... herbage yield (15.76 t/ha), shade dried herbage yield (11.90 t/ha) and essential oil yield (95.44 kg/ha) while minimum fresh herbage yield (9.13 t/ha), shade dried herbage yield (8.23 t/ha) and essential ... (2013) and Nilofer et al., (2015), where cv Kosi was superior in terms of yield and quality attributes.The effect of interaction between cultivars and planting date found nonsignificant on yield and ... Chandranath, H T., Pujari, B T and Halepyati., 2010, Influence of planting dates and stage of harvesting on root yield, root yield parameters and quality of ashwagandha Karnataka J Agric Sci.,...
Ngày tải lên: 09/01/2020, 14:47
... diameter, grain yield per plant, fresh stover yield per plant, dry matter yield per plant and grain harvest index and captured 26.85 per cent of total variation PC2 was represented by ear diameter and ... plant, head yield per plant (g plant-1), grain yield per plant (g plant-1), fresh stover yield per plant (g plant-1), dry matter yield per plant (g plant-1), 1000 -grain weight (g) and grain harvest ... plant and grain harvest index PC2 separated the material based on ear diameter and dry matter yield per plant On the basis of head yield per plant and 1000 grain weight, PC3 separated the lines and...
Ngày tải lên: 14/10/2020, 17:40
Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures 1
... lower recombinant protein yield and affects protein quality. It is hypothesized that extension of culture life can potentially improve recombinant glycoprotein yield and quality. Using an ‘in-house’ ... to Andrew Wu, Wei Jan, Wong Ju Wei, Nick Lim, Zeon Na, Amanda Lanza, Ang Pei Ling, Emily Lau and Dennis Goh for their help and enthusiasm! Last but not least, my family for being so understanding ... oligosaccharide standards and their... Signaling during BC and FBC (A) Up-regulation of Bim and Bad in the Mitochondrial-mediated 11 7 Apoptosis Signaling during BC and FBC (A) Down-regulation...
Ngày tải lên: 15/09/2015, 17:10
Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures 6c
... high cell densities at high viability and often leads to lower protein yields and quality (Arden and Betenbaugh, 2004; Fussenegger and Bailey, 1998; Laken and Leonard, 2001; Vives et al., 2003a). ... interferon gamma (IFN-g) were constructed and compared with the parental CHO in BC and FBC in terms of culture viability, IFN-g yields, and quality. MATERIALS AND METHODS Construction of Plasmids ... it was upregulated only in BC and not FBC. Overexpression of Bcl-2 and Bcl-xL has been reported to suppress the pro-apoptotic activities of Bim, Bak, Bax, and Bad, and as such these genes were...
Ngày tải lên: 15/09/2015, 17:10
Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures b
... often limits recombinant protein production and increases risk of product degradation (Arden and Betenbaugh, 2004; Fussenegger and Bailey, 1998; Laken and Leonard, 2001; Vives et al., 2003b). Despite ... a better understanding of apoptosis signaling in BC and FBC in order to develop strategies to delay the onset of apoptosis and concomitantly prolong cell culture. MATERIALS AND METHODS Cell ... (Mercille and Massie, 1994), cell death in both BC and FBC was due mainly to Figure 1. Cell growth during BC and FBC of CHO cells. Samples are taken during the exponential , stationary and death...
Ngày tải lên: 15/09/2015, 17:10
Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures
... availability (Gu and Wang, 1998) BIOTECHNOLOGY AND BIOENGINEERING, VOL 89, NO 2, JANUARY 20, 2005 Impact of Culture Viability on N-glycosylation Quality glycosidases... and cell yields on serum ... Previous work by Gu and Wang (1998) and Goldman et al. (1998) showed that an increase in sialidase activity followed viability loss closely in perfusion and stirred-tank CHO cell culture and sialylation ... based on low glutamine/glucose concentrations and its impact on cellular metabolism and, more importantly, the productivity and N-glycosylation quality of a model recombinant glycoprotein, interferon...
