relevance of host factors in gastric cancer associated with helicobacter pylori

... staining of D2-40 is similar to that of the COX-2 staining at a dilution of 1:100 Evaluation of immunohistochemical staining Fifty-six patients with histologically proven gastric adenocarcinoma ... staining of Cox-2 in the gastric carcinoma: the positive expression of COX-2 protein was stained as yellow or brownish yellow in the cytoplasm of carcinoma cells (LsAB, ×400) The correlation of ... combining the quantity score (percentage of positive stained cells) with the staining intensity score The quantity score ranges from to 4, i.e 0, no immunostaining; 1, 1-10% of cells are stained;...

Ngày tải lên: 10/08/2014, 10:21

... system for screening of interacting partners inside human cells using the linker histone as a bait protein This novel interaction screening method could potentially be used with any of the numerous ... Box is the binding site for the TATA-binding protein (TBP) In some genes, the transcription initiation site consists of the initiator element (Inr) defined as an element encompassing the transcription ... showing in vivo Interaction 90 between linker and core histones hH3 and hH4 3.4 Co-immunoprecipitation showing binding of linker 91 histone with core histones hH3 and hH4 3.5 Molecular modeling...

Ngày tải lên: 29/09/2015, 13:01

... emission wavelength of 575 nm (A) Fluorescence intensity analysis of intracellular adriamycin in gastric cancer cells (B) Adriamycin releasing index of gastric cancer cells The releasing index = (accumulation ... siRNA of Akt Fig Fluorescence intensity and releasing index of adriamycin in gastric cancer cells Gastric cancer cells were treated with or without LY294002 (10, 20 or 40 lM) or co-transfected with ... above in vivo findings PI3K/Akt is involved in PrPC-mediated MDR in gastric cancer In order to study whether activation of the PI3K/Akt signaling pathway played a role in PrPC-induced MDR in gastric...

Ngày tải lên: 07/03/2014, 03:20

... grams of protein per kilogram of weight per day, by using an infusion bomb during 20 hours, (with a resting time of hours) Results The complete closing of the fistula was evident in day 8, 14 ... factors in gastric carcinoma: results of the German Gastric Carcinoma Study 1992 Cancer 1993, 72:2089-2097 Budisin N, Budisin E, Golubovic A: Early complications following total gastrectomy for gastric ... the adequate place, in the intestinal lumen, distal to the zone of escape (Figure 4) This emphasizes the importance of experience in the handling of this alternative therapy In this case, a radiographic...

Ngày tải lên: 09/08/2014, 03:22

... in distinguishing T1 and T2 lesions The cancer was found in the ileocecal junction of 11 patients (11.9%), in ascending colon of 14 patients (15.2%), in transverse colon of subjects (8.7%), in ... but the results of the previous studies were often intriguing and conflicting [4,5] Actually, most studies investigating prognostic factors for large bowel cancers did not distinguish between ... 0.01 The site of the tumor is one of the prognostic factors investigated Patients with colon cancer are considered having a better survival than those with rectal cancer [6,7,9] In previous studies...

Ngày tải lên: 09/08/2014, 07:21

... staining of STC-1 in tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice PCNA was detected in the ... gastric carcinomas of Chinese patients Cell Mol Immunol 2007, 4(2):135-140 Page of 13 Wang Q, Huang Y, Ni Y, Wang H, Hou Y: siRNA targeting midkine inhibits gastric cancer cells growth and induces ... fixed in 10% buffered formalin, dehydrated, bisected, mounted in paraffin, and sectioned for immunohistochemistry (IHC) Hydrated sections were stained using Hematoxylin/Eosin IHC was carried out with...

Ngày tải lên: 10/08/2014, 05:21

... acetylation of histone and tubulin in a scirrhous gastric cancer cell line J Exp Clin Cancer Res 2010, 29:149 [30] Japanese Gastric Cancer Association Japanese classification of gastric pg 23 carcinoma ... determine the expression of AdipoR1/R2 in gastric cancer cell lines, western blotting analysis was performed As shown in figure 1A, AdipoR1/R2 were positively detected in cell lines, and compared with ... H&E staining according to the Japanese Classification of Gastric Carcinoma [30] A fasting morning blood sample was obtained for the adiponectin assay from each patient after admission into the...

