removal of heme from red blood cells

... thousands of merozoites Upon the rupture of these exoerythrocytic schizonts, the released merozoites flow into the blood stream to infect red blood cells Parasite invasion of red blood cells (RBCs) ... OF MALARIA INFECTED RED BLOOD CELLS LI ANG (B S., FUDAN UNIVERSITY, CHINA) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MACHANICAL ENGINEERING NATIONAL UNIVERSITY OF ... 2% of the total blood volume Whereas P malariae only infects older red cells with the number even more limited The unique sequestration or cytoadherence property of P falciparum infected red cells...

Ngày tải lên: 11/09/2015, 14:34

... (Table 1) Table The purity of human RBCs fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* ... Analysis of total human RNA of RBCs (Bioanalyzer 2100 Agilent, USA): A) L- RNA 6000 ladder (Ambion, USA); 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of total ... Rassaf T et al Red blood cells express a functional endothelial nitric oxide synthase Blood 2006;107:2943-2951 19 Newman PJ, Gorski J, White GC2 et al Enzymatic amplification of platelet-specific...

Ngày tải lên: 03/11/2012, 10:58

... Ions from m ⁄ z 480–2000 were selected MALDI-TOF/TOF In addition to LC-MS ⁄ MS analysis, we also completed TOF ⁄ TOF analysis of selected peaks to confirm structure using a MDS Sciex 4800 MALDI-TOF ... measurement of various concentrations of solutions can then be used to quantify viral titer Additionally, the introduction of antisera capable of neutralizing a viral strain reduces the ability of virus ... overall structural fingerprint of the sample MALDI-MS analysis of released N-glycans from cRBCs indicates the presence of a wide range of structures; tentative assignments of molecular ions are reported...

Ngày tải lên: 14/02/2014, 19:20

... Bickle2 Laboratory of Dr Ettrich, Institute of Physical Biology of USB and Institute of Landscape Ecology of AS CR, Nove Hrady, Czech Republic, 2Laboratory of Dr Bickle, Department of Microbiology, ... elevation of total antioxidant capacity of plasma leading to suppression in the level of lipid peroxidation products in plasma V1-006P Functional analysis of peptides from the skin extracts of the ... catalysis of NO oxidation can maintain uninterrupted fluxes of NO and oxygen to the interior of the protein molecule, formation of RNS inside the molecule, and transfer of the reaction products out of...

Ngày tải lên: 30/03/2014, 20:20

... in-hospital mortality of NRBC-positive patients of this Figure medical intensive care patients blood cells (NRBCs) in the blood of Concentration of nucleated red who have died from various causes ... measured on the day of the first appearance of nucleated red blood cells in blood n.s., not significant APACHE II, Acute Physiology and Chronic Health Evaluation II; NRBC, nucleated red blood ... Incidence of nucleated red blood cells in the blood of hospitalised patients Infusion Ther Transfus Med 2001, 28:263-266 Stachon A, Sondermann N, Imöhl M, Krieg M: Nucleated red blood cells indicate...

Ngày tải lên: 13/08/2014, 03:21

... of Figure Predicted risk of death against maximum age of red blood cells A locally weighted nonparametric smoother (LOWESS) for the predicted probability of death and the maximum age of red blood ... to deformability of stored red cells Br J Haematol 1983, 53:237-240 Card RT, Mohandas N, Perkins HA, Shohet SB: Deformability of stored red blood cells Relationship to degree of packing Transfusion ... of the length of storage of transfused red cells Transfusion 1999, 39:701-710 Offner PJ, Moore EE, Biffl WL, Johnson JL, Silliman CC: Increased rate of infection associated with transfusion of...

Ngày tải lên: 14/08/2014, 08:21

... considered to be important to parasite multiplication inside the human body, and the altered mechanical properties of infected red blood cells (iRBCs) are considered to be the main cause of malaria ... detached from the CHO cell are compared between cells measured at 23°C, 37°C and after h incubation at 40°C 91 The adhesion energy density of cell-cell adhesion measured between the iRBCs and CHO cells ... photobleaching GAG sulfated glycosaminoglycan HSP heat shock protein nRBCs normal red blood cells iRBCs infected red blood cells KAHRP knob associated histidine rich protein PAM pregnancy-associated...

