regulation of plant gads

... Alignment of nucleotide sequence of the 5’- and 3’-termini of mustard GADs 156 32 Prediction of the secondary structure of C-terminal of mGADs with PSIPRED software 158 33 Hydropathy profile of cDNA-derived ... 2.3 Effects of PAs on oxidative stress 39 2.4 GABA shunt pathway 41 2.4.1 GAD 43 2.4.1.1 Sequence characteristics of plant GADs 43 2.4.1.2 GAD expression 44 2.4.1.3 Regulation of plant GADs 46 2.4.2 ... of mGAD2 149 29 Comparison of the deduced amino acid sequence between mGADs and other plant GAD homologs 152 30 Comparison of the deduced amino acid sequence between mGAD2 and GADs from non-plant...

Ngày tải lên: 12/09/2015, 10:19

... of desired product PCR: make large amounts of a particular sequence • The number of molecules of the DNA fragment between the primers increases about 2-fold with each cycle • For n = number of ... Polymerase Chain Reaction • Requires knowledge of the DNA sequence in the region of interest • As more sequence information becomes available, the uses of PCR expand • With appropriate primers, one ... vector: pUC lacZ mulitple cloning sites pUC ApR ColE1 origin of replication Lac+, or blue colonies on X-gal in appropriate strains of E coli High copy number foreign DNA lacZ pUC recombinant ApR...

Ngày tải lên: 13/03/2014, 18:39

... http://www.biomedcentral.com/1471-2229/7/66 Figure profile of the assembly of cassava ESTs Cluster Cluster profile of the assembly of cassava ESTs The graph presents the number of clones per assembled scaffold; ... transcripts of a total of 7796 distinct genes, with alternative transcripts of about 26% of them To find the number of new transcripts captured in this library, relative to the number of expressed ... production of cyanogen-free transgenic cassava plants [22,23] One tool that may assist both, the characterization of a plant expressed genes and the isolation of nucleotide sequences of genes with...

Ngày tải lên: 12/08/2014, 05:20

... lack of standardized criteria for laboratory diagnostics of chronic C pneumoniae infection as well as contradictory information about distribution of the pathogen throughout of the tissues of human ... µl of DNA was added to reaction mixture containing 1x PCR buffer (Silex, Moscow, RF) containing 10 mM Tris-HCl, pH 8,3 , 2.5 mM MgCl2, 15 pmol of each primer, 200 µM of each of dNTPs and U of ... diagnostics of C pneumoniae infection (8, 9) However, changed antigenic profile of C pneumoniae during persistent colonization in human tissues (10, 11) undermines the diagnostic value of serological...

Ngày tải lên: 26/10/2012, 08:57

... IPTG; S2, supernatant of the homogenate of E coli BL21(DE3)/pETHRT2 with IPTG; PC, pellet of the homogenate of E coli BL21(DE3)/pET32b without IPTG; P1, pellet of the homogenate of E coli BL21(DE3)/pETHRT1 ... that of yeast dolichols (C80–C100; 1.1–1.3 kDa) GPC analysis of the rubber material synthesized by the action of purified HRT2 in the absence of WBP indicated the production of a small amount of ... particles (WBP) of fresh Hevea latex These results suggest that the HRT2 protein catalyzes the synthesis of new rubber molecules with the active involvement of a number of factors in WBP of fresh Hevea...

Ngày tải lên: 07/03/2014, 21:20

... revealed the amounts of RNA loaded per lane The sizes of an RNA marker are indicated the cloning of the 5¢ and 3¢ end of the clone, respectively Analysis of the nucleotide sequences of the newly obtained ... [24] Hence, analysis of the regulation of ppod1 expression should shed some more light on the mechanisms of pattern formation in the foot of hydra and will be the subject of further investigations ... the timing of the appearance and the localization of the mRNA was also in accordance with the peroxidase protein Fig 10 Kinetics of the reappearance of ppod1 in foot regenerating tissue of H vulgaris...

