repopulating patterns of primitive hematopoietic stem cells

... mechanism responsible for induction of apoptosis and the relevant signaling pathways in hematopoietic stem cells are still poorly understood Hematopoietic stem cells (HSCs) contain specific markers ... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...

Ngày tải lên: 18/06/2014, 19:20

... mechanism responsible for induction of apoptosis and the relevant signaling pathways in hematopoietic stem cells are still poorly understood Hematopoietic stem cells (HSCs) contain specific markers ... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...

Ngày tải lên: 20/06/2014, 04:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and types of adult cells, HUVEC, ... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of...

Ngày tải lên: 18/06/2014, 15:20

... mobilization of cells expressing hematopoietic stem cell markers Data is presented as percentage of mononuclear cells in Additional File Analysis of the number of the progenitor cells in circulation ... feasibility of significant mobilization of cells expressing hematopoietic stem cell and EPC markers and properties The area of nutritional modulation of the stem cell compartment offers significant ... proliferation of human stem cells Stem Cells Dev 2006, 15(1):118-23 77 Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem...

Ngày tải lên: 18/06/2014, 16:20

... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... and m-FISH (Figure 3) of metaphase spreads Hence, the results of this study could have useful implications for the salvage of hESC culture during infrequent occurrences of incubator break-down...

Ngày tải lên: 31/10/2012, 16:57

... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA ... hybridizes just downstream of The commitment of pluripotent stem cells to successively less plastic progenitors and, finally, differentiated cells exhibiting stable expression patterns is thought to ... context of this system the locus remains so in a number of prehematopoietic precursor cell populations and undergoes a number of alterations in chromatin structure and factor recruitment as these cells...

Ngày tải lên: 07/03/2014, 12:20

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... mean fat cells size and fat cells area would be measured with imaging process software Image-Pro Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD) Fat cells density = marrow fat cells number...

Ngày tải lên: 20/06/2014, 00:20

... presence of putative stem cells The analysis confirmed the presence of round, SSEA-4 and SOX-2-positive primitive oocyte-like cells in the ovarian surface epithelium These cells resembled primitive ... epithelium of fetal ovaries (Figure 1) If not primitive oocytes, these cells might be oogonia or stem cells expressing markers of pluripotency They were round, morphologically resembled primitive ... Virant-Klun et al Journal of Ovarian Research 2011, 4:13 http://www.ovarianresearch.com/content/4/1/13 round cells with diameters of to μm that express some markers of pluripotent stem cells and can develop...

Ngày tải lên: 20/06/2014, 08:20

... hematopoiesis Thus, much of what we know about stem cells is imprecise and based on inferences from genetically manipulated animals Cardinal Functions of Hematopoietic Stem Cells All stem cell types have ... source of all blood and immune cells, maintaining a capacity to produce a broad range of cells from a single cell source and yet keeping itself vigorous over decades of life As an individual stem ... accomplish one of three division outcomes: two stem cells, two cells destined for differentiation, or one stem cell and one differentiating cell The former two outcomes are the result of symmetric...

Ngày tải lên: 07/07/2014, 01:20

... or a different host Mobility of Hematopoietic Stem Cells Cells entering and exiting the bone marrow so through a series of molecular interactions Circulating stem cells (through CD162 and CD44) ... cleavage of CXCL12, or downregulation of the receptor can all result in the release of stem cells into the circulation This process is an increasingly important aspect of recovering stem cells for ... The presence of stem cells in the circulation is not unique to a time window in development Rather, hematopoietic stem cells appear to circulate throughout life The time that cells spend freely...

Ngày tải lên: 07/07/2014, 01:20

... proliferation Hematopoietic stem cells have governing mechanisms that are distinct from the cells they generate Hematopoietic Stem Cell Differentiation Hematopoietic stem cells sit at the base of a branching ... location of stem cells by indirect means, altering molecules such as CXCL12 that tether stem cells to their niche Molecules shown to be important for altering the proliferation of stem cells, such ... a comprehensive listing of gene expression in stem cells) As stem cells mature to progenitors, precursors, and, finally, mature effector cells, they undergo a series of functional changes These...

Ngày tải lên: 07/07/2014, 01:20

... capacity, stem cells have an additional feature characterizing their proliferation machinery Stem cells in most mature adult tissues are deeply quiescent In the hematopoietic system, stem cells are ... effectively expand human hematopoietic stem cells The process may be controlled by particularly high levels of expression of cyclindependent kinase inhibitors that restrict entry of stem cells into cell ... molecular regulation of self-renewal For medicine, self-renewal is perhaps the most important function of stem cells because it is critical in regulating the number of stem cells Stem cell number...

Ngày tải lên: 07/07/2014, 01:20

... experimentally in the hematopoietic system Taking advantage of the cell-surface markers that distinguish hematopoietic cells of varying maturity, stem cells, progenitors, precursors, and mature cells can ... That aspect of medicine will include the use of the stem cells and the use of drugs to target stem cells to enhance repair of damaged tissues It will also include the careful balance of interventions ... HoxB4 is capable of inducing extensive self-renewal of stem cells through its DNA-binding motif Other members of the hox family of genes have been noted to affect normal stem cells, but they are...

Ngày tải lên: 07/07/2014, 01:20

... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult-derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the ... progenitor or stem cells locally would represent a strategy to promote regeneration of the diseased and injured nervous system Alternatively, new neuronal cells are generated at sites of degeneration...

Ngày tải lên: 08/08/2014, 17:20

... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent ... involvement of Wnts is complicated by their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of ... is the homing of stem cells to sites of injury and subsequent wound healing Although some tissue repair may be accomplished by the division of indigenous differentiated cells, such cells are most...

Ngày tải lên: 09/08/2014, 10:20

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... suppression of chordin led to an increase in the bioavailability of endogenously produced BMP-2 to drive the differentiation of Page of (page number not for citation purposes) Effect of chordin ... extrapolated to cells from a large population of donors because there is consistently a significant and large increase in expression of osteogenic markers, as a result of chordin knockdown in cells from...

Ngày tải lên: 09/08/2014, 10:23

... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8 ... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... cartilage, and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the...

Ngày tải lên: 09/08/2014, 14:22

... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells [18] ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells...

Ngày tải lên: 10/08/2014, 05:21

... basal/ stem cells Patch1 and Smo were found highly expressed in the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of ... biology of the prostate: stem cell biology Urology 2003, 62:11-20 Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate ... and stem cell characteristics of the transformed P63+ basal /stem cells, supporting that they could be the origin of PCSCs under the influence of persistent Hedgehog overexpression P63+ basal/stem...

Ngày tải lên: 10/08/2014, 05:21