Ngày tải lên: 09/09/2015, 18:52

... List of Figures v List of Tables .viii Abbreviations and Notations ix Summary x Chapter Introduction 1.1 Red Blood Cell Membrane 1.2 Micro-rheology of Red Blood ... erythrocytes from patients with hereditary sperocytosis revealing the abnormal surface pseudopodia These cells become normal on removal of the spleen The peripheral blood plasma of uremic patients ... modulus of points A, B, C, D, and E of a swollen RBC in PBS solution 59 Figure 28 Cross section profile of a fresh RBC in PBS solution 60 Figure 29 Force curves of A, B, C, D, and E of a fresh...

Ngày tải lên: 30/09/2015, 14:23

... and old red blood cells 100 4.5.5 Phosphatidylserine exposure of young and old red blood cells 101 4.5.6 Phosphatidylserine exposure of stored red blood cells 103 4.5.7 Membrane redox ... and old red blood cells 23 2.5.2 Ca2+ content in young and old red blood cells 24 2.5.3 Influence of ageing on membrane redox system in red blood cells 25 2.5.4 Relevance of ageing ... redox activity of young and old red blood cells 105 4.5.8 Surface structure of young and old red blood cells 105 Discussion 107 5.1 Role of Ca2+ in red blood cells under physiological...

Ngày tải lên: 15/11/2015, 12:54

... similar case of surgical removal of lipoma from an area with tattooed skin using this aesthetic procedure was found in literature The intent of the case study is to highlight the use of pre-existing ... to meet the need of our patient of removing the lipomatous formation while keeping the tattoo intact, during the surgical treatment of the lipoma, we followed the exact lines of the tattoo itself ... consent was obtained from the patient for publication of this case report and accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal AUTHORS'...

Ngày tải lên: 25/10/2012, 11:35

... stock solutions of As(III) were prepared from As(III) standard solution of 1003mg/L (Wako Pure Chemicals Ltd, Japan) The stock solutions of As(V) were prepared by dissolving 4.1646mg of Na2HAsO4.7H2O ... 70.19m²/g, pore volume of 0.00062cm3/g and pore size of 126Å Preparation of AC-Fe AC from coconut shell was taken from MAPECOH, Inc (Philippines) with a particle size of 0.45-0.71mm after sieving ... aims to study the adsorption column for removal of arsenic from synthetic groundwater using sequential combination of LA and AC-Fe as adsorbents The effect of LA/AC-Fe ratio, flow rate, initial...

Ngày tải lên: 05/09/2013, 09:38

... prepared from an available, cheap source of carbon (rice husk carbon) and the most effective agent (iron) for the removal of both As[III] and As[V] from simulated groundwater using a mixture of ... investigated the usability of untreated rice husk packed in glass columns (2 cm x 30 cm) for the removal of As[III] and As[V] Lee et al (1999) reported the removal of 80% arsenate from highly concentrated ... investigate the effects of pH values within the range of 5-9, of initial arsenic concentrations within the range of 1.42 - 7.39 mg/L and of adsorbent doses within the range of 0.5 - 5.0g/L, as well...

Ngày tải lên: 05/09/2013, 09:38

... concentration of cadmium was 10 mg-Cd/L, the volume of solution was 50 mL, and the amount of charcoal was 20 mg Three days from the start of contact (concentration of cadmium was mg-Cd/L, amount of cadmium ... in charcoals prepared under 400°C and 500°C Such adsorbent can be applied for the adsorption of large molecules, and it is one of the important features of charcoals prepared from giant reed With ... 1.1 N 0.5 Ash 3.1 Journal of Water and Environment Technology, Vol 8, No.4, 2010 Removal of cadmium from aqueous solutions For estimating the removal rate, time courses of cadmium concentration...