Ngày tải lên: 08/03/2014, 10:20

... medium and showed slow growth on YPD as in the case of disruptants of most other mrp genes In the case of MRPS8, it was indicated that the addition of a short peptide to the C-terminus caused poor ... synthesis lead to the loss of mitochondrial genome Recent analysis of mutants indicated that availability of isoleucine in the cell might be related to the stability of the mitochondrial genome ... analysis of the proteins associated with yeast mitoribosome isolated by affinity purification using the S-tag attached to Mrp4 protein led to the identification of 27 mrps of the small subunit and 22 of...

Ngày tải lên: 17/03/2014, 10:20

... yield of the apo-BCCPD67 K117L mutant was  15 mg of protein per litre of cell culture Biochemical properties of BPL Activity assays were performed with BPL by measuring the incorporation of [14C]biotin ... formation of a fragment of  21 kDa Chymotrypsin digestion also produced an array of smaller peptide fragments We found that only 34% of total BPL remained after trypsin cleavage in the absence of substrates ... of 37 kDa (Fig 6, inset) Electrospray analysis of the complex gave a molecular mass of 37 200 ± 200 Da which agrees well with the predicted mass of a : heterodimer Discussion The attachment of...

Ngày tải lên: 31/03/2014, 07:20

... three-dimensional structures of certain domains of nitric oxide synthase isoforms are known [53±57], we are not aware of the three-dimensional structure of the calmodulin binding sites of any isoform being reported ... 82±101 of caveolin-1 in the form of glutathione S-transferase fusion proteins have been shown to attenuate the activities of all three isoforms of NOS [62] A novel 10-kDa protein inhibitor of nNOS, ... small peptides (one of which mirrors part of the sequence of the calmodulin binding site of rat nNOS) preferentially interact with Ca2+calmodulin, impeding the interaction of Ca2+calmodulin with...

Ngày tải lên: 31/03/2014, 15:20

... the roof If additional roof deflection is mm or less, the isolator should be selected for 10 times the additional roof deflection If additional roof deflection is over mm, supplemental roof stiffening ... class of equipment Certain spaces may dictate higher levels of isolation For example, bar joist roofs may require a static deflection of 38 mm over factories, but 64 mm over commercial office ... class of equipment Certain spaces may dictate higher levels of isolation For example, bar joist roofs may require a static deflection of 38 mm over factories, but 64 mm over commercial office...

Ngày tải lên: 31/03/2014, 18:20

... concentrations of relevant RNAs by drug-induced overexpression of genes of interest, and (3) inhibition of protein synthesis by inhibitors, resulting in extended transcription of the early genes of mammalian ... significance of these molecules In addition, one can examine differential gene expression of other genes in the presence and absence of the gene of interest by modulating the levels of each gene of interest ... mixture: µL of M Tris-HCl (pH 6.9) (final 50 mM); 4.5 µL of M MgCl2 (final mM); 12 µL of M KCl (final 100 mM); 3.3 µL of M DTT (final mM); 1.2 µL of M (NH4)2SO4 (final 10 mM); 1.2 µL of 10 mM β-NAD+...

Ngày tải lên: 11/04/2014, 01:04

... 20% The direct costs of the production of m3 of raw wood assortments were derived by means of the product of SH/m3 with the wage schedule and coefficient of recalculation of labour costs to direct ... difference of gross yield and own costs of logging Value yield tables of poplar clones were constructed by the method of simulation from the models of assortment yield tables, prices of the assortments ... fusion of models of yield tables and stand assortment tables, models of quality and models of damage to stands Models of yield tables (Petráš, Mecko 2001, 2005) determine the development of mean...

Ngày tải lên: 07/08/2014, 03:22

... the evaluation of three times four plants The growth traits of stem diameter at a height of 0.5 m to the nearest mm and plant height to the nearest 0.1 m were measured at the end of the growing ... the time frame of the main incidence of the rust Stem diameter and the main part of plant height were realized before the onset of the pathogen incidence while the production of storage compounds ... from clones 7, 107, 110, 152 The stability of the intensity of infection of black poplar clones by the rust (the rank of clones according to the intensity of infection) between the particular years...

