regulatory role of mitochondria in oxidative stress and atherosclerosis

... concen- trations (5 and 10 mm)ofMg 2+ inhibited the DNA cleavage activity (Fig. 4C, lane 4 and 5) and weak inhi- bition was observed even in 1 mm of Mg 2+ (compare lane 2 and 3 in Fig. 4C), indicating a different ... caspase-8- and caspase-9- like activities, which appear to be involved in the final resorption of the parental macronucleus during PND in T. thermophila, and suggested the involvement of mitochondria in ... becomes acidic in the final step of PND [22,23]. DePsipher staining of the macronucleus appeared initially during the stage of autophagosome formation, and persisted until resorp- tion of the parental...

Ngày tải lên: 20/02/2014, 03:20

... A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. CYP2E1 causes apoptosis of cardiomyocytes ... College, Beijing, China 2 Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Institute of Laboratory Animal Science, Chinese Academy of Medical ... overexpression of CYP2E1 both in vivo and in vitro is associated with several cellular markers of oxidative stress, including products of lipid peroxi- dation (e.g. 4-hydroxynonenal and malondialdehyde), as...

Ngày tải lên: 22/03/2014, 16:20

... examines the role of language in reducing poverty in a community by enhancing its self esteem, image, identity, and encouraging participation in education and civic society. It argues that in ... issues of education involving adults, but it also directly and indirectly influences the education of children because the teaching and learning of children is supervised by the older members of ... enables it to disseminate information, encourage participation in the education and economy of the communities. To achieve its goal of disseminating useful information and eradicating poverty the...

Ngày tải lên: 05/11/2012, 16:27

Ngày tải lên: 18/02/2014, 11:20

... deletion abolished binding of an U1snRNP molecule in this position and activated a 3¢ss lying 12 nucleotides upstream of this element. In the last case, binding of hnRNP E1 and U1snRNP to a silencer ... Italy Introduction Towards the end of the 1970s, in the beginning of pre-mRNA splicing research [1,2], defining exons and introns was essentially based on observing the final composition of the ... recognition in the normal splicing process [55]. First, U1snRNP binding to an intronic splicing processing element has been found to inhibit pathological pseudoexon inclusion in intron 20 of the ATM...

Ngày tải lên: 06/03/2014, 09:22

... subset of different L types) and imposes very few restrictions on it. An interpretation of E as an index of embodied quality in different types and vintages of labor, fixed once and for all and independent ... i earnings, aggregating and computing the percentage change. EDUCATION IN PRODUCTION FUNCTIONS AND GROWTH ACCOUNTING 99 TABLE 9 Regression Coefficients of the Logarithm of income on Schooling and of ... level, the issue of the quality of labor is the issue of the measurement of labor input in constant prices and a question of correct aggregation. It is standard national-income accounting practice 1...

Ngày tải lên: 06/03/2014, 21:20

... localization sig- nal of p53 and its phosphorylation involves S and G 2 ⁄ M cyclin-dependent kinases [21]. Our results now indicate that in protein derived from BVEs, an intact serine resi- due at ... many aspects in common and are linked in the regulation and function of p53 [4,5,22]. The interplay between phosphorylation and acetylation of p53 at residues K373 to K382 is very interesting. These ... recombinant p53 proteins were expressed in Sf9 insect cells by using the baculoviral system and were purified as described previously [15]. Both human and murine p53 proteins were studied and included...

Ngày tải lên: 30/03/2014, 16:20

Ngày tải lên: 11/08/2014, 03:20

Ngày tải lên: 11/08/2014, 06:22

Ngày tải lên: 13/08/2014, 09:21

Ngày tải lên: 13/08/2014, 19:20

Ngày tải lên: 13/08/2014, 20:21

Ngày tải lên: 13/08/2014, 21:21

Ngày tải lên: 02/11/2014, 00:48

... significant insights into the roles and mechan- isms of PM 2.5 -associated physiology and pathology. In summary, our data demonstrate the important effects of PM 2.5 exposure on oxidative stress and ... TEM images of iBAT (Arrows point to mitochondria) . E: The analysis of mitochondrial number per field in the eWAT and iBAT. F: The analysis of mitochondrial area per field in the eWAT and iBAT. ... shows representative TEM images of mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F). In the PM 2.5 -exposed group,...

Ngày tải lên: 06/03/2014, 19:20

... at the EcoRI and BglII sites of pADH10. The resulting plasmid, named pDHEB, contains the gene encoding DCH lacking the C-terminal region, in which Asp and His residues involved in the catalytic ... expected to result from insertion of a single copy of the Km R gene. 3,4-Dihydrocoumarin-hydrolysing activity, monochloro- dimedon-brominating activity, and peracetic acid-degrading activity were compared ... Comparison of the enzyme activities of the A. calcoaceticus dchD strain and parent strain F46. Preparation of cell-free extracts and determination of each enzyme activity were performed as described in the...

Ngày tải lên: 17/03/2014, 09:20

... PARP inhibitor, on oxidative stress- induced changes in D. discoideum development. Interestingly, oxidative stress resulted in PARP activation within 5 min that was inhibited by benzamide. Oxidative ... development. HA-induced oxidative stress activates PARP within 5 min (Fig. 4A,B), and its role during oxidative stress is further confirmed by the use of low concentrations of benzamide. Preincubation of cells ... the response of this organism to oxidative stress and the role of PARP during development. We used hydroxylamine (HA) to induce in situ generation of H 2 O 2 and monitored the effect of benzamide,...

Ngày tải lên: 23/03/2014, 07:20

... [41], and human U87-MG cell line, a kind gift of Professor C. Martini (Department of Psychiatry, Neurobiology, Pharmacology and Biotechnol- ogy, University of Pisa, Italy), were maintained under ... trypsin treatment and stained for 30 min at 37 °C in fresh medium containing JC1 (final concentration of 10 lgÆmL )1 ). Cells were then washed twice with PBS, suspended in 500 lL of PBS and analyzed ... ultrastructural evidence of membrane rup- ture and were also negative to propidium iodide stain- ing (data not shown), indicating that the plasma membrane is maintained intact, allowing us to exclude primary...

Ngày tải lên: 29/03/2014, 09:20

... response of the organism to particle-induced oxida- tive stress. The observed increase of reducing species in urine would mirror an increased level in blood originat- ing from a response to oxidative ... within-shift variation of 8OHdG (% of initial value) and within-shift reduced species (% of initial value) for smokers and non smokers. Sauvain et al. Journal of Occupational Medicine and Toxicology ... 8OHdG and reducing species. (a) Correlation between urinary levels of 8OHdG ( in μg/g creatinine) and reduced species (in μW/g creatinine) for all collected samples. (b) Correlation between within-shift...

Ngày tải lên: 20/06/2014, 00:20

Ngày tải lên: 07/08/2014, 23:22