renewal pluripotency and tumorigenesis in pluripotent stem cells revisited

... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... of epigenetic patterns in defining cell identity, understanding and utilizing epigenomic mapping will become a necessity in both basic and translational stem cell research In this review, we strive ... Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36 ...

Ngày tải lên: 11/08/2014, 12:21

... lower in group than in groups 3, 4, and 5, significantly lower in group than in groups and 5, and also notably lower in group than in Page 10 of 16 group This finding indicates that early combined ... stained cells in LV myocardium were significantly higher in group than in other groups, significantly higher in groups and than in groups and 5, and also notably higher in group than in group Lin ... groups and These findings indicate that the expression of cytochrome C, an index of energy supply and storage in mitochondria, was notably lower in group than in groups and The increase in cytosolic...

Ngày tải lên: 18/06/2014, 16:20

... Medicine 2011, 9:12 http://www.translational-medicine.com/content/9/1/12 Figure Intraoperative view showing clinical application of stem cells into the failing heart with multiple targeted injections ... Conception and design, provision of patients, data analysis and interpretation, manuscript writing PA Conception and design, data analysis and interpretation, manuscript writing HA Data analysis and interpretation ... Conception and design, data analysis and interpretation, manuscript writing All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests...

Ngày tải lên: 18/06/2014, 16:20

... and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected into tissue explants...

Ngày tải lên: 09/08/2014, 01:22

... observed in more recent GWASs [23] Linking GWAS findings to AD pathogenesis Both APOE and CLU are lipoproteins that are found in the brain, with APOE being the predominant brain lipo­ protein (reviewed ... lines and patients By comparing iPSC-derived neurons and/ or glia between individuals, it may be possible to validate findings from GWAS and animal models studies and to identify novel initiating ... disease and to investigate disease phenotypes in vitro [31,32] iPSCs are most commonly made by taking a small skin biopsy from a patient, expanding the biopsy into primary fibroblasts, and transducing...

Ngày tải lên: 11/08/2014, 12:21

... embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent stem cell; lincRNA, long-intergenic non-coding RNA; lncRNA, long non-coding RNA; mESC, mouse embryonic stem ... other non-coding RNA, such as long intergenic non-coding RNA (lincRNA) Conversely, Oct4, Sox2 and Nanog, in concert with Polycomb group proteins (PcG), bind lineage-specific and non-coding RNA genes, ... decrease in both Wdr5 binding and H3K4me3 levels at Oct4-Wdr5 co-bound promoters This indicates that Oct4 may be responsible for directing Wdr5 to ESC genes and main­ taining H3K4me3 open chromatin...

Ngày tải lên: 11/08/2014, 12:21

... Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington DC; 1981 Landry DW, ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics J...

Ngày tải lên: 13/08/2014, 23:20

... which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) ... iPSCs and this reprogramming quartet became the first non-OSKM reprogramming combination reported in the iPSC field It is interesting to note that Nanog was included in Kazutoshi Takahashi and Shinya ... implicated in maintaining the pluripotency or self -renewal of ESCs Strikingly, our group subsequently reported that 15 Klf4 and its close family members, Klf2 and Klf5 are important for the self-renewal...

Ngày tải lên: 09/09/2015, 18:56

... musashi and prominin-1 (CD133) Nestin is an intermediate filament protein expressed in neuroepithelial cells (Lendahl et al., 1990) and is found mainly in proliferating cells of the CNS during early ... proteoglycan Inner cell mass Iduronic acid IGF binding protein Inducible pluripotent stem cells Janus kinase Keratan sulfate Leukemia inducing factor Lipoprotein receptor related protein Mitogen ... maintain these cells in an undifferentiated, quiescent state For stem cells to be activated, the niche environment must change (Fuchs, 2007) 1.4.3 Stem cells and cancer Stem cells and cancer cells...

Ngày tải lên: 12/09/2015, 21:26

... of cells transitioning from fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent ... generate porcine induced pluripotent stem cells (IPSCs) from porcine fibroblast cells and differentiate such cells into cardiac lineages to provide a model to study the development of cardiac cells ... for the survival and the maintenance of its pluripotency( Brevini, Antonini, Cillo, Crestan, & Gandolfi, 2007) 3.3 Generation of Induced Pluripotent Stem Cells Embryonic stem cells (ESCs) hold...

