... QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA). Plasmid pET-1TK was used as template and Kleb(HtoA)fw (5¢-GCTTAGCCGCGCCGGCATTCG) and Kleb(HtoA)rv (5¢-CGAATGCCGGCGCGGCTAAGC) ... glucose- 1-phosphatase and human prostatic-acid phosphatase. The polypeptide chain is organized into an a and an a ⁄ b domain, and the active site is located in a positively charged cleft between the domains. ... groups of HAPs are adapted to different habitats. To support plant growth, bacteria do not need to release phosphate as fast as the diges- tive tract of an animal host, where possible substrates might...
Ngày tải lên: 16/02/2014, 09:20
... include evaluation of audit reports and an assessment of the sources of risk assurance available to the Board and the audit committee. An evaluation of the level of assurance that has been obtained ... result of work by a team drawn from the main risk management organisations in the UK – the Association of Insurance and Risk Managers (AIRMIC), the public sector risk management association (Alarm) ... components that are key to success ● HAZOP and FMEA approaches Hazard and Operability studies and Failure Modes Effects Analysis are quantitative technical failure analysis techniques ● SWOT and PESTLE...
Ngày tải lên: 21/02/2014, 12:20
Báo cáo khoa học: Structure of a trypanosomatid mitochondrial cytochrome c with heme attached via only one thioether bond and implications for the substrate recognition requirements of heme lyase potx
... mature a bacterial class I c-type cytochrome, Paracoccus denitrif- icans cytochrome c 550 [33]. Moreover, many taxa that have heme lyase apparently have separate heme lyases for the maturation of ... compilation ª 2009 FEBS 2831 of the alanine of the AXXCH motif (Ala25) (in green) and the unsaturated vinyl group of the heme (cyan) are separated by 3.41 A ˚ (as compared with a typical thioether ... Trypanosomatidae and the glycosomal redox balance of insect stages of Trypanoso- ma brucei and Leishmania spp. Mol Biochem Parasitol 149, 155–169. 16 van Hellemond JJ, Simons B, Millenaar FF & Tielens...
Ngày tải lên: 23/03/2014, 04:21
Báo cáo khoa học: Bioenergetic requirements of a Tat-dependent substrate in the halophilic archaeon Haloarcula hispanica pdf
... (TTTGTTTAACTTTAAGAAGG AGATATA CATATGAATCG) and AmyRev-NcoI (aaaac catGGGCTTTGTTAGCAGCCGGAT). The amplified fragment was ligated into the NdeI and NcoI sites of pSY1 [30]. A derivative (pSY-AmyH_KK) was ... mutated to twin lysines (AmyH- KK). The primers used for Quickchange mutagenesis were AmyKKfor (CCGGCAGTAAGCAGGCGTCTaagaaaACC GTTCTGAAAGGAATCG) and AmyKKrev (GGCCGTC ATTCGTCCGCAGAttctttTGGCAAGACTTTCCTTAGC) (bold ... pET-AmyH for use in in vitro transcrip- tion ⁄ translation (see below), amyH was amplified using chromosomal DNA of H. hispanica as template, and prim- ers AmyH-T 7a (atat catATGAATCGACCCCGAATTACC GGCAG)...
Ngày tải lên: 23/03/2014, 06:20
Guidance for Determining Whether a Poultry Slaughter or Processing Operation is Exempt from Inspection Requirements of the Poultry Products Inspection Act docx
... Ext. 249 FAX: (510) 337-5080 Emergency: (202) 276-1610 Western Region States: Alaska, American Samoa, Arizona, California, Colorado, Guam, Hawaii, Idaho, Mariana Islands, Nevada, New Mexico, ... Basic Sanitary Standards 23 Attachment 3 FSIS District Office Information 25 Attachment 4 OPEER Regional Offices Contact Information 27 Attachment 5 Adulterated 28 Attachment 6 State Officials ... P.L. 90-492.” Instead of the Federal law, a State law may be cited when operations are exempted under the authority of a State law and the operations are reviewed by a State Agency. Producer/Grower...