Ngày tải lên: 07/08/2014, 10:21

... diameter of stand (cm) Fig Proportions of the quality classes of logs of clone Robusta with zero and 40% damage of stems of quality class A Fig Proportions of the quality classes of logs of clone ... classes of logs of clone Robusta with zero and 40% damage of stems of quality class B Fig Proportions of the quality classes of logs of clone I-214 with zero and 40% damage of stems of quality ... 10 Damage of stands 0% 90 VI VI 14 18 22 26 30 34 38 42 46 50 54 58 62 Mean diameter of stand (cm) Proportion of quality classes of the logs (%) 0% 90 Proportion of quality classes of the logs...

Ngày tải lên: 07/08/2014, 10:21

... that ten of twelve isolates were not neutralized by antibodies to any of ALV subgroups including J , and only two isolates were neutralized with a specific serum of HPRS-103, the prototype of subgroup ... of the HPRS-103 strain of J subgroup avian leukosis virus and of a derivative acutely transforming virus Vet Pathol 1997, 34, 127-137 Bai, J., K How es, L N P ayne, and M A Skinner Sequence of ... and transmission of the HPRS-103 strain of avian leukosis virus Leukemia 1992, 6, 1167-1176 Payne, L N., A M Gillespie, and K How es Unsuitability of chicken sera for detection of exogenous ALV...

Ngày tải lên: 07/08/2014, 15:20

... In vitro cholesterol-lowering test: Among isolated 100 strains of LAB, of Streptococcus strains, 11 of Lactobacillus strains and of Bifidobacterium strains were selected as provisional probiotic ... vande Woestyne M, Verstraete W In vitro study of bile-salt hydrolase (BSH) activity of BSH isogenic Lactobacillus plantarum 80 strains and estimation of cholesterol lowering through enhanced BSH ... presence of bile salt These results showed that many LAB have a great cholesterol-lowering activity in the MRS broth regardless to the presence of bile salt (0.3% oxgall) (Table 2) Identification of...

Ngày tải lên: 07/08/2014, 18:20

... individual clones The influence of the genetic background of the mother tree is demonstrated by differences found in plant survival by the mean number of surviving plants/clone (table I) Depending ... this article, we report the micropropagation of late-flushing Q robur from acorns of selected trees To our knowledge, results of ex vitro rooting of micropropagated oak are presented here for ... sterile water times for In each case, half of the embryos of the 11 mother trees were placed on solid Gresshoff and Doy (GD) medium (1972) and woody plant medium (WPM) (Lloyd and McCown, 1980),...

Ngày tải lên: 08/08/2014, 19:21

... (1986) Sources of variation and measures of variability in even-aged stands of plants Ann Bot 58, 757-778 Bisoffi S (1988) Border effects in a multiclonal poplar (Populus spp) plantation Genet ... clonal block planted At the location of Orsay (48°42’N, 02°12’E, Paris at about 280 km SSW of Afsnee) in France, another SRIC plantation was established at the same time in blocks of cuttings ... development of each individual within a particular stand This development may be influenced by other factors, eg, the availability of limiting resources This may be the origin of size hierarchies of individuals...

Ngày tải lên: 08/08/2014, 19:21

... characteristics was collected on representative trees of each of the clones Number of branches, branch length and diameter, and the angles of origin and termination were determined for both proleptic ... measured Dry weight (DW) of proleptic and sylleptic branches, current terminal and the remainder of the stem were determined, as well as DW and leaf area (LA) of leaves of current terminal, proleptic ... the middle of the growing season, 35-40% of the total LA were carried on sylleptic branches in hybrid clones 44-136 and 11-11, respectively The LA on the current terminal of each of the clones...

Ngày tải lên: 09/08/2014, 02:21

... Hors-Cayla [9] and is composed of a total of 38 hamster-bovine cell lines A more complete description of the panel is given in Laurent et al [11] A correlation coefcient of 0.69 was used as the threshold ... standardisation of cattle karyotype held in College Station (Texas) It resulted in the choice of 31 marker genes already mapped in man to permit unambiguous identication and numbering of each bovine ... (Eggen et al., submitted) Clones were pooled in 46 primary superpools of 304 clones each and in secondary pools consisting of pools of plates, columns and rows using a 3D strategy PCR-based screening...

Ngày tải lên: 09/08/2014, 18:21