regulatory elements in promoters

Ngày tải lên: 20/06/2014, 01:20

... the DHS in intron 18 is less evident ( Fig. 5B). In contrast, the DHS in intron 18 is more prominent than those in introns 16 and 17 (Fig. 5A) in chromatin from Capan1 cells. The DHS in i ntrons ... increase in the intensity of the DHS fragments in the control samples. In Fig. 7A the forskolin-treated chromatin shows a relative increase in the intensity of the DHS10a (1716 + 13.2 kb) appearing ... of the DHS suggested a role for the DHS in introns 2 and 18 in CFTR expression in some pancreatic duct cells. Finally, regulatory elements at the DHS in intron s 1 0 a nd 18 may contribute to...

Ngày tải lên: 08/03/2014, 10:20

... obtained using the Cdc2 and Cdc25C promoters. In G 0 , proteins appear to bind to the CDE ⁄ CHR, as monitored by in vivo footprinting. Accord- ing to these early experiments all binding is lost in ... expression in G 2 phase and in mitosis. Promoters of these genes are repressed by pro- teins binding to CDE ⁄ CHR elements in G 0 and G 1 phases. Relief from repression begins in S phase and continues into ... the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin- associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48. Furthermore, A-Myb and B-Myb were present in immunoprecipitations for Lin-9, Lin-37 and Lin-54 [90,92]....

Ngày tải lên: 16/02/2014, 09:20

... regulatory element of the GAL genes in the nucleus. The regulatory protein may bind to the upstream regulatory element constituting a protein– DNA interaction [4–6]. The availability of the regulatory proteintointeractwithDNAmaydependonitsactivity, which ... protein, implying dimerization. The upstream regulatory element itself may be present in m ultiple c opies representing variable binding sites. Furthermore, the status of one binding site could in uence ... genes through i ts N -terminal DNA binding site. Gal80p inhibits the transcriptional activity of Gal4p b y binding to its 2 8 amino acid region at the carboxyl terminal [25–27]. In vitro studies have...

Ngày tải lên: 07/03/2014, 16:20

... some Ž. trace elements Co, Pb and Zn the extractable concentration and stratification was linked to OM and pH. For other elements the behavior observed in our experiment could not be explained. Surface concentrations ... Aires, ´´ Argentina Received 20 September 1998; accepted 21 March 1999 Abstract Because of crop production intensification, we hypothesize that concentrations of trace elements will increase and stratify in ... trace Ž. elements Co, Pb and Zn the extractable concentration and stratification was linked to OM and pH. For other elements the behavior observed in our experiment could not be explained. Surface...

Ngày tải lên: 15/03/2014, 23:22

... phos- phorylation of the N-terminal threonines in both 4E-BP1 and 4E-BP2 (which is not very prone to inhi- bition by rapamycin). In contrast, the TOS motif is required for the insulin-induced phosphorylation ... mutating the pro- line to alanine (to give the RAIA mutant) causes a defect in the basal and insulin-stimulated phosphoryla- tion of 4E-BP1 [25]. We also tested the proline to valine mutation in ... diminished the basal level of phosphorylation, but still permitted some induction of phosphorylation by insulin. Mutation of the arginine to glutamine (QAIP; Fig. 2B), threonine or asparagine (both...

Ngày tải lên: 16/03/2014, 06:20

... further reading of the text  Conveying an advertising message in the shortest possible form  Evoking in consumer’s mind a desire of possessing a given product  Summarising and summing up the ... you doing”. In fact, this kind of greetings is very rude and impolite in western countries, for it is an interference of privacy. Instead we’d better translate them into “hello” “good morning” ... greeting, naming, appreciating, praising, apologizing, departing and making telephone calls. In Vietnam, when we meet each other in the street, we always greet like this “where are you going” or...

Ngày tải lên: 18/03/2014, 10:15

... could bind even in the absence of nonspecific DNA showing that it has a high binding affinity enabling it to compete with the nonspecific DNA-binding proteins present in the extract. Regulatory proteins ... binding protein that becomes totally inactivated within 15 min of heat treatment at 47 °C [34], junRP retains its DNA-binding activity, although at a relatively low level, even when the binding ... kDa in UV cross-linking studies whereas the purified protein is only  40 kDa, suggesting that it binds only as a dimer. Absence of an  40 kDa DNA-protein adduct in UV cross-linking studies indicates...

Ngày tải lên: 23/03/2014, 20:22

Ngày tải lên: 28/03/2014, 15:20

... species whilst retaining the parameters of the former. The in silico re-engineering study indicated that the leaky behavior in response to glucose inhibition was also obtained in S. cerevisiae ... genes with one (full line, f 1p ) and two (broken line, f 2p ) binding sites for varying KlGal4p t concentration in a K. lactis mutant strain lacking KlGAL80. GAL system in K. lactis V. R. Pannala ... promoter for the binding of KlGal4p, resulting in an autoregulatory circuit which causes a two- to five-fold increase in KlGal4p concentration in the presence of lactose ⁄ galactose. This increase is...

Ngày tải lên: 29/03/2014, 09:20

... the information in two text spans may be in contrast; and the information in one text span may provide background for the information presented in another text span. Figure 2 displays in the ... question? c) Are the main points in my essay clearly stated? d) Do the main points in my essay relate to my original thesis statement? If these questions are expressed in general terms, they ... Often in life we experience a conflict in choosing between something we "want" to do and something we feel we "should" do. In your opinion, are there any circumstances in...

Ngày tải lên: 31/03/2014, 04:20

Ngày tải lên: 08/04/2014, 10:37

Ngày tải lên: 12/05/2014, 23:05

Ngày tải lên: 01/06/2014, 10:54

Ngày tải lên: 18/06/2014, 18:20

Ngày tải lên: 19/06/2014, 22:20

... slight increase in serum Cu and a signficant increase in urine Cu levels in patients suffering from myocar- dial infarction (MI) (15). Reunanen et al. (1996) found increased levels of serum Cu in ... eastern Finnish men, in which high levels of serum ferritin and dietary Fe intake were positively associated with the incidence of myocardial infarction. Ascherio et al. (1994) reported an increased ... important in preventing the peroxidation of LDL and this action could be paramount in the prevention of atherosclerosis. As lypophilic molecules, vitamin E and beta-carotene are incorporated into...

Ngày tải lên: 25/10/2012, 10:51