Ngày tải lên: 10/08/2014, 10:21

... lesion with a macroscopically clear margin of mm at the closest point Microscopically, the nodule was predominantly composed of oncocytes interspersed with a smaller proportion of clear cells with ... recently-described FLCNinteracting protein (FNIP1), FLCN may function in pathways signaling through the mammalian target of rapamycin (mTOR) Such involvement with mTOR signaling is a feature of several hamartoma ... Hopkins RF, Hartley JL, Furihata M, Oishi S, Wei Z, Burke TR Jr, Linehan WM, Schmidt LS, Zbar M: Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved...

Ngày tải lên: 11/08/2014, 12:20

... observed in CF remains incompletely explained and most likely reflects the influence of multiple, interrelated factors These may include differences in CFTR mutation and presence of infective ... to obtain DNA from premature infants, recipients of previous blood transfusions and infants with meconium ileus Mutation screening of the entire coding sequences of the CFTR gene (including the ... having increased VTG also demonstrated increased values for LCI Rate of progression of functional abnormality was most rapid within a subgroup of patients within whom both pulmonary hyperinflation...

Ngày tải lên: 12/08/2014, 16:20

... high association of Helicobacter pylori infection (Parkin, 2006) In Singapore, even though there is a declining trend in gastric cancer, it remains the 7th most frequent cancer in males and 8th ... gastric carcinogenesis , genes mainly involved in the progression of intestinal GC; , genes involved in both intestinal and diffuse GC; , genes mainly involved in diffuse GC ↑ indicates overexpression ... receptor of the tyrosine kinase family With IHC and FISH techniques, it is found that HER2 is over-expressed in approximately 20% of gastric cancer, especially in intestinal type gastric carcinoma...

Ngày tải lên: 09/09/2015, 08:13

... cycle of coronavirus is initiated with the binding of S protein to the host cell surface receptors The receptor specificity of S protein is therefore the key determinant for the virus’ host specificity ... functions in coronavirus replication 1.2.1.2 Polypyrimidine-tract binding (PTB) protein Polypyrimidine-tract binding (PTB) protein binds to the UCUAA pentanucleotide repeats encompassed within the ... systems involving viral RNA and replicase remains the choice approach for identifying cellular proteins to be involved in coronavirus infection, an increasing number of cellular proteins have...

Ngày tải lên: 09/09/2015, 10:07

... and institutional funding from Duke-NUS and Cancer Sciences Institute of Singapore I wish to acknowledge Department of Physiology, Yong Loo Lin School of Medicine in National University of Singapore, ... causes fusion of the BCR signaling protein to the ABL non-receptor tyrosine kinase, resulting in 20 constitutive tyrosine kinase activity and complex interactions of this fusion protein with many ... gene brings together the 5‘ part of BCR gene at 22q11 and the 3‘ part of the ABL1 tyrosine kinase-encoding gene at 9q34, leading to a hybrid BCR-ABL1 protein with increased tyrosine kinase activity...

Ngày tải lên: 09/09/2015, 18:49

... Reduction of 17 Gastric Cancer Cell Lines Induced by TSA………… 51 v 3.1.3 Growth Inhibition of 17 Gastric Cancer Cell Lines Induced by TSA………53 3.1.4 Apoptosis of 10 Gastric Cancer Cell Lines Induced ... Sensitivity of Gastric Cancer Cell Lines to entinostat (MS275)…… ……65 3.3.1 Growth Inhibition of Gastric Cancer Cell Lines Induced by MS275.…65 3.3.2 Apoptosis of Gastric Cancer Cell Lines Induced ... Sensitivity of Gastric Cancer Cell Lines to HDAC inhibitors………………….…….49 3.1 Sensitivity of Gastric Cancer Cell Lines to Trichostatin A (TSA)……….………49 3.1.1 Selection of Gastric Cancer Cell lines Experimental...