Ngày tải lên: 05/09/2013, 10:15

... supernatants from uninfected Jurkat (gift of Kendall Smith, Cornell University, Ithaca, NY) and SupT1/CCR5 (gift of James Hoxie, University of Pennsylvania, Philadelphia, PA [17]) cells, two cell ... signal from the captured CD45 Actin is an abundant component of vesicles and can be used as a marker for vesicular contamination in the absence of virus [21] To assay for the presence of this ... from the SupT1/CCR5 cells contained a large amount of cellular proteins compared with the Jurkat E61 preparation (Figure 1B) CD45 immunoaffinity depletion markedly removed the contamination from...

Ngày tải lên: 13/08/2014, 05:21

... expression of Vif from pNL-A1 only moderately reduced the steady-state levels of APOBEC3G in HeLa cells Quantitation of the data confirmed that expression of APOBEC3G in the presence of Vif was reduced ... access of antibodies used in the experiments Thus, the discrepancy between our findings of the coexpression of Vif and APOBEC3G in the majority of cells and the virtual exclusion of APOBEC3G from ... experiment in some cells both in the cytoplasm and the nuclei of cells (red arrow heads) Finally, we also observed cells expressing Vif that were APOBEC3G-negative (blue arrow heads) Overlay of the Vif...

Ngày tải lên: 13/08/2014, 13:20

... solution of 5000 mg L−1 phenol was prepared by dissolving 5.00 g of phenol (Merck, Darmstadt, Germany) in L of deionized water The required concentration of phenol solutions were prepared by diluting ... distribution of active sites on the surface of the organobentonite 3.5 Effect of organobentonite concentration The effects of organobentonite concentration on the removal of phenol from aqueous ... Sivanesan, Removal of acid Violet 17 from aqueous solutions by adsorption onto activated carbon prepared from sunflower seed hull, J Hazard Mater 151 (2008) 316–322 [33] P.S Nayak, B.K Singh, Removal of...

Ngày tải lên: 01/12/2016, 16:24

... Namasivayam of dairy (11) and coworkers from employed solutions of using containing congo fly ash and coal(g) et al (12) have of nickel of this a textile such of chlorophenol study was to evaluate red ... 25.7 % as dye removal indicating time and red by red mud initial with stages lapse of of time 90 uptake for all the dye of dye decreased concentrations curves monolayer increased from were single, ... A Effect of initial pH on per cent removal of dye B Effect of initial pH on Final pH C Per cent removal vs Final pH In acid medium, positive charge develops on the surface of oxides of adsorbent...

Ngày tải lên: 23/09/2012, 14:47

... GM1, from lipid extracts of both differentiated cells The ability of LT-I to interact more efficiently with the complex glycosphingolipids carrying the blood group A determinant from polarized cells ... cells Results Characterization of differentiated HT29 cells In order to analyze the interaction of LT-I with cells that resemble the polarized enterocyte, HT29 cells from American type culture collection ... target of LT-I is a polarized intestinal cell, the purpose of this study was to investigate the interaction of LT-I with non-GM1 receptors of polarized HT29 cells Toxic activity, triggered by...

Ngày tải lên: 30/03/2014, 10:20

... cells In blood samples, found to be negative for presence of EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38% (14/37) of peripheral blood samples, and the median of the number of these ... presence of EpCAM-CK+ cells in samples (2/15 = 13%) when cells were enriched by CD45 depletion The median number of CK+ cells was 2/ 10 mL blood with an overall false positive rate

Ngày tải lên: 18/06/2014, 19:20

... = 1) is shown from Suppression Assays of CD33+ or CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture ... blood cells are analyzed by routine flow cytometry for the presence of myeloid-derived suppressor cells (MDSC) as a marker for tumor presence Active MDSC are distinguished from normal blood cells ... characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines Journal of Translational...

Ngày tải lên: 18/06/2014, 19:20