Ngày tải lên: 15/09/2015, 17:10
Effect of different levels of zeolite and nitrogen on grain yield and nutrient uptake of maize grown in red soil
... Nitrogen, Grain yield, and N, P, K uptake Article Info Accepted: 04 May 2019 Available Online: 10 June 2019 The present study entitled “Influence of zeolite and nitrogen levels on grain yield and nutrient ... completely randomized design using standard procedures (Rao, 1983) The critical difference was used to evaluate the effects of treatments Results and Discussion Grain yield The grain yield of maize ... grains per row and test weight, which ultimately lead to increase in the grain yield These results were comparable to results obtained by Manikandan and Subramanian (2016) where the grain yield of...
Ngày tải lên: 09/01/2020, 14:17
Chapter 9: Working with Selections and Selection Layers
... box until all dirt and rough lines that may have scanned in disappear and the line art looks how you’d like it to be Click the Move and Transform tab and resize, reposition, and rotate your image ... collection of trees, skies and clouds, and mountains • Pattern: Tones that you can use for the background of a scene These are random types of patterns (animals, flowers, and so on), and you can use them ... collection of trees, skies and clouds, and mountains • Pattern: Tones that you can use for the background of a scene These are random types of patterns (animals, flowers, and so on), and you can use them...
Ngày tải lên: 27/08/2012, 14:31
Tài liệu CLUTCHES AND BRAKES Design and Selection
... Understanding Gear Noise, J Derek Smith Chains for Power Transmission and Material Handling: Design and Applications Handbook, American Chain Association Corrosion and Corrosion Protection Handbook, ... Selection and Application, Duane Tandeske 73 Zinc Handbook: Properties, Processing, and Use in Design, Frank Porter 74 Thermal Fatigue of Metals, Andrzej Weronski and Tadeusz Hejwowski 75 Classical and ... Revised and Expanded, Earl Logan, Jr 86 Vibrations of Shells and Plates: Second Edition, Revised and Expanded, Werner Soedel 87 Steam Plant Calculations Manual: Second Edition, Revised and Expanded,...
Ngày tải lên: 20/10/2012, 09:58
Improvement of recruitment and selection process in HKT Consultant Jsc
... by choosing the best pool of candidates quickly and cost efficiently But successful recruitment and selection can be costly and time-consuming Still, recruitment and selection is crucial for a ... Administration and Human Resources, IT, and Finance and Accounting Now, HKT mainly does business in two fields including implementation of IT and modern management models in business and resesearch ... more helpful, higher quality and … simplest Better, faster and … cheaper More comfortable, easier, saving actions and … reflections More convenient, logic, more elegant and … more splendid” Nguyễn...
Ngày tải lên: 24/07/2013, 15:36
Application and selection
... orchestral, and military band, on trombone, fiddle, tin whistle, mandolin, and bodhran …” If Irish music be the food of medicine, play on But that was not all, for he continued: “I also enjoy boxing and ... medicine and then those subjects needed for university matriculation, usually English language and mathematics All attempts at examinations should be entered and clearly separated The date and number ... initiative Other activities, such as hobbies, music, drama, and sport, indicate a willingness and ability to acquire intellectual and practical skills and to participate, characteristics useful in life...
Ngày tải lên: 01/11/2013, 09:20
Clutches and brakes design and selection P1
... processes through overhead cranes, hoists, and industrial brakes and clutches and in farm and garden tractors Some or all of the following materials, and others, that are now necessary to produce ... friction and wear of Polytetrafluoroethylene-based composites Wear 79: 221–234 Anderson, J C (1986) The wear and friction of commercial polymers and composites In: Friedrich, K., ed Friction and Wear ... manufacture of brake linings and clutch facings for both wet (oil bath) and dry clutch applications In dry brake and clutch applications, a flexible, nonwoven form can withstand dynamic pressure up...
Ngày tải lên: 09/11/2013, 03:15
Tài liệu Clutches and brakes design and selection P2 doc
... pressure between the drum and the lining, and w represents the width of the band Upon simplifying equations (1-1) and (1-2) and remembering that as the element of band length approaches zero, ... flexible enough for use in a band brake and has the limiting temperature and pressure capability Thus, use A = 0.4 and equation (1-10) to find that at the maximum radius the band width should be given ... which ~ represents the safety factor and j represents the yield stress of the steel band Substitution of these values along with w and F into equation (4-2) yields t = 0.065 in Finally, from equation...