Ngày tải lên: 10/09/2015, 09:06

... GC Individuals with both HP infection and atrophic gastritis as measured by PG index had 14-fold increased risk of GC LIST OF TABLES Table 1.1 Incidence of Gastric Cancer in Singapore among Chinese, ... Trends in Incidence of gastric cancer in selected countries Figure 1.2 Age-specific Incidence of stomach cancer in the world and 13 in Eastern Asia Figure 1.3 Estimated Gastric Cancer Incidence ... occurs in East Asia Gastric cancer is also the second leading cause of cancer death worldwide accounting for 736,000 deaths in 2008 The known risk factors for gastric cancer are Helicobacter pylori...

Ngày tải lên: 10/09/2015, 09:29

... aberrant expression of a3b1 integrin in tumor cells and their malignant behavior The increased expression of a3b1 integrin in gastric carcinoma cells is associated with their increased invasion and ... factors including Ets, GATA, and MyoD/E-box binding factors The introduction of mutation into one of the putative Etsbinding sequences suppressed the promoter activity In addition, the specific binding ... deficient in this integrin receptor die during the neonatal period with kidney and lung defects and skin blistering [11] Additional abnormalities in the morphogenesis of limbs were observed in integrin...

Ngày tải lên: 21/02/2014, 03:20

... UCP2 modulates MDP-induced mitochondrial inefficiency central nervous system, and the lack of induction of in ammatory responses [22] Despite a long-standing interest in the field of muramyl peptides, ... findings agree with the hypothesis [29] that an increase in the mitochondrial membrane potential would slow the transport of electrons through the respiratory chain, increasing the time of interaction ... culture tube containing lL of FITC Annexin V and lL propidium iodide The cells were gently mixed and incubated for 15 at room temperature (25 °C) in the dark Finally, 400 lL of 1· binding buffer was...

Ngày tải lên: 05/03/2014, 23:20

... recognize specifically Ets motifs flanking the +1 site (A) The binding of nuclear factors is eliminated by mutations in Ets motifs The binding of nuclear factors present in SK-N-SH (K) or SH-SY5Y (H) ... contains two DNA binding inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory domain is poorly conserved and virtually specific to ERM, and the C-terminal domain that ... described above and binding reactions were carried out by incubating 0.1–0.2 ng of probe with lg of nuclear extracts in the presence of lg of poly(dI-dC)Æpoly(dI-dC) 18 in 10 mM Hepes pH 7.9,...

Ngày tải lên: 16/03/2014, 18:20

... application of vitamin D as a novel molecular target in gastric cancer therapies in association with the use of TSA ⁄ NaBu and 5-Aza Results Vitamin D induced apoptosis in gastric cancer cells ... chromatin structure to facilitate the binding of major 994 transcription factors on the promoter We were therefore interested in determining whether TSA ⁄ NaBu had any effects on promoting the binding ... advantage of the use of low concentrations of vitamin D and HDAC inhibitors to minimize their toxicities, owing to their remarkable synergistic effects in activating target genes An interesting finding...

Ngày tải lên: 22/03/2014, 21:20

... time points (or all of the time points for donor A) within the respective individual but were not seen in any other individual Overall, despite differences in HIV-1 genetic variability in each ... participates in the binding of Tat to TAR and is involved in the nuclear localisation of Tat [18,19] The strong or total suppression of transactivation abilities observed in many of the recipient ... of the cohort, there was considerable stability in the quality of mutations over time in each individual The transactivation abilities of each individual's unique Tat clones were assessed using...

Ngày tải lên: 18/06/2014, 18:20

... needed to maintain a high GC content [33] Silencing of specific repair enzymes in S typhimurium strains increases the mutation rate 6-fold to 100-fold with 98% of the mutations converting GC to ... bias detected in genomes of high GC content decreases the risk of acquiring nonsense mutations (Pearson’s r = -0.95; P < 0.0001) In the genomes of all examined vertebrates, including humans, this ... organisms with AT rich genomes have been explained by the lack of these repair enzymes [34] Despite knowing several mechanisms to increase and maintain a high GC content in a genome, the utility of...

Ngày tải lên: 18/06/2014, 19:20