Ngày tải lên: 23/03/2014, 21:20
Cambridge.University.Press.War.and.the.Law.of.Nations.A.General.History.Sep.2005.pdf
... the rational craftsman’s approach to war could easily work against ideas of restraint in war, a point dramatically illu- strated in India by the Arthas ´ astra of Kautilya. This was a manual of statesmanship ... formal declaration. 60 Nor, apparently, was it thought necessary to have a formal declaration of war in the case of certain small-scale, one-off operations of a punitive character. 61 Declarations ... implications for international law of such a sharp demarcation of the boundary between war and peace were immense. Most outstandingly, it made the idea of a state of war easy to envisage. In such a...
Ngày tải lên: 21/09/2012, 11:02
The effects of clay a m e n d m e n t and composting on metal speciation in digested sludge liang qiao
Ngày tải lên: 23/09/2012, 14:47
A study to indicate the importance of consumer based-brand equity on consumer perception of brand (a case study of fast food restaurants).pdf
... was good”. In total, MacDonald achieved a higher overall mean value than Max hamburger. In terms of standard deviation, we found out that MacDonald had a larger standard deviation than Max ... knowledge and awareness of the available brand. • Alternative evaluation: this is the stage whereby the consumers evaluate and rank alternative brand based on the information they have. Such information ... said to have a larger degree of dispersion of data around the mean than Max hamburger in those attributes. Max hamburger had a larger standard deviation than MacDonald in these attributes“serving...
Ngày tải lên: 24/09/2012, 17:19
Jist Works Inside Secrets Of Finding A Teaching Job
... bilingual education) ● Speech/Language Credential ● Special-Education Credential The availability of jobs in these areas often far exceeds the supply of creden- tialed candidates. And many of the ... that having a second credential or certificate makes a candidate more attractive to the school district. A credential or certificate in one of these three areas can greatly enhance your chances ... newspaper ads. You’ll be surprised how many up-to-date job vacancies are listed regularly, not only in your local paper, but nationally. What many teacher candidates don’t realize is that many national...
Ngày tải lên: 05/10/2012, 09:47
600 sentences of certificate A and B
... will Jean's cat while she is away? a. take care of b. take track of c. take advantage of d. get in touch with > a 223. I don't like your behavior. You are grown up but you are still ... > a 10. Our English lessons are long. a. many b. much c. a lot of d. very > d 11. Bangkok is capital of Thailand. a. a b. one c. the d. an > c 12. Are you free Saturday? a. on b. at c. ... no article > b 55. That is a bag. It is on table. a. the b. a c. an d. no article > a 56. We are in same class. a. the b. a c. an d. no article > a 57. Your book is the desk. a. at b....
Ngày tải lên: 05/11/2012, 09:18
Representations of Death A Social Psychological Perspective
... three representations of death were layered on top of one another. The apparently arbitrary character of the split between good and bad or natural and unnatural does not imply that they do not have an important ... hefty delays called baby Joe and baby Ellie and I was particularly thankful that I had such an understanding person in Heather Gibson. Thanks also to Fiona Bailey for being such a calm and helpful ... environment alone that has caused me to write the book afresh. The experiences of marriage, parenting, bereavement and psychoanalysis have changed me and have had an impact on the way in which I have...
Ngày tải lên: 07/11/2012, 14:20
Starch and Protein Quality Requirements of Japanese Alkaline Noodles (Ramen).pdf
... substantially affected by small levels of α-amylase, and inactivation by means of 1 mM AgNO 3 was a critical requirement in characterizing the quality of the starch component of flour. Alkaline ... Concentration on α-Amylase in RVA Tests The rain-damaged wheat sample had a FN of 209 sec. Inactivation of the α-amylase in this sample was essentially achieved at AgNO 3 concentrations of ≥0.5 mM (Fig. ... mixer fitted with a flat beater. A solution containing analytical-grade potassium carbonate (2.4 g), analytical-grade sodium carbonate (1.6 g), analytical-grade sodium chloride (4g), and suffi- cient...
Ngày tải lên: 16/12/2012, 15:25
The uses of article “a/an” Abstract
... 30miles a/ per gallon Frequency/time: Four times a day 3.10 A/ an after What and such (Alexander, L.G, 1988, P 37) A/ an used with countable nouns after What in exclamations Eg: What a shame! What ... classifying the nouns or noun phrases (p.43) Another definition of English articles is that articles are, in fact, limiting adjectives, but they are generally treated as a separate part of ... Nam in general and in many universities in particular. For many students learning English is vital need. To learn English well, grammar plays a very important role of which articles are a...
Ngày tải lên: 14/04/2013, 23:39
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