Ngày tải lên: 17/12/2013, 11:15
Tài liệu Clutches and brakes design and selection P3 docx
... Chapter and that at the upper limit, 1=A ¼ Mf =ðAMp Þ Consequently, the ordinates on the right-hand sides of the graphs in Figures and are the reciprocals of the ordinates on the left-hand sides ... this will yield a satisfactory shoe If it does, the longer band will aid in cooling and may have a longer life The required activation moment Ma may be found from equation (3.9) after A and B have ... an advancing right-hand screw), and ccw indicates counter-clockwise rotation From Figure it follows that dMf ¼ ðAwrp d fÞðr À R cos fÞ ð2-1Þ This is the negative of the integrand in equation (1-10)...
Ngày tải lên: 17/12/2013, 11:15
Tài liệu Clutches and brakes design and selection P4 pdf
... Upon substituting for u1 and u2 in equation (1-8) and recalling equations (1-6) and (1-7) we find that T 4A sinðf0 =2Þ R ¼ ¼A Fs r f0 þ sin f0 r ð1-11Þ where the right-hand side has already been ... Dekker, Inc ð2-12Þ Linearly Acting External and Internal Drum Brakes 77 Substitution for Mp and Mf from equations (2-7) and (2-11) into equation (2-12) yields h pmax r f0 Rrw ðf0 þ sin f0 Þsin a ... symmetrically pivoted and if equation (1-7) holds, the applied torque is given by T ¼ ARFs ð1-12Þ II BRAKING TORQUE AND MOMENTS FOR SYMMETRICALLY SUPPORTED INTERNAL SHOES Pressure p and braking torque...
Ngày tải lên: 17/12/2013, 11:15
Tài liệu Clutches and brakes design and selection P5 docx
... Elimination of k and y from equations (1-1) and (1-2) yields p ¼ pmax rj r ð1-3Þ With the lining pressure known, we may now calculate the required axial force from Z F ¼ p da ð1-4Þ A and the resulting ... to ri and setting the derivative to zero yields ro ri ¼ pffiffiffi ð3-4Þ as the theoretically optimum value of ri, corresponding to torque and activating force given by T ¼ pffiffiffi Apmax kr3 o 3 and ð3-5Þ ... pad and caliper combination which provides the largest heat sink, shown in Figure and the shortest life for that with the smallest heat sink, shown in Figure 10 III ANNULAR CONTACT DISK BRAKES AND...
Ngày tải lên: 17/12/2013, 11:15
Tài liệu Clutches and brakes design and selection P6 ppt
... designed and tested for a range of vehicle sizes, from tractors and trailers to subcompact automobiles [1] Both the large and small sizes used a folded cone design, as illustrated in Figures and 6, ... equations (1-6) and (1-9) reduce to the correct expressions for the torque and activation force for an annular contact disk brake with a single friction surface Unlike plate clutch and brakes, it ... Figures and It is Figure that is directly useful in the design of cone brakes and clutches, because we find from equation (1-19) that the regions to the left of the solid curve (regions and 4)...
Ngày tải lên: 17/12/2013, 11:15
Tài liệu Clutches and brakes design and selection P7 pdf
... configuration of the OM and the coil housing, as shown in Figures and Both the shear and tensile stresses in these links resist relative motion between the IM and the OM and so transmit torque ... vector P and magnetization vector M are defined by P ¼ D À eo E and B ¼ Ao ðH þ M þ Mo Þ ð1-4Þ because both P and M vanish in free space The last two of equations (1-2) are replaced by D ¼ eE and H¼ ... density (coulombs/meter3) and constants qo and Ao denote the electric and magnetic permeabilities of free space, respectively In the MKS system, the units of qo are farads/meter and the units of Ao...
Ngày tải lên: 17/12/2013, 11:15
Tài liệu Clutches and brakes design and selection P8 ppt
... moment of inertia Ii, n weights Wi and their elevation changes, and including nonzero values of velocity vi, and angular rotation Ni III BRAKING AND CLUTCHING TIME AND TORQUE Work done by a brake ... such as band, external linear, annular caliper, and annular disk brakes To choose among these, recall equation (1-10) from Chapter 1, equation (2-1) from Chapter 4, and equations (1-7) and (3-5) ... coefficient of 0.3, and set the width for the band, the linearly acting drum brake, and the externally pivoted brake to cm Limit the maximum lining pressure for the band brake and for the externally...
Ngày tải lên: 17/12/2013, 11:15