Ngày tải lên: 30/09/2015, 10:13

... human lines using basically the same culturing techniques M Amit and J Itskovitz-Eldor, Atlas of Human Pluripotent Stem Cells: Derivation and Culturing, Stem Cell Biology and Regenerative Medicine, ... antibodies into the blastocyst, thus leaving the ICM cells intact Incubation with guinea pig complement-containing medium then lyses all antibodymarked cells The intact ICM is further rinsed and cultured ... discussed in another chapter describing the common principles of karyotyping and fluorescent in situ hybridization (FISH) methods as they apply to the field of pluripotent stem cells Induced pluripotent...

Ngày tải lên: 22/03/2014, 09:20

... might induce MAP kinase activation independently of ouabain Ouabain was added to the cells at various concentrations in ECGM-SF, and the cells were further incubated at 37 °C for 30 The cells ... counting The 86Rb+ activity was normalized against the amount of protein [13] per well 86 Rb+ uptake was also investigated after preincubating the cells for 15 with lM protein kinase C inhibitor ... h For counting, cells were detached and dispersed in trypsin/ EDTA (Gibco) The trypsin was then neutralized using : M199/ECGM with 50% fetal bovine serum and placed on ice Suspended cells were...

Ngày tải lên: 30/03/2014, 13:20

... chymotrypsin and trypsin, including proteinase K, thermolysin, endoproteinase lys-C, Cat L, Cat B and Cat S[3,19-21] Recent work has provided insight into the cellular determinants of reovirus uncoating ... lower in U937 cells than in L929 cells This may be a consequence of high expression in U937 cells of cystatin F, an intracellular cysteine protease inhibitor with specificity for Cat L and papain ... interacting with, protecting, and controlling the conformational status of the underlying penetration protein µ1 [15-18] In cells that cannot efficiently mediate σ3 degradation during uncoating,...

Ngày tải lên: 19/06/2014, 08:20

... chymotrypsin and trypsin, including proteinase K, thermolysin, endoproteinase lys-C, Cat L, Cat B and Cat S[3,19-21] Recent work has provided insight into the cellular determinants of reovirus uncoating ... lower in U937 cells than in L929 cells This may be a consequence of high expression in U937 cells of cystatin F, an intracellular cysteine protease inhibitor with specificity for Cat L and papain ... interacting with, protecting, and controlling the conformational status of the underlying penetration protein µ1 [15-18] In cells that cannot efficiently mediate σ3 degradation during uncoating,...

Ngày tải lên: 20/06/2014, 04:20

... tissues were finely minced and digested with 0.2% collagenase in DMEM containing 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin (complete DMEM) Following overnight incubation ... shown) Inversely, the mean induction of osteocalcin and alkaline phosphatase was statistically higher in cells from BM than in cells from the synovium In these conditions, the primary skin fibroblastic ... environment inside the joint favoring the inhibition of immune cell proliferation In the inflammatory context, where TNF-α and IFN-γ are prominent, induction of proinflammatory cytokines may reverse...

Ngày tải lên: 09/08/2014, 07:20

... Holtmann and colleagues found that introduction of an activating mutation in MKK6, increased p38 and, stabilized IL-8 mRNA and further increased IL-8 protein formation induced by NFκB-inducing kinase ... with increased and persistent activation of IKK and NFκB in the AS cells as compared to S cells [2, 14] In this study, we determined the expression of IL-8 mRNA in AS/S and IB3-1/S9 cell lines in ... [22] Cells were maintained in a 5% CO2 incubator at 37°C using MEM (Mediatech, Inc Herndon, VA) for AS and S cell lines and LHC-8 media (Biosource, Camarillo, CA) for IB3-1 and S9 cell lines...

Ngày tải lên: 11/08/2014, 03:20

... contaminating germ cells The Cortactin immunostaining Sertoli cells were fixed with 100% ethanol and immunostained for cortactin, as described by Wine and Chapin [25], using 10 µg/ml anti-cortactin ... determine if cortactin is involved in the phagocytic process of the Sertoli cell, as indicated in HeLa cells [17], in which positive cortactin immunostaining was observed in association with actin ... BioMedicine 2005, 4:11 The observations of Dehio et al [17] that cortactin appears to be involved in early phagocytosis in HeLa cells suggested that this actin binding protein may also be involved in...

Ngày tải lên: 11/08/2014, 08:20

... transgenes flanked by inverted terminal repeats [8,9] The PB system is effective in mouse and human cells, and performs efficient and precise excision without leaving a footprint behind [17,18] Woltjen ... reprogramming factors are sufficient in single copy for reprogramming The transient expression of PB transposase in the two singlecopy cell lines and subsequent subcloning resulted in the removal ... Ding S: A com bined chemical and genetic approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction...

Ngày tải lên: 14/08/2014, 21:20