenously into mice The ... Chemistry, National University of Singapore, for kindly providing platinum(IV) compounds, and Prof RAMAPRABHU Sundara, Indian Institute of Technology, Madras, India, for providing ultrapure multi-walled...

Ngày tải lên: 08/09/2015, 18:10

... corresponding in vivo index Ein vivo that were computed from histological scores The result infers that our in vitro cell -based system has some predictability of the in vivo response • In Chapter ... single parameter is used For example, one study measured the binding affinities of 19 opioid drugs A ranking based on the magnitude of these binding affinities was done for developing labeling ... Average intensity of all pixels in Ch2 within Ch1 object mask Total intensity of all pixels in Ch2 within Ch2 but not in Ch1 object mask Average intensity of all pixels in Ch2 within Ch2 but not in...

Ngày tải lên: 10/09/2015, 15:48

... transistors per chip have been increasing from 103 in year 1972 to more than 109 in today’s leading-edge technology, as shown in Fig 1.2 However, as the technology advances into sub-32 nm regime, fundamental ... Byung-Jin Cho and Prof Ganesh Samudra for their tremendous contribution in establishing and maintaining SNDL in both its facilities and traditions Mr Yong Yu-Fu, Mr Patrick Tang, Mr O Yan Wai Linn ... interface played an important role in the voltage linearity of the MIM stack An innovative dielectric structure is developed by intentionally inserting a thin SiO2 layer between the lanthanoid...

Ngày tải lên: 14/09/2015, 08:36

... anti-inflammatory activity is measured by the model of carrageenin-induced edema in hind paw of the rat Results: 10 Polyoxychalcones were obtained with good yields and the results of in vivo ... affect to the in vivo anti-inflammatory activity of tested chalcones The polyoxychalcone which possessing a hydroxy group at position C2 of A ring and at least one group methoxy in B may be consider ... nhóm methoxy vòng B làm tăng tác dụng kháng viêm in vitro in vivo chalcon - Nhóm 2’-hydroxy có ảnh hưởng định đến tác dụng kháng viêm in vitro in vivo chalcon Tăng tác động kháng viêm cho chalcon...

Ngày tải lên: 15/11/2012, 14:03

... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson, ... i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma...

Ngày tải lên: 23/04/2013, 21:38