... the DHS in intron 18 isless evident ( Fig. 5B). In contrast, the DHS in intron 18 ismore prominent than those in introns 16 and 17 (Fig. 5A) in chromatin from Capan1 cells. The DHS in i ntrons ... increase in the intensity of theDHS fragments in the control samples. In Fig. 7A theforskolin-treated chromatin shows a relative increase in the intensity of the DHS10a (1716 + 13.2 kb) appearing ... of theDHS suggested a role for the DHS in introns 2 and 18 in CFTR expression in some pancreatic duct cells. Finally, regulatory elements at the DHS in intron s 1 0 a nd 18 maycontribute to...
... obtainedusing the Cdc2 and Cdc25C promoters. In G0, proteins appear tobind to the CDE ⁄ CHR, as monitored by in vivo footprinting. Accord-ing to these early experiments all binding is lost in ... expression in G2phase and in mitosis. Promoters of these genes are repressed by pro-teins binding to CDE ⁄ CHR elementsin G0and G1phases. Relief fromrepression begins in S phase and continues into ... theMuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48.Furthermore, A-Myb and B-Myb were present in immunoprecipitations for Lin-9, Lin-37 and Lin-54[90,92]....
... regulatory element of the GALgenes in the nucleus. The regulatory protein may bind tothe upstream regulatory element constituting a protein–DNA interaction [4–6]. The availability of the regulatory proteintointeractwithDNAmaydependonitsactivity,which ... protein, implyingdimerization. The upstream regulatory element itself maybe present in m ultiple c opies representing variable bindingsites. Furthermore, the status of one binding site could in uence ... genesthrough i ts N -terminal DNA binding site. Gal80p inhibitsthe transcriptional activity of Gal4p b y binding to its 2 8amino acid region at the carboxyl terminal [25–27]. In vitrostudies have...
... someŽ.trace elements Co, Pb and Zn the extractableconcentration and stratification was linked to OMand pH. For other elements the behavior observed in our experiment could not be explained. Surfaceconcentrations ... Aires,´´ArgentinaReceived 20 September 1998; accepted 21 March 1999AbstractBecause of crop production intensification, we hypothesize that concentrations of trace elements will increase andstratify in ... traceŽ. elements Co, Pb and Zn the extractable concentration and stratification was linked to OM and pH. For other elements the behavior observed in our experiment could not be explained. Surface...
... phos-phorylation of the N-terminal threonines in both4E-BP1 and 4E-BP2 (which is not very prone to inhi-bition by rapamycin). In contrast, the TOS motif isrequired for the insulin-induced phosphorylation ... mutating the pro-line to alanine (to give the RAIA mutant) causes adefect in the basal and insulin-stimulated phosphoryla-tion of 4E-BP1 [25]. We also tested the proline tovaline mutation in ... diminished the basal level ofphosphorylation, but still permitted some induction ofphosphorylation by insulin. Mutation of the arginineto glutamine (QAIP; Fig. 2B), threonine or asparagine(both...
... further reading of the text Conveying an advertising message in the shortest possible form Evoking in consumer’s mind a desire of possessing a given product Summarising and summing up the ... you doing”. In fact, this kind of greetings is very rude and impolite in western countries, for it is an interference of privacy. Instead we’d better translate them into “hello” “good morning” ... greeting, naming, appreciating, praising, apologizing, departing and making telephone calls. In Vietnam, when we meet each other in the street, we always greet like this “where are you going” or...
... couldbind even in the absence of nonspecific DNA showing thatit has a high binding affinity enabling it to compete with thenonspecific DNA-binding proteins present in the extract. Regulatory proteins ... binding protein that becomestotally inactivated within 15 min of heat treatment at 47 °C[34], junRP retains its DNA-binding activity, although at arelatively low level, even when the binding ... kDa in UV cross-linking studies whereas the purified protein is only 40 kDa, suggesting that it binds only as a dimer. Absenceof an 40 kDa DNA-protein adduct in UV cross-linkingstudies indicates...
... species whilstretaining the parameters of the former. The in silicore-engineering study indicated that the leaky behavior in response to glucose inhibition was also obtained in S. cerevisiae ... genes with one (full line, f1p) and two (broken line,f2p) binding sites for varying KlGal4ptconcentration in a K. lactis mutant strain lacking KlGAL80.GAL system in K. lactis V. R. Pannala ... promoter for the bindingof KlGal4p, resulting in an autoregulatory circuitwhich causes a two- to five-fold increase in KlGal4pconcentration in the presence of lactose ⁄ galactose. Thisincrease is...
... the information in two text spans may be in contrast; and the information in one text span may provide background for the information presented in another text span. Figure 2 displays in the ... question? c) Are the main points in my essay clearly stated? d) Do the main points in my essay relate to my original thesis statement? If these questions are expressed in general terms, they ... Often in life we experience a conflict in choosing between something we "want" to do and something we feel we "should" do. In your opinion, are there any circumstances in...
... slight increase in serum Cu and a signficant increase in urine Cu levels in patients suffering from myocar-dial infarction (MI) (15). Reunanen et al. (1996) found increased levels of serum Cu in ... eastern Finnish men, in which high levels of serum ferritin and dietary Fe intake were positively associated with the incidence of myocardial infarction. Ascherio et al. (1994) reported an increased ... important in preventing the peroxidation of LDL and this action could be paramount in the prevention of atherosclerosis. As lypophilic molecules, vitamin E and beta-carotene are incorporated into...
... from the support and resources of a continuing education program. In designing and maintaining the sanitation program, keep a few keypoints in mind:1. Be a regulatory watcher.2. A beverage sanitation ... down Regulatory Lane are the Drinking Water regulations,21 CFR Part 129. Again, comparable international standards exist in theCodex Alimentarius. “Processing and Bottling of Bottled Drinking ... steps: washing, peeling, trimming; heat blanch-ing; batters and breaders; Þlling, assembling, packaging; dry products; andacid and acidiÞed products.Finally, the section on Warehousing requires...
... the first structure ofan ACMSD in a ligand-bound form and can be usedto provide insights into catalysis. In particular, theresidues involved in inhibitor binding can be suggestedto be important ... following insulin injection[21,22], we decided to investigate the effect ofNHCH2CHCOOHNH2L-kynurenineCHOCOOHCOOHNH2Tryptophanα-amino-β-carboxymuconate-ε-semialdehyde (ACMS)α-amino-β-carboxymuconate-ε-semialdehyde ... ACMSD-specific small insertion domain in blue. The DHAP molecule andthe protein residues involved in metal coordination are shown assticks; the Zn2+ion and the solvent molecule bridging the